34 results on '"Basiouni, Shereen"'
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2. Nutritional Disorders in Fattening Turkeys
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El-Wahab, Amr Abd, Chuppava, Bussarakam, Shehata, Awad A., Basiouni, Shereen, Eisenreich, Wolfgang, Hafez, Hafez M., Hafez, Hafez M., editor, and Shehata, Awad A., editor
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- 2024
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3. Influence of COVID-19 on the sustainability of livestock performance and welfare on a global scale.
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Rahman, Md, Islam, Md, Shehata, Awad, Basiouni, Shereen, Hafez, Hafez, Azhar, Esam, Khafaga, Asmaa, Bovera, Fulvia, and Attia, Youssef
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Animal welfare ,COVID-19 ,Economic loss ,Livestock production ,SARS-CoV-2 ,Supply chain ,Sustainability ,Animal Welfare ,Animals ,COVID-19 ,Ecosystem ,Humans ,Livestock ,SARS-CoV-2 - Abstract
Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is currently spreading worldwide. The pandemic has already had significant adverse effects on human civilization, the environment, and the ecosystem at national and global levels. Moreover, the various sectors of the food production chain, particularly agriculture and livestock, have also been significantly affected in terms of production sustainability and economic losses. The global pandemic has already resulted in a sharp drop in meat, milk, and egg production. Restrictions of movement at national and international levels, implemented as a part of control strategies by public health sectors, have negatively impacted business related to the supply of raw materials for livestock farmers and farm outputs, veterinary services, farmworkers, and animal welfare. This review highlights the significant impacts of COVID-19 on the sustainability of livestock performance, welfare on a global scale, and strategies for mitigating these adverse effects.
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- 2022
4. Evolutionary insights into the furin cleavage sites of SARS-CoV-2 variants from humans and animals
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Nagy, Abdou, Basiouni, Shereen, Parvin, Rokshana, Hafez, Hafez M., and Shehata, Awad A.
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- 2021
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5. Immunization of turkeys with a DNA vaccine expressing the haemagglutinin gene of low pathogenic avian influenza virus subtype H9N2
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Shehata, Awad A., Basiouni, Shereen, Ali, Ahmed, Fawzy, Mohamed, Hafez, Hafez M., Ulbert, Sebastian, and Vahlenkamp, Thomas
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- 2020
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6. Development of Salmonella Enteritidis vaccine candidate based on streptomycin independent suppressor and metabolic drift rifampicin resistance-attenuating markers
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Shehata, Awad A., Tarabees, Reda, Elsayed, Mohamed, Wareth, Gamal, and Basiouni, Shereen
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- 2020
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7. Effects of Spirulina platensis and/or Allium sativum on Antioxidant Status, Immune Response, Gut Morphology, and Intestinal Lactobacilli and Coliforms of Heat-Stressed Broiler Chicken
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Attia, Youssef A., primary, Hassan, Reda A., additional, Addeo, Nicola Francesco, additional, Bovera, Fulvia, additional, Alhotan, Rashed A., additional, Al-qurashi, Adel D., additional, Al-Baadani, Hani H., additional, Al-Banoby, Mohamed A., additional, Khafaga, Asmaa F., additional, Eisenreich, Wolfgang, additional, Shehata, Awad A., additional, and Basiouni, Shereen, additional
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- 2023
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8. Alternative to antibiotic growth promoters: beneficial effects of Saccharomyces cerevisiae and/or Lactobacillus acidophilus supplementation on the growth performance and sustainability of broilers’ production
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Attia, Youssef A., primary, Basiouni, Shereen, additional, Abdulsalam, Nisreen M., additional, Bovera, Fulvia, additional, Aboshok, Afaf A., additional, Shehata, Awad A., additional, and Hafez, Hafez M., additional
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- 2023
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9. An overview of the use of bacteriophages in the poultry industry: Successes, challenges, and possibilities for overcoming breakdowns
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Abd-El Wahab, Amr, primary, Basiouni, Shereen, additional, El-Seedi, Hesham R., additional, Ahmed, Marwa F. E., additional, Bielke, Lisa R., additional, Hargis, Billy, additional, Tellez-Isaias, Guillermo, additional, Eisenreich, Wolfgang, additional, Lehnherr, Hansjörg, additional, Kittler, Sophie, additional, Shehata, Awad A., additional, and Visscher, Christian, additional
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- 2023
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10. Anti-Inflammatory and Antioxidative Phytogenic Substances against Secret Killers in Poultry : Current Status and Prospects
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Basiouni, Shereen, Tellez-Isaias, Guillermo, Latorre, Juan D., Graham, Brittany D., Petrone-Garcia, Victor M., El-Seedi, Hesham, Yalcin, Sakine, El-Wahab, Amr Abd, Visscher, Christian, May-Simera, Helen L., Huber, Claudia, Eisenreich, Wolfgang, Shehata, Awad A., Basiouni, Shereen, Tellez-Isaias, Guillermo, Latorre, Juan D., Graham, Brittany D., Petrone-Garcia, Victor M., El-Seedi, Hesham, Yalcin, Sakine, El-Wahab, Amr Abd, Visscher, Christian, May-Simera, Helen L., Huber, Claudia, Eisenreich, Wolfgang, and Shehata, Awad A.
- Abstract
Chronic stress is recognized as a secret killer in poultry. It is associated with systemic inflammation due to cytokine release, dysbiosis, and the so-called leaky gut syndrome, which mainly results from oxidative stress reactions that damage the barrier function of the cells lining the gut wall. Poultry, especially the genetically selected broiler breeds, frequently suffer from these chronic stress symptoms when exposed to multiple stressors in their growing environments. Since oxidative stress reactions and inflammatory damages are multi-stage and long-term processes, overshooting immune reactions and their down-stream effects also negatively affect the animal’s microbiota, and finally impair its performance and commercial value. Means to counteract oxidative stress in poultry and other animals are, therefore, highly welcome. Many phytogenic substances, including flavonoids and phenolic compounds, are known to exert anti-inflammatory and antioxidant effects. In this review, firstly, the main stressors in poultry, such as heat stress, mycotoxins, dysbiosis and diets that contain oxidized lipids that trigger oxidative stress and inflammation, are discussed, along with the key transcription factors involved in the related signal transduction pathways. Secondly, the most promising phytogenic substances and their current applications to ameliorate oxidative stress and inflammation in poultry are highlighted. Simple Summary Chronic stress and inflammation, known also as “secret killers” in animals, can lead to lipid peroxidation, protein oxidation and nitration, DNA damage, and finally apoptosis. This is due to an imbalance between free radical generation and endogenous antioxidant defense, which in turn possess detrimental impacts on the health and performance of animals. In this review, we discuss the mechanistic pathways of oxidative stress and inflammation associated with the main secret killers in poultry, namely heat stress, dysbiosis, leaky gut syndrome, and myco
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- 2023
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11. Anti-Inflammatory and Antioxidative Phytogenic Substances against Secret Killers in Poultry: Current Status and Prospects
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Basiouni, Shereen, primary, Tellez-Isaias, Guillermo, additional, Latorre, Juan D., additional, Graham, Brittany D., additional, Petrone-Garcia, Victor M., additional, El-Seedi, Hesham R., additional, Yalçın, Sakine, additional, El-Wahab, Amr Abd, additional, Visscher, Christian, additional, May-Simera, Helen L., additional, Huber, Claudia, additional, Eisenreich, Wolfgang, additional, and Shehata, Awad A., additional
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- 2023
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12. Phenotypic and Genotypic Characterization of Bacteriocinogenic Enterococci Against Clostridium botulinum
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Shehata, Awad A, Tarabees, Reda, Basiouni, Shereen, Gamil, Mahmoud, Kamal, Ahmed S, and Krüger, Monika
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- 2017
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13. An overview of the use of bacteriophages in the poultry industry: Successes, challenges, and possibilities for overcoming breakdowns
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Abd-El Wahab, Amr, Basiouni, Shereen, El-Seedi, Hesham R., Ahmed, Marwa F. E., Bielke, Lisa R., Hargis, Billy, Tellez-Isaias, Guillermo, Eisenreich, Wolfgang, Lehnherr, Hansjörg, Kittler, Sophie, Shehata, Awad A., and Visscher, Christian
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Microbiology (medical) ,Microbiology ,bacteriophages ,challenges ,alternative antimicrobials ,poultry ,bacteria ,ddc - Abstract
The primary contaminants in poultry are Salmonella enterica, Campylobacter jejuni, Escherichia coli, and Staphylococcus aureus. Their pathogenicity together with the widespread of these bacteria, contributes to many economic losses and poses a threat to public health. With the increasing prevalence of bacterial pathogens being resistant to most conventional antibiotics, scientists have rekindled interest in using bacteriophages as antimicrobial agents. Bacteriophage treatments have also been investigated as an alternative to antibiotics in the poultry industry. Bacteriophages’ high specificity may allow them only to target a specific bacterial pathogen in the infected animal. However, a tailor-made sophisticated cocktail of different bacteriophages could broaden their antibacterial activity in typical situations with multiple clinical strains infections. Bacteriophages may not only be used in terms of reducing bacterial contamination in animals but also, under industrial conditions, they can be used as safe disinfectants to reduce contamination on food-contact surfaces or poultry carcasses. Nevertheless, bacteriophage therapies have not been developed sufficiently for widespread use. Problems with resistance, safety, specificity, and long-term stability must be addressed in particular. This review highlights the benefits, challenges, and current limitations of bacteriophage applications in the poultry industry.
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- 2022
14. Diversity of Coronaviruses with Particular Attention to the Interspecies Transmission of SARS-CoV-2
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Shehata, Awad A., Attia, Youssef A., Rahman, Md. Tanvir, Basiouni, Shereen, El-Seedi, Hesham R., Azhar, Esam I., Khafaga, Asmaa F., and Hafez, Hafez M.
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SARS-CoV-2 ,viruses ,Veterinary medicine ,virus diseases ,COVID-19 ,receptors ,interspecies transmission ,600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::616 Krankheiten ,QL1-991 ,angiotensin-converting enzyme 2 ,SF600-1100 ,One Health ,Zoology - Abstract
In December 2019, the outbreak of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) was reported in China with serious impacts on global health and economy that is still ongoing. Although interspecies transmission of coronaviruses is common and well documented, each coronavirus has a narrowly restricted host range. Coronaviruses utilize different receptors to mediate membrane fusion and replication in the cell cytoplasm. The interplay between the receptor-binding domain (RBD) of coronaviruses and their coevolution are determinants for host susceptibility. The recently emerged SARS-CoV-2 caused the coronavirus disease 2019 (COVID-19) pandemic and has also been reported in domestic and wild animals, raising the question about the responsibility of animals in virus evolution. Additionally, the COVID-19 pandemic might also substantially have an impact on animal production for a long time. In the present review, we discussed the diversity of coronaviruses in animals and thus the diversity of their receptors. Moreover, the determinants of the susceptibility of SARS-CoV-2 in several animals, with special reference to the current evidence of SARS-CoV-2 in animals, were highlighted. Finally, we shed light on the urgent demand for the implementation of the One Health concept as a collaborative global approach to mitigate the threat for both humans and animals.
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- 2022
15. Probiotics, Prebiotics, and Phytogenic Substances for Optimizing Gut Health in Poultry
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Shehata, Awad A., Yalcin, Sakine, Latorre, Juan D., Basiouni, Shereen, Attia, Youssef A., Abd El-Wahab, Amr, Visscher, Christian, El-Seedi, Hesham, Huber, Claudia, Hafez, Hafez M., Eisenreich, Wolfgang, Tellez-Isaias, Guillermo, Shehata, Awad A., Yalcin, Sakine, Latorre, Juan D., Basiouni, Shereen, Attia, Youssef A., Abd El-Wahab, Amr, Visscher, Christian, El-Seedi, Hesham, Huber, Claudia, Hafez, Hafez M., Eisenreich, Wolfgang, and Tellez-Isaias, Guillermo
- Abstract
The gut microbiota has been designated as a hidden metabolic 'organ' because of its enormous impact on host metabolism, physiology, nutrition, and immune function. The connection between the intestinal microbiota and their respective host animals is dynamic and, in general, mutually beneficial. This complicated interaction is seen as a determinant of health and disease; thus, intestinal dysbiosis is linked with several metabolic diseases. Therefore, tractable strategies targeting the regulation of intestinal microbiota can control several diseases that are closely related to inflammatory and metabolic disorders. As a result, animal health and performance are improved. One of these strategies is related to dietary supplementation with prebiotics, probiotics, and phytogenic substances. These supplements exert their effects indirectly through manipulation of gut microbiota quality and improvement in intestinal epithelial barrier. Several phytogenic substances, such as berberine, resveratrol, curcumin, carvacrol, thymol, isoflavones and hydrolyzed fibers, have been identified as potential supplements that may also act as welcome means to reduce the usage of antibiotics in feedstock, including poultry farming, through manipulation of the gut microbiome. In addition, these compounds may improve the integrity of tight junctions by controlling tight junction-related proteins and inflammatory signaling pathways in the host animals. In this review, we discuss the role of probiotics, prebiotics, and phytogenic substances in optimizing gut function in poultry.
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- 2022
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16. An overview of the use of bacteriophages in the poultry industry: Successes, challenges, and possibilities for overcoming breakdowns.
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Wahab, Amr Abd-El, Basiouni, Shereen, El-Seedi, Hesham R., Ahmed, Marwa F. E., Bielke, Lisa R., Hargis, Billy, Tellez-Isaias, Guillermo, Eisenreich, Wolfgang, Lehnherr, Hansjörg, Kittler, Sophie, Shehata, Awad A., and Visscher, Christian
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POULTRY industry ,BACTERIOPHAGES ,POULTRY carcasses ,BACTERIAL contamination ,CAMPYLOBACTER jejuni ,SALMONELLA enterica - Abstract
The primary contaminants in poultry are Salmonella enterica, Campylobacter jejuni, Escherichia coli, and Staphylococcus aureus. Their pathogenicity together with the widespread of these bacteria, contributes to many economic losses and poses a threat to public health. With the increasing prevalence of bacterial pathogens being resistant to most conventional antibiotics, scientists have rekindled interest in using bacteriophages as antimicrobial agents. Bacteriophage treatments have also been investigated as an alternative to antibiotics in the poultry industry. Bacteriophages' high specificity may allow them only to target a specific bacterial pathogen in the infected animal. However, a tailor-made sophisticated cocktail of different bacteriophages could broaden their antibacterial activity in typical situations with multiple clinical strains infections. Bacteriophages may not only be used in terms of reducing bacterial contamination in animals but also, under industrial conditions, they can be used as safe disinfectants to reduce contamination on food-contact surfaces or poultry carcasses. Nevertheless, bacteriophage therapies have not been developed sufficiently for widespread use. Problems with resistance, safety, specificity, and long-term stability must be addressed in particular. This review highlights the benefits, challenges, and current limitations of bacteriophage applications in the poultry industry. [ABSTRACT FROM AUTHOR]
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- 2023
- Full Text
- View/download PDF
17. Poultry Production and Sustainability in Developing Countries under the COVID-19 Crisis: Lessons Learned
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Attia, Youssef A., primary, Rahman, Md. Tanvir, additional, Hossain, Md. Jannat, additional, Basiouni, Shereen, additional, Khafaga, Asmaa F., additional, Shehata, Awad A., additional, and Hafez, Hafez M., additional
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- 2022
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18. Status and Prospects of Botanical Biopesticides in Europe and Mediterranean Countries
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Acheuk, Fatma, primary, Basiouni, Shereen, additional, Shehata, Awad A., additional, Dick, Katie, additional, Hajri, Haifa, additional, Lasram, Salma, additional, Yilmaz, Mete, additional, Emekci, Mevlüt, additional, Tsiamis, George, additional, Spona-Friedl, Marina, additional, May-Simera, Helen, additional, Eisenreich, Wolfgang, additional, and Ntougias, Spyridon, additional
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- 2022
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19. Probiotics, Prebiotics, and Phytogenic Substances for Optimizing Gut Health in Poultry
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Shehata, Awad A., primary, Yalçın, Sakine, additional, Latorre, Juan D., additional, Basiouni, Shereen, additional, Attia, Youssef A., additional, Abd El-Wahab, Amr, additional, Visscher, Christian, additional, El-Seedi, Hesham R., additional, Huber, Claudia, additional, Hafez, Hafez M., additional, Eisenreich, Wolfgang, additional, and Tellez-Isaias, Guillermo, additional
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- 2022
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20. Poultry Production and Sustainability in Developing Countries under the COVID-19 Crisis: Lessons Learned
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Attia, Youssef A., Rahman, Md. Tanvir, Hossain, Md. Jannat, Basiouni, Shereen, Khafaga, Asmaa F., Shehata, Awad A., and Hafez, Hafez M.
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COVID-19 ,developing countries ,sustain ability ,poultry supply chain ,600 Technik, Medizin, angewandte Wissenschaften::610 Medizin und Gesundheit::616 Krankheiten ,smallholder poultry - Abstract
Poultry farming is a significant source of revenue generation for small farmers in developing countries. It plays a vital role in fulfilling the daily protein requirements of humans through meat and eggs consumption. The recently emerged pandemic Coronavirus Disease-19 (COVID-19) impacts the poultry production sector. Although the whole world is affected, these impacts may be more severe in developing countries due to their dependency on exporting necessary supplies such as feed, vaccines, drugs, and utensils. In this review, we have discussed poultry production in developing countries under the COVID-19 crisis and measures to regain the loss in the poultry industries. Generally, due to the lockdown, trade limitations have negatively impacted poultry industries, which might exacerbate global poverty. Coordinated activities have to be taken at the private and government levels to arrange soft loans so that these farms can restore their production and marketing to normal levels. In addition, here, we have focused on the supply of farm input, feed, other raw materials, management system, improved breeding efficiency, veterinary services, and marketing of egg and meat, which have to be ensured to secure a sustainable poultry production chain.
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- 2022
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21. Algae and Their Metabolites as Potential Bio-Pesticides
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Asimakis, Elias, primary, Shehata, Awad A., additional, Eisenreich, Wolfgang, additional, Acheuk, Fatma, additional, Lasram, Salma, additional, Basiouni, Shereen, additional, Emekci, Mevlüt, additional, Ntougias, Spyridon, additional, Taner, Gökçe, additional, May-Simera, Helen, additional, Yilmaz, Mete, additional, and Tsiamis, George, additional
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- 2022
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22. Poult Enteritis and Mortality Syndrome in Turkey Poults: Causes, Diagnosis and Preventive Measures
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Shehata, Awad A., primary, Basiouni, Shereen, additional, Sting, Reinhard, additional, Akimkin, Valerij, additional, Hoferer, Marc, additional, and Hafez, Hafez M., additional
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- 2021
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23. Susceptibility of extended-spectrum β-lactamase (ESBL)-producing Enterobacteriaceae to Roundup
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Krüger, Monika, primary, Basiouni, Shereen, additional, Eder, Ines, additional, and Rodloff, Arne, additional
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- 2021
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24. Mutational spectra of SARS-CoV-2 isolated from animals
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Elaswad, Ahmed, primary, Fawzy, Mohamed, additional, Basiouni, Shereen, additional, and Shehata, Awad A., additional
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- 2020
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25. Characterization of Sunflower Oil Extracts from the Lichen Usnea barbata
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Basiouni, Shereen, primary, Fayed, Marwa A. A., additional, Tarabees, Reda, additional, El-Sayed, Mohamed, additional, Elkhatam, Ahmed, additional, Töllner, Klaus-Rainer, additional, Hessel, Manfred, additional, Geisberger, Thomas, additional, Huber, Claudia, additional, Eisenreich, Wolfgang, additional, and Shehata, Awad A., additional
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- 2020
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26. Isolation and characterization of Salmonella Enteritidis and Salmonella Typhimurium from chicken meat in Egypt
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Tarabees, Reda, primary, Elsayed, Mohamed S. A., additional, Shawish, Reyad, additional, Basiouni, Shereen, additional, and Shehata, Awad A, additional
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- 2017
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27. Phenotypic and Genotypic Characterization of Bacteriocinogenic Enterococci Against Clostridium botulinum
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Shehata, Awad A, primary, Tarabees, Reda, additional, Basiouni, Shereen, additional, Gamil, Mahmoud, additional, Kamal, Ahmed S, additional, and Krüger, Monika, additional
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- 2016
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28. Characterization of Salmonella Enterica Isolated from Poultry Hatcheries and Commercial Broiler Chickens.
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Shehata, Awad A., Basiouni, Shereen, Elrazek, Alaa Abd, Sultan, Hesham, Elraheam Elsayed, Mohamed Sabry Abd, Tarabees, Reda, Talat, Shaimaa, Moharam, Ibrahim, Said, Ahmed, Mohsen, Walaa Atia, Roshdey, Tamer, and Krüger, Monika
- Subjects
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SALMONELLA , *SALMONELLA enterica , *BROILER chickens , *DRUG resistance in microorganisms , *SALMONELLA typhimurium , *SALMONELLA enteritidis , *DRUG resistance in bacteria - Abstract
Salmonella, is a serious pathogen causing disastrous losses in the poultry production and dangerous human infections. This study was aimed to identify Salmonella serotypes and to detect the prominent virulence genes and antimicrobial resistance of the obtained serotypes from samples collected from dead chicken embryos, dead duck embryos and commercial broilers. The pathogenicity of some serotypes as (Salmonella Pullorum, Salmonella Enteritidis, and Salmonella Typhimurium) was investigated in one-day-old commercial layer chicks. A total of 180 cases were collected, after isolation 18 Salmonella isolates were reported to be as follows 7/100(7%), 5/40(12.5%) and 6/40(15%) in broilers, dead chickens, and dead duck embryos, respectively. The most prevalent serotypes were S. Enteritidis, S. Typhimurium, S. Pullorum, S. Vejle, S. Amsterdam, S. Infantis, S. Petersburg, and S. Atakpame. The distribution patterns of virulence genes iroN, cdtB, spaN, invA, and orgA, expressed as follows; 17/18(94.4%), 15/18(83.3%), 14/18(77.7%), 13/18(72.2%), and 12/18(66.7%) of serotypes, respectively. Added to that, the sipV, IpfC, sopB, prgH, and sitC virulence genes were amplified in 7/18(38.8%), 7/18(38.8%), 7/18(38.8%), 5/18(27.7%) and 3/18(16.6%) of isolates respectively. Most of isolates 16/18 (88.9%) expressed multiple antibiotic resistances (MAR) indices ranged from =0.3 =1. Based on the pathogenicity testing, S. Pullorum was the most pathogenic due to the clear signs and a mortality rate of 35%. Hence, the genotypic characterization and continuous monitoring of antimicrobial resistance of Salmonella from poultry are of public health concern to implement effective control strategies against this notorious pathogen. [ABSTRACT FROM AUTHOR]
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- 2019
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29. The modulating effects of polyunsaturated fatty acids on membrane composition and phospholipase D in a canine mast cell line as a model for atopic dermatitis
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Basiouni, Shereen, Fuhrmann, Herbert, Sack, Ulrich, and Veterinärmedizinische Fakultät - Institut für Physiologische Chemie
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Polyunsaturated fatty acids (PUFA), canine atopic dermatitis (CAD), mast cells, exocytosis, cell membrane, Phospholipase D (PLD), lipid raft ,Mehrfach ungesättigte Fettsäuren (PUFA), canine atopischer Dermatitis (CAD), Mastzellen, Exozytose, Zellmembran, Phospholipase D (PLD), Lipid Raft ,food and beverages ,lipids (amino acids, peptides, and proteins) ,ddc:636.089 - Abstract
Polyunsaturated fatty acids (PUFA) have been used with some success in the treatment of canine atopic dermatitis (CAD). Correspondent in vitro studies revealed that PUFA play a crucial role in the exocytosis of mast cells. n3 PUFA such as α-linolenic acid (LNA), eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), as well as the n6 PUFA linoleic acid (LA) have been shown to arrest the secretion of inflammatory mediators. Contrary, the n-6 PUFA arachidonic acid (AA) has been proven to promote the production of mast cell inflammatory mediators. However, we are still lacking a complete picture of the mode of action. The goal of this work was to further characterize the modulatory effects of PUFA supplementation on the plasma membrane lipid composition of mast cells. Furthermore the consequences of a membrane modulation of mast cells by PUFA on the localization and activity on of the membrane bound enzyme phospholipases D (PLD) were investigated. Canine mastocytoma cells (C2) were supplemented with one of the following PUFA: LNA, EPA, DHA, LA or AA. To investigate the influence of PUFA on the lipid composition of membrane microdomains, lipid rafts were separated from non-raft plasma membranes of mast cells for the first time using a detergent-free isolation technique. Results show that PUFA are significantly increased in rafts as well as in non-rafts microdomains (Publication 1). The incorporation of PUFA into the membrane goes along with an increase of the unsaturation status and the fluidity of the membrane. This rise in membrane fluidity may result in a reorganization of membrane signaling molecules and enzymes such as the PLD. To define the impact of a PUFA supplementation on PLD trafficking, C2 were transfected with green fluorescent protein (GFP) fusion plasmids encoding PLD1 or PLD2. Since the transfection ability of the suspension cell line C2 is limited, a special transfection protocol was established, suitable for non-adherent cell lines. Transfection succeeded using chicken egg white as coating material for the cell culture plates. The transfection efficiency rose to 50% versus 5% in uncoated plates. In addition to the obvious increase in the transfection efficiency, the new technique is simple and economic and might be suitable for a wide range of suspension cell lines (Publication 2). Using this optimized protocol the influence of PUFA on the trafficking of PLD isoforms was studied. LNA, EPA, DHA and LA but not AA prevented the stimulation-induced translocation of PLD1 to the plasma membrane. Since the translocation of PLD1 is important for mast cell exocytosis, LNA, EPA, DHA and LA do have an inhibiting effect on the stimulation-induced release of pro-inflammatory mediators. All PUFA tested boosted the total PLD activity. In order to rule out, which PLD isoform was affected by the PUFA, the mast cells were supplemented with DHA or AA in the presence of specific PLD isoform inhibitors. DHA completely abolished the inhibitiory effect of the PLD1 inhibitor but had no effect on the inhibitory effect of PLD2 inhibitor. On the other hand, AA suppressed the inhibitory effect of both PLD1 and PLD2 inhibitor (Publication 3). Taking together, the studies provide a mechanistic base for the role of PUFA in the exocytosis processes of mast cells. PUFA of the n3 and the n6 families impact the lipid composition of membrane microdomains, which in turn lead to a modulation of the physiochemical properties of the membrane. LNA, EPA, DHA and LA suppress the release of inflammatory mediators through their inhibitory action on the stimulation-induced translocation of the PLD1. Contrariwise, AA permits the stimulation-induced migration of PLD1 to the plasma membrane and increases the activity of both PLD isoforms. Therefore, LNA, EPA, DHA and LA but not AA inhibit the release of mast cell inflammatory mediators upon stimulation. Mehrfach ungesättigte Fettsäuren (PUFA) können mit einigem Erfolg zur Behandlung der caninen atopischen Dermatitis (CAD) eingesetzt werden. In vitro-Studien zeigten, dass PUFA eine entscheidende Rolle in der Exozytose von Mastzellen spielen. N-3-PUFA wie α-Linolensäure (LNA), Eicosapentaensäure (EPA), Docosahexaensäure (DHA) sowie die n-6-PUFA Linolsäure (LA) können die Sekretion von Entzündungsmediatoren vermindern. Arachidonsäure (AA) als n-6 mehrfach ungesättigte Fettsäure hingegen fördert die Entzündungsmediatoren-Freisetzung aus den Mastzellen. Eine vollständige Aufklärung der Wirkungsweise fehlt aber weiterhin. Das Ziel dieser Arbeit war eine weitergehende Charakterisierung der modulierenden Effekte einer PUFA-Supplementierung auf die Lipidzusammensetzung der Plasmamembran von Mastzellen. Darüber hinaus wurden die Auswirkungen von PUFA auf die Lokalisation und Aktivität des Membran-gebundenen Enzyms Phospholipase D (PLD) untersucht. Canine Mastozytom-Zellen (C2) wurden mit einer der folgenden PUFA kultiviert: LNA, EPA, DHA, LA oder AA. Um den Einfluss von PUFA auf die Lipidzusammensetzung der Membran-Mikrodomänen zu untersuchen, konnten sowohl Lipid Raft als auch Nicht-Raft Plasmamembran-Anteile von Mastzellen zum ersten Mal mittels einer Detergenzien-freien Isolationsmethode getrennt werden. Hervorzuheben ist, dass PUFA signifikant vermehrt in Raft- sowie in Nicht-Raft Membranmikrodomänen eingelagert werden (Publikation 1). Die Integration von PUFA in die Membran geht mit einer Steigerung der Doppelbindungsanzahl und der Fluidität der Membran einher. Diese Erhöhung der Membranfluidität kann zu einer Reorganisation von membranären Signalmolekülen und Enzymen wie der PLD führen. Um die Auswirkungen einer PUFA-Supplementierung auf den intrazellulären Transport der PLD in C2 zu bestimmen, wurden die Zellen mit PLD1- oder PLD2-codierenden grün fluoreszierenden Protein-(GFP-)Fusionsplasmiden transfiziert. Da die Transfektionsfähigkeit der Suspensions-Zelllinie C2 begrenzt ist, wurde ein für nicht-adhärente Zelllinien geeignetes Transfektionsprotokoll etabliert. Mit Hühnereiweiß als Beschichtungsmaterial für die Zellkultur-Platten stieg die Transfektionseffizienz auf 50% im Vergleich zu 5% bei unbeschichteten Platten. Neben der deutlichen Erhöhung der Transfektionseffizienz ist die neu etablierte Technik einfach durchzuführen sowie wirtschaftlich und kann für eine Vielzahl von Suspension-Zelllinien geeignet sein (Publikation 2). Unter Verwendung dieses optimierten Protokolls wurde der Einfluss von PUFA auf die Translokation der PLD-Isoformen untersucht. LNA, EPA, DHA und LA, nicht aber AA verhindern die stimulationsinduzierte Translokation der PLD1 an die Plasmamembran. Die Translokation der PLD1 ist wichtig für die Mastzell-Exozytose. LNA, EPA, DHA und LA haben hier eine hemmende Wirkung auf die stimulationsinduzierte Freisetzung von proinflammatorischen Mediatoren. Alle getesteten PUFA verstärken die Gesamt-PLD-Aktivität. Um zu unterscheiden, welche PLD-Isoform durch PUFA beeinflusst ist, wurden die Mastzellen mit DHA oder AA in Gegenwart von PLD-Isoform-Inhibitoren supplementiert. DHA hebt die inhibitorische Wirkung des PLD1-Inhibitors vollständig auf, zeigte aber keinen Einfluss auf die hemmende Wirkung des PLD2-Inhibitors. Andererseits unterdrückt AA die hemmende Wirkung des PLD1- als auch des PLD2-Inhibitors (Publikation 3). Zusammenfassend bietet die Studie eine mechanistische Basis für die Rolle von PUFA bei Exozytose-Prozessen von Mastzellen. PUFA der n-3- und n-6-Familie beeinflussen die Lipidzusammensetzung von membranären Mikrodomänen, was wiederum zu einer Modulation der physikalisch-chemischen Eigenschaften der Membran führt. LNA, EPA, DHA und LA verhindern die Freisetzung von Entzündungsmediatoren durch ihre hemmende Wirkung auf die stimulationsinduzierte Translokation der PLD1. Umgekehrt erlaubt AA eine stimulationsinduzierte Migration der PLD1 zur Plasmamembran und steigert die Aktivität der beiden Isoformen der PLD. Somit hemmen LNA, EPA, DHA und LA, aber nicht AA die Freisetzung von Mastzell-Entzündungsmediatoren nach Stimulation.
- Published
- 2013
30. The modulating effects of polyunsaturated fatty acids on membrane composition and phospholipase D in a canine mast cell line as a model for atopic dermatitis
- Author
-
Sack, Ulrich, Veterinärmedizinische Fakultät - Institut für Physiologische Chemie, Basiouni, Shereen, Sack, Ulrich, Veterinärmedizinische Fakultät - Institut für Physiologische Chemie, and Basiouni, Shereen
- Abstract
Polyunsaturated fatty acids (PUFA) have been used with some success in the treatment of canine atopic dermatitis (CAD). Correspondent in vitro studies revealed that PUFA play a crucial role in the exocytosis of mast cells. n3 PUFA such as α-linolenic acid (LNA), eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), as well as the n6 PUFA linoleic acid (LA) have been shown to arrest the secretion of inflammatory mediators. Contrary, the n-6 PUFA arachidonic acid (AA) has been proven to promote the production of mast cell inflammatory mediators. However, we are still lacking a complete picture of the mode of action. The goal of this work was to further characterize the modulatory effects of PUFA supplementation on the plasma membrane lipid composition of mast cells. Furthermore the consequences of a membrane modulation of mast cells by PUFA on the localization and activity on of the membrane bound enzyme phospholipases D (PLD) were investigated. Canine mastocytoma cells (C2) were supplemented with one of the following PUFA: LNA, EPA, DHA, LA or AA. To investigate the influence of PUFA on the lipid composition of membrane microdomains, lipid rafts were separated from non-raft plasma membranes of mast cells for the first time using a detergent-free isolation technique. Results show that PUFA are significantly increased in rafts as well as in non-rafts microdomains (Publication 1). The incorporation of PUFA into the membrane goes along with an increase of the unsaturation status and the fluidity of the membrane. This rise in membrane fluidity may result in a reorganization of membrane signaling molecules and enzymes such as the PLD. To define the impact of a PUFA supplementation on PLD trafficking, C2 were transfected with green fluorescent protein (GFP) fusion plasmids encoding PLD1 or PLD2. Since the transfection ability of the suspension cell line C2 is limited, a special transfection protocol was established, suitable for non-adherent cell lines. Transfection succ, Mehrfach ungesättigte Fettsäuren (PUFA) können mit einigem Erfolg zur Behandlung der caninen atopischen Dermatitis (CAD) eingesetzt werden. In vitro-Studien zeigten, dass PUFA eine entscheidende Rolle in der Exozytose von Mastzellen spielen. N-3-PUFA wie α-Linolensäure (LNA), Eicosapentaensäure (EPA), Docosahexaensäure (DHA) sowie die n-6-PUFA Linolsäure (LA) können die Sekretion von Entzündungsmediatoren vermindern. Arachidonsäure (AA) als n-6 mehrfach ungesättigte Fettsäure hingegen fördert die Entzündungsmediatoren-Freisetzung aus den Mastzellen. Eine vollständige Aufklärung der Wirkungsweise fehlt aber weiterhin. Das Ziel dieser Arbeit war eine weitergehende Charakterisierung der modulierenden Effekte einer PUFA-Supplementierung auf die Lipidzusammensetzung der Plasmamembran von Mastzellen. Darüber hinaus wurden die Auswirkungen von PUFA auf die Lokalisation und Aktivität des Membran-gebundenen Enzyms Phospholipase D (PLD) untersucht. Canine Mastozytom-Zellen (C2) wurden mit einer der folgenden PUFA kultiviert: LNA, EPA, DHA, LA oder AA. Um den Einfluss von PUFA auf die Lipidzusammensetzung der Membran-Mikrodomänen zu untersuchen, konnten sowohl Lipid Raft als auch Nicht-Raft Plasmamembran-Anteile von Mastzellen zum ersten Mal mittels einer Detergenzien-freien Isolationsmethode getrennt werden. Hervorzuheben ist, dass PUFA signifikant vermehrt in Raft- sowie in Nicht-Raft Membranmikrodomänen eingelagert werden (Publikation 1). Die Integration von PUFA in die Membran geht mit einer Steigerung der Doppelbindungsanzahl und der Fluidität der Membran einher. Diese Erhöhung der Membranfluidität kann zu einer Reorganisation von membranären Signalmolekülen und Enzymen wie der PLD führen. Um die Auswirkungen einer PUFA-Supplementierung auf den intrazellulären Transport der PLD in C2 zu bestimmen, wurden die Zellen mit PLD1- oder PLD2-codierenden grün fluoreszierenden Protein-(GFP-)Fusionsplasmiden transfiziert. Da die Transfektionsfähigkeit der Suspensions-Zelllinie C2 b
- Published
- 2014
31. The modulating effects of polyunsaturated fatty acids on membrane composition and phospholipase D in a canine mast cell line as a model for atopic dermatitis
- Author
-
Fuhrmann, Herbert, Sack, Ulrich, Veterinärmedizinische Fakultät - Institut für Physiologische Chemie, Basiouni, Shereen, Fuhrmann, Herbert, Sack, Ulrich, Veterinärmedizinische Fakultät - Institut für Physiologische Chemie, and Basiouni, Shereen
- Abstract
Polyunsaturated fatty acids (PUFA) have been used with some success in the treatment of canine atopic dermatitis (CAD). Correspondent in vitro studies revealed that PUFA play a crucial role in the exocytosis of mast cells. n3 PUFA such as α-linolenic acid (LNA), eicosapentaenoic acid (EPA), docosahexaenoic acid (DHA), as well as the n6 PUFA linoleic acid (LA) have been shown to arrest the secretion of inflammatory mediators. Contrary, the n-6 PUFA arachidonic acid (AA) has been proven to promote the production of mast cell inflammatory mediators. However, we are still lacking a complete picture of the mode of action. The goal of this work was to further characterize the modulatory effects of PUFA supplementation on the plasma membrane lipid composition of mast cells. Furthermore the consequences of a membrane modulation of mast cells by PUFA on the localization and activity on of the membrane bound enzyme phospholipases D (PLD) were investigated. Canine mastocytoma cells (C2) were supplemented with one of the following PUFA: LNA, EPA, DHA, LA or AA. To investigate the influence of PUFA on the lipid composition of membrane microdomains, lipid rafts were separated from non-raft plasma membranes of mast cells for the first time using a detergent-free isolation technique. Results show that PUFA are significantly increased in rafts as well as in non-rafts microdomains (Publication 1). The incorporation of PUFA into the membrane goes along with an increase of the unsaturation status and the fluidity of the membrane. This rise in membrane fluidity may result in a reorganization of membrane signaling molecules and enzymes such as the PLD. To define the impact of a PUFA supplementation on PLD trafficking, C2 were transfected with green fluorescent protein (GFP) fusion plasmids encoding PLD1 or PLD2. Since the transfection ability of the suspension cell line C2 is limited, a special transfection protocol was established, suitable for non-adherent cell lines. Transfection succ, Mehrfach ungesättigte Fettsäuren (PUFA) können mit einigem Erfolg zur Behandlung der caninen atopischen Dermatitis (CAD) eingesetzt werden. In vitro-Studien zeigten, dass PUFA eine entscheidende Rolle in der Exozytose von Mastzellen spielen. N-3-PUFA wie α-Linolensäure (LNA), Eicosapentaensäure (EPA), Docosahexaensäure (DHA) sowie die n-6-PUFA Linolsäure (LA) können die Sekretion von Entzündungsmediatoren vermindern. Arachidonsäure (AA) als n-6 mehrfach ungesättigte Fettsäure hingegen fördert die Entzündungsmediatoren-Freisetzung aus den Mastzellen. Eine vollständige Aufklärung der Wirkungsweise fehlt aber weiterhin. Das Ziel dieser Arbeit war eine weitergehende Charakterisierung der modulierenden Effekte einer PUFA-Supplementierung auf die Lipidzusammensetzung der Plasmamembran von Mastzellen. Darüber hinaus wurden die Auswirkungen von PUFA auf die Lokalisation und Aktivität des Membran-gebundenen Enzyms Phospholipase D (PLD) untersucht. Canine Mastozytom-Zellen (C2) wurden mit einer der folgenden PUFA kultiviert: LNA, EPA, DHA, LA oder AA. Um den Einfluss von PUFA auf die Lipidzusammensetzung der Membran-Mikrodomänen zu untersuchen, konnten sowohl Lipid Raft als auch Nicht-Raft Plasmamembran-Anteile von Mastzellen zum ersten Mal mittels einer Detergenzien-freien Isolationsmethode getrennt werden. Hervorzuheben ist, dass PUFA signifikant vermehrt in Raft- sowie in Nicht-Raft Membranmikrodomänen eingelagert werden (Publikation 1). Die Integration von PUFA in die Membran geht mit einer Steigerung der Doppelbindungsanzahl und der Fluidität der Membran einher. Diese Erhöhung der Membranfluidität kann zu einer Reorganisation von membranären Signalmolekülen und Enzymen wie der PLD führen. Um die Auswirkungen einer PUFA-Supplementierung auf den intrazellulären Transport der PLD in C2 zu bestimmen, wurden die Zellen mit PLD1- oder PLD2-codierenden grün fluoreszierenden Protein-(GFP-)Fusionsplasmiden transfiziert. Da die Transfektionsfähigkeit der Suspensions-Zelllinie C2 b
- Published
- 2014
32. The Influence of Polyunsaturated Fatty Acids on the Phospholipase D Isoforms Trafficking and Activity in Mast Cells
- Author
-
Basiouni, Shereen, primary, Fuhrmann, Herbert, additional, and Schumann, Julia, additional
- Published
- 2013
- Full Text
- View/download PDF
33. High-efficiency transfection of suspension cell lines
- Author
-
Basiouni, Shereen, primary, Fuhrmann, Herbert, additional, and Schumann, Julia, additional
- Published
- 2012
- Full Text
- View/download PDF
34. Polyunsaturated fatty acid supplements modulate mast cell membrane microdomain composition
- Author
-
Basiouni, Shereen, primary, Stöckel, Katja, additional, Fuhrmann, Herbert, additional, and Schumann, Julia, additional
- Published
- 2012
- Full Text
- View/download PDF
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