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31 results on '"Bashuda, Hisashi"'

5. Analysis of therapeutic potential of monocytic myeloid-derived suppressor cells in cardiac allotransplantation

Author

Fujimoto, Keiichi, primary, Uchida, Koichiro, additional, Yin, Enzhi, additional, Zhu, Jun, additional, Kojima, Yuko, additional, Uchiyama, Masateru, additional, Yamamoto, Yasuto, additional, Bashuda, Hisashi, additional, Matsumoto, Ryu, additional, Tokushige, Koji, additional, Harada, Masaki, additional, Inomata, Takenori, additional, Kitaura, Jiro, additional, Murakami, Akira, additional, Okumura, Ko, additional, and Takeda, Kazuyoshi, additional

Published
2021
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9. Auditory stimulation of opera music induced prolongation of murine cardiac allograft survival and maintained generation of regulatory CD4+CD25+ cells

Author

Uchiyama Masateru, Jin Xiangyuan, Zhang Qi, Hirai Toshihito, Amano Atsushi, Bashuda Hisashi, and Niimi Masanori

Subjects
Opera music, Regulatory cells, Cardiac transplantation, Mouse, Surgery, RD1-811, Anesthesiology, RD78.3-87.3
Abstract

Abstract Background Interactions between the immune response and brain functions such as olfactory, auditory, and visual sensations are likely. This study investigated the effect of sounds on alloimmune responses in a murine model of cardiac allograft transplantation. Methods Naïve CBA mice (H2k) underwent transplantation of a C57BL/6 (B6, H2b) heart and were exposed to one of three types of music--opera (La Traviata), classical (Mozart), and New Age (Enya)--or one of six different single sound frequencies, for 7 days. Additionally, we prepared two groups of CBA recipients with tympanic membrane perforation exposed to opera for 7 days and CBA recipients exposed to opera for 7 days before transplantation (pre-treatment). An adoptive transfer study was performed to determine whether regulatory cells were generated in allograft recipients. Immunohistochemical, cell-proliferation, cytokine, and flow cytometry assessments were also performed. Results CBA recipients of a B6 cardiac graft that were exposed to opera music and Mozart had significantly prolonged allograft survival (median survival times [MSTs], 26.5 and 20 days, respectively), whereas those exposed to a single sound frequency (100, 500, 1000, 5000, 10,000, or 20,000 Hz) or Enya did not (MSTs, 7.5, 8, 9, 8, 7.5, 8.5 and 11 days, respectively). Untreated, CBA mice with tympanic membrane perforations and CBA recipients exposed to opera for 7 days before transplantation (pre-treatment) rejected B6 cardiac grafts acutely (MSTs, 7, 8 and 8 days, respectively). Adoptive transfer of whole splenocytes, CD4+ cells, or CD4+CD25+ cells from opera-exposed primary allograft recipients resulted in significantly prolonged allograft survival in naive secondary recipients (MSTs, 36, 68, and > 100 days, respectively). Proliferation of splenocytes, interleukin (IL)-2 and interferon (IFN)-γ production was suppressed in opera-exposed mice, and production of IL-4 and IL-10 from opera-exposed transplant recipients increased compared to that from splenocytes of untreated recipients. Flow cytometry studies showed an increased CD4+CD25+ Forkhead box P3 (Foxp3)+ cell population in splenocytes from those mice. Conclusion Our findings indicate that exposure to opera music, such as La traviata, could affect such aspects of the peripheral immune response as generation of regulatory CD4+CD25+ cells and up-regulation of anti-inflammatory cytokines, resulting in prolonged allograft survival.

Published
2012
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11. A pilot study of operational tolerance with a regulatory T‐cell‐based cell therapy in living donor liver transplantation

Author

Todo, Satoru, primary, Yamashita, Kenichiro, additional, Goto, Ryoichi, additional, Zaitsu, Masaaki, additional, Nagatsu, Akihisa, additional, Oura, Tetsu, additional, Watanabe, Masaaki, additional, Aoyagi, Takeshi, additional, Suzuki, Tomomi, additional, Shimamura, Tsuyoshi, additional, Kamiyama, Toshiya, additional, Sato, Norihiro, additional, Sugita, Junichi, additional, Hatanaka, Kanako, additional, Bashuda, Hisashi, additional, Habu, Sonoko, additional, Demetris, Anthony J., additional, and Okumura, Ko, additional

Published
2016
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15. INDUCTION OF HYPORESPONSIVENESS TO FULLY ALLOGENEIC CARDIAC GRAFTS BY INTRATRACHEAL DELIVERY OF ALLOANTIGEN1

Author

Shirasugi, Nozomu, primary, Ikeda, Yoshifumi, additional, Akiyama, Yoshinobu, additional, Matsumoto, Kenji, additional, Hamano, Kimikazu, additional, Esato, Kensuke, additional, Bashuda, Hisashi, additional, Yagita, Hideo, additional, Okumura, Ko, additional, Takami, Hiroshi, additional, Kodaira, Susumu, additional, and Niimi, Masanori, additional

Published
2001
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25. Danazol induces prolonged survival of fully allogeneic cardiac grafts and maintains the generation of regulatory CD4+ cells in mice.

Author

Uchiyama, Masateru, Jin, Xiangyuan, Zhang, Qi, Hirai, Toshihito, Bashuda, Hisashi, Watanabe, Toshiaki, Amano, Atsushi, and Niimi, Masanori

Subjects
DANAZOL, TREATMENT of endometriosis, FERTILIZATION in vitro, EMBRYO transfer, HOMOGRAFTS, IMMUNE response, ANIMAL models in research
Abstract

Summary Danazol, a derivative of testosterone, is useful for treatment of endometriosis as well as pretreatment for in vitro fertilization and embryo transfer, although its mechanisms of action are unclear. The aim of this study was to investigate the effect of danazol on alloimmune responses in murine heart transplantation. CBA male mice (H2k) underwent transplantation of C57BL/6 male (H2b) hearts and received a single dose of danazol (0.4, 1.2 or 4 mg/kg/day) by intraperitoneal injection on the day of transplantation and for 6 days thereafter. An adoptive transfer study was performed to determine whether regulatory cells were generated. The median survival time (MST) of allografts in danazol-treated (1.2 and 4 mg/kg/day) mice was 28 and 63 days, respectively, compared with 7 days in untreated mice. Moreover, secondary CBA recipients given whole splenocytes or CD4+ cells from primary danazol-treated (4 mg/kg/day) CBA recipients 30 days after transplantation had prolonged allograft survival (MSTs, 29 and 60 days, respectively). Cell proliferation, interleukin (IL)-2 and interferon-γ were suppressed in danazol-treated mice, whereas IL-4 and IL-10 were up-regulated. Moreover, danazol directly suppressed allo-proliferation in a mixed leukocyte culture. Flow cytometry showed an increased CD4+ CD25+ Foxp3+ cell population in splenocytes from danazol-treated mice. Danazol prolongs cardiac allograft survival and generates regulatory CD4+ cells. [ABSTRACT FROM AUTHOR]

Published
2012
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29. An Agonistic Anti-BTLA mAb (3C10) Induced Generation of IL-10-Dependent Regulatory CD4T Cells and Prolongation of Murine Cardiac Allograft

Author

Uchiyama, Masateru, Jin, Xiangyuan, Matsuda, Hironori, Bashuda, Hisashi, Imazuru, Tomohiro, Shimokawa, Tomoki, Yagita, Hideo, and Niimi, Masanori

Abstract

The co-inhibitory receptor B and T lymphocyte attenuator (BTLA) has been implicated in the regulation of autoimmunity and may potentially play an important role in allograft tolerance. This study investigated the effect of an agonistic anti-BTLA mAb (3C10) in the fully major histocompatibility complex–mismatched murine cardiac transplantation.

Published
2014
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31. LACK OF COGNATE HELP BY CD4+T CELLS AND ANERGY OF CD8+T CELLS ARE THE PRINCIPAL MECHANISMS FOR ANTI-LEUKOCYTE FUNCTION-ASSOCIATED ANTIGEN-1/INTERCELLULAR ADHESION MOLECULE-1-INDUCED CARDIAC ALLOGRAFT TOLERANCE

Author

Bashuda, Hisashi, Seino, Kenichiro, Ra, Chisei, Yagita, Hideo, and Okumura1, Ko

Abstract

Combined treatment with anti-leukocyte function-associated antigen-1 (LFA-1) and anti-intercellular adhesion molecule-1 (ICAM-1) monoclonal antibodies leads to allograft tolerance in murine cardiac transplantation. In the present study, we analyzed the mechanisms for this tolerance induction. In the tolerant mice, proliferative response of splenic T cells against donor-type cardiac myocytes and of CD8+T cells against donor-type alloantigens was impaired as compared with responses in naive or rejected mice, but was completely restored with exogenous interleukin 2. This suggests that class I-restricted CD8+T cells of tolerant mice were rendered anergic against donor-type alloantigens in the periphery. In contrast, proliferative response of CD4+T cells against donor-type alloantigens in vitro was comparable between tolerant and naive mice. When heart and skin grafts from the same donor (BALB/c [H2d]) were simultaneously transplanted to C3H mice (H2k), both were rejected within 29 days, even though the mice were similarly treated with anti-LFA-1 and anti-ICAM-1 monoclonal antibodies. In contrast, when heart graft from BALB/c and skin graft from third-party donor (C57BL/6 [H2b]) were simultaneously transplanted to C3H mice under the same condition, the heart graft was accepted indefinitely and the skin graft was rejected. These findings suggest that the peripheral tolerance against cardiac allografts could be induced by selective inactivation of alloreactive CD8+T cells resulting from the lack of cognate help by CD4+T cells.

Published
1997

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