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308 results on '"Basdra, Efthimia"'

4. Characterization of the First Secreted Sorting Nexin Identified in the Leishmania Protists.

Author

Tziouvara, Olympia, Petsana, Marina, Kourounis, Drosos, Papadaki, Amalia, Basdra, Efthimia, Braliou, Georgia G., and Boleti, Haralabia

Subjects
GREEN fluorescent protein, PROTISTA, BINDING site assay, EUKARYOTIC cells, TERTIARY structure, COATED vesicles
Abstract

Proteins of the sorting nexin (SNX) family present a modular structural architecture with a phox homology (PX) phosphoinositide (PI)-binding domain and additional PX structural domains, conferring to them a wide variety of vital eukaryotic cell's functions, from signal transduction to membrane deformation and cargo binding. Although SNXs are well studied in human and yeasts, they are poorly investigated in protists. Herein, is presented the characterization of the first SNX identified in Leishmania protozoan parasites encoded by the LdBPK_352470 gene. In silico secondary and tertiary structure prediction revealed a PX domain on the N-terminal half and a Bin/amphiphysin/Rvs (BAR) domain on the C-terminal half of this protein, with these features classifying it in the SNX-BAR subfamily of SNXs. We named the LdBPK_352470.1 gene product LdSNXi, as it is the first SNX identified in Leishmania (L.) donovani. Its expression was confirmed in L. donovani promastigotes under different cell cycle phases, and it was shown to be secreted in the extracellular medium. Using an in vitro lipid binding assay, it was demonstrated that recombinant (r) LdSNXi (rGST-LdSNXi) tagged with glutathione-S-transferase (GST) binds to the PtdIns3P and PtdIns4P PIs. Using a specific a-LdSNXi antibody and immunofluorescence confocal microscopy, the intracellular localization of endogenous LdSNXi was analyzed in L. donovani promastigotes and axenic amastigotes. Additionally, rLdSNXi tagged with enhanced green fluorescent protein (rLdSNXi-EGFP) was heterologously expressed in transfected HeLa cells and its localization was examined. All observed localizations suggest functions compatible with the postulated SNX identity of LdSNXi. Sequence, structure, and evolutionary analysis revealed high homology between LdSNXi and the human SNX2, while the investigation of protein–protein interactions based on STRING (v.11.5) predicted putative molecular partners of LdSNXi in Leishmania. [ABSTRACT FROM AUTHOR]

Published
2024
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11. How to improve translatability and clinical relevance of preclinical studies in rheumatoid arthritis.

Author

Kalliolias, George D., Basdra, Efthimia K., and Papavassiliou, Athanasios G.

Subjects
*RHEUMATOID arthritis, *ABATACEPT, *EXPERIMENTAL arthritis, *TUMOR necrosis factors
Abstract

This article discusses the limitations of current preclinical studies in rheumatoid arthritis (RA) and proposes ways to improve their translatability and clinical relevance. The use of animal models of arthritis and conventional in vitro systems is common in RA research, but they have several weaknesses that compromise their effectiveness. Animal models only mimic certain aspects of RA and have poor translatability to human diseases. Conventional in vitro systems are too simplistic and do not accurately represent the complex pathogenesis of RA. The article suggests using more advanced ex vivo systems, such as 3D synovial organoid platforms, to better model the disease and identify disease-relevant pathogenic subsets. These advancements could lead to better preclinical validation of potential therapeutic treatments for RA. [Extracted from the article]

Published
2024
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20. Polycystin‐1 regulates cell proliferation and migration through AKT/mTORC2 pathway in a human craniosynostosis cell model

Author

Katsianou, Maria A., primary, Papavassiliou, Kostas A., additional, Gargalionis, Antonios N., additional, Agrogiannis, George, additional, Korkolopoulou, Penelope, additional, Panagopoulos, Dimitrios, additional, Themistocleous, Marios S., additional, Piperi, Christina, additional, Basdra, Efthimia K., additional, and Papavassiliou, Athanasios G., additional

Published
2022
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26. Polycystin-1 and polycystin-2 are involved in the acquisition of aggressive phenotypes in colorectal cancer

Author

Gargalionis, Antonios N., Korkolopoulou, Penelope, Farmaki, Elena, Piperi, Christina, Dalagiorgou, Georgia, Adamopoulos, Christos, Levidou, Georgia, Saetta, Angelica, Fragkou, Paraskevi, Tsioli, Panagiota, Kiaris, Hippokratis, Zizi-Serbetzoglou, Adamantia, Karavokyros, Ioannis, Papavassiliou, Kostas A., Tsavaris, Nikolaos, Patsouris, Efstratios, Basdra, Efthimia K., and Papavassiliou, Athanasios G.

Published
2015
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34. Polycystin‐1 modulates RUNX2 activation and osteocalcin gene expression via ERK signalling in a human craniosynostosis cell model

Author

Katsianou, Maira, primary, Papavassiliou, Kostas A., additional, Zoi, Ilianna, additional, Gargalionis, Antonios N., additional, Panagopoulos, Dimitrios, additional, Themistocleous, Marios S., additional, Piperi, Christina, additional, Papavassiliou, Athanasios G., additional, and Basdra, Efthimia K., additional

Published
2021
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42. Polycystins in Colorectal Cancer

Author

Gargalionis, Antonios N. Basdra, Efthimia K. Papavassiliou, Athanasios G.

Subjects
urogenital system, embryonic structures, urologic and male genital diseases, female genital diseases and pregnancy complications
Abstract

Cell and extracellular matrix (ECM) biomechanics emerge as a distinct feature during the development and progression of colorectal cancer (CRC). Polycystins are core mechanosensitive protein molecules that mediate mechanotransduction in a variety of epithelial cells. Polycystin-1 (PC1) and polycystin-2 (PC2) are engaged in signal transduction mechanisms and during alterations in calcium influx, which regulate cellular functions such as proliferation, differentiation, orientation, and migration in cancer cells. Recent findings implicate polycystins in the deregulation of such functions and the formation of CRC invasive phenotypes. Polycystins participate in all aspects of the cell's biomechanical network, from the perception of extracellular mechanical cues to focal adhesion protein and nuclear transcriptional complexes. Therefore, polycystins could be employed as novel biomarkers and putative targets of selective treatment in CRC.

Published
2019

46. The role of transient receptor potential polycystin channels in bone diseases

Author

Katsianou, Maria A. Skondra, Foteini G. Gargalionis, Antonios N. and Piperi, Christina Basdra, Efthimia K.

Abstract

Transient receptor potential (TRP) channels are cation channels which act as molecular sensors that enable cells to detect and respond to a plethora of mechanical and environmental cues. TRPs are involved in various physiological processes, such as mechanosensation, non-inception and thermosensation, while mutations in genes encoding them can lead to pathological conditions, called “channelopathies”. The subfamily of transient receptor potential polycystins (TRPPs), Polycystin 1 (PC1, TRPP1) and Polycystin 2 (PC2, TRPP2), act as mechanoreceptors, sensing external mechanical forces, including strain, stretch and fluid shear stress, triggering a cascade of signaling pathways involved in osteoblastogenesis and ultimately bone formation. Both in vitro studies and research on animal models have already identified their implications in bone homeostasis. However, uncertainty veiling the role of polycystins (PCs) in bone disease urges studies to elucidate further their role in this field. Mutations in TRPPs have been related to autosomal polycystic kidney disease (ADKPD) and research groups try to identify their role beyond their well-established contribution in kidney disease. Such an elucidation would be beneficial for identifying signaling pathways where polycystins are involved in bone diseases related to exertion of mechanical forces such as osteoporosis, osteopenia and craniosynostosis. A better understanding of the implications of TRPPs in bone diseases would possibly lay the cornerstone for effective therapeutic schemes.

Published
2018

47. Molecular mechanisms of mechanotransduction in psoriasis

Author

Malakou, Lina S. Gargalionis, Antonios N. Piperi, Christina and Papadavid, Evangelia Papavassiliou, Athanasios G. Basdra, Efthimia K.

Abstract

Psoriasis is an immune disease of the skin that frequently develops upon triggering events of mechanical nature and leads to increased proliferation and damaged differentiation of keratinocytes of the epidermis. Mechanical forces are mediated through mechanotransduction, which is the process that translates physical cues into biochemical signaling networks. Latest updates underline the role of mechanotransduction during the acquisition of aberrant properties by the keratinocytes of the skin, therefore implying a potential contribution that promotes psoriasis pathogenesis. The present review discusses the mechano-induced signaling pathways and individual molecules that become activated in psoriasis and in keratinocytes, along with mechano-based putative treatment strategies. We also suggest emerging mechanosensitive molecules for further investigation with potential diagnostic and therapeutic utility in psoriasis.

Published
2018

50. Gene-Specific Intron Retention Serves as Molecular Signature that Distinguishes Melanoma from Non-Melanoma Cancer Cells in Greek Patients

Author

Giannopoulou, Aikaterini, primary, Konstantakou, Eumorphia, additional, Velentzas, Athanassios, additional, Avgeris, Socratis, additional, Avgeris, Margaritis, additional, Papandreou, Nikos, additional, Zoi, Ilianna, additional, Filippa, Vicky, additional, Katarachia, Stamatia, additional, Lampidonis, Antonis, additional, Prombona, Anastasia, additional, Syntichaki, Popi, additional, Piperi, Christina, additional, Basdra, Efthimia, additional, Iconomidou, Vassiliki, additional, Papadavid, Evangelia, additional, Anastasiadou, Ema, additional, Papassideri, Issidora, additional, Papavassiliou, Athanasios, additional, Voutsinas, Gerassimos, additional, Scorilas, Andreas, additional, and Stravopodis, Dimitrios, additional

Published
2019
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