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14 results on '"Basatvat, Shaghayegh"'

4. Recommendations for intervertebral disc notochordal cell investigation: From isolation to characterization

Author

Williams, Rebecca J., primary, Laagland, Lisanne T., additional, Bach, Frances C., additional, Ward, Lizzy, additional, Chan, Wilson, additional, Tam, Vivian, additional, Medzikovic, Adel, additional, Basatvat, Shaghayegh, additional, Paillat, Lily, additional, Vedrenne, Nicolas, additional, Snuggs, Joseph W., additional, Poramba‐Liyanage, Deepani W., additional, Hoyland, Judith A., additional, Chan, Danny, additional, Camus, Anne, additional, Richardson, Stephen M., additional, Tryfonidou, Marianna A., additional, and Le Maitre, Christine L., additional

Published
2023
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5. Harmonization and standardization of nucleus pulposus cell extraction and culture methods

Author

ORT Research, Regenerative Medicine and Stem Cells, Basatvat, Shaghayegh, Bach, Frances C, Barcellona, Marcos N, Binch, Abbie L, Buckley, Conor T, Bueno, Brian, Chahine, Nadeen O, Chee, Ana, Creemers, Laura B, Dudli, Stefan, Fearing, Bailey, Ferguson, Stephen J, Gansau, Jennifer, Gantenbein, Benjamin, Gawri, Rahul, Glaeser, Juliane D, Grad, Sibylle, Guerrero, Julien, Haglund, Lisbet, Hernandez, Paula A, Hoyland, Judith A, Huang, Charles, Iatridis, James C, Illien-Junger, Svenja, Jing, Liufang, Kraus, Petra, Laagland, Lisanne T, Lang, Gernot, Leung, Victor, Li, Zhen, Lufkin, Thomas, van Maanen, Josette C, McDonnell, Emily E, Panebianco, Chris J, Presciutti, Steven M, Rao, Sanjna, Richardson, Stephen M, Romereim, Sarah, Schmitz, Tara C, Schol, Jordy, Setton, Lori, Sheyn, Dmitriy, Snuggs, Joseph W, Sun, Y, Tan, Xiaohong, Tryfonidou, Marianna A, Vo, Nam, Wang, Dong, Williams, Brandon, Williams, Rebecca, Yoon, S Tim, Le Maitre, Christine L, ORT Research, Regenerative Medicine and Stem Cells, Basatvat, Shaghayegh, Bach, Frances C, Barcellona, Marcos N, Binch, Abbie L, Buckley, Conor T, Bueno, Brian, Chahine, Nadeen O, Chee, Ana, Creemers, Laura B, Dudli, Stefan, Fearing, Bailey, Ferguson, Stephen J, Gansau, Jennifer, Gantenbein, Benjamin, Gawri, Rahul, Glaeser, Juliane D, Grad, Sibylle, Guerrero, Julien, Haglund, Lisbet, Hernandez, Paula A, Hoyland, Judith A, Huang, Charles, Iatridis, James C, Illien-Junger, Svenja, Jing, Liufang, Kraus, Petra, Laagland, Lisanne T, Lang, Gernot, Leung, Victor, Li, Zhen, Lufkin, Thomas, van Maanen, Josette C, McDonnell, Emily E, Panebianco, Chris J, Presciutti, Steven M, Rao, Sanjna, Richardson, Stephen M, Romereim, Sarah, Schmitz, Tara C, Schol, Jordy, Setton, Lori, Sheyn, Dmitriy, Snuggs, Joseph W, Sun, Y, Tan, Xiaohong, Tryfonidou, Marianna A, Vo, Nam, Wang, Dong, Williams, Brandon, Williams, Rebecca, Yoon, S Tim, and Le Maitre, Christine L

Published
2023

6. Harmonization and standardization of nucleus pulposus cell extraction and culture methods

Author

Basatvat, Shaghayegh; https://orcid.org/0000-0001-6201-4588, Bach, Frances C; https://orcid.org/0000-0002-4481-0051, Barcellona, Marcos N; https://orcid.org/0000-0003-0694-3555, Binch, Abbie L, Buckley, Conor T; https://orcid.org/0000-0001-7452-4534, Bueno, Brian; https://orcid.org/0000-0003-1952-6638, Chahine, Nadeen O; https://orcid.org/0000-0002-0478-6042, Chee, Ana; https://orcid.org/0000-0002-7977-2737, Creemers, Laura B; https://orcid.org/0000-0002-1585-3052, Dudli, Stefan; https://orcid.org/0000-0001-7351-713X, Fearing, Bailey; https://orcid.org/0000-0002-9784-4693, Ferguson, Stephen J; https://orcid.org/0000-0003-0650-5889, Gansau, Jennifer; https://orcid.org/0000-0002-1951-2933, Gantenbein, Benjamin; https://orcid.org/0000-0002-9005-0655, Gawri, Rahul; https://orcid.org/0000-0001-9415-1739, Glaeser, Juliane D, Grad, Sibylle; https://orcid.org/0000-0001-9552-3653, Guerrero, Julien; https://orcid.org/0000-0003-4130-4230, Haglund, Lisbet; https://orcid.org/0000-0002-1288-2149, Hernandez, Paula A; https://orcid.org/0000-0002-8785-7351, Hoyland, Judith A; https://orcid.org/0000-0003-4876-5208, Huang, Charles; https://orcid.org/0000-0002-8848-258X, Iatridis, James C; https://orcid.org/0000-0002-2186-0590, Illien-Junger, Svenja; https://orcid.org/0000-0001-9895-0693, Jing, Liufang, Kraus, Petra; https://orcid.org/0000-0002-6716-5550, Laagland, Lisanne T; https://orcid.org/0000-0002-4456-6167, Lang, Gernot, Leung, Victor; https://orcid.org/0000-0002-3406-7225, Li, Zhen; https://orcid.org/0000-0002-9754-6389, et al, Basatvat, Shaghayegh; https://orcid.org/0000-0001-6201-4588, Bach, Frances C; https://orcid.org/0000-0002-4481-0051, Barcellona, Marcos N; https://orcid.org/0000-0003-0694-3555, Binch, Abbie L, Buckley, Conor T; https://orcid.org/0000-0001-7452-4534, Bueno, Brian; https://orcid.org/0000-0003-1952-6638, Chahine, Nadeen O; https://orcid.org/0000-0002-0478-6042, Chee, Ana; https://orcid.org/0000-0002-7977-2737, Creemers, Laura B; https://orcid.org/0000-0002-1585-3052, Dudli, Stefan; https://orcid.org/0000-0001-7351-713X, Fearing, Bailey; https://orcid.org/0000-0002-9784-4693, Ferguson, Stephen J; https://orcid.org/0000-0003-0650-5889, Gansau, Jennifer; https://orcid.org/0000-0002-1951-2933, Gantenbein, Benjamin; https://orcid.org/0000-0002-9005-0655, Gawri, Rahul; https://orcid.org/0000-0001-9415-1739, Glaeser, Juliane D, Grad, Sibylle; https://orcid.org/0000-0001-9552-3653, Guerrero, Julien; https://orcid.org/0000-0003-4130-4230, Haglund, Lisbet; https://orcid.org/0000-0002-1288-2149, Hernandez, Paula A; https://orcid.org/0000-0002-8785-7351, Hoyland, Judith A; https://orcid.org/0000-0003-4876-5208, Huang, Charles; https://orcid.org/0000-0002-8848-258X, Iatridis, James C; https://orcid.org/0000-0002-2186-0590, Illien-Junger, Svenja; https://orcid.org/0000-0001-9895-0693, Jing, Liufang, Kraus, Petra; https://orcid.org/0000-0002-6716-5550, Laagland, Lisanne T; https://orcid.org/0000-0002-4456-6167, Lang, Gernot, Leung, Victor; https://orcid.org/0000-0002-3406-7225, Li, Zhen; https://orcid.org/0000-0002-9754-6389, and et al

Abstract

BACKGROUND: In vitro studies using nucleus pulposus (NP) cells are commonly used to investigate disc cell biology and pathogenesis, or to aid in the development of new therapies. However, lab-to-lab variability jeopardizes the much-needed progress in the field. Here, an international group of spine scientists collaborated to standardize extraction and expansion techniques for NP cells to reduce variability, improve comparability between labs and improve utilization of funding and resources. METHODS: The most commonly applied methods for NP cell extraction, expansion, and re-differentiation were identified using a questionnaire to research groups worldwide. NP cell extraction methods from rat, rabbit, pig, dog, cow, and human NP tissue were experimentally assessed. Expansion and re-differentiation media and techniques were also investigated. RESULTS: Recommended protocols are provided for extraction, expansion, and re-differentiation of NP cells from common species utilized for NP cell culture. CONCLUSIONS: This international, multilab and multispecies study identified cell extraction methods for greater cell yield and fewer gene expression changes by applying species-specific pronase usage, 60-100 U/ml collagenase for shorter durations. Recommendations for NP cell expansion, passage number, and many factors driving successful cell culture in different species are also addressed to support harmonization, rigor, and cross-lab comparisons on NP cells worldwide.

Published
2023

7. Harmonization and standardization of nucleus pulposus cell extraction and culture methods

Author

Basatvat, Shaghayegh, Bach, Frances C, Barcellona, Marcos N, Binch, Abbie L, Buckley, Conor T, Bueno, Brian, Chahine, Nadeen O, Chee, Ana, Creemers, Laura B, Dudli, Stefan, Fearing, Bailey, Ferguson, Stephen J, Gansau, Jennifer, Gantenbein, Benjamin, Gawri, Rahul, Glaeser, Juliane D, Grad, Sibylle, Guerrero, Julien, Haglund, Lisbet, Hernandez, Paula A, Hoyland, Judith A, Huang, Charles, Iatridis, James C, Illien-Junger, Svenja, Jing, Liufang, Kraus, Petra, Laagland, Lisanne T, Lang, Gernot, Leung, Victor, Li, Zhen, Lufkin, Thomas, van Maanen, Josette C, McDonnell, Emily E, Panebianco, Chris J, Presciutti, Steven M, Rao, Sanjna, Richardson, Stephen M, Romereim, Sarah, Schmitz, Tara C, Schol, Jordy, Setton, Lori, Sheyn, Dmitriy, Snuggs, Joseph W, Sun, Y, Tan, Xiaohong, Tryfonidou, Marianna A, Vo, Nam, Wang, Dong, Williams, Brandon, Williams, Rebecca, Yoon, S Tim, and Le Maitre, Christine L

Subjects
intervertebral, standardization, culture, harmonization, in vitro, nucleus pulposus, Orthopedics and Sports Medicine, 610 Medicine & health, 610 Medizin und Gesundheit
Abstract

Background: In vitro studies using nucleus pulposus (NP) cells are commonly used to investigate disc cell biology and pathogenesis, or to aid in the development of new therapies. However, lab-to-lab variability jeopardizes the much-needed progress in the field. Here, an international group of spine scientists collaborated to standardize extraction and expansion techniques for NP cells to reduce variability, improve comparability between labs and improve utilization of funding and resources.Methods: The most commonly applied methods for NP cell extraction, expansion, and re-differentiation were identified using a questionnaire to research groups worldwide. NP cell extraction methods from rat, rabbit, pig, dog, cow, and human NP tissue were experimentally assessed. Expansion and re-differentiation media and techniques were also investigated.Results: Recommended protocols are provided for extraction, expansion, and re-differentiation of NP cells from common species utilized for NP cell culture.Conclusions: This international, multilab and multispecies study identified cell extraction methods for greater cell yield and fewer gene expression changes by applying species-specific pronase usage, 60-100 U/ml collagenase for shorter durations. Recommendations for NP cell expansion, passage number, and many factors driving successful cell culture in different species are also addressed to support harmonization, rigor, and cross-lab comparisons on NP cells worldwide., JOR Spine, 6 (1), ISSN:2572-1143

Published
2023

9. Understanding the dynamics of Toll-like Receptor 5 response to flagellin and its regulation by estradiol

Author

Caballero, Ignacio, primary, Boyd, James, additional, Almiñana, Carmen, additional, Sánchez-López, Javier A., additional, Basatvat, Shaghayegh, additional, Montazeri, Mehrnaz, additional, Maslehat Lay, Nasim, additional, Elliott, Sarah, additional, Spiller, David G., additional, White, Michael R. H., additional, and Fazeli, Alireza, additional

Published
2017
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10. Development of a functional in vitro cell system to test the role of Tribbles-2 in embryo implantation

Author

Basatvat, Shaghayegh, Caballero, Ignacio, Montazeri, Mehrnaz, Sanchez-Lopez, Javier Arturo, Maslehat Lay, N., Carter, D., Elliott, S., Kiss-Toth, E., Fazeli, Alireza, Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT), Academic Unit of Reproductive and Developmental Medicine, University of Sheffield [Sheffield], Biochemical Society. GBR., Institut National de la Recherche Agronomique (INRA)-Université de Tours, and ProdInra, Migration

Subjects
[SDV.BA] Life Sciences [q-bio]/Animal biology, [SDV.BA]Life Sciences [q-bio]/Animal biology, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2014

13. Tribbles in inflammation

Author

Johnston, Jessica, primary, Basatvat, Shaghayegh, additional, Ilyas, Zabran, additional, Francis, Sheila, additional, and Kiss-Toth, Endre, additional

Published
2015
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