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In the present investigation metal (II) complexes containing binuclear Co (II), mono nuclear Ni (II), and Cu (II) with ligand 2E-N-phenyl-2-[([1,3]thiazolo[3,2-a]benzimidazol-2yl) methylidene]hydrazine-1-carbothioamide moiety were synthesized in ethanolic medium, recrystallized and investigated for their biological properties. The carbothioamide was derived from the reaction of benzo [4, 5] imidazo [2, 1-b] thiazole-2-carbaldehyde and 4-phenyl-3-thiosemicarbazide at reflux temperature. The newly formed compounds were characterized by 1H-nuclear magnetic resonance (1H-NMR), 13C-nuclear magnetic resonance (13C-NMR), Fourier transformed infrared spectroscopy (FT-IR), ESR spectroscopic techniques and elemental analysis. Ligand containing “O” and “S” donor atoms participated in coordination with metal (II) complexes and are confirmed by FT-IR, electronic absorption spectra, 1H-NMR data. DNA binding properties of these metal complexes were investigated by ESR, magnetic moments and electronic absorption spectroscopy. Antibacterial studies, DNA binding constants of the complexes indicated intercalative binding mode involving strong aromatic chromophoric groups and base pairs of DNA. All the complexes (2a, 2b and 2c) showed intercalative binding with DNA and DNA cleavage was studied in presence and absence of H2O2 which was observed for copper (II) complex. Antibacterial study results showed that cobalt complex inhibit the growth of Staphylococcus epidermis, Bacillus subtilis, Pseudomonas aeruginosa and Escherichia coli with MIC values of 7.98 µg/mL, 8.12 µg/mL, 7.34 µg/mL, and 7.56 µg/mL respectively. Metal (II) complexes exhibit higher antibacterial activity than the parent ligand.

Continuous monitoring of glucose and sugar sensing plays a vital role in diabetes control. The drawbacks of the present enzyme-based sugar sensors have encouraged the investigation into alternate approaches to design new sensors. The popularity of fluorescence sensors is due to their ability to bind reversibly to compounds containing diol. In this study we investigated the binding ability of phenyl boronic acid P1 for monosaccharides and disaccharides (sugars) in aqueous medium at physiological pH 7.4 using steady-state fluorescence and absorbance. P1 fluorescence was quenched due to formation of esters with sugars. Absorbance and fluorescence measurements led to results that indicated that the sugars studied could be ordered in terms of their affinity to P1, as stated: sucrose > lactose > galactose > xylose > ribose > arabinose. In each case, the slope of modified Stern-Volmer plots was nearly 1, indicating the presence of only a single binding site in boronic acids for sugars. Docking studies were carried out using Schrodinger Maestro v.11.2 software. The binding affinity of phenyl boronic acid P1 with periplasmic protein (PDB ID 2IPM and 2IPL) was estimated using GlideScore.