24 results on '"Barranquero, Jose"'
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2. Biological role of EPS from Pseudomonas syringae pv. syringae UMAF0158 extracellular matrix, focusing on a Psl-like polysaccharide
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Heredia-Ponce, Zaira, Gutiérrez-Barranquero, Jose Antonio, Purtschert-Montenegro, Gabriela, Eberl, Leo, Cazorla, Francisco M., and de Vicente, Antonio
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- 2020
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3. Multimodal optimization: An effective framework for model calibration
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Chica, Manuel, Barranquero, José, Kajdanowicz, Tomasz, Damas, Sergio, and Cordón, Óscar
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- 2017
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4. Quantification-oriented learning based on reliable classifiers
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Barranquero, Jose, Díez, Jorge, and José del Coz, Juan
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- 2015
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5. Interplay between rhizospheric Pseudomonas chlororaphis strains lays the basis for beneficial bacterial consortia
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Villar-Moreno, Rafael, primary, Tienda, Sandra, additional, Gutiérrez-Barranquero, Jose A., additional, Carrión, Víctor J., additional, de Vicente, Antonio, additional, Cazorla, Francisco M., additional, and Arrebola, Eva, additional
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- 2022
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6. Dependent binary relevance models for multi-label classification
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Montañes, Elena, Senge, Robin, Barranquero, Jose, Ramón Quevedo, José, José del Coz, Juan, and Hüllermeier, Eyke
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- 2014
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7. Enhancing directed binary trees for multi-class classification
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Montañés, Elena, Barranquero, Jose, Díez, Jorge, and del Coz, Juan José
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- 2013
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8. On the study of nearest neighbor algorithms for prevalence estimation in binary problems
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Barranquero, Jose, González, Pablo, Díez, Jorge, and del Coz, Juan José
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- 2013
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9. Mining Microbial Signals for Enhanced Biodiscovery of Secondary Metabolites
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Reen, F. Jerry, primary, Gutiérrez-Barranquero, Jose A., additional, and O’Gara, Fergal, additional
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- 2016
- Full Text
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10. Sclerotization as a long-term preservation method for Rosellinia necatrix strains
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Gutiérrez-Barranquero, Jose A., Pliego, Clara, Bonilla, Nuria, Calderón, Claudia E., Pérez-García, Alejandro, de Vicente, Antonio, and Cazorla, Francisco M.
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- 2012
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11. Biological role of EPS from Pseudomonas syringae pv. syringae UMAF0158 extracellular matrix, focusing on a Psl-like polysaccharide
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Heredia-Ponce, Zaira; https://orcid.org/0000-0002-8707-6168, Gutiérrez-Barranquero, Jose Antonio; https://orcid.org/0000-0003-1810-699X, Purtschert-Montenegro, Gabriela, Eberl, Leo; https://orcid.org/0000-0002-7241-0864, Cazorla, Francisco M, de Vicente, Antonio; https://orcid.org/0000-0003-2716-9861, Heredia-Ponce, Zaira; https://orcid.org/0000-0002-8707-6168, Gutiérrez-Barranquero, Jose Antonio; https://orcid.org/0000-0003-1810-699X, Purtschert-Montenegro, Gabriela, Eberl, Leo; https://orcid.org/0000-0002-7241-0864, Cazorla, Francisco M, and de Vicente, Antonio; https://orcid.org/0000-0003-2716-9861
- Abstract
Pseudomonas syringae is a phytopathogenic model bacterium that is used worldwide to study plant–bacteria interactions and biofilm formation in association with a plant host. Within this species, the syringae pathovar is the most studied due to its wide host range, affecting both, woody and herbaceous plants. In particular, Pseudomonas syringae pv. syringae (Pss) has been previously described as the causal agent of bacterial apical necrosis on mango trees. Pss exhibits major epiphytic traits and virulence factors that improve its epiphytic survival and pathogenicity in mango trees. The cellulose exopolysaccharide has been described as a key component in the development of the biofilm lifestyle of the P. syringae pv. syringae UMAF0158 strain (PssUMAF0158). PssUMAF0158 contains two additional genomic regions that putatively encode for exopolysaccharides such as alginate and a Psl-like polysaccharide. To date, the Psl polysaccharide has only been studied in Pseudomonas aeruginosa, in which it plays an important role during biofilm development. However, its function in plant-associated bacteria is still unknown. To understand how these exopolysaccharides contribute to the biofilm matrix of PssUMAF0158, knockout mutants of genes encoding these putative exopolysaccharides were constructed. Flow-cell chamber experiments revealed that cellulose and the Psl-like polysaccharide constitute a basic scaffold for biofilm architecture in this bacterium. Curiously, the Psl-like polysaccharide of PssUMAF0158 plays a role in virulence similar to what has been described for cellulose. Finally, the impaired swarming motility of the Psl-like exopolysaccharide mutant suggests that this exopolysaccharide may play a role in the motility of PssUMAF0158 over the mango plant surface.
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- 2020
12. Captación, abuso y control psicológico. El caso ejemplar de Patricia Aguilar
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Cuevas-Barranquero, Jose Miguel
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grooming ,exit counselling ,recruitment ,cults ,coercive persuasion ,psychological abuse ,control ,Patricia Aguilar - Abstract
Comunicación presentada en el marco del Coloquio Europeo de la Federación Europea de Centros de Información e Investigación, como caso ilustrativo de las estrategias de captación y proselitismo de las sectas empleando las nuevas tecnologías de la información y las redes sociales. Patricia Aguilar was a minor from Elche (a town in the South East of Spain), 16 years old, when she had her first contact through the internet with Felix Steven Manrique, who was based in Lima, Peru. She visits a Facebook group on esotericism and paranormal life. It is there that someone, who initially claims to be a spiritual guide, offers her to interpret some dreams she had been having which made her restless. This is where the first contacts begin. Felix Steven used multiple identities on the net. Just like in his real life where, among other attributes, he claimed to have an NGO and to be a sociologist, on the net he used multiple nicks, with different identities and with constant reinventions of his character. Gradually he introduced her into the group's doctrine, making her believe that she was with the reincarnation of a great Master, the self-proclaimed "Prince Gurdjieff", who belonged to an important white lodge, which had direct contact with great and elevated Masters. The dismantling of the group came about through the joint collaboration of multiple factors. Patricia has been missing for many months. Many, including some family members, came to fear that the worst of the endings could have happened. The happy ending of this exemplary case cannot make us forget the process: it was a road full of obstacles, difficulties, negatives, questions, incomprehension, crying, fear, uncertainty, ... Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.
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- 2019
13. Quorum Sensing Signaling Alters Virulence Potential and Population Dynamics in Complex Microbiome-Host Interactomes
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Reen, F. Jerry, Gutierrez-Barranquero, Jose A., McCarthy, Ronan R., Woods, David F., Scarciglia, Sara, Adams, Claire, Nielsen, Kristian Fog, Gram, Lone, O'Gara, Fergal, Reen, F. Jerry, Gutierrez-Barranquero, Jose A., McCarthy, Ronan R., Woods, David F., Scarciglia, Sara, Adams, Claire, Nielsen, Kristian Fog, Gram, Lone, and O'Gara, Fergal
- Abstract
Despite the discovery of the first N-acyl homoserine lactone (AHL) based quorum sensing (QS) in the marine environment, relatively little is known about the abundance, nature and diversity of AHL QS systems in this diverse ecosystem. Establishing the prevalence and diversity of AHL QS systems and how they may influence population dynamics within the marine ecosystem, may give a greater insight into the evolution of AHLs as signaling molecules in this important and largely unexplored niche. Microbiome profiling of Stelletta normani and BD1268 sponge samples identified several potential QS active genera. Subsequent biosensor-based screening of a library of 650 marine sponge bacterial isolates identified 10 isolates that could activate at least one of three AHL biosensor strains. Each was further validated and profiled by Ultra-High Performance Liquid Chromatography Mass Spectrometry, with AHLs being detected in 8 out of 10 isolate extracts. Co-culture of QS active isolates with S. normani marine sponge samples led to the isolation of genera such as Pseudomonas and Paenibacillus, both of which were low abundance in the S. normani microbiome. Surprisingly however, addition of AHLs to isolates harvested following co-culture did not measurably affect either growth or biofilm of these strains. Addition of supernatants from QS active strains did however impact significantly on biofilm formation of the marine Bacillus sp. CH8a sporeforming strain suggesting a role for QS systems in moderating the microbe-microbe interaction in marine sponges. Genome sequencing and phylogenetic analysis of a QS positive Psychrobacter isolate identified several QS associated systems, although no classical QS synthase gene was identified. The stark contrast between the biodiverse sponge microbiome and the relatively limited diversity that was observed on standard culture media, even in the presence of QS active compounds, serves to underscore the extent of diversity that remains to be brought i
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- 2019
14. Tratamiento y asesoramiento de las dependencias grupales desde una institución pública. Experiencias Clínicas, abordaje psicoterapéutico y limitaciones
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Cuevas-Barranquero, Jose Miguel
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Familias ,Manipulación ,Dependencia ,Tratamiento ,Sectas ,Abuso psicológico grupal ,Asesoramiento ,Mediación ,Dinámica de grupos ,Persuasión coercitiva - Abstract
Se describe el abordaje psicoterapéutico desde un programa público municipal en la atención a víctimas de sectas destructivas y grupos manipulativos Aproximadamente el 1 % de la población española, unas 500.000 personas, podrían estar afectadas en la actualidad, de forma directa, por problemas de sectarismo, en las más de 300 sectas destructivas que operan en nuestro país. Las consecuencias de la afiliación a estos grupos conllevan múltiples consecuencias personales, familiares y sociales. En la Costa del Sol, en Marbella, disponemos del único servicio público que trabaja con víctimas sectarias, dentro del marco de las socioadicciones. Se describe, de forma práctica, los objetivos, las actividades preventivas y las intervenciones que se desarrollan, haciéndose especial hincapié en la práctica clínica, los programas terapéuticos disponibles y las peculiaridades en la intervención. También se repasarán algunas actividades consideradas de riesgo en el ámbito sectario, como la utilización de pseudoterapias o la irrupción de la magia en distintos contextos sociales. Los principales programas que se describen : - Programa de apoyo y asesoramiento a familiares de las víctimas - Programa Asesoramiento en el egreso o exit counselling, que incluye diferentes estrategias informativas, de debate, estrategias motivacionales y, si es posible, aislamiento temporal de las fuentes abusivas, que permitan cuestionar la posible pertenencia a un grupo manipulativo. - Programa de dependencia grupal: donde se atenderán las problemáticas psicosociales de las víctimas desvinculadas de grupos manipulativos, conscientes de su pasado sectario Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.
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- 2018
15. Dharma Tradición, historia de una tiranía
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Cuevas-Barranquero, Jose Miguel
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Coerción ,Sectas - Congresos ,Maltrato ,Criminalidad ,Delitos ,Grupos manipulativos ,Abuso psicológico ,Religión ,Abusos sexuales ,Persuasión coercitiva - Abstract
1. Introducción 2. Etapas del grupo; distintas vías de captación y proselitismo 3. Algunas creencias del grupo 4. El papel de la mujer en Dharma Tradición 5. El cine y la música en el grupo 6. Paco y "sus amigos" 7. Persuasión coercitiva 8. Causas judiciales En la actualidad no existe algo parecido a un registro oficial de “sectas”. Incluso el término "secta" y sus criterios está abiertos a críticas, lo que dificulta su detección y cuantificar su número. Además, algunos de estos grupos son muy reducidos y no constan en un registro oficial como asociación (cultural, religiosa, humanitaria…), ni como entidad mercantil. A pesar de ello hay consenso en cuanto a que en la Costa del Sol hay un gran número de sectas coercitivas. Este artículo en concreto se dedica a una de esas pequeñas sectas coercitivas: Dharma Tradición, asociación “religiosa” que tuvo distintas caras y un líder indiscutible. Abordando la historia y particularidades de este grupo en concreto, se describe cómo funcionan las sEctas, qué técnicas emplean y cómo se comportan los líderes sectarios. También se describen algunos aspectos de la evaluación pericial de las víctimas, algunos instrumentos específicos del abuso psicológico grupal y el resumen de las causas judiciales que conllevaron a la condena de su líder. Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.
- Published
- 2018
16. Coumarin: a novel player in microbial quorum sensing and biofilm formation inhibition
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Reen, F. Jerry, Gutierrez-Barranquero, Jose A., Parages, Maria L., and O'Gara, Fergal
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Natural products ,Anti-infectives ,Quorum sensing inhibition ,Coumarin ,Antibiofilm - Abstract
Antibiotic resistance is a growing threat worldwide, causing serious problems in the treatment of microbial infections. The discovery and development of new drugs is urgently needed to overcome this problem which has greatly undermined the clinical effectiveness of conventional antibiotics. An intricate cell-cell communication system termed quorum sensing (QS) and the coordinated multicellular behaviour of biofilm formation have both been identified as promising targets for the treatment and clinical management of microbial infections. QS systems allow bacteria to adapt rapidly to harsh conditions, and are known to promote the formation of antibiotic tolerant biofilm communities. It is well known that biofilm is a recalcitrant mode of growth and it also increases bacterial resistance to conventional antibiotics. The pharmacological properties of coumarins have been well described, and these have included several that possess antimicrobial properties. More recently, reports have highlighted the potential role of coumarins as alternative therapeutic strategies based on their ability to block the QS signalling systems and to inhibit the formation of biofilms in clinically relevant pathogens. In addition to human infections, coumarins have also been found to be effective in controlling plant pathogens, infections in aquaculture, food spoilage and in reducing biofouling caused by eukaryotic organisms. Thus, the coumarin class of small molecule natural product are emerging as a promising strategy to combat bacterial infections in the new era of antimicrobial resistance.
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- 2018
17. Estudio comparativo de caracteristicas de las cepas de Pseudomonas syringae pv. syringae asociadas a mango
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Aprile, Francesca, Gutiérrez-Barranquero, Jose A., Cazorla, Francisco M., and de Vicente, Antonio
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Mango ,Pseudomonas syringae ,Microbiología ,Patogenesis - Abstract
Pseudomonas syringae es una bacteria fitopatógena modelo, incluyendo aspectos sobre evolución microbiana y de la que se dispone de una gran cantidad de información genómica. Esta especie está compuesta por más de 60 patovares diferentes, siendo el patovar syringae uno de los más importantes. P. syringae pv. syringae (Pss) es el agente causal de la necrosis apical del mango, enfermedad que afecta a las principales áreas de cultivo de clima mediterráneo a nivel mundial. El objetivo principal de este trabajo es el estudio epidemiológico y evolutivo sobre la adaptación de la bacteria al ambiente y a su huésped. Para ello, se llevará a cabo la comparación de dos colecciones de cepas de Pss aisladas de mango en diferentes zonas geográficas, y separadas dos décadas en el tiempo. Ambas colecciones se han caracterizado desde el punto de vista fenotípico y genético en cuanto a sus características más relevantes implicadas tanto es su fase epifítica como patogénica. También se ha analizado la filogenia de las cepas incluidas en nuestro estudio, empleando secuencias parciales de dos genes housekeeping (gyrB y rpoD). Los resultados nos han permitido seleccionar un número representativo de cepas a las que se realizarán la secuenciación de sus genomas, y así poder llevar a cabo posteriormente estudios de genómica comparada que nos permitan descifrar los procesos evolutivos involucrados en la adaptación de las poblaciones de Pss al mango. Este trabajo ha sido financiado por ayuda de la Consejería de Economía, Innovación y Ciencia, Junta de Andalucía (P12-AGR-1473), cofinanciados con fondos FEDER (UE) y la Universidad de Málaga.
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- 2018
18. Isolation, characterization and selection of bacterial isolates from a suppressive soil with beneficial traits to plants
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Vida, Carmen, Tienda, Sandra, De Vicente, Antonio, Cazorla, Francisco M., and Gutiérrez-Barranquero, Jose A.
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Aguacate ,Hongos fitopatógenos ,Biocontrol ,Rosellinia necatrix - Abstract
Backgrounds This study focused on the characterization and selection of bacterial strains obtained from a suppressive soil displaying antifungal activity against the soilborne phytopathogenic fungi Rosellinia necatrix. Bacterial profile from this suppressive soil were first obtained by 16S rRNA gene sequencing, revealing a significant increase in the bacterial class Gammaproteobacteria, especially in some antagonistic representatives of Pseudomonas spp. Objectives To obtain and characterize a collection of 246 bacterial isolates obtained from this suppressive soil, in order to identify new strains with antifungal activity against fungal phytopathogens. Methods To obtain the bacterial collection, we performed an isolation on a selective medium for Pseudomonas-like microorganisms. Further characterization tests were used in order to analyse the bacterial collection, including identification of the general metabolic profile of glucose, the profiling of antifungals produced, including both the putative production of antifungal compounds and lytic exoenzymes, and the evaluation of traits related with beneficial effects on plants. Conclusions A final selection of representative strains resulted in antifungal isolates belonging to the genus Pseudomonas, but also some representatives of the genera Serratia and Stenotrophomonas. These selected strains were tested for plant protection by an in vivo experiment using avocado and wheat plants challenged by the pathogen R. necatrix, showing all of them an antifungal ability and plant disease protection. Pseudomonas-like strains isolated from suppressive soils constitute an excellent source for novel microbial biocontrol agents against soilborne fungal pathogens. This work was supported by grant AGL2014-52518-C2-1-R. Carmen Vida and Sandra Tienda are supported by a PhD fellowship from the FPI program of the Spanish Government. This work was supported by grant AGL2014-52518-C2-1-R. Carmen Vida and Sandra Tienda are supported by a PhD fellowship from the FPI program of the Spanish Government; Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.
- Published
- 2017
19. Assessment of coercive persuasion: the Scale of Detection of Coercive Persuasion in Group Contexts (EDPC)
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Cuevas-Barranquero, Jose Miguel, Almendros-Rodríguez, Carmen, and Canto-Ortiz, Jesus Maria
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Persuasión (Psicología) ,Influence ,Persuasion ,Cults ,Assessment ,Coercive persuasion ,Abuse ,Scale - Abstract
Comunicación presentada en las Jornadas Internacionales ICSA 2017 sobre Dinámicas sectarias y radicalización Coercive persuasion refers to the control and manipulation developed by abusive groups, through different aggressive strategies that influence changes in the environment of its members, distorting cognition, altering emotions and generating significant psychosocial damage. It is a subtle, gradual and powerful force that affects around 500,000 Spanish victims of cultic groups (Cuevas & Perlado, 2011; Cuevas, 2012). Attaining power is one of the main goals of these groups, being the control and exploitation of the individual a part of the process. This derives to individuals giving up their own goals, freedom, material possessions, family and social networks, health or even life itself (Rodríguez-Carballeira, Saldaña, Almendros, Martin-Peña, Escartín, & Porrúa-Garcia, 2015). Such strategies are often implemented in a planned, graduate way and using deceit, difficulting that people who targeted are able to detect their evident aggressiveness and the generated damage. If there is an obvious shortage of instruments measuring psychological abuse in different fields (partner violence, harassment, bullying, etc.), the development of tools to assess the presence of such strategies in group contexts is even more scarce (Almendros, Gámez-Guadix, Carrobles & Rodríguez Carballeira, 2011). One of those assessment tools, the Interview for Detection of Coercive Persuasion (Cuevas & Canto, 2006) contains a wide range of coercive and abusive practises taking place within manipulative group. It has been applied in Spain in the forensic field in prosecutions of abusive groups (Dharma Tradición, Casa Yoga, Miguelianos, Revelance, etc.). The main objective of this recently validated tool (Cuevas, 2016) was to identify and provide evidences of the systematic application of coercive persuasion techniques on victims of abusive groups (Cuevas, 2012, 2016). Deriving from this instrument, sharing objectives, a new scale of 40 items and validated in Spanish population: the Scale of Detection of Coercive Persuasion in Group Contexts, or EDPC (Cuevas, 2016 ). To validate the EDPC, a Spanish sample of 134 people who identified themselves as having been abused or having been overly controlled by a group was selected. To assess criterion validity of the instrument, other different instruments (BSI MOS-SSS, RSE, SLEQ, ICP and EDS) were used. The group psychological abuse scale GPA (Chambers, Langone, Dole, & Grice, 1994), Spanish modified version (Almendros et al.,2004; Almendros et al., 2009) was used to assess the convergent validity of the instrument. The EDPC showed appropriate psychometric properties. In respect to reliability, the standardized Cronbach alpha coefficient reached a value of 0.97. The exploratory factorial analysis indicated the presence of a factor (coercive persuasion), establishing the suitability of a one-dimensional model. This scale aims to be useful in clinical and forensic fields, in order to assess the control and manipulation exercised in group contexts. Using it could be relevant to provide evidences of coercive groups practises, helping at trying to determinate the relationship between damage on the victims and the specific actions taken by groups or individuals who perform the abusive behaviors. Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.
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- 2017
20. Escala de evaluación de persuasión coercitiva en contextos grupales
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Cuevas-Barranquero, Jose Miguel, Canto-Ortiz, Jesus Maria, and Almendros-Rodríguez, Carmen
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Dominio (Psicología) ,Evaluación ,Abuso psicológico ,Sectas ,Persuasión coercitiva ,Grupos - Abstract
Introducción: La persuasión coercitiva puede emplearse con distintos fines: explotación económica, control o poder, satisfacción sexual, etc. Se trataría de una forma de influencia social extrema, que puede causar importantes daños psicosociales: trastornos o lesiones psicológicas, daño moral, alteración de la capacidad volitiva, afectación de las redes socio-familiares, pérdidas materiales, intensificar problemas de salud preexistentes, o incluso la pérdida de la misma vida (Rodríguez-Carballeira, Saldaña, Almendros, Martín-Peña, Escartín y Porrúa-García, 2015). Uno de los escasos instrumentos validados, la Entrevista de Persuasión Coercitiva (Cuevas, 2016), recoge una amplia gama de coerciones y abusos desarrollados en contextos grupales manipulativos. En España se ha aplicado en procesos forenses relacionados con victimización de grupos abusivos. Su principal objetivo es identificar y constatar si se han aplicado técnicas de persuasión coercitiva sobre la víctima evaluada, relacionándolas con los consecuentes daños psicológicos. A partir de ésta derivaron los ítems de la Escala de Detección de Persuasión Coercitiva en Contextos Grupales (EDPC). Método: Método 1. Participantes: 134 españoles, autoidentificados como víctimas de abuso psicológico grupal. 69 varones (51,5%) y 65 mujeres (48,5%). Edad media de 48,85 años (DT = 11,91). 2. Instrumentos para la validación: Cuestionario sociodemográfico, Información sobre experiencias grupales, Información tras la salida del grupo Escala de abuso psicológico grupal (GPA, Chambers Langone, Dole y Grice, 1994) Medical Outcomes Study Support Survey (MOS-SSS, Sherbourne y Stewart, 1991) Stressful Life Event Questionnaire (SLQ, Roohafza, Ramezani, Sadeghi, Shanam, Zolfagari y Sarafzadegan, 2011) Escala de Autoestima (RSE, Rosenberg, 1965) Brief Symptoms Inventory (BSI, Derogatis, 1975) Inventario de Cogniciones Postraumáticas (ICP, Foa, Ehlers, Clark, Toliln y Orsillo, 1999) Escala de Deseabilidad Social (SDS, Crowne y Marlon, 1960) . 3. Procedimiento Base de datos Proquest, accesible a través de internet. Invitaciones para participar dirigidas a ex miembros de grupos manipulativos. A través de asociaciones, profesionales y foros especializados, difusión en medios de comunicación , etc. del 23/07/15—30/09/2015. 4. Plan de Ánálisis Se examinan las características psicométricas de la escala: pruebas paramétricas y no paramétricas para evaluar diferencias grupales, análisis descriptivo de los ítems, consistencia interna y su estructura, a través de análisis factorial. Resultados: Resultados Sus propiedades psicométricas han sido adecuadas. FIABILIDAD: coeficiente alfa de Cronbach estandarizado = 0,97. ANÁLISIS FACTORIAL: presencia de un factor (persuasión coercitiva), estableciendo la idoneidad de un modelo unidimensional. VALIDEZ CONVERGENTE: las correlaciones entre la EDPC y la GPA son muy elevadas y significativas (p
- Published
- 2016
21. Pantoea agglomerans as a New Etiological Agent of a Bacterial Necrotic Disease of Mango Trees.
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Gutierrez-Barranquero, Jose A., Cazorla, Francisco M., Tores, Juan Antonio, and de Vicente, Antonio
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- *
PANTOEA agglomerans , *NECROSIS , *MICROBIAL toxins , *PLANTS - Abstract
Bacterial apical necrosis of mango trees, a disease elicited by Pseudomonas syringae pv. syringae, is a primary limiting factor of mango crop production in the Mediterranean region. In this study, a collection of bacterial isolates associated with necrotic symptoms in mango trees similar to those produced by bacterial apical necrosis disease were isolated over five consecutive years in orchards from the Canary Islands. The bacteria] isolates were characterized and identified as Pantoea agglomerans. Pathogenicity tests conducted on onion bulbs and mango plants confirmed that P. agglomerans strains isolated from mango trees are a new etiological agent of a bacterial necrotic disease in the Canary Islands. Pathogenicity plasmids of the pPATH family have been previously reported in P. agglomerans. The majority of putatively pathogenic (n = 23) and pathogenic (n = 4) P. agglomerans strains isolated from mango trees harbored four plasmids, one of which was close in size to the 135-kb pPATH pathogenicity plasmid. The analysis of the presence of two major genes in pPATH plasmids (repA and hrpj) was undertaken in P. agglomerans strains isolated from mango trees. The hrpj gene was detected in the 140-kb plasmid of pathogenic P. agglomerans strains isolated from mango trees but it showed differences in nucleotide sequences compared with other pathogenic strains. In contrast, the repA gene was not detected in any of the putatively pathogenic and pathogenic P. agglomerans strains isolated from mango trees. Finally, genetic characterization and phylogenetic analysis using the hrpj gene and the housekeeping genes gyrB and rpoB showed that almost all P. agglomerans strains that were putatively pathogenic and pathogenic on mango trees clustered together, forming a differentiated phylogroup with respect to the other pathogenic P. agglomerans strains described from other hosts. [ABSTRACT FROM AUTHOR]
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- 2019
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22. Multimodal Optimization: An Effective Framework for Model Calibration
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Chica, Manuel, primary and Barranquero, Jose, additional
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- 2016
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23. Multiclass Support Vector Machines With Example-Dependent Costs Applied to Plankton Biomass Estimation
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Gonzalez, Pablo, primary, Alvarez, Eva, additional, Barranquero, Jose, additional, Diez, Jorge, additional, Gonzalez-Quiros, Rafael, additional, Nogueira, Enrique, additional, Lopez-Urrutia, Angel, additional, and del Coz, Juan Jose, additional
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- 2013
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24. Mining Microbial Signals for Enhanced Biodiscovery of Secondary Metabolites.
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Reen FJ, Gutiérrez-Barranquero JA, and O'Gara F
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- Biosensing Techniques, Chromatography, High Pressure Liquid, Gene Library, High-Throughput Screening Assays, Mass Spectrometry, Metabolomics methods, Metagenomics methods, Quorum Sensing genetics, Biodiversity, Data Mining methods, Microbial Interactions, Microbiota, Secondary Metabolism, Signal Transduction
- Abstract
The advent of metagenomics based biodiscovery has provided researchers with previously unforeseen access to the rich tapestry of natural bioactivity that exists in the biosphere. Unhindered by the "culturable bottleneck" that has severely limited the translation of the genetic potential that undoubtedly exists in nature, metagenomics nonetheless requires ongoing technological developments to maximize its efficacy and applicability to the discovery of new chemical entities.Here we describe methodologies for the detection and isolation of quorum sensing (QS) signal molecules from metagenomics libraries. QS signals have already shown considerable potential for the activation and "awakening" of biosynthetic gene clusters, bridging the existing divide between the natural product repertoire and the natural biosynthetic biodiversity hinted at by nature's blueprint. The QS pipeline from high-throughput robotics to functional screening and hit isolation is detailed, highlighting the multidisciplinary nature of progressive biodiscovery programs.
- Published
- 2017
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