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4. Multifunctional Antimicrobial Nanofiber Dressings Containing ε-Polylysine for the Eradication of Bacterial Bioburden and Promotion of Wound Healing in Critically Colonized Wounds

Author

Mayandi, Venkatesh, primary, Wen Choong, Alvin Chua, additional, Dhand, Chetna, additional, Lim, Fui Ping, additional, Aung, Thet Tun, additional, Sriram, Harini, additional, Dwivedi, Neeraj, additional, Periayah, Mercy Halleluyah, additional, Sridhar, Sreepathy, additional, Fazil, Mobashar Hussain Urf Turabe, additional, Goh, Eunice Tze Leng, additional, Orive, Gorka, additional, W. Beuerman, Roger, additional, Barkham, Timothy Mark Sebastian, additional, Loh, Xian Jun, additional, Liang, Zhao-Xun, additional, Barathi, Veluchamy Amutha, additional, Ramakrishna, Seeram, additional, Chong, Si Jack, additional, Verma, Navin Kumar, additional, and Lakshminarayanan, Rajamani, additional

Published
2020
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5. Protective Action of Linear Polyethylenimine against Staphylococcus aureus Colonization and Exaggerated Inflammation in Vitro and in Vivo

Author

Mayandi, Venkatesh, primary, Sridhar, Sreepathy, additional, Fazil, Mobashar Hussain Urf Turabe, additional, Goh, Eunice Tze Leng, additional, Htoon, Hla Myint, additional, Orive, Gorka, additional, Choong, Yeu Khai, additional, Saravanan, Rathi, additional, Beuerman, Roger W., additional, Barkham, Timothy Mark Sebastian, additional, Yang, Liang, additional, Baskaran, Mani, additional, Jhanji, Vishal, additional, Loh, Xian Jun, additional, Verma, Navin Kumar, additional, and Lakshminarayanan, Rajamani, additional

Published
2019
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6. Antimicrobial Activity and Cell Selectivity of Synthetic and Biosynthetic Cationic Polymers

Author

Venkatesh, Mayandi, primary, Barathi, Veluchamy Amutha, additional, Goh, Eunice Tze Leng, additional, Anggara, Raditya, additional, Fazil, Mobashar Hussain Urf Turabe, additional, Ng, Alice Jie Ying, additional, Harini, Sriram, additional, Aung, Thet Tun, additional, Fox, Stephen John, additional, Liu, Shouping, additional, Yang, Liang, additional, Barkham, Timothy Mark Sebastian, additional, Loh, Xian Jun, additional, Verma, Navin Kumar, additional, Beuerman, Roger W., additional, and Lakshminarayanan, Rajamani, additional

Published
2017
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8. Multifunctional Antimicrobial Nanofiber Dressings Containing ε-Polylysine for the Eradication of Bacterial Bioburden and Promotion of Wound Healing in Critically Colonized Wounds

Author

Mayandi, Venkatesh, Wen Choong, Alvin Chua, Dhand, Chetna, Lim, Fui Ping, Aung, Thet Tun, Sriram, Harini, Dwivedi, Neeraj, Periayah, Mercy Halleluyah, Sridhar, Sreepathy, Fazil, Mobashar Hussain Urf Turabe, Goh, Eunice Tze Leng, Orive, Gorka, W. Beuerman, Roger, Barkham, Timothy Mark Sebastian, Loh, Xian Jun, Liang, Zhao-Xun, Barathi, Veluchamy Amutha, Ramakrishna, Seeram, Chong, Si Jack, Verma, Navin Kumar, and Lakshminarayanan, Rajamani

Abstract

Bacterial colonization of acute and chronic wounds is often associated with delayed wound healing and prolonged hospitalization. The rise of multi-drug resistant bacteria and the poor biocompatibility of topical antimicrobials warrant safe and effective antimicrobials. Antimicrobial agents that target microbial membranes without interfering with the mammalian cell proliferation and migration hold great promise in the treatment of traumatic wounds. This article reports the utility of superhydrophilic electrospun gelatin nanofiber dressings (NFDs) containing a broad-spectrum antimicrobial polymer, ε-polylysine (εPL), crosslinked by polydopamine (pDA) for treating second-degree burns. In a porcine model of partial thickness burns, NFDs promoted wound closure and reduced hypertrophic scarring compared to untreated burns. Analysis of NFDs in contact with the burns indicated that the dressings trap early colonizers and elicit bactericidal activity, thus creating a sterile wound bed for fibroblasts migration and re-epithelialization. In support of these observations, in porcine models of Pseudomonas aeruginosaand Staphylococcus aureuscolonized partial thickness burns, NFDs decreased bacterial bioburden and promoted wound closure and re-epithelialization. NFDs displayed superior clinical outcome than standard-of-care silver dressings. The excellent biocompatibility and antimicrobial efficacy of the newly developed dressings in pre-clinical models demonstrate its potential for clinical use to manage infected wounds without compromising tissue regeneration.

Published
2024
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9. Enterococcus faecalis Modulates Immune Activation and Slows Healing During Wound Infection.

Author

Kian Long Chong, Kelvin, Wei Hong Tay, Janela, Baptiste, Mei Hui Yong, Adeline, Tze Horng Liew, Madden, Leigh, Keogh, Damien, Barkham, Timothy Mark Sebastian, Ginhoux, Florent, Becker, David Laurence, Kline, Kimberly A., Chong, Kelvin Kian Long, Tay, Wei Hong, Yong, Adeline Mei Hui, and Liew, Tze Horng

Subjects
ENTEROCOCCUS faecalis, BACTERIAL diseases, WOUND infections, WOUND healing, CYTOKINES, IMMUNE system physiology, ANIMAL experimentation, BIOLOGICAL models, COMPARATIVE studies, ENTEROCOCCUS, RESEARCH methodology, MEDICAL cooperation, MENTAL health surveys, MICE, RESEARCH, GRAM-positive bacterial infections, EVALUATION research
Abstract

Enterococcus faecalis is one of the most frequently isolated bacterial species in wounds yet little is known about its pathogenic mechanisms in this setting. Here, we used a mouse wound excisional model to characterize the infection dynamics of E faecalis and show that infected wounds result in 2 different states depending on the initial inoculum. Low-dose inocula were associated with short-term, low-titer colonization whereas high-dose inocula were associated with acute bacterial replication and long-term persistence. High-dose infection and persistence were also associated with immune cell infiltration, despite suppression of some inflammatory cytokines and delayed wound healing. During high-dose infection, the multiple peptide resistance factor, which is involved in resisting immune clearance, contributes to E faecalis fitness. These results comprehensively describe a mouse model for investigating E faecalis wound infection determinants, and suggest that both immune modulation and resistance contribute to persistent, nonhealing wounds. [ABSTRACT FROM AUTHOR]

Published
2017
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