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208 results on '"Barinov, N."'

3. Ti2NiCu Based Composite Nanotweezers with a Shape Memory Effect and its Use for DNA Bunches 3D Manipulation

Author

Orlov, A. P., Frolov, A. V., Smolovich, A. M., Lega, P. V., Chung, P. V., Irzhak, A. V., Barinov, N. A., Klinov, D. V., Vlasenko, V. S., and Koledov, V. V.

Subjects
Physics - Biological Physics, Physics - Applied Physics
Abstract

The DNA molecules were controllable deposited on graphene and thin graphite films and visualized using AFM. The mechanical micro- and nanotools, such as nanotweezers with shape memory effect controlled by heating were designed and tested. A technique for fabricating a structure with the inclusion of suspended DNA threads and manipulating those using composite nanotweezers with shape memory effect was suggested., Comment: arXiv admin note: text overlap with arXiv:1811.02943

Published
2019
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4. Deposition and Visualization of DNA Molecules on Graphene That is Obtained with the Aid of Mechanical Splitting on a Substrate with an Epoxy Sublayer

Author

Frolov, A. V., Barinov, N. A., Klinov, D. V., Koledov, V. V., Lega, P. V., Orlov, A. P., and Smolovich, A. M.

Subjects
Physics - Biological Physics, Physics - Applied Physics
Abstract

Controlled deposition of DNA on graphene films obtained with the aid of mechanical splitting of graphite on a substrate with an epoxy sublayer is demonstrated. The DNA molecules are visualized using AFM.

Published
2018
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43. Morphometric characterization of fibrinogen's αc regions and their role in fibrin self-assembly and molecular organization

Author

Protopopova A., Litvinov R., Galanakis D., Nagaswami C., Barinov N., Mukhitov A., Klinov D., and Weisel J.

Abstract

© 2017 The Royal Society of Chemistry. The flexible C-terminal parts of fibrinogen's Aα chains named the αC regions have been shown to play a role in fibrin self-assembly, although many aspects of their structure and functions remain unknown. To examine the involvement of the αC regions in the early stages of fibrin formation, we used high-resolution atomic force microscopy to image fibrinogen and oligomeric fibrin. Plasma-purified full-length human fibrinogen or des-αC fibrinogen lacking most of the αC regions, untreated or treated with thrombin, was imaged. Up to 80% of the potentially existing αC regions were visualized and quantified; they were highly heterogeneous in their length and configurations. Conversion of fibrinogen to fibrin was accompanied by an increase in the incidence and length of the αC regions as well as transitions from more compact conformations, such as a globule on a string, to extended and more flexible offshoots. Concurrent dynamic turbidimetry, confocal microscopy, and scanning electron microscopy revealed that trimming of the αC regions slowed down fibrin formation, which correlated with longer protofibrils, thinner fibers, and a denser network. No structural distinctions, except for the incidence of the αC regions, were revealed in the laterally aggregated protofibrils made of the full-length or des-αC fibrinogens, suggesting a pure kinetic effect of the αC regions on the fibrin architecture. This work provides a structural molecular basis for the promoting role of the αC regions in the early stages of fibrin self-assembly and reveals this stage of fibrin formation as a potential therapeutic target to modulate the structure and mechanical properties of blood clots.

Published
2017

46. Ti2NiCu Based Composite Nanotweezers with a Shape Memory Effect and its Use for DNA Bunches 3D Manipulation.

Author

Orlov, A. P., Frolov, A. V., Smolovich, A. M., Lega, P. V., Chung, P. V., Irzhak, A. V., Barinov, N. A., Klinov, D. V., Vlasenko, V. S., and Koledov, V. V.

Subjects
GRAPHENE, CARBON films, SHAPE memory effect, TITANIUM alloys, NICKEL alloys, COPPER alloys, NANOELECTROMECHANICAL systems, NANOCOMPOSITE materials
Abstract

The DNA molecules were controllable deposited on graphene and thin graphite films and visualized using AFM. The mechanical micro- and nanotools, such as nanotweezers with shape memory effect controlled by heating were designed and tested. A technique for fabricating a structure with the inclusion of suspended DNA threads and manipulating those using composite nanotweezers with shape memory effect was suggested. [ABSTRACT FROM AUTHOR]

Published
2019
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50. The structural diversity of C-rich DNA aggregates: unusual self-assembly of beetle-like nanostructures.

Author

ProtopopovaCurrently at University of Pennsylvania School of Medicine Philadelphia PA USA., A. D., Tsvetkov, V. B., Varizhuk, A. M., Barinov, N. A., Podgorsky, V. V., Klinov, D. V., and Pozmogova, G. E.

Abstract

We studied the ability of oligonucleotides CnT25 (n = 2, 5, 7, 9, 12, 25) to form an intermolecular i-motif using circular dichroism, ultra-violet spectroscopy, nuclear magnetic resonance, high-resolution atomic force microscopy, high-performance liquid chromatography, and molecular dynamics simulations. The arrangement of single-stranded oligonucleotides in multimer i-motifs was very unusual: C-tracts of different oligonucleotides followed each other consecutively in order to fold into a closed intermolecular i-motif core with minimal loops (one cytidine in a loop spanning over a minor groove, three cytidines in a loop over a major groove); intact T-tracts protruded from predefined loci allowing visualization of beetle-like nanostructures by atomic force microscopy. The same structures were formed from analogous biotinylated oligonucleotides demonstrating one of the potential applications of such structures as carriers of multiple functional groups. Our findings open up possibilities for the rational design of pH-sensitive DNA aggregates and evaluation of the efficiency of their assembly. [ABSTRACT FROM AUTHOR]

Published
2018
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