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Assisted reproduction techniques ofer a solution to infertility problems in the population. The usual method to evaluate embryo quality in order to select the best embryo is the visualization of its morphological parameters. Through time-lapse technology, it has been possible to carry out a study about the infuence of embryo collapses, whose objective is to fnd out if it could be considered as another parameter in embryo assessment. Such collapses arise normal and randomly in the blastocysts and appear to be negatively related to implantation. In addition, it has been confrmed that they contribute to a slightly delayed development in the embryo, which could be related to implantation failure. Finally, although this parameter is not included in ASEBIR criteria for embryo classifcation, the study supports that it could be considered as a relevant criterion during embryo selection to increase success in implantation rates and live new-born., Las técnicas de reproducción asistida ofrecen una solución a los problemas de infertilidad en la población. El método habitual para evaluar la calidad embrionaria con la fnalidad de seleccionar el mejor embrión es la visualización de sus parámetros por morfología clásica. Mediante tecnología time-lapse se ha podido llevar a cabo un estudio sobre la infuencia de los colapsos embrionarios, cuyo objetivo es averiguar si éstos podrían considerarse un parámetro más en la valoración embrionaria. Dichos colapsos surgen de manera habitual y azarosa en los blastocistos y parece ser que están relacionados negativamente con la tasa de implantación. Además, se ha confrmado que contribuyen a un desarrollo levemente retardado en el embrión lo que podría estar relacionado con en el fallo de implantación. Finalmente, a pesar de que este parámetro no está incluido en los criterios ASEBIR de clasifcación embrionaria, el estudio apoya que podría considerarse como criterio relevante durante la selección del embrión para incrementar el éxito en las tasas de implantación y recién nacido vivo.

A survey of the natural mycorrhizal potential has been carried out in a representative area of a desertified semiarid ecosystem in the southeast of Spain, Many indigenous plants from the field site were mycorrhizal, including the dominant Anthyllis cytisoides, which had high levels of colonization by arbuscular mycorrhizal fungi (AMF), Low numbers of AMF spores were present in the soil, although a range of species, including Scutellospora calospora, Glomus coronatum, Glomus constrictum, and several Acaulospora species, was represented, Soil infectivities, as determined by a soil dilution method; were similar for most plants tested but were significantly lower for Anthyllis cytisoides. Nevertheless, when a less disruptive method to determine soil infectivity was used, the importance of the mycelial network in maintaining the infectivity of soil under perennial shrubs, such as Anthyllis cytisoides, was highlighted, Seasonal variations in the mycorrhizal infectivity showed that it was higher towards the end of the summer period than in midwinter. In screening trials in a greenhouse, the indigenous AMF did not significantly improve the growth of plants compared with that of noninoculated controls, Augmentation of the soil with an inoculum of Glomus intraradices resulted in improved growth of Anthyllis cytisoides in both sterile and nonsterile conditions, in contrast to results obtained following inoculation with Glomus mosseae or another Glomus sp, Our findings suggest that the indigenous inoculum levels of AMF are inadequate to support an extensive revegetation program in the absence of an additional mycorrhizal inoculum.

he fundamental importance of microbial processes for the efficient cycling of mineral nutrients within biosphere has been realized for many years (fig.1), and the involvement of the arbuscular mycorrhizal fungi (AMF) fully recognized.