1. Detection and selection of microsatellites in the genome of Paracoccidioides brasiliensis as molecular markers for clinical and epidemiological studies.
- Author
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Nascimento E, Martinez R, Lopes AR, de Souza Bernardes LA, Barco CP, Goldman MH, Taylor JW, McEwen JG, Nobrega MP, Nobrega FG, and Goldman GH
- Subjects
- Animals, Armadillos microbiology, Genome, Fungal, Humans, Paracoccidioides genetics, Paracoccidioidomycosis epidemiology, Paracoccidioidomycosis microbiology, Phylogeny, Polymerase Chain Reaction, Virulence, Genetic Markers, Microsatellite Repeats genetics, Paracoccidioides classification, Paracoccidioides pathogenicity, Paracoccidioidomycosis physiopathology
- Abstract
Paracoccidioides brasiliensis, a thermodimorphic fungus, is the causative agent of the prevalent systemic mycosis in Latin America, paracoccidioidomycosis (PCM). Here, we describe the microsatellite patterns observed in a collection of P. brasiliensis random sequence tags. We identified 1,117 microsatellite patterns in about 3.8 Mb of unique sequences (0.47% of the total DNA used in the analysis). The majority of these microsatellites (87.5%) are found in noncoding sequences. We used two polymorphic microsatellites located on noncoding and coding sequences, as well as two microsatellites located on introns, as molecular markers to discriminate P. brasiliensis isolates, to look for relationships between the genetic background of the strains and the types of human disease they cause. We did not observe any correlation between the clinical form of human PCM and four simple sequence repeat patterns analyzed.
- Published
- 2004
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