1. EFFECTS OF OXYTETRACYCLINE SUPPLEMENTATION ON CRYOPRESERVED SPERM QUALITY OF SHABOUT (BARBUS GRYPUS HECKEL 1843): APOPTOTIC ANALYSIS, DNA DAMAGE AND OXIDATIVE STRESS.
- Author
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DOĞU, Z., ŞAHINÖZ, E., ARAL, F., KOYUNCU, I., and EĞI, K.
- Subjects
OXYTETRACYCLINE ,DNA damage ,OXIDATIVE stress ,SPERMATOZOA ,SEMEN analysis ,FROZEN semen ,SPERM motility - Abstract
The study aimed to examine the effects of oxytetracycline supplementation to glucose extender on the Oxidative Status, Glutathione (GSH), Malondialdehyde (MDA), 8-Hydroxydeoxyguanosine (8-OHdG) and Apoptotic Spermatozoa of Barbus grypus post-thaw sperm. The semen was frozen in diluents containing three different oxytetracycline concentrations (0.78, 1.56 and 3.12 mg). All the comparable levels of oxytetracycline linearly improved the post-thaw sperm motility rate and duration significantly (p< 0.0001). MDA and Total Oxidative Status (TOS) were linearly and quadratically decreased, however, Total Antioxidative Status (TAS) quadratically and GSH linearly and quadratically increased with oxytetracycline (p < 0.001). The increasing oxytetracycline levels typically resulted in a linear decline in DNA damage in the 8-OHdG assay. The determined percentage of apoptotic spermatozoa were linearly and quadratically reduced with Oxytetracycline (OTC) (p< 0.0001). These results showed that oxytetracycline can be used as an antibiotic additive in semen extender, providing better sperm freezing-thawing, without decreasing semen quality and antioxidant and increasing the oxidant, DNA damage and apoptotic sperm level of the Barbus grypus. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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