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8 results on '"Barbosa, LMR"'

1. Metabolizable Energy Levels for Meat-Type Quails at Starter Phase

Author

Muniz, JCL, Barreto, SLT, Viana, GS, Mencalha, R, Reis, RS, Hannas, MI, Barbosa, LMR, and Maia, RC

Subjects
Coturnix coturnix coturnix, food and beverages, Carcass traits, performance
Abstract

This experiment was conducted to evaluate the effects of dietary nitrogen-corrected apparent metabolizable energy (AMEn) levels on meat-type quail performance and carcass traits from 1 to 14d of age. A total of 1120 not sexed meat-type quails were randomly distributed to seven treatments, with eight replicates with 20 quails each. A basal corn and soybean meal-based diet was formulated to meet or exceed quail nutritional requirements, except for ME. Graded levels of soybean oil were added to the basal diet in replacement, to sand, to obtain dietary treatments (2,600; 2,700; 2,800; 2,900; 3,000; 3,100 and 3,200 KcalAMEn/kg diet). Data were analyzed as one-way ANOVA and optimum AMEn levels were estimated using polynomial regression model. Increasing in dietary AMEn levels elicited a linear decrease (p0.05) by AMEn. Based on the results, the dietary AMEn level that warrants adequate performance and carcass traits of meat-type quails from 1 to 14d of age is 2,820 Kcal/kg diet.

Published
2018

2. Digestible Methionine + Cysteine-to-Lysine Ideal Ratio for Meat-Type Quails at Initial Phase

Author

Reis, RS, Viana, GS, Barreto, SLT, Muniz, JCL, Mendonça, MO, Mencalha, R, Ribeiro, CLN, and Barbosa, LMR

Subjects
Coturnix coturnix coturnix, digestible amino acids, Sulphur amino acids
Abstract

This trial was performed to determine the ideal ratio of digestible methionine + cysteine (Met/Lys) with digestible lysine for meat-type quails from 1 to 14 days of age. A total of six hundred thirty, one-day-old, not sexed meat type quails were randomly assigned to six groups of treatments, each one with seven replicates and fifteen quails per experimental unit. A basal diet, methionine + cysteine-deficient, was graded supplemented with DL-Methionine (0.108; 0.169; 0.230; 0.291; 0.352 and 0.413%) to obtain the treatments, represented by six digestible Met/Lys ratios (0.60; 0.65; 0.70; 0.75; 0.80 and 0.85). Data was analyzed using ANOVA and polynomial regression and broken-line models were used to estimate the digestible Met/Lys ideal ratio, considering adjust of data to both models. Statistical significance was considered when p≤0.05. Feed intake, weight gain and final body weight was linearly increased as dig. Met/Lys ratio increased. Feeding conversion was improved with increasing in dig. Met/Lys ratios. A quadratic response on feathering percentage was observed with increasing dig. Met/Lys ratios. Digestible Met/Lys ratio which, maximized feathering percentage in meat-type quails, was 0.74. According to linear broken-line model, dig. Met/Lys ratio estimated for optimum feed intake, weight gain, final body weight and feeding conversion were, respectively, 0.66; 0.71; 0.71 and 0.74. The dig. Met/Lys ideal ratio for meat-type quails from one to 14 days of age is 0.74.

Published
2017

5. Effects of crude protein and lactose levels in diets on growth performance, intestinal morphology, and expression of genes related to intestinal integrity and immune system in weaned piglets.

Author

Soares MH, Rodrigues GA, Barbosa LMR, Valente Júnior DT, Santos FC, Rocha GC, Campos PHRF, and Saraiva A

Subjects
Animals, Eating, Female, Male, Occludin genetics, Occludin metabolism, Swine anatomy & histology, Swine immunology, Tight Junctions genetics, Tight Junctions metabolism, Weight Gain, Animal Feed analysis, Animal Nutritional Physiological Phenomena, Diet veterinary, Dietary Proteins analysis, Gene Expression, Intestinal Mucosa anatomy & histology, Intestinal Mucosa immunology, Lactose analysis, Swine genetics, Swine growth & development, Transforming Growth Factor beta1 genetics, Transforming Growth Factor beta1 metabolism
Abstract

To evaluate the effects of crude protein (CP) and lactose (LAC) for weaned piglets on performance, intestinal morphology, and expression of genes related to intestinal integrity and immune system, 144 piglets with initial weight 7.17 ± 0.97 kg were allotted in a randomized design, in a 2 × 3 factorial arrangement (20.0% and 24.0% CP and 8.0%, 12.0%, and 16.0% LAC) with eight replicates. Piglets fed 20.0% CP had greater weight gain and feed intake. Including 12.0% LAC in the 20.0% CP diet provided higher villous height in the duodenum than 8.0% LAC, and 12.0% or 16.0% LAC in the 24.0% CP diet resulted in higher villous height in the jejunum and ileum, and higher villi/crypt ratio in the ileum than 8.0% LAC. No effects of CP and LAC on interleukin-1β and tumor necrosis factor-α mRNA were observed. The 16.0% LAC diet provided higher gene expression of transforming-β1 growth factor. Feeding 20.0% CP resulted in better performance than 24.0% CP. The 12.0% LAC diet promoted greater genetic expression of occludin and zonula occludens. Including 12.0% LAC in the diet may improve intestinal epithelial morphology and integrity, and these improvements are more evident when piglets are fed diets with 24.0% CP., (© 2020 Japanese Society of Animal Science.)

Published
2020
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6. Dietary L-arginine supplementation during early gestation of gilts affects conceptuses development.

Author

Costa KA, Saraiva A, Guimarães JD, Marques DBD, Machado-Neves M, Barbosa LMR, Villadiego FAC, Veroneze R, Oliveira LF, Garcia IS, Teixeira SA, and Guimarães SEF

Subjects
Animals, Apoptosis drug effects, Apoptosis genetics, Arginine administration & dosage, Gene Expression Profiling, Litter Size drug effects, Phenotype, Time Factors, Arginine therapeutic use, Dietary Supplements, Fetal Development drug effects, Swine growth & development
Abstract

l-arginine supplementation of sows has led to improvement of reproductive performance, but the mechanisms responsible for the positive effects of arginine during gestation on conceptuses survival and development are still poorly understood. Thus, we aimed to evaluate effects of 1.0% l-arginine supplementation (ARG) on phenotypic traits of commercial gilts, embryos and fetuses, concentration of gilts' blood metabolites, expression of developmental and cellular apoptosis genes in conceptuses of 25 and 35 days. At 25 days, IGF1 gene was more expressed in embryos from ARG than in embryos from control gilts (CON) (P = 0.05). At this same gestational age, ARG embryos tended to be heavier compared to CON (P = 0.07) and ARG gilts showed a trend to have a greater arginine concentration in blood plasma (P = 0.06). However, at 35 days of gestation, arginine concentration in blood plasma of ARG gilts tended to be lower compared to CON (P = 0.06) and ARG fetuses showed smaller cephalic-caudal length (P = 0.05). These results indicate that duration of supplementation is determinant for arginine effects, not only on the females performance but also on the conceptuses, since supplementation upregulated IGF1 expression at 25 days, in addition to the reduction of cephalic-caudal length of 35-day fetuses., (Copyright © 2019 Elsevier Inc. All rights reserved.)

Published
2019
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7. Immunolocalization of leptin and its receptor in the sheep ovary and in vitro effect of leptin on follicular development and oocyte maturation.

Author

Macedo TJS, Santos JMS, Bezerra MÉS, Menezes VG, Gouveia BB, Barbosa LMR, Lins TLBG, Monte APO, Barberino RS, Batista AM, Barros VRP, Wischral A, Queiroz MAA, Araújo GGL, and Matos MHT

Subjects
Animals, Chromatin metabolism, Female, Glutathione metabolism, In Vitro Oocyte Maturation Techniques, Intracellular Space metabolism, Meiosis, Mitochondria metabolism, Ovarian Follicle cytology, Cell Differentiation, Leptin metabolism, Leptin pharmacology, Oocytes cytology, Oocytes metabolism, Ovarian Follicle growth & development, Receptors, Leptin metabolism, Sheep metabolism
Abstract

The aims of the study were to characterize leptin and it is receptor (LEPR) proteins immunoexpression in ovine ovaries and to evaluate the effects of leptin on development of secondary follicles cultured in vitro. The ovaries were collected and fixed for immunohistochemical analysis. Additional pairs of ovaries were collected and secondary follicles were isolated and cultured, for 18 days, in α-MEM + alone or supplemented with 10 or 25 ng/mL of leptin. The antrum formation and fully grown oocytes rates were higher in 25 ng/mL leptin than all treatments. GSH levels and mitochondrial activity were higher in 10 or 25 ng/mL leptin than α-MEM + . 25 ng/mL leptin showed a higher percentage of MII than the α-MEM + . In conclusion, leptin and its receptor are expressed in ovine ovaries and 25 ng/mL leptin promoted higher in vitro maturation rates by improving follicular development, GSH levels and mitochondrial activity of ovine oocytes compared to control medium., (Copyright © 2019 Elsevier B.V. All rights reserved.)

Published
2019
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8. Effect of red propolis extract isolated or encapsulated in nanoparticles on the in vitro culture of sheep preantral follicle: Impacts on antrum formation, mitochondrial activity and glutathione levels.

Author

Nascimento TS, Silva ISM, Alves MCMA, Gouveia BB, Barbosa LMR, Macedo TJS, Santos JMS, Monte APO, Matos MHT, Padilha FF, and Lima-Verde IB

Subjects
Animals, Female, Glutathione analysis, Mitochondria physiology, Ovarian Follicle physiology, Oxidative Stress, Propolis administration & dosage, Propolis chemistry, Sheep, Domestic, Cell Culture Techniques veterinary, Culture Media chemistry, Nanoparticles administration & dosage, Ovarian Follicle drug effects, Propolis pharmacology
Abstract

This study aimed to characterize the hydroethanolic extract of red propolis (HERP) and nanoparticles containing HERP for using as an additive in the culture medium of isolated ovine preantral follicles. HERP was characterized by high-performance liquid chromatography (HPLC) and determination of flavonoid content, and the nanoparticles by the mean particle diameter, polydispersity index (PI) and encapsulation efficiency (EE). The effect of HERP (10 and 20 ηg/ml-HERP10 and HERP20 groups) and nanoparticles (NP10 and NP20 groups) on isolated secondary follicles cultured in vitro for 12 days was observed by morphological evaluation, oxidative stress markers (reactive oxygen species-ROS and glutathione-GSH) and active mitochondria. HPLC showed formononetin as the major compound in the HERP (63.92 ± 0.21 μg/mL). The content of flavonoids ranged from 2.14% to 11.00%. The nanoparticles showed mean diameter of 287.5 ± 3.9 and 479 ± 18.1 ηm; PI of 0.117 ± 0.018 and 0.316 ± 0.039; and EE of 67.15% and 41%, respectively, for the NP10 and NP20 groups. After 12 days of culture, HERP20 and NP20 increased (p < 0.05) the percentage of normal follicles compared to NP10. HERP20 showed significantly higher percentages of antrum formation than control medium (MEM) and NP10 (p < 0.05). HERP20 also showed an increase (p < 0.05) in mitochondrial activity compared to the other treatments, except NP20 (p > 0.05), and increased GSH levels (p < 0.05) compared to MEM and HERP10. In conclusion, use of HERP (20 ηg/ml) on in vitro culture of isolated ovine preantral follicles can increase antrum formation, mitochondrial activity and GSH levels., (© 2018 Blackwell Verlag GmbH.)

Published
2019
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