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Abstract Background In several European Countries, by the end of 2012, CLSI guidelines will be replaced by EUCAST. We compared antimicrobial susceptibility results of a large number of respiratory pathogens using both EUCAST and previously adopted CLSI criteria to evaluate the impact on susceptibility patterns and the possible consequences that could occur in clinical practice due to this replacement. For S. pyogenes and S. aureus, the interpretation of susceptibility data using the EUCAST criteria did not produce relevant changes in comparison to CLSI. Against S. pneumoniae, more restrictive EUCAST breakpoints could lead to increased benzylpenicillin and/or amoxicillin-clavulanate resistance rates, which in turn could translate in increased dosages of these antibiotics or usage of alternative agents for respiratory tract infections. Against S. pneumoniae, M. catarrhalis and H. influenzae, cefuroxime-axetil and cefaclor produced the most divergent results depending on the breakpoints adopted and these striking differences could lead to the revision of those guidelines suggesting these two cephalosporins as alternatives in the management of upper respiratory tract infections. Discussion Many differences exist between CLSI and EUCAST breakpoints. However, only in a few cases do these differences translate in major interpretive category discrepancies. In countries adopting more restrictive EUCAST breakpoints, clinicians should be aware of these discrepancies and that they could be faced with antibiotic-resistant respiratory pathogens more frequently than before. Summary The interpretive discrepancies between EUCAST and CLSI suggest that the discussion on the management of community-acquired respiratory tract infections is still open and further studies are desirable to better define the role of some antibiotics.

Abstract A method is described that allows an accurate mapping of 3' ends of RNAs. In this method a labeled DNA probe, containing the presumed 3' end of the RNA under analysis is allowed to anneals to the RNA itself. Mung-bean nuclease is then used to digest single strands of both RNA and DNA. Electrophoretic fractionation of "protected" undigested, labeled DNA is than performed using a sequence reaction of a known DNA as length marker. This procedure was applied to the analysis of both a polyA RNA (Interleukin 10 mRNA) and non polyA RNAs (sea urchin 18S and 26S rRNAs). This method might be potentially relevant for the evaluation of the role of posttrascriptional control of IL-10 in the pathogenesis of the immune and inflammatory mediated diseases associated to ageing. This might allow to develop new strategies to approach to the diagnosis and therapy of age related diseases.

Abstract An inexpensive Plexiglas apparatus which allows a simple and rapid preparation of horizontal polyacrylamide gels of different dimensions for different purposes, is described. Preparation of such gels is as easy and rapid as agarose gel preparation, and polymerized polyacrylamide gels are used to fractionate proteins or small DNA fragments using a common horizontal electrophoretic tank. This apparatus was used to electrophoretically fractionate proteins or DNA for immuno-blot analyses, particularirly in the study of the allergenic response to Parietaria judaica pollen in senescence, for Southern-blot hybridizations and in the study of DNA polymorphisms.

Objective: To determine the effects of aerobic training in randomized controlled clinical trials on functional capacity, motor symptoms, and oxygen consumption in individuals with Parkinson disease (PD) through a systematic literature review and meta-analysis., Data Sources: PUBMED, Web of Science, CINAHL, SciELO, and Medline databases were searched to identify published studies until September 2023., Study Selection: Randomized controlled clinical trials that evaluated the long-term effect of aerobic exercise in individuals with PD were included., Data Extraction: Two independent reviewers extracted the data and assessed the risk of bias and the Grading of Recommendation Assessment, Development, and Evaluation. In case of disagreement, a third reviewer was consulted., Data Synthesis: Thirteen studies were included in the systematic review, and the number of participants was 588 with an average age of 66.2 years (57-73y). The study's exercise intervention lasted between 6 and 70 weeks, with most studies lasting 10-12 weeks, with 3 sessions per week and an average duration of 47 minutes per session. The meta-analysis revealed that aerobic exercise is effective in enhancing maximal oxygen uptake (standardized mean difference, SMD 0.42 [95% CI, 0.18, 0.66; P=.0007]) and functional capacity (SMD 0.48 [95% CI, 0.24-0.71; P<.0001]). In addition, aerobic exercise can reduce the motor-unified Parkinson disease rating scale (mean difference-2.48 [95% CI, -3.16 to -1.81; P<.00001]) score in individuals with PD., Conclusions: Aerobic exercise training conducted 2-3 times a week, with different intensities (low to high), can be an effective intervention for enhancing functional capacity, maximizing oxygen uptake, and reducing the UPDRS scores in individuals with PD., (Copyright © 2024 American Congress of Rehabilitation Medicine. Published by Elsevier Inc. All rights reserved.)

Highways, the lifeline of the Brazilian economy, transport approximately 75% of the country's economic activity, highlighting its importance. However, professional drivers, accustomed to long daily journeys, make use of tablets widely available in Gas Station, which are known as "Rebites," which could contain a mixture of legal and illegal compounds. Thus, this study aims at the chemical characterization of these through different analytical methods. Initially, we performed a comprehensive screening of compounds present in seven samples collected across the country using high-resolution mass spectrometry (HRMS). The findings revealed caffeine as the main compound, alongside theophylline, lidocaine, and clobenzorex, among others. In the next step, we employ quantitative nuclear magnetic resonance (qNMR) to quantify the caffeine content in the tablets. The results indicated a caffeine concentration ranging between 14% and 31% (m/m), which may imply a daily overdose of this compound from around four tablets. In summary, this investigation provides a chemical characterization of real samples of "Rebites" freely obtained along Brazilian highways. Caffeine emerged as the predominant active compound, with its concentration determined by qNMR analysis. The notable presence of caffeine, combined with other stimulants, depressants, and hallucinogens, underscores the need for strict quality control measures regarding "Rebites" to safeguard public health., (© 2024 American Academy of Forensic Sciences.)

Aerobic exercise with the correct intensity can attenuate motor and non-motor symptoms of Parkinson's disease (PD) and improve the quality of life. However, a specific, validated, non-invasive, and outside the laboratory protocol that assesses physiological variables to prescribe optimal aerobic exercise intensity for people with PD is nonexistent. Therefore, this study aimed to propose a protocol, the Parkinson's critical heart rate test (Parkinson-CHR test), to determine the critical heart rate (CHR) in individuals with PD and verify its validity, reliability, and sensitivity. Fifteen people with idiopathic PD, who were able to practice exercises, were recruited to participate in the study (71.1 ± 6.6 years). The study consisted of two experiments: i) the first one aimed to assess the validity and reliability of the protocol, with participants performing the test twice at a one-week interval; ii) the second experiment aimed to investigate the protocol sensitivity, with individuals being evaluated before and after an 8-week training program according to Parkinson-CHR intensity. In experiment 1, no differences between test and retest were observed in the time to cover the distances (400, 800 and 1200 m), the total heart rate, the critical heart rate, and critical speed (p > 0.05). In experiment 2, there was a reduction in time to cover 400 and 800 m as well as in the total heart rate for all distances after the 8-week training program. The Parkinson-CHR test is a reliable, reproducible, inexpensive, and non-invasive protocol to assess, prescribe, and monitor aerobic exercise intensity in people with PD., Competing Interests: The authors declare no conflict of interest., (Copyright: © Academy of Physical Education in Katowice.)

This study is the first to apply training impulse (TRIMP) and Training Monotony (TM) methodologies, within the realm of sport science, in animal model studies. Rats were divided into Sedentary (SED, n=10) and Training (TR, n=13). TR performed a four-week moderate-intensity interval training with load progression. Lactate kinetics, lactate training impulse (TRIMPLac), maximal speed training impulse (TRIMPSmax) and TM were utilized to develop and monitor training protocol. TR showed an 11.9% increase in time to exhaustion at the second maximum incremental test and a 17.5% increase at the third test. External work was increased by 17.8% at the second test and 30.3% at the third. There was a 10.6% increase in external work at the third test compared to the second for TR. No difference in TRIMPLac between the 1st week (94±9 A.U) and 3rdweek (83±10 A.U) were seen. TRIMPSmax was 2400 A.U. in the 1st week, 2760 A.U. in the 2nd and 3rd weeks, and 3120 A.U. in the 4th week. The TM remained at 1.24 A.U throughout the protocol and there was no dropouts. TRIMPLac and TRIMPSmax contributed to the development and monitoring loads, demonstrating their potential to improve the accuracy of training protocols in animal model research.

The total anaerobic contribution (AC [La-]+PCr ) is a valid and reliable methodology. However, the active muscle mass plays an important role in the AC [La-]+PCr determination, which might influence its reliability. Thus, this study aimed to investigate the effects of two exhaustive intensities on the reliability of the AC [La-]+PCr during a one-legged knee extension (1L-KE) exercise. Thirteen physically active males were submitted to a graded exercise to determine the peak power output (PPO) in the 1L-KE. Then, two constant-load exercises were conducted to task failure at 100% (TTF 100 ) and 110% (TTF 110 ) of PPO, and the exercises were repeated on a third day. The blood lactate accumulation and the oxygen uptake after exercise were used to estimate the anaerobic lactic and alactic contributions, respectively. Higher values of AC [La-]+PCr were found after the TTF 100 compared to TTF 110 (p=0.042). In addition, no significant differences (p=0.432), low systematic error (80.9 mL), and a significant ICC (0.71; p=0.004) were found for AC [La-]+PCr in the TTF 100 . However, an elevated coefficient of variation was found (13.7%). In conclusion, we suggest the use of the exhaustive efforts performed at 100% of the PPO with the 1L-KE model, but its elevated individual variability must be carefully considered in future studies., Competing Interests: The authors declare that they have no conflict of interest., (Thieme. All rights reserved.)

This study aimed to test the reproducibility of the 3-min all-out effort applied using shuttle running and compare its values to aerobic parameters. On the first day, 14 futsal players underwent an exhaustive test to determine the maximal incremental speed (MIS) and anaerobic threshold (AnT). On the second day, the participants performed the 3-min all-out effort (n=14), which was repeated after 48 h (third day) to test its reproducibility (n=11). Peak oxygen consumption (V̇ O 2PEAK ) and peak blood lactate concentrations ([La-]) were determined from 3-min all-out efforts performed through a 20-m shuttle run on the official court. The distance covered, mean speed, and critical speed (CS) during the 3-min all-out presented direct relationships with aerobic parameters determined through the incremental test (r>0.62). The distance covered above CS (D') presented a direct relationship with peak lactate concentrations induced by a 3-min all-out effort (r=0.81). Despite the acceptable levels of reproducibility observed for most of the 3-min all-out variables, the minimal detectable change for D' was high (72%). Our results demonstrated the potential use of mean speed to evaluate aerobic fitness. However, the applicability of the 3-min all-out shuttle run test to monitor training adaptations should be avoided, at least in nonexperienced athletes., Competing Interests: The authors declare that they have no conflict of interest., (Thieme. All rights reserved.)

Introduction: Wheelchair fencing (WF) is a very practiced sport within the Paralympic world. A careful analysis of the characteristics and data of a WF athlete can be useful for coaches and movement experts to better plan a training program. The objective of the study was to evaluate physical fitness assessment methods adopted in WF and to propose a standard operating procedures (SOP)., Evidence Acquisition: Original articles written in English were included in this review, and the population studied was composed of athletes who practiced WF. Studies were searched from PubMed, Web of Science, and Scopus databases using keywords and Boolean operators. Eight studies were included in this review., Evidence Synthesis: Most of the studies converge and agree on the physical, physiological, and technical characteristics of a WF athlete., Conclusions: The scientific literature referring to this sport is very scarce, hence the need for new original studies to optimize the SOP proposed.

This study aims to analyze the agreement and precision between heart rate variability thresholds (HRVT1/2) with ventilatory and lactate thresholds 1 and 2 (VT1/2 and LT1/2) on a treadmill. Thirty-four male students were recruited. Day 1 consisted of conducting a health survey, anthropometrics, and Cardiopulmonary Exercise Test (CPx). On Day 2, after 48 h, a second incremental test was performed, the Cardiopulmonary Stepwise Exercise Test consisting of 3 min stages (CPxS), to determine VT1/2, LT1/2, and HRVT1/2. One-way repeated-measures ANOVA and effect size (η p 2 ) were used, followed by Sidak's post hoc. The Coefficient of Variation (CV) and Typical Error (TE) were applied to verify the precision. Bland Altman and the Intraclass Correlation Coefficient (ICC) were applied to confirm the agreement. HRVT1 showed different values compared to LT1 (lactate, RER, and R-R interval) and VT1 (V̇E, RER, V̇CO 2 , and HR). No differences were found in threshold 2 (T2) between LT2, VT2, and HRVT2. No difference was found in speed and V̇O 2 for T1 and T2. The precision was low to T1 (CV > 12% and TE > 10%) and good to T2 (CV < 12% and TE < 10%). The agreement was good to fair in threshold 1 (VT1, LT1, HRVT1) and excellent to good in T2 (VT1, LT1, HRVT1). HRVT1 is not a valid method (low precision) when using this protocol to estimate LT1 and VT1. However, HRVT2 is a valid and noninvasive method that can estimate LT2 and VT2, showing good agreement and precision in healthy adults.

The purpose of this study was to investigate the effects of three simulated goalball games on neuromuscular, physiological, perceptual, and technical parameters. Ten male players underwent assessments before and immediately after each game. Heart rate was recorded at rest and during all games that were entirely filmed for further technical performance analysis. Exercise significantly decreased knee extensor muscles peak force and percentage of voluntary activation after the second and third games, indicating the presence of central fatigue. Heart rate responses remained predominantly in a range equivalent to moderate activity intensity in all games. In addition, perceptual parameters were associated with reduced frequency of throws and density of actions. These findings suggest significant implications for the management of physical training, game strategy during a competition, and fixture change from three to two games per day.

Background: This study investigated the possible influence of the gender on the responses of swimmers during a taper period (TP)., Methods: Ten males (19±3 years and 73.5±7.8 kg) and ten females (17±2 years and 54.7±7.2 kg) swimmers were submitted to a 12-week training, followed by three weeks of the TP. Before and after the TP we evaluated the performance at 100 m freestyle, stroke parameters and lactacidemic responses; lactate minimum intensity (LMI) and stroke parameters associated with LMI and the propulsive force in tethered swimming. TP consisted of 14 sessions with mean volume 2253±1213 m/session at an intensity below than the LMI, 1730±327 m/session at an intensity near the LMI and 1530±1019 m/session at an intensity above the LMI., Results: Significant effects of the genders were observed for LMI and stroke parameters (P<0.001 and η 2 >0.52 [large]) and propulsive force (P=0.001; η 2 =0.59 [large]). However, no significant effects of the TP were identified in the performance of the 100 m freestyle (P=0.66; η 2 =0.006 [small]), propulsive force (P>0.63; η 2 <0.006 [small]), aerobic parameters (LMI: P=0.32 and η 2 =0.03 [small]) and mechanical parameters (P>0.23; η 2 =0.01 [small]). Nonetheless, the peak blood lactate concentrations were improved after TP (P=0.014; η 2 =0.16 [large]), without significant interactions (P=0.38; η 2 =0.02 [small]), as well as the mechanical parameters during maximum 100 m freestyle (P<0.04 and η 2 >0.10 [medium])., Conclusions: Hence, men and women presenting significantly different values in the age group studied, the responses observed after the TP investigated were the same independent of gender.

Background: Backward extrapolation technique (BE) was used to estimate V̇O2 from postexercise measuring, eliminating oronasal mask (OM) during the efforts. Despite its advantage, literature presents discrepancy in applied methods. Thus, the first aim of this study was to compare different mathematical criteria to estimate values of V̇O2 during a supramaximal effort (V̇O2PEAK), while the second aim was to verify the effects of OM on cycling performance., Methods: Twenty-four male cyclists (35±6 years, 81.3±8.9 kg, 180±6 cm) performed three days of tests, with at least 24 h of interval between each test. Firstly, a graded exercise test was applied to determine V̇O2max and your correspondent intensity (MAP). The second and the third day were destined to supramaximal efforts at 120% of MAP, performed with (Supramask) and without (Suprabe) oronasal mask (OM) in a randomized order. After Suprabe, OM was coupled, and BE was applied. Sixty-six values of V̇O2 were obtained based on a linear regression fitting., Results: V̇O2peak can be estimated using different curve lengths. However, only curves between 20 and 60 s with extrapolation to 3 s or lesser shows at least one consistent criterion. The 60 s curve extrapoled to -3 s was the most accurate criteria (P=0.723; ES=-0.055; r=0.824; Bias=-0.36 and LoA=7.72 mL.kg.min -1 ). Performance was not impaired with OM and was similar in both condition (P=0.84, ES=0.04)., Conclusions: We conclude that it was possible to accurately estimate V̇O2 values of a supramaximal effort without any respiratory apparatus with a time-efficient analysis. Therefore, we recommended the use of a 60 seconds V̇O2 curve analysis with a negative extrapolation for 3 seconds.

The aim of this study was to investigate the effects of ankle and hip muscle fatigue on motor adjustments (experiment 1) and symmetry (experiment 2) of postural control during a quiet standing task. Twenty-three young adults performed a bipedal postural task on separate force platforms, before and after a bilateral ankle and hip muscle fatigue protocol (randomized). Ankle and hip muscles were fatigued separately using a standing calf raise protocol (ankle fatigue) on a step and flexion and extension of the hip (hip fatigue) sitting on a chair, at a controlled movement frequency (0.5Hz), respectively. In both experiments, force, center of pressure, and electromyography parameters were measured. The symmetry index was used in experiment 2 to analyze the postural asymmetry in the parameters. Our main findings showed that muscle fatigue impaired postural stability, regardless of the fatigued muscle region (i.e., ankle or hip). In addition, young adults used an ankle motor strategy (experiment 1) before and after both the ankle and hip muscle fatigue protocols. Moreover, we found increased asymmetry between the lower limbs (experiment 2) during the quiet standing task after muscle fatigue. Thus, we can conclude that the postural motor strategy is not muscle fatigue joint-dependent and a fatigue task increases postural asymmetry, regardless of the fatigued region (hip or ankle). These findings could be applied in sports training and rehabilitation programs with the objective of reducing the fatigue effects on asymmetry and improving balance., Competing Interests: The authors have declared that no competing interests exist.

Friction is a ubiquitous manifestation of nature, and when it is studied at the nanoscale, complex and interesting effects arise from fundamental physical and chemical surface properties. Surprisingly, and probably due to the complexity of nanofriction studies, this aspect has not been completely discussed in prior studies. To fully consider the physicochemical influence in nanoscale friction, amorphous carbon films with different amounts of hydrogen and fluorine were prepared, chemically characterized, and evaluated via lateral force microscopy. Hydrogen and fluorine were selected because although they exhibit different physicochemical properties, both contribute to frictional force reduction. Indeed, to explain the experimental behavior, it is necessary to propose a new damping constant unifying both polarizability (physical) and electronegativity (chemical) properties. The satisfactory agreement between theory and experiments may encourage and enhance deeper discussion and new experiments that take into account the chemical peculiarities of frictional behavior relating to nanoscale elastic regimes.

Physiological responses in futsal have not been studied together with temporal information about the players' stay on the court. The aim of this study was to compare heart rate (HR) and blood lactate concentration ([La - ]) responses between 1-H and 2-H considering the time of permanency of the players on the court at each substitution in a futsal match. HR was recorded during entire match and [La - ] was analyzed after each substitution of seven players. %HR mean (89.61 ± 2.31 vs. 88.03 ± 4.98 %HR max ) and [La - ] mean (8.46 ± 3.01 vs. 8.17 ± 2.91 mmol·L -1 ) did not differ between 1-H and 2-H (ES, trivial-small). Time in intensity zones of 50-100 %HR max differed only in 60-70 %HR max (ES, moderate). HR coefficient of variation throughout the match was low (7%) and among the four outfield players on the court (quartets, 5%). Substitutions (2 player's participation in each half), time of permanence on the court (7.15 ± 2.39 vs. 9.49 ± 3.80 min), ratio between time in- and out-ratio on the court (In:Outcourt = 1:1.30 ± 1:0.48 vs. 1:1.05 ± 1:0.55 min) also were similar between 1-H and 2-H (ES, moderate and small, respectively). Balancing the number of substitutions, and the In:Outcourt ratio of players in both halves of the match, playing lower time at 1-H, ~8 min for each participation in the match, made it possible to maintain intensity of the match in 2-H similar to the 1H. These results are a good guidance to coaches and for application in future studies., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2020 Dos-Santos, da Silva, da Silva Junior, Barbieri, Penafiel, da Silva, Milioni, Vieira, Coledam, Santiago and Papoti.)

This study aimed to propose a conditioning activity (CA) model to stimulate improvement on neuromuscular responses, mechanical parameters and for the 50-m freestyle swimming. Thirteen male swimmers (19 ± 3 years and performances of 77% in relation to World Championship records) performed four CA protocols followed by a maximum performance in the 50-m freestyle. In the first protocol (P1) swimmers performed a standard warm-up (∼15 min); in the second protocol (P2) lunges (3 × 85% of the one-repetition maximum); in the third (P3) pull-ups (3 maximum repetitions) and box jumps 40 cm high and 60 cm deep (1 × 5 with 10% of the corporal weight); and in the fourth protocol (P4) a combination of exercises from the second and third protocols. CA protocols had no effect on the standard warm-up. However, P2 performance (27.01 ± 1.25 s) was similar to P1 (27.01 ± 1.18 s) and presented higher positive effects in mechanical parameters for the swim start performance in comparison to other protocols, contributing to improvements in the 50-m freestyle. In addition, turnaround time also had a negative effect, mainly in P3 (3.12 ± 0.28 s), signaling the improvement of this variable in all protocols (P1: 3.30 ± 0.38 s; P2: 3.17 ± 0.30 s; P4: 3.17 ± 0.34 s). P2 (after: 80 ± 11%; before: 82.7 ± 9.9%) and P3 (after: 82.7 ± 9.9%; before: 85.1 ± 9.7%) presented a possible positive effect on the percentage of voluntary activation in relation to P1 (after: 79.3 ± 10.7%; before: 76.3 ± 12%). In conclusion, the proposed conditioning activity protocols were not efficient for performance improvement in the 50-m freestyle compared to the standard model and seem to specifically influence each phase of the event., (Copyright © 2020 de Arruda, Barbieri, de Andrade, Cursiol, Kalva-Filho, Bertucci and Papoti.)

Ultrasound-assisted approach was successfully applied for the synthesis of mayenite from calcium and aluminum hydroxides and then subsequently impregnated with Ni by the wet impregnation method. The synthesis was performed with a 13 mm probe-type ultrasound, operating under an acoustic power of 30.5 W and a frequency of 20 kHz. Ultrasound application was studied in detail from a 3 k experimental design, where the variables studied were ultrasound time (10-50 min) and calcination temperature (900-1200 °C). Ultrasound promoted an effective dispersion of the precursors in a short time of 10 min leading to a high conversion to mayenite after calcination at 1200 °C. Ultrasound treatment also had a positive effect on Ni impregnation, increasing the dispersion of the metal in the support and leading to a stronger interaction of nickel-containing species with mayenite support. The use of ultrasound application has proved to be attractive both for catalyst properties and for facilitating catalyst synthesis., (Copyright © 2020 Elsevier B.V. All rights reserved.)

Background: Investigations of β-alanine supplementation shows effects on metabolic (aerobic and anaerobic) participation and performance on swimming by a possible blood acidosis buffering. Considering this background, the objective of the present study was to analyze the effects of β-alanine supplementation on metabolic contribution and performance during 400-m swim., Methods: Thirteen competitive swimmers underwent a 6-week, double-blind placebo-controlled study, ingesting 4.8 g.day - 1 of β-alanine or placebo. Before and after the supplementation period, the total anaerobic contribution (TAn) and 30-s all-out tethered swimming effort (30TS) were assessed. Anaerobic alactic (AnAl) and lactic energy (AnLa) was assumed as the fast component of excess post-exercise oxygen consumption and net blood lactate accumulation during exercise (∆[La - ]), respectively. Aerobic contribution (Aer) was determined by the difference between total energy demand and TAn. In addition to conventional statistical analysis (Repeated measures ANOVA; p > 0.05), a Bayesian repeated measures ANOVA was used to evidence the effect probability (BF incl )., Results: No differences and effects were found between groups, indicating no supplementation effects. Repeated measures ANOVA, with confirmation of effect, was indicate reduce in ∆Lactate (p: 0.001; BF incl : 25.02); absolute AnLa (p: 0.002; BF incl : 12.61), fatigue index (p > 0.001; BF incl : 63.25) and total anaerobic participation (p: 0.008; BF incl : 4.89)., Conclusions: Thus, the results demonstrated that all changes presented were evidenced as a result of exposure to the training period and β-alanine supplementation doesn't affect metabolic contribution and performance during 400-m freestyle.

Background: Task constraints and players' asymmetry influences on lower extremity (i.e. kicking limb) kinematics during futsal instep kicking. However, support limb behavior when shooting in a futsal context was not previously investigated, and its potential role on such discrepant motor outputs is still unclear. Thus, the study aimed to compare kinematic features of the support limb and approach run between kicking a stationary and a rolling ball using dominant and non-dominant limbs., Methods: Ten futsal players participated (21.88±2.86 years-old, 73.66±4.17 kg and 1.75±0.04 m) and performed kicks (five per limb per condition) with the dominant and non-dominant limbs in stationary and rolling ball conditions. Kinematic analysis comprised determination of support limb angular joint (hip, knee and ankle) displacement and velocity, approach run distance, angle, linear velocity, step length and width, support foot-to-ball distance, ball velocity (120 Hz) and accuracy (60 Hz)., Results: Hip adjustments (greater extension) in the support limb when kicking a rolling ball contributed in maintaining similar performance (e.g., ball velocity) to kicking a stationary ball, compensating for the lower approach run velocity and longer support foot to ball distance. Kicking with the non-dominant limb demonstrated a lower approach run velocity and the non-dominant support limb presented different angular motion compared to the dominant support limb in hip (< internal rotation), knee (< flexion), and ankle joints (< plantar flexion), being harmful to performance in both kicking stationary and rolling balls., Conclusions: Kicking a stationary and rolling ball presented similar performance, but compared to the dominant side, futsal instep kicks performed with the non-dominant support limb induces lower approach run velocity and inefficient angular joint motion, either harmful to performance output.

Ankle muscle fatigue has been shown to increase body sway. In addition, body sway in quiet upright standing is reduced when saccadic eye movements are performed. The purpose of this study was to investigate the effects of visual information manipulation on postural control during ankle muscle fatigue in young adults. Twenty young adults performed: (1) two 60-s trials in quiet bipedal standing with eyes open, eyes closed, and while performing saccadic eye movements; (2) maximum voluntary isometric contractions in a leg press device, custom-made to test ankle plantar flexion force; (3) a calf raise exercise on top of a step to induce ankle muscle fatigue; and (4) a repetition of items 1 and 2. Postural sway parameters were compared with two-way ANOVAs (vision condition × fatigue; p  < 0.05). Ankle muscle fatigue increased anterior-posterior and medial-lateral displacement and RMS of sway, as well as sway area. Saccadic eye movements reduced anterior-posterior displacement and RMS of sway and area of sway compared to eyes open and eyes closed conditions. Both saccadic eye movements and eyes closed increased the frequency of AP sway compared to the eyes open condition. Finally, anterior-posterior displacement, anterior-posterior RMS, and both anterior-posterior and medial-lateral sway frequency were affected by an interaction of fatigue and vision condition. Without muscle fatigue, closing the eyes increased anterior-posterior displacement and RMS of sway, compared to eyes open, while during muscle fatigue closing the eyes closed reduced anterior-posterior displacement and had no significant effect on anterior-posterior RMS. In conclusion, body sway was increased after induction of ankle muscle fatigue. Saccadic eye movements consistently reduced postural sway in fatigued and unfatigued conditions. Surprisingly, closing the eyes increased sway in the unfatigued condition but reduced sway in the fatigued condition.

Purpose: Training methods based on small-sided game (SSG) seem to promote physiological and tactical benefits for soccer players as they present characteristics more specific to the game. Thus, the main objective of the present study was to analyze the hormonal, biochemical, and autonomic parameters in an acute manner and the recovery dynamics (up to 72 h after) in a SSG. Methods: Thirteen professional female soccer players participated in the study (18.8 ± 0.8 years, body mass 59.4 ± 6.2 kg, and height 1.68 ± 0.05 m). During and after the SSG session (4 min × 4 min separated by 3 min of passive interval and 120 m 2 coverage per player), autonomic modulation was analyzed in the time and frequency domains using heart rate variability, and blood samples (5 ml) were collected before (0 h) and after (10 min and 24, 48, 72 h) the SSG for biochemical and hormonal analysis. Results: The SSG induced an increase effect for LF (low frequency) (92,52%; Very likely increase ) and a decrease effect for HF (high frequency) values (-65,72%; Very likely decrease ), after 10 min of recovery. The LF/HF increase after 10 min of recovery (386,21%; Very likely increase ). The RMSSD (square root of the mean squared differences of the successive N-N intervals) and pNN50 (measure of the number of adjacent NN intervals which differ by more than 50 ms) values presented a decrease effect 10 min after SSG (61,38%; Very likely decrease and -90%; Very likely decrease ). The CK (creatine kinase) values presented no changes 10 min after SSG. The LDH (lactate dehydrogenase) values presented an increase effect 10 min after the SSG (19,22%; Likely increase ). Both testosterone and cortisol concentrations presented the same behavior after SSG, where no alterations were observed with after 10 min (<0,37%; Most likely trivial ). Conclusion: The SSG promoted significant cardiovascular stress that was restored within the first 24 h of recovery. Parasympathetic parameters continued to increase while sympathetic parameters declined significantly during the 72 h of recovery. In addition, the reduced game did not alter biochemical or hormonal responses during the 72 h.

Taurine can affect the energy system metabolism, specifically the lipid metabolism, since an increase in lipid oxidation may promote carbohydrate savings. We hypothesized that taurine supplementation associated with high-intensity exercise could increase levels of lipolysis, benefiting swimmer performance. Nine male competitive swimmers performed two 400-m front crawl maximal efforts with a 1-week washout, and the athletes received 6 g of taurine (TAU) or placebo (PLA) supplementation 120 min before performing the effort. Oxygen consumption and the contribution of the energy systems were analyzed post effort using a Quark CPET gas analyzer. Blood samples were collected before, and 5 min post the effort for taurine and glycerol analysis. Immediately before and 3, 5, and 7 min post the effort, blood samples from the earlobe were collected to determine lactate levels. An increase of 159% was observed in taurine plasma levels 120 min post ingestion. Glycerol levels were higher in both groups post effort; however, the TAU condition promoted an 8% higher increase than the PLA. No changes were observed in swimmer performance or lactate levels; however, the percentage change in lactate levels (∆[La - ]) was different (TAU: 9.36 ± 2.78 mmol L -1 ; PLA: 11.52 ± 2.19 mmol L -1 , p = 0.04). Acute taurine supplementation 120 min before performing a maximal effort did not improve swimmer performance; however, it increased glycerol plasma levels and reduced both the ∆[La - ] and lactic anaerobic system contribution.

Total anaerobic contribution (TAn) can be assessed by accumulated oxygen deficit, and through sum of glycolytic and phosphagen contribution which enable the evaluation of TAn without influences on mechanical parameters. However, little is known about the difference of TAn within swimming distances. Therefore, the objectives of the present study were to determine and compare the TAn in different performances using the backward extrapolation technique and amount of lactate accumulated during exercise, and relate it with swimming performance. Fourteen competitive swimmers performed five maximal front crawl swims of 50, 100, 200, 400, and 800 m. The total phosphagen (AnAl) and glycolytic (AnLa) contributions were assumed as the fast component of post-exercise oxygen consumption (EPOC FAST ) and amount of blood lactate accumulated during exercise, respectively. TAn was the sum of AnAl and AnLa. Significantly lower values of AnLa were observed in the 800 m ( p < 0.01) than other distances. For AnAl, the 50 m performance presented the lowest values, followed by 100 and 800 m ( p < 0.01). The highest values of AnAl were observed in the 200 and 400 m ( p > 0.13). The TAn was significantly higher in the 200 and 400 m performances than observed at 50 and 800 m ( p < 0.01). Anaerobic contributions were correlated with 50, 100, 200, and 400 m performances ( p < 0.01). The AnAl contribution was not correlated with 400 m performance. Anaerobic parameters were not correlated with 800 m performance. In conclusion, the highest values of anaerobic contribution were observed in the 200 and 400 m distances. Moreover, TAn is important to performances below 400 m, and may be used in training routines.

The aims of the present study were to verify the contributions of the energy systems during repeated sprints with a short recovery time and the associations of the time- and power-performance of repeated sprints with energetic contributions and aerobic and anaerobic variables. 13 healthy men performed the running-based anaerobic sprint test (RAST) followed by an incremental protocol for lactate minimum intensity determination. During the RAST, the net energy system was estimated using the oxygen consumption and the blood lactate responses. The relative contributions of oxidative phosphorylation, glycolytic, and phosphagen pathways were 38, 34, and 28%, respectively. The contribution of the oxidative pathway increased significantly during RAST especially from the third sprint, at the same time that power- and time-performances decreases significantly. The phosphagen pathway was associated with power-performance (peak power=432±107 W, r=0.65; mean power=325±80 W, r=0.65; minimum power=241±77 W, r=0.57; force impulse=1 846±478 N·s, r=0.74; p <0.05). The time-performance (total time=37.9±2.5 s; best time=5.7±0.4 s; mean time=6.3±0.4 s; worst time=7.0±0.6 s) was significantly correlated with the oxidative phosphorylation pathway (0.57+0.65; p <0.05) and glycolytic pathway (0.57+<+r>0.58; p <0.05). The oxidative pathway appears to play an important role in better recovery between sprints, and the continued use of the glycolytic metabolic pathway seems to decrease sprint performances. Finally, the phosphagen pathway was linked to power production/maintenance., (© Georg Thieme Verlag KG Stuttgart · New York.)

Purpose: The aim of the present study was to investigate the influence of futsal match-related fatigue on running performance, neuromuscular variables, and finishing kick speed and accuracy. Methods: Ten professional futsal players participated in the study (age: 22.2 ± 2.5 years) and initially performed an incremental protocol to determine maximum oxygen uptake ([Formula: see text]: 50.6 ± 4.9 mL.kg -1 .min -1 ). Next, simulated games were performed, in four periods of 10 min during which heart rate and blood lactate concentration were monitored. The entire games were video recorded for subsequent automatic tracking. Before and immediately after the simulated game, neuromuscular function was measured by maximal isometric force of knee extension, voluntary activation using twitch interpolation technique, and electromyographic activity. Before, at half time, and immediately after the simulated game, the athletes also performed a set of finishing kicks for ball speed and accuracy measurements. Results: Total distance covered (1st half: 1986.6 ± 74.4 m; 2nd half: 1856.0 ± 129.7 m, P = 0.00) and distance covered per minute (1st half: 103.2 ± 4.4 m.min -1 ; 2nd half: 96.4 ± 7.5 m.min -1 , P = 0.00) demonstrated significant declines during the simulated game, as well as maximal isometric force of knee extension (Before: 840.2 ± 66.2 N; After: 751.6 ± 114.3 N, P = 0.04) and voluntary activation (Before: 85.9 ± 7.5%; After: 74.1 ± 12.3%, P = 0.04), however ball speed and accuracy during the finishing kicks were not significantly affected. Conclusion: Therefore, we conclude that despite the decline in running performance and neuromuscular variables presenting an important manifestation of central fatigue, this condition apparently does not affect the speed and accuracy of finishing kicks.

The assessment of aerobic endurance is important for training prescription in soccer, and is usually measured by straight running without the ball on a track or treadmill. Due to the ball control and technical demands during a specific soccer test, the running speeds are likely to be lower compared to a continuous incremental test. The aim of the present study was to compare the heart rate (HR), rating of perceived exertion (RPE) and speeds corresponding to 2.0 mmol∙L(-1), 3.5 mmol∙L(-1), lactate threshold (Dmax method) and peak lactate determined in the laboratory and in the Hoff circuit soccer-specific test. Sixteen soccer players (16±1 years) underwent two incremental tests (laboratory and Hoff circuit tests). The speeds were significantly higher in the treadmill test than on the Hoff circuit (2.0 mmol∙L(-1): 9.5±1.2 and 8.1±1.0 km∙h(-1); 3.5 mmol∙L(-1): 12.0±1.2 and 10.2±1.1 km∙h(-1); Dmax: 11.4±1.4 and 9.3±0.4 km∙h(-1); peak lactate: 14.9±1.6 and 10.9±0.8 km∙h(-1)). The HR corresponding to 3.5 mmol∙L-1 was significantly higher on the Hoff circuit compared to the laboratory test (187.5±18.0 and 178.2±17.6 bpm, respectively; P <0.001), while the RPE at the last incremental stage was lower on the Hoff circuit (P < 0.01). The speeds during the Hoff specific soccer test and the HR corresponding to 2.0 mmol∙L(-1), 3.5 mmol∙L(-1) and Dmax/threshold were different compared with the laboratory test. The present study shows that it is possible to assess submaximal endurance related variables specifically in soccer players.

The influence of cardiorespiratory fitness (VO2max) on anthropometric variables and PPARG mRNA expression was investigated. Monozygotic twin pairs aged 11-18 years were grouped into discordant (D) and concordant (C) high and low VO2max groups. VO2max was determined by progressive maximal exercise test on treadmill with gas exchange analysis. Body mass (BM), BMI, waist circumference (WC), triceps (TR), and subscapular (SB) skinfold thicknesses were measured. Twins from the discordant group had differences in VO2max values (D-high = 45.9 ± 10.0 versus D-low = 32.4 ± 10.6 mL·kg(-1)·min(-1), P = 0.025), while no differences were found in the concordant group (C-high = 42.4 ± 9.2 versus C-low = 38.8 ± 9.8 mL·kg(-1)·min(-1), P = 0.952). In discordant group, VO2max was negatively correlated with TR + SB (r = -0.540, P = 0.021) and positively correlated with PPARG expression in leukocytes (r = 0.952, P = 0.001). Moreover, PPARG expression was directly correlated with BM (r = 0.714, P = 0.047) and height (r = 0.762, P = 0.028). In concordant twins, VO2max was inversely correlated with BM (r = -0.290, P = 0.027), BMI (r = -0.472, P = 0.001), WC (r = -0.426, P = 0.001), and TR + SB (r = -0.739, P = 0.001). Twins D-high had 1.78-fold greater PPARG expression when compared with twins D-low (P = 0.048). In conclusion, the cardiorespiratory fitness may modulate PPARG expression in childhood and adolescence, independently of the genetic background.

Objective: To determine if glucose and insulin concentrations are regulated by cardiorespiratory fitness (VO2max) regardless of their genetic effects., Methods: This cross-sectional study enrolled 38 pairs of young monozygotic twins (11 to 18 years-old). All subjects underwent a progressive maximal exercise test on a treadmill to determine the VO2max with gas exchange analysis (MedGraphics VO2000® - Medical Graphics Corp., St. Paul, MN). Blood samples were drawn after fasting to determine glucose and insulin levels. Monozygosity was confirmed by genotyping 15 informative genetic markers. Nine pairs had at least 10mL.kg-1.min-1 difference in VO2max and were divided into the more and less active group, according to their VO2max. Mean differences between more and less active groups were evaluated by Wilcoxon's test for paired data., Results: On average, twins from the more active group presented a 17% (13.5±3.7mL.kg-1.min-1) higher VO2max compared to their less active siblings. No significant differences were observed between the groups for fasting insulin (36.5±34.6 versus 25.3±13.7mg/dL; p<0.813). However, the more active twins had lower fasting glucose than the less active ones (82.9±7.3 versus 86.7±7.6mg/dL; p<0.010)., Conclusions: In this case-control study (discordant monozygotic twins), the less active co-twins were characterized by higher fasting plasma glucose levels. This implies that poor cardiorespiratory fitness can be associated with defective glucose metabolism regardless of genetic factors.

Background: Fructose-based diets are apparently related to the occurrence of several metabolic dysfunctions, but the effects of the consumption of high amounts of fructose on body tissues have not been well described. The aim of this study was to analyze the general characteristics and the lipid content of different tissues of rats after chronic ingestion of a fructose rich soft drink., Methods: Forty-five Wistar rats were used. The rats were divided into three groups (n = 15) and allowed to consume water (C), light Coca Cola (R) (L) or regular Coca Cola(R) (R) as the sole source of liquids for eight weeks., Results: The R group presented significantly higher daily liquid intake and significantly lower food intake than the C and L groups. Moreover, relative to the C and L groups, the R group showed higher triglyceride concentrations in the serum and liver. However, the L group animals presented lower values of serum triglycerides and cholesterol than controls., Conclusions: Based on the results, it can be concluded that daily ingestion of a large amount of fructose- rich soft drink resulted in unfavorable alterations to the lipid profile of the rats.

In total, 41 consecutive patients with "idiopathic carpal tunnel syndrome" and abnormal electrophysiologic findings who underwent carpal tunnel release were studied prospectively. The focus of this investigation was the evaluation of the levels of specific chemical mediators within the serum and flexor tenosynovium of these patients. Blood was collected from these patients within 1 week prior to carpal tunnel release, and flexor tenosynovium was obtained at time of surgery. Specimens were then analyzed to determine the levels of interleukins 1 and 6, prostaglandin E(2) (PGE(2)), and malondialdehyde bis diethyl acetal. These values were compared to those of controls who had no evidence of carpal tunnel syndrome. A significant increase was noted in the serum malondialdehyde and tenosynovial levels of malondialdehyde, interleukin 6, and prostaglandin PGE(2) compared to controls. The elevated levels of these biologic factors and the absence of interleukin 1 elevation support a noninflammatory ischemia-reperfusion etiology for so-called "idiopathic carpal tunnel syndrome" that causes progressive edema and fibrosis of the tissues within the carpal canal. These findings correlate with previous histopathology reports. We believe that "idiopathic carpal tunnel syndrome" is an "-osis" not an "-itis.", (Copyright 2002 Wiley-Liss, Inc. MICROSURGERY 22:378-385 2002)

Osteomyelitis involving the tubular bones of the hand is a rare and potentially devastating disease. An early and accurate diagnosis combined with aggressive surgical debridement and appropriate antibiotics remains the cornerstone of treatment. This article reviews current principles involving the etiology, pathophysiology, diagnosis, and treatment of osteomyelitis of the hand.

Carpal tunnel release is the most common hand operation performed in this country. In the absence of specific systemic diseases, the etiology and persistence of pain and dysfunction even after surgical decompression is poorly understood. The focus of this investigation was to investigate the biological factors present within the patients serum that may lead to increased sensitivity to pain. Tissue was collected from patients during surgery. The tissue was homogenized and the homogenate analyzed for the presence of IL-1, IL-6, prostaglandin E series (PGE2). The levels were compared with volunteers that had no evidence of carpal tunnel syndrome or pain. The results showed similar levels of IL-1 (range 42-26 ng/ml) in tissue homogenates, and a significant increase in levels of IL-6 and malionaldehyde bis-(diethyl acetal) in CTS patients in comparison to control tissues. This increase may be associated with oxidative changes occurring as a result of ischemia and reperfusion. Tissue homogenates were also evaluated for PGE2. The CTS tissues showed a five fold elevation in PGE2 compared to control tissues. Levels of PGE2 in CTS tissues were statistically different using a two-tailed student T-test. Increased levels of PGE2 can enhance vascular permeability at the site of injury, and can play an important role in activating adenylate cyclase which increases intracellular cyclic adenosine monophosphate (cAMP). This increase in cAMP levels can inhibit functional responses to other inflammatory stimuli. Increases in PGE2 can also cause sensitization of the nerve endings so that a normal stimulus that would not necessarily cause pain will now be experienced as painful. The results of this study demonstrate that arachidonic acid metabolites PGE2 may be responsible for both the pathological changes and clinical symptomatology in carpal tunnel syndrome.