140 results on '"Barbero, Francesco"'
Search Results
2. Changes of physico-chemical properties of nano-biomaterials by digestion fluids affect the physiological properties of epithelial intestinal cells and barrier models
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Antonello, Giulia, Marucco, Arianna, Gazzano, Elena, Kainourgios, Panagiotis, Ravagli, Costanza, Gonzalez-Paredes, Ana, Sprio, Simone, Padín-González, Esperanza, Soliman, Mahmoud G., Beal, David, Barbero, Francesco, Gasco, Paolo, Baldi, Giovanni, Carriere, Marie, Monopoli, Marco P., Charitidis, Costas A., Bergamaschi, Enrico, Fenoglio, Ivana, and Riganti, Chiara
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- 2022
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3. Vorbereitung auf eine Pandemie
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Yan, Xiaowei, additional, Barbero, Francesco, additional, and Wunderlich, Robert, additional
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- 2024
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4. Multifunctional nanoassemblies target bacterial lipopolysaccharides for enhanced antimicrobial DNA delivery
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Montis, Costanza, Joseph, Pierre, Magnani, Chiara, Marín-Menéndez, Alejandro, Barbero, Francesco, Estrada, Amalia Ruiz, Nepravishta, Ridvan, Angulo, Jesus, Checcucci, Alice, Mengoni, Alessio, Morris, Christopher J., and Berti, Debora
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- 2020
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5. Vorbereitung auf eine Pandemie
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Yan, Xiaowei, primary, Barbero, Francesco, primary, and Wunderlich, Robert, additional
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- 2023
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6. A New Paclitaxel Formulation Based on Secretome Isolated from Mesenchymal Stem Cells Shows a Significant Cytotoxic Effect on Osteosarcoma Cell Lines
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Banche Niclot, Alessia Giovanna Santa, primary, Marini, Elena, additional, Ferrero, Ivana, additional, Barbero, Francesco, additional, Rosso, Elena, additional, Fenoglio, Ivana, additional, Barge, Alessandro, additional, Pessina, Augusto, additional, Coccè, Valentina, additional, Paino, Francesca, additional, Mareschi, Katia, additional, and Fagioli, Franca, additional
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- 2023
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7. 144 Occupational Exposure to Particles Measured by Nanoparticles Tracking Analysis (NTA) in Exhaled Breath Condensate (EBC).
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Panizzolo, Marco, primary, Barbero, Francesco, additional, Squillacioti, Giulia, additional, Ghelli, Federica, additional, Bono, Roberto, additional, Bellisario, Valeria, additional, Guseva-Canu, Irina, additional, Fenoglio, Ivana, additional, and Bergamaschi, Enrico, additional
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- 2023
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8. Nanoparticle concentration in Exhaled Breath Condensate as a novel biomarker for assessing exposure to nanomaterials in occupational environment
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Panizzolo, Marco, primary, Barbero, Francesco, additional, Squillacioti, Giulia, additional, Bellisario, Valeria, additional, Ghelli, Federica, additional, Fenoglio, Ivana, additional, Bergamaschi, Enrico, additional, and Bono, Roberto, additional
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- 2023
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9. Nano- and microplastics : a comprehensive review on their exposure routes, translocation, and fate in humans
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Ramsperger, Anja F.R.M., Bergamaschi, Enrico, Panizzolo, Marco, Fenoglio, Ivana, Barbero, Francesco, Peters, Ruud, Undas, Anna, Purker, Sebastian, Giese, Bernd, Lalyer, Carina R., Tamargo, Alba, Moreno-Arribas, M.V., Grossart, Hans Peter, Kühnel, Dana, Dietrich, Jana, Paulsen, Friedrich, Afanou, Anani K., Zienolddiny-Narui, Shan, Eriksen Hammer, Stine, Kringlen Ervik, Torunn, Graff, Pål, Brinchmann, Bendik C., Nordby, Karl Christian, Wallin, Håkan, Nassi, Matteo, Benetti, Federico, Zanella, Michela, Brehm, Julian, Kress, Holger, Löder, Martin G.J., Laforsch, Christian, Ramsperger, Anja F.R.M., Bergamaschi, Enrico, Panizzolo, Marco, Fenoglio, Ivana, Barbero, Francesco, Peters, Ruud, Undas, Anna, Purker, Sebastian, Giese, Bernd, Lalyer, Carina R., Tamargo, Alba, Moreno-Arribas, M.V., Grossart, Hans Peter, Kühnel, Dana, Dietrich, Jana, Paulsen, Friedrich, Afanou, Anani K., Zienolddiny-Narui, Shan, Eriksen Hammer, Stine, Kringlen Ervik, Torunn, Graff, Pål, Brinchmann, Bendik C., Nordby, Karl Christian, Wallin, Håkan, Nassi, Matteo, Benetti, Federico, Zanella, Michela, Brehm, Julian, Kress, Holger, Löder, Martin G.J., and Laforsch, Christian
- Abstract
Contamination of the environment with nano-and microplastic particles (NMPs) and its putative adverse effects on organisms, ecosystems, and human health is gaining increasing scientific and public attention. Various studies show that NMPs occur abundantly within the environment, leading to a high likelihood of human exposure to NMPs. Here, different exposure scenarios can occur. The most notable exposure routes of NMPs into the human body are via the airways and gastrointestinal tract (GIT) through inhalation or ingestion, but also via the skin due to the use of personal care products (PCPs) containing NMPs. Once NMPs have entered the human body, it is possible that they are translocated from the exposed organ to other body compartments. In our review article, we combine the current knowledge on the (1) exposure routes of NMPs to humans with the basic understanding of the potential (2) translocation mechanisms into human tissues and, consequently, their (3) fate within the human body. Regarding the (1) exposure routes, we reviewed the current knowledge on the occurrence of NMPs in food, beverages, personal care products and the air (focusing on indoors and workplaces) and found that the studies suggest an abundant presence of MPs within the exposure scenarios. The overall abundance of MPs in exposure matrices relevant to humans highlights the importance of understanding whether NMPs have the potential for tissue translocation. Therefore, we describe the current knowledge on the potential (2) translocation pathways of NMPs from the skin, GIT and respiratory systems to other body compartments. Here, particular attention was paid to how likely NMPs can translocate from the primary exposed organs to secondary organs due to naturally occurring defence mechanisms against tissue translocation. Based on the current understanding, we conclude that a dermal translocation of NMPs is rather unlikely. In contrast, small MPs and NPs can generally translocate from the GIT and resp
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- 2023
10. Interaction between a cationic bolaamphiphile and DNA: The route towards nanovectors for oligonucleotide antimicrobials
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Mamusa, Marianna, Resta, Claudio, Barbero, Francesco, Carta, Davide, Codoni, Doroty, Hatzixanthis, Kostas, McArthur, Michael, and Berti, Debora
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- 2016
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11. Photoresponsive Inorganic Nanomaterials in Oncology.
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Barbero, Francesco, Gul, Shagufta, Perrone, Guido, and Fenoglio, Ivana
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LITERATURE reviews ,NANOSTRUCTURED materials ,REACTIVE oxygen species ,ONCOLOGY - Abstract
The diagnosis and treatment of cancer are continuously evolving in search of more efficient, safe, and personalized approaches. Therapies based on nanoparticles or physical stimuli-responsive substances have shown great potential to overcome the inherent shortcomings of conventional cancer therapies. In fact, nanoparticles may increase the half-life of chemotherapeutic agents or promote the targeting in cancer tissues while physical stimuli-responsive substances are more effective and safer with respect to traditional chemotherapeutic agents because of the possibility to be switched on only when needed. These 2 approaches can be combined by exploiting the ability of some inorganic nanomaterials to be activated by light, ultrasounds, magnetic fields, or ionizing radiations. Albeit the development of stimuli-responsive materials is still at the early stages, research in this field is rapidly growing since they have important advantages with respect to organic nanoparticles or molecular substances, like higher stability, and higher efficiency in converting the stimulus in heat or, in some cases, reactive oxygen species. On the other hand, the translation process is slowed down by issues related to safety and quality of the formulations. This literature review summarizes the current advancements in this research field, analysing the most promising materials and applications. [ABSTRACT FROM AUTHOR]
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- 2023
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12. Nano- and microplastics: a comprehensive review on their exposure routes, translocation, and fate in humans
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Ramsperger, Anja F.R.M., primary, Bergamaschi, Enrico, additional, Panizzolo, Marco, additional, Fenoglio, Ivana, additional, Barbero, Francesco, additional, Peters, Ruud, additional, Undas, Anna, additional, Purker, Sebastian, additional, Giese, Bernd, additional, Lalyer, Carina R., additional, Tamargo, Alba, additional, Moreno-Arribas, M. Victoria, additional, Grossart, Hans-Peter, additional, Kühnel, Dana, additional, Dietrich, Jana, additional, Paulsen, Friedrich, additional, Afanou, Anani K., additional, Zienolddiny-Narui, Shan, additional, Eriksen Hammer, Stine, additional, Kringlen Ervik, Torunn, additional, Graff, Pål, additional, Brinchmann, Bendik C., additional, Nordby, Karl-Christian, additional, Wallin, Håkan, additional, Nassi, Matteo, additional, Benetti, Federico, additional, Zanella, Michela, additional, Brehm, Julian, additional, Kress, Holger, additional, Löder, Martin G.J., additional, and Laforsch, Christian, additional
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- 2023
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13. Useful Gut Model for Plastic Particles Assessing
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Giulia, Antonello, Esposito, Marianna, Barbero, Francesco, Macaraig Mansilungan, Camille, Fenoglio, Ivana, Riganti, Chiara, and Bergamaschi, Enrico
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microplastics ,toxicity ,cytotoxicity ,Caco-2 ,gastro-intestinal barrier ,HT29-MTX ,nanoplastics ,plastic particles - Abstract
Emerging evidence suggest that human exposure to micro-plastics is rising and it most happened via ingestion and/or inhalation (Wright SL et al. 2017). In fact, plastics particles were detected in various product for human consumption such as seafood, honey, sugar, and drinking water (von Moos et al. 2012; Liebezeit et al. 2013; Koelmans et al. 2019). Once ingested, they can reach the intestinal epithelium. Their biocompatibility or toxic effects on gastro-intestinal barrier is matter of debate, also because of the lack of suitable models of physiological digestion and gastro-intestinal barrier. The simplest model developed is represented by Caco-2 cells cultured on semi-permeable filter supports for 21 days, a time point that make them differentiating into enterocyte-like cells, with transport and permeability features like human intestinal tissue (Lundquist et al 2016). A more complex model is composed of the co-culture of Caco-2 and HT29-MTX cells that to methotrexate (MTX) develop the ability to produce mucus (Gamboa et al. 2013): this model includes the mucus-secreting goblet cells important for the presence of mucus layer in the intestine. To better mimic the intestinal physiology, another cell type (Raji B) can be added to the co-culture to allow Caco-2 differentiation into gut-associated lymphoid tissue microfold cells (M-cells) that can transport micro- and nanoparticles through transcytosis process (Gullberg et al. 2000).
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- 2022
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14. Inhibitory Effect against Listeria monocytogenes of Carbon Nanoparticles Loaded with Copper as Precursors of Food Active Packaging
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Scattareggia Marchese, Adriana, primary, Destro, Elena, additional, Boselli, Carlo, additional, Barbero, Francesco, additional, Malandrino, Mery, additional, Cardeti, Giusy, additional, Fenoglio, Ivana, additional, and Lanni, Luigi, additional
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- 2022
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15. Desorption and transfer processes in different classes of dyes
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Cova, Camilla Maria, primary, Conti, Laura, additional, Barbero, Francesco, additional, Berti, Debora, additional, and Bianchetti, Giulia Ottavia, additional
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- 2022
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16. Vorbereitung auf eine Pandemie.
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Yan, Xiaowei, Barbero, Francesco, and Wunderlich, Robert
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- 2023
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17. Role of Common Cell Culture Media Supplements on Citrate-Stabilized Gold Nanoparticle Protein Corona Formation, Aggregation State, and the Consequent Impact on Cellular Uptake
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Barbero, Francesco, primary, Michelini, Sara, additional, Moriones, Oscar H., additional, Patarroyo, Javier, additional, Rosell, Jordi, additional, F. Gusta, Muriel, additional, Vitali, Michele, additional, Martín, Luna, additional, Canals, Francesc, additional, Duschl, Albert, additional, Horejs-Hoeck, Jutta, additional, Mondragón, Laura, additional, Bastús, Neus G., additional, and Puntes, Víctor, additional
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- 2022
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18. Stressor-dependant changes in immune parameters in the terrestrial isopod crustacean, Porcellio scaber
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Mayall, Craig, Dolar, Andraž, Jemec Kokalj, Anita, Novak, Sara, Razinger, Jaka, Barbero, Francesco, Puntes, Victor, and Drobne, Damjana
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inorganic chemicals ,nanodelci ,spremembe imunskih parametrov ,technology, industry, and agriculture ,respiratory system ,immune response ,cerium nanoparticles ,gold nanoparticles ,mental disorders ,udc:577 ,kopenski raki ,imunski odziv ,woodlice ,health care economics and organizations ,haemocyte - Abstract
We compared the changes of selected immune parameters of Porcellio scaber to different stressors. The animals were either fed for two weeks with Au nanoparticles (NPs), CeO$_2$ NPs, or Au ions or body-injected with Au NPs, CeO$_2$ NPs, or lipopolysaccharide endotoxin. Contrary to expectations, the feeding experiment showed that both NPs caused a significant increase in the total haemocyte count (THC). In contrast, the ion-positive control resulted in a significantly decreased THC. Additionally, changes in phenoloxidase (PO)-like activity, haemocyte viability, and nitric oxide (NO) levels seemed to depend on the stressor. Injection experiments also showed stressor-dependant changes in measured parameters, such as CeO$_2$ NPs and lipopolysaccharide endotoxin (LPS), caused more significant responses than Au NPs. These results show that feeding and injection of NPs caused an immune response and that the response differed significantly, depending on the exposure route. We did not expect the response to ingested NPs, due to the low exposure concentrations (100 µg/g dry weight food) and a firm gut epithelia, along with a lack of phagocytosis in the digestive system, which would theoretically prevent NPs from crossing the biological barrier. It remains a challenge for future research to reveal what the physiological and ecological significance is for the organism to sense and respond, via the immune system, to ingested foreign material.
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- 2022
19. Interaction of Metallic Nanoparticles With Biomimetic Lipid Liquid Crystalline Cubic Interfaces
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Cardellini, Jacopo, Montis, Costanza, Barbero, Francesco, De Santis, Ilaria, Caselli, Lucrezia, Berti, Debora, Cardellini, Jacopo, Montis, Costanza, Barbero, Francesco, De Santis, Ilaria, Caselli, Lucrezia, and Berti, Debora
- Abstract
In the past decades, events occurring at the nano-bio interface (i.e., where engineered nanoparticles (NPs) meet biological interfaces such as biomembranes) have been intensively investigated, to address the cytotoxicity of nanomaterials and boost their clinical translation. In this field, lamellar synthetic model membranes have been instrumental to disentangle non-specific interactions between NPs and planar biological interfaces. Much less is known on nano-biointeractions occurring at highly curved biological interfaces, such as cubic membranes. These non-lamellar architectures play a crucial -but far from understood-role in several biological processes and occur in cells as a defence mechanism against bacterial and viral pathologies, including coronaviruses infections. Despite its relevance, the interaction of cubic membranes with nano-sized objects (such as viral pathogens, biological macromolecules and synthetic NPs) remains largely unexplored to date. Here, we address the interaction of model lipid cubic phase membranes with two prototypical classes of NPs for Nanomedicine, i.e., gold (AuNPs) and silver NPs (AgNPs). To this purpose, we challenged lipid cubic phase membranes, either in the form of dispersed nanoparticles (i.e., cubosomes) or solid-supported layers of nanometric thickness, with citrate-stabilized AuNPs and AgNPs and monitored the interaction combining bulk techniques (UV-visible spectroscopy, Light and Synchrotron Small-Angle X-ray Scattering) with surface methods (Quartz Crystal Microbalance and Confocal Laser Scanning Microscopy). We show that the composition of the metal core of NPs (i.e., Au vs Ag) modulates their adsorption and self-assembly at cubic interfaces, leading to an extensive membrane-induced clustering of AuNPs, while only to a mild adsorption of isolated AgNPs. Such differences mirror opposite effects at the membrane level, where AuNPs induce lipid extraction followed by a fast disruption of the cubic assembly, while AgNPs do
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- 2022
20. A Biomonitoring Pilot Study in Workers from a Paints Production Plant Exposed to Pigment-Grade Titanium Dioxide (TiO2)
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Bergamaschi, Enrico, primary, Bellisario, Valeria, additional, Macrì, Manuela, additional, Buglisi, Martina, additional, Garzaro, Giacomo, additional, Squillacioti, Giulia, additional, Ghelli, Federica, additional, Bono, Roberto, additional, Fenoglio, Ivana, additional, Barbero, Francesco, additional, Riganti, Chiara, additional, Marrocco, Antonella, additional, Bonetta, Sara, additional, and Carraro, Elisabetta, additional
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- 2022
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21. Interaction of Metallic Nanoparticles With Biomimetic Lipid Liquid Crystalline Cubic Interfaces
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Cardellini, Jacopo, primary, Montis, Costanza, additional, Barbero, Francesco, additional, De Santis, Ilaria, additional, Caselli, Lucrezia, additional, and Berti, Debora, additional
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- 2022
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22. Additional file 1 of Changes of physico-chemical properties of nano-biomaterials by digestion fluids affect the physiological properties of epithelial intestinal cells and barrier models
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Antonello, Giulia, Marucco, Arianna, Gazzano, Elena, Kainourgios, Panagiotis, Ravagli, Costanza, Gonzalez-Paredes, Ana, Sprio, Simone, Padín-González, Esperanza, Soliman, Mahmoud G., Beal, David, Barbero, Francesco, Gasco, Paolo, Baldi, Giovanni, Carriere, Marie, Monopoli, Marco P., Charitidis, Costas A., Bergamaschi, Enrico, Fenoglio, Ivana, and Riganti, Chiara
- Abstract
Additional file 1. Figure S1. Optical appearance of the colloidal suspensions before A) and after B) treatment with the SHDS. Figure S2. Size distribution of the samples directly incubated in SIF or after complete SHDS-treatment of A) LSNPs; B) CNPs, C) FNPs; D) HNPs. Figure S3. HNPs dissolution. Figure S4. Degradation of LSNPs by lipase. Table S1. Mean dH and PDI of NMs in DMEM+10% FBS (100 mg/ml), 24 h incubation. Figure S5. Aspect of the suspension of the (*) untreated and (**) SHDS-treated A) LSNPs, B) CNPs, C) FNPs and D) HNPs in cell medium. Figure S6. SDS-PAGE analysis showing the hard corona of (*) SHDS-treated and (**) untreated A) LSNPs, B) CNPs, C) FNPs and D) HNPs in cell medium and obtained by centrifugation, and E) ctrl without NBMs. Figure S7. LFQ intensity for different proteases inhibitor for FNPs untreated and SHDS-treated. Figure S8. Cells viability of A) Caco-2 cells and B) HCoEpiC cells after 24 h of incubation with SHDS fluids. Figure S9. Genotoxicity of HCT116 cells after 24 h of incubation with untreated and SHDS-treated NMs. Table S2. Tran-Epithelial Electrical Resistance (TEER) values of Caco-2 barrier model after 24 h of incubation. Figure S10. Scheme of representative SDHS treatment.
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- 2022
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23. Innate Memory Reprogramming by Gold Nanoparticles Depends on the Microbial Agents That Induce Memory
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Swartzwelter, Benjamin J., Michelini, Sara, Frauenlob, Tobias, Barbero, Francesco, Verde, Alessandro, Chiara de Luca, Anna, Puntes, Víctor F., Duschl, Albert, Horejs-Hoeck, Jutta, Italiani, Paola, Boraschi, Diana, University of Salzburg, and Austrian Science Fund
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Innate immunity ,Staphylococcus aureus ,beta-Glucans ,Helicobacter pylori ,Innate memory ,Immunology ,Metal Nanoparticles ,Immunity, Innate ,Monocytes ,Candida albicans ,Cytokines ,Humans ,Nanoparticles ,Gold ,Microbial agents ,Immunologic Memory ,Cells, Cultured ,Original Research - Abstract
Innate immune memory, the ability of innate cells to react in a more protective way to secondary challenges, is induced by exposure to infectious and other exogeous and endogenous agents. Engineered nanoparticles are particulate exogenous agents that, as such, could trigger an inflammatory reaction in monocytes and macrophages and could therefore be also able to induce innate memory. Here, we have evaluated the capacity of engineered gold nanoparticles (AuNPs) to induce a memory response or to modulate the memory responses induced by microbial agents. Microbial agents used were in soluble vs. particulate form (MDP and the gram-positive bacteria Staphylococcus aureus; β-glucan and the β-glucan-producing fungi C. albicans), and as whole microrganisms that were either killed (S. aureus, C. albicans) or viable (the gram-negative bacteria Helicobacter pylori). The memory response was assessed in vitro, by exposing human primary monocytes from 2-7 individual donors to microbial agents with or without AuNPs (primary response), then resting them for 6 days to allow return to baseline, and eventually challenging them with LPS (secondary memory response). Primary and memory responses were tested as production of the innate/inflammatory cytokine TNFα and other inflammatory and anti-inflammatory factors. While inactive on the response induced by soluble microbial stimuli (muramyl dipeptide -MDP-, β-glucan), AuNPs partially reduced the primary response induced by whole microorganisms. AuNPs were also unable to directly induce a memory response but could modulate stimulus-induced memory in a circumscribed fashion, limited to some agents and some cytokines. Thus, the MDP-induced tolerance in terms of TNFα production was further exacerbated by co-priming with AuNPs, resulting in a less inflammatory memory response. Conversely, the H. pylori-induced tolerance was downregulated by AuNPs only relative to the anti-inflammatory cytokine IL-10, which would lead to an overall more inflammatory memory response. These effects of AuNPs may depend on a differential interaction/association between the reactive particle surfaces and the microbial components and agents, which may lead to a change in the exposure profiles. As a general observation, however, the donor-to-donor variability in memory response profiles and reactivity to AuNPs was substantial, suggesting that innate memory depends on the individual history of exposures., This work was supported by the EU Commission H2020 projects PANDORA (GA 671881) and ENDONANO (GA 812661), the Italian MIUR InterOmics Flagship projects MEMORAT and MAME, the Italian MIUR/PRIN-20173ZECCM, the Priority program ACBN (Allergy Cancer BioNano Research Centre) of the University of Salzburg, the Cancer Cluster Salzburg, the Research Grant from the University of Salzburg, and the Austrian Science Fund (FWF) Grant Nr. P 29941.
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- 2021
24. Growth-Promoting Gold Nanoparticles Decrease Stress Responses in Arabidopsis Seedlings
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Ferrari, Eleonora, primary, Barbero, Francesco, additional, Busquets-Fité, Marti, additional, Franz-Wachtel, Mirita, additional, Köhler, Heinz-R., additional, Puntes, Victor, additional, and Kemmerling, Birgit, additional
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- 2021
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25. Innate Memory Reprogramming by Gold Nanoparticles Depends on the Microbial Agents That Induce Memory
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Swartzwelter, Benjamin J., primary, Michelini, Sara, additional, Frauenlob, Tobias, additional, Barbero, Francesco, additional, Verde, Alessandro, additional, De Luca, Anna Chiara, additional, Puntes, Victor, additional, Duschl, Albert, additional, Horejs-Hoeck, Jutta, additional, Italiani, Paola, additional, and Boraschi, Diana, additional
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- 2021
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26. Desorption and transfer processes in different classes of dyes.
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Cova, Camilla Maria, Conti, Laura, Barbero, Francesco, Berti, Debora, and Bianchetti, Giulia Ottavia
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REACTIVE dyes ,TEXTILE dyeing ,DISPERSE dyes ,DESORPTION ,COTTON textiles ,DYES & dyeing - Abstract
We report an analytical approach to study dye desorption and transfer processes in aqueous environment. The study was conducted simulating standard laundry washes using two different donor textiles, a polyester textile dyed with Disperse Blue 79 and a cotton textile dyed with Reactive Red 239, and two acceptor textiles, undyed cotton and polyester, respectively. Dye bleeding/solubilization from a donor textile to the solution was investigated through UV–visible absorbance analysis, while the dye absorption onto acceptor textile was quantified by UV–visible reflectance analysis. Dye desorption and transfer processes were investigated in water and surfactant solutions at different concentrations. A desorption and transfer model for the two classes of dyes was hypothesized by performing kinetic experiments. The results show that both processes depend on the presence of surfactants for the disperse dye. Conversely, the presence of surfactants has little effect on the reactive dye. Finally, the presence of an acceptor polyester significantly increases the bleeding of the disperse dye, while the presence of an acceptor textiles less influences the total release of the reactive dye. [ABSTRACT FROM AUTHOR]
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- 2023
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27. Immunomodulatory Function of Polyvinylpyrrolidone (PVP)-Functionalized Gold Nanoparticles in Vibrio-Stimulated Sea Urchin Immune Cells
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Alijagic, Andi, primary, Bonura, Angela, additional, Barbero, Francesco, additional, Puntes, Victor F., additional, Gervasi, Francesco, additional, and Pinsino, Annalisa, additional
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- 2021
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28. Cross-Species Comparisons of Nanoparticle Interactions with Innate Immune Systems: A Methodological Review
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Swartzwelter, Benjamin J., primary, Mayall, Craig, additional, Alijagic, Andi, additional, Barbero, Francesco, additional, Ferrari, Eleonora, additional, Hernadi, Szabolcs, additional, Michelini, Sara, additional, Navarro Pacheco, Natividad Isabel, additional, Prinelli, Alessandra, additional, Swart, Elmer, additional, and Auguste, Manon, additional
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- 2021
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29. Formation and evolution of the nanoparticle environmental corona: The case of Au and humic acid
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Barbero, Francesco, primary, Mayall, Craig, additional, Drobne, Damjana, additional, Saiz-Poseu, Javier, additional, Bastús, Neus G., additional, and Puntes, Victor, additional
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- 2021
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30. Cross-species comparisons of nanoparticle interactions with innate immune systems: A methodological review
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Swartzwelter, Benjamin J., Mayall, Craig, Alijagic, Andi, Barbero, Francesco, Ferrari, Eleonora, Hernadi, Szabolcs, Michelini, Sara, Navarro Pacheco, Natividad I., Prinelli, Alessandra, Swart, Elmer, Auguste, Manon, and European Commission
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Innate immunity ,Markers ,NPs testing ,Environmental models ,Humen cells - Abstract
Many components of the innate immune system are evolutionarily conserved and shared across many living organisms, from plants and invertebrates to humans. Therefore, these shared features can allow the comparative study of potentially dangerous substances, such as engineered nanoparticles (NPs). However, differences of methodology and procedure between diverse species and models make comparison of innate immune responses to NPs between organisms difficult in many cases. To this aim, this review provides an overview of suitable methods and assays that can be used to measure NP immune interactions across species in a multidisciplinary approach. The first part of this review describes the main innate immune defense characteristics of the selected models that can be associated to NPs exposure. In the second part, the different modes of exposure to NPs across models (considering isolated cells or whole organisms) and the main endpoints measured are discussed. In this synergistic perspective, we provide an overview of the current state of important cross-disciplinary immunological models to study NP-immune interactions and identify future research needs. As such, this paper could be used as a methodological reference point for future nano-immunosafety studies. All authors were supported by the EU H2020 project PANDORA, grant number 671881.
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- 2021
31. Gold nanoparticles coated with polyvinylpyrrolidone and sea urchin extracellular molecules induce transient immune activation
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Alijagic, Andi, Barbero, Francesco, Gaglio, Daniela, Napodano, Elisabetta, Benada, Oldřich, Kofroňová, Olga, Puntes, Victor F, Bastús, Neus G, Pinsino, Annalisa, Alijagic, Andi, Barbero, Francesco, Gaglio, Daniela, Napodano, Elisabetta, Benada, Oldřich, Kofroňová, Olga, Puntes, Victor F, Bastús, Neus G, and Pinsino, Annalisa
- Abstract
We report that the immunogenicity of colloidal gold nanoparticles coated with polyvinylpyrrolidone (PVP-AuNPs) in a model organism, the sea urchin Paracentrotus lividus, can function as a proxy for humans for in vitro immunological studies. To profile the immune recognition and interaction from exposure to PVP-AuNPs (1 and 10 μg mL-1), we applied an extensive nano-scale approach, including particle physicochemical characterisation involving immunology, cellular biology, and metabolomics. The interaction between PVP-AuNPs and soluble proteins of the sea urchin physiological coelomic fluid (blood equivalent) results in the formation of a protein "corona" surrounding the NPs from three major proteins that influence the hydrodynamic size and colloidal stability of the particle. At the lower concentration of PVP-AuNPs, the P. lividus phagocytes show a broad metabolic plasticity based on the biosynthesis of metabolites mediating inflammation and phagocytosis. At the higher concentration of PVP-AuNPs, phagocytes activate an immunological response involving Toll-like receptor 4 (TLR4) signalling pathway at 24 hours of exposure. These results emphasise that exposure to PVP-AuNPs drives inflammatory signalling by the phagocytes and the resolution at both the low and high concentrations of the PVP-AuNPs and provides more details regarding the immunogenicity of these NPs.
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- 2021
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32. Cross-species comparisons of nanoparticle interactions with innate immune systems : a methodological review
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Swartzwelter, Benjamin, Mayall, Craig, Alijagic, Andi, Barbero, Francesco, Ferrari, Eleonora, Hernadi, Szabolcs, Michelini, Sara, Navarro Pacheco, Natividad Isabel, Prinelli, Alessandra, Swart, Elmer, Auguste, Manon, Swartzwelter, Benjamin, Mayall, Craig, Alijagic, Andi, Barbero, Francesco, Ferrari, Eleonora, Hernadi, Szabolcs, Michelini, Sara, Navarro Pacheco, Natividad Isabel, Prinelli, Alessandra, Swart, Elmer, and Auguste, Manon
- Abstract
Many components of the innate immune system are evolutionarily conserved and shared across many living organisms, from plants and invertebrates to humans. Therefore, these shared features can allow the comparative study of potentially dangerous substances, such as engineered nanoparticles (NPs). However, differences of methodology and procedure between diverse species and models make comparison of innate immune responses to NPs between organisms difficult in many cases. To this aim, this review provides an overview of suitable methods and assays that can be used to measure NP immune interactions across species in a multidisciplinary approach. The first part of this review describes the main innate immune defense characteristics of the selected models that can be associated to NPs exposure. In the second part, the different modes of exposure to NPs across models (considering isolated cells or whole organisms) and the main endpoints measured are discussed. In this synergistic perspective, we provide an overview of the current state of important cross-disciplinary immunological models to study NP-immune interactions and identify future research needs. As such, this paper could be used as a methodological reference point for future nano-immunosafety studies.
- Published
- 2021
33. Functional and Morphological Changes Induced in Mytilus Hemocytes by Selected Nanoparticles
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Auguste, Manon, Mayall, Craig, Barbero, Francesco, Hočevar, Matej, Alberti, Stefano, Grassi, Giacomo, Puntes, Víctor, Drobne, Damjana, Canesi, Laura, Auguste, Manon, Mayall, Craig, Barbero, Francesco, Hočevar, Matej, Alberti, Stefano, Grassi, Giacomo, Puntes, Víctor, Drobne, Damjana, and Canesi, Laura
- Abstract
Nanoparticles (NPs) show various properties depending on their composition, size, and surface coating, which shape their interactions with biological systems. In particular, NPs have been shown to interact with immune cells, that represent a sensitive surveillance system of external and internal stimuli. In this light, in vitro models represent useful tools for investigating nano-bio-interactions in immune cells of different organisms, including invertebrates. In this work, the effects of selected types of NPs with different core composition, size and functionalization (custom-made PVP-AuNP and commercial nanopolystyrenes PS-NH and PS-COOH) were investigated in the hemocytes of the marine bivalve Mytilus galloprovincialis. The role of exposure medium was evaluated using either artificial seawater (ASW) or hemolymph serum (HS). Hemocyte morphology was investigated by scanning electron microscopy (SEM) and different functional parameters (lysosomal membrane stability, phagocytosis, and lysozyme release) were evaluated. The results show distinct morphological and functional changes induced in mussel hemocytes depending on the NP type and exposure medium. Mussel hemocytes may represent a powerful alternative in vitro model for a rapid pre-screening strategy for NPs, whose utilization will contribute to the understanding of the possible impact of environmental exposure to NPs in marine invertebrates.
- Published
- 2021
34. Gold nanoparticles (AuNPs) impair LPS-driven immune responses by promoting a tolerogenic-like dendritic cell phenotype with altered endosomal structures
- Author
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European Commission, Austrian Science Fund, Michelini, Sara, Barbero, Francesco, Prinelli, Alessandra, Steiner, Paul, Weiss, Richard, Verwanger, Thomas, Andosch, Ancuela, Lütz-Meindl, Ursula, Puntes, Víctor F., Drobne, Damjana, Duschl, Albert, Horejs-Hoeck, Jutta, European Commission, Austrian Science Fund, Michelini, Sara, Barbero, Francesco, Prinelli, Alessandra, Steiner, Paul, Weiss, Richard, Verwanger, Thomas, Andosch, Ancuela, Lütz-Meindl, Ursula, Puntes, Víctor F., Drobne, Damjana, Duschl, Albert, and Horejs-Hoeck, Jutta
- Abstract
Dendritic cells (DCs) shape immune responses by influencing T-cell activation. Thus, they are considered both an interesting model for studying nano-immune interactions and a promising target for nano-based biomedical applications. However, the accentuated ability of nanoparticles (NPs) to interact with biomolecules may have an impact on DC function that poses an unexpected risk of unbalanced immune reactions. Here, we investigated the potential effects of gold nanoparticles (AuNPs) on DC function and the consequences for effector and memory T-cell responses in the presence of the microbial inflammatory stimulus lipopolysaccharide (LPS). Overall, we found that, in the absence of LPS, none of the tested NPs induced a DC response. However, whereas 4-, 8-, and 11 nm AuNPs did not modulate LPS-dependent immune responses, 26 nm AuNPs shifted the phenotype of LPS-activated DCs toward a tolerogenic state, characterized by downregulation of CD86, IL-12 and IL-27, upregulation of ILT3, and induction of class E compartments. Moreover, this DC phenotype was less proficient in promoting Th1 activation and central memory T-cell proliferation. Taken together, these findings support the perception that AuNPs are safe under homeostatic conditions; however, particular care should be taken in patients experiencing a current infection or disorders of the immune system.
- Published
- 2021
35. Growth-promoting gold nanoparticles decrease stress responses in arabidopsis seedlings
- Author
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European Commission, Ferrari, Eleonora, Barbero, Francesco, Busquets-Fité, Martí, Franz-Wachtel, Mirita, Köhler, Heinz-R., Puntes, Víctor F., Kemmerling, Birgit, European Commission, Ferrari, Eleonora, Barbero, Francesco, Busquets-Fité, Martí, Franz-Wachtel, Mirita, Köhler, Heinz-R., Puntes, Víctor F., and Kemmerling, Birgit
- Abstract
The global economic success of man-made nanoscale materials has led to a higher production rate and diversification of emission sources in the environment. For these reasons, novel nanosafety approaches to assess the environmental impact of engineered nanomaterials are required. While studying the potential toxicity of metal nanoparticles (NPs), we realized that gold nanoparticles (AuNPs) have a growth-promoting rather than a stress-inducing effect. In this study we established stable short-and long-term exposition systems for testing plant responses to NPs. Exposure of plants to moderate concentrations of AuNPs resulted in enhanced growth of the plants with longer primary roots, more and longer lateral roots and increased rosette diameter, and reduced oxidative stress responses elicited by the immune-stimulatory PAMP flg22. Our data did not reveal any detri-mental effects of AuNPs on plants but clearly showed positive effects on growth, presumably by their protective influence on oxidative stress responses. Differential transcriptomics and proteomics analyses revealed that oxidative stress responses are downregulated whereas growth-promoting genes/proteins are upregulated. These omics datasets after AuNP exposure can now be exploited to study the underlying molecular mechanisms of AuNP-induced growth-promotion.
- Published
- 2021
36. Cross-species comparisons of nanoparticle interactions with innate immune systems: A methodological review
- Author
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European Commission, Swartzwelter, Benjamin J., Mayall, Craig, Alijagic, Andi, Barbero, Francesco, Ferrari, Eleonora, Hernadi, Szabolcs, Michelini, Sara, Navarro Pacheco, Natividad I., Prinelli, Alessandra, Swart, Elmer, Auguste, Manon, European Commission, Swartzwelter, Benjamin J., Mayall, Craig, Alijagic, Andi, Barbero, Francesco, Ferrari, Eleonora, Hernadi, Szabolcs, Michelini, Sara, Navarro Pacheco, Natividad I., Prinelli, Alessandra, Swart, Elmer, and Auguste, Manon
- Abstract
Many components of the innate immune system are evolutionarily conserved and shared across many living organisms, from plants and invertebrates to humans. Therefore, these shared features can allow the comparative study of potentially dangerous substances, such as engineered nanoparticles (NPs). However, differences of methodology and procedure between diverse species and models make comparison of innate immune responses to NPs between organisms difficult in many cases. To this aim, this review provides an overview of suitable methods and assays that can be used to measure NP immune interactions across species in a multidisciplinary approach. The first part of this review describes the main innate immune defense characteristics of the selected models that can be associated to NPs exposure. In the second part, the different modes of exposure to NPs across models (considering isolated cells or whole organisms) and the main endpoints measured are discussed. In this synergistic perspective, we provide an overview of the current state of important cross-disciplinary immunological models to study NP-immune interactions and identify future research needs. As such, this paper could be used as a methodological reference point for future nano-immunosafety studies.
- Published
- 2021
37. Formation and evolution of the nanoparticle environmental corona: The case of Au and humic acid
- Author
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European Commission, Ministerio de Economía y Competitividad (España), Generalitat de Catalunya, Agencia Estatal de Investigación (España), Ministerio de Ciencia, Innovación y Universidades (España), Barbero, Francesco, Mayall, Craig, Drobne, Damjana, Saiz-Poseu, Javier, Bastús, Neus G., Puntes, Víctor F., European Commission, Ministerio de Economía y Competitividad (España), Generalitat de Catalunya, Agencia Estatal de Investigación (España), Ministerio de Ciencia, Innovación y Universidades (España), Barbero, Francesco, Mayall, Craig, Drobne, Damjana, Saiz-Poseu, Javier, Bastús, Neus G., and Puntes, Víctor F.
- Abstract
Studying the behaviour of nanomaterials after their release into natural water is essential to understand the risk associated to their environmental exposure. In particular, the interaction and adsorption of dissolved organic matter onto nanoparticles strongly influence the behaviour and fate of nanomaterials in natural water systems. We herein study the interaction of Au and Ag nanoparticles and humic acids, the principal component of natural dissolved organic matter. Physicochemical characterization results showed the formation of an organic matter corona, consisting of two layers: a “hard” one, firmly bound to the nanoparticle surface, and a “soft” one, in dynamic equilibrium and, consequently, highly dependent on the media organic matter concentration. The extent of the electro-steric stabilization of the so called environmental corona depends on the size of the supramolecular association of humic acid (which depends on its hydrophilic and lipophilic moieties), the nanoparticle size, the total concentration of organic matter in the media, and the ratio between them. Interestingly, environmental coronas can eventually prevent Ca2+ and Mg2+ induced aggregation at concentrations range present in most of the freshwater bodies. The humic coating formed on top of the Au or control Ag nanoparticles presented a similar profile, but the corrodibility of Ag led to a more natural detachment of the corona. These results were further confirmed by exposing the nanoparticles to a model of natural water and standard mud (LUFA 2.2 dispersion). In the latter case, after several days, nanoparticle sedimentation was observed, which was attributed to interactions with macro organic and inorganic matter (fraction larger than particulate matter).
- Published
- 2021
38. Aquantitative assessment of stochastic electrodynamics with spin (SEDS): Physical principles and novel applications
- Author
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Cavalleri, Giancarlo, Barbero, Francesco, Bertazzi, Gianfranco, Cesaroni, Eros, Tonni, Ernesto, Bosi, Leonardo, Spavieri, Gianfranco, and Gillies, George T.
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- 2010
- Full Text
- View/download PDF
39. Stressor-Dependant Changes in Immune Parameters in the Terrestrial Isopod Crustacean, Porcellio scaber: A Focus on Nanomaterials
- Author
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Mayall, Craig, primary, Dolar, Andraz, additional, Jemec Kokalj, Anita, additional, Novak, Sara, additional, Razinger, Jaka, additional, Barbero, Francesco, additional, Puntes, Victor, additional, and Drobne, Damjana, additional
- Published
- 2021
- Full Text
- View/download PDF
40. Functional and Morphological Changes Induced in Mytilus Hemocytes by Selected Nanoparticles
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Auguste, Manon, primary, Mayall, Craig, additional, Barbero, Francesco, additional, Hočevar, Matej, additional, Alberti, Stefano, additional, Grassi, Giacomo, additional, Puntes, Victor, additional, Drobne, Damjana, additional, and Canesi, Laura, additional
- Published
- 2021
- Full Text
- View/download PDF
41. Addressing nanomaterial immunosafety by evaluating innate immunity across living species
- Author
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Boraschi, Diana, Alijagic, Andi, Auguste, Manon, Barbero, Francesco, Ferrari, Eleonora, Hernadi, Szabolcs, Mayall, Craig, Michelini, Sara, Navarro Pacheco, Natividad I., Prinelli, Alessandra, Swart, Elmer, Swartzwelter, Benjamin J., Bastús, Neus G., Canesi, Laura, Drobne, Damjana, Duschl, Albert, Ewart, Marie‐Ann, Horejs‐Hoeck, Jutta, Italiani, Paola, Kemmerling, Birgit, Kille, Peter, Prochazkova, Petra, Puntes, Victor F., Spurgeon, David J., Svendsen, Claus, Wilde, Colin J., Pinsino, Annalisa, Boraschi, Diana, Alijagic, Andi, Auguste, Manon, Barbero, Francesco, Ferrari, Eleonora, Hernadi, Szabolcs, Mayall, Craig, Michelini, Sara, Navarro Pacheco, Natividad I., Prinelli, Alessandra, Swart, Elmer, Swartzwelter, Benjamin J., Bastús, Neus G., Canesi, Laura, Drobne, Damjana, Duschl, Albert, Ewart, Marie‐Ann, Horejs‐Hoeck, Jutta, Italiani, Paola, Kemmerling, Birgit, Kille, Peter, Prochazkova, Petra, Puntes, Victor F., Spurgeon, David J., Svendsen, Claus, Wilde, Colin J., and Pinsino, Annalisa
- Abstract
The interaction of a living organism with external foreign agents is a central issue for its survival and adaptation to the environment. Nanosafety should be considered within this perspective, and it should be examined that how different organisms interact with engineered nanomaterials (NM) by either mounting a defensive response or by physiologically adapting to them. Herein, the interaction of NM with one of the major biological systems deputed to recognition of and response to foreign challenges, i.e., the immune system, is specifically addressed. The main focus is innate immunity, the only type of immunity in plants, invertebrates, and lower vertebrates, and that coexists with adaptive immunity in higher vertebrates. Because of their presence in the majority of eukaryotic living organisms, innate immune responses can be viewed in a comparative context. In the majority of cases, the interaction of NM with living organisms results in innate immune reactions that eliminate the possible danger with mechanisms that do not lead to damage. While in some cases such interaction may lead to pathological consequences, in some other cases beneficial effects can be identified.
- Published
- 2020
42. Addressing nanomaterial immunosafety by evaluating innate immunity across living species
- Author
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Ministero dell'Istruzione, dell'Università e della Ricerca, Austrian Science Fund, Generalitat de Catalunya, Ministerio de Economía y Competitividad (España), Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), European Commission, Ministry of Education, Youth and Sports (Czech Republic), Boraschi, Diana, Alijagic, Andi, Auguste, Manon, Barbero, Francesco, Ferrari, Eleonora, Hernadi, Szabolcs, Mayall, Craig, Michelini, Sara, Navarro Pacheco, Natividad I., Prinelli, Alessandra, Swart, Elmer, Swartzwelter, Benjamin J., Bastús, Neus G., Canesi, Laura, Drobne, Damjana, Duschl, Albert, Ewart, Marie-Ann, Horejs-Hoeck, Jutta, Italiani, Paola, Kemmerling, Birgit, Kille, Peter, Prochazkova, Petra, Puntes, Víctor F., Spurgeon, David J., Svendsen, Claus, Wilde, Colin J., Pinsino, Annalisa, Ministero dell'Istruzione, dell'Università e della Ricerca, Austrian Science Fund, Generalitat de Catalunya, Ministerio de Economía y Competitividad (España), Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), European Commission, Ministry of Education, Youth and Sports (Czech Republic), Boraschi, Diana, Alijagic, Andi, Auguste, Manon, Barbero, Francesco, Ferrari, Eleonora, Hernadi, Szabolcs, Mayall, Craig, Michelini, Sara, Navarro Pacheco, Natividad I., Prinelli, Alessandra, Swart, Elmer, Swartzwelter, Benjamin J., Bastús, Neus G., Canesi, Laura, Drobne, Damjana, Duschl, Albert, Ewart, Marie-Ann, Horejs-Hoeck, Jutta, Italiani, Paola, Kemmerling, Birgit, Kille, Peter, Prochazkova, Petra, Puntes, Víctor F., Spurgeon, David J., Svendsen, Claus, Wilde, Colin J., and Pinsino, Annalisa
- Abstract
The interaction of a living organism with external foreign agents is a central issue for its survival and adaptation to the environment. Nanosafety should be considered within this perspective, and it should be examined that how different organisms interact with engineered nanomaterials (NM) by either mounting a defensive response or by physiologically adapting to them. Herein, the interaction of NM with one of the major biological systems deputed to recognition of and response to foreign challenges, i.e., the immune system, is specifically addressed. The main focus is innate immunity, the only type of immunity in plants, invertebrates, and lower vertebrates, and that coexists with adaptive immunity in higher vertebrates. Because of their presence in the majority of eukaryotic living organisms, innate immune responses can be viewed in a comparative context. In the majority of cases, the interaction of NM with living organisms results in innate immune reactions that eliminate the possible danger with mechanisms that do not lead to damage. While in some cases such interaction may lead to pathological consequences, in some other cases beneficial effects can be identified.
- Published
- 2020
43. Gold nanoparticles coated with polyvinylpyrrolidone and sea urchin extracellular molecules induce transient immune activation
- Author
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Alijagic, Andi, primary, Barbero, Francesco, additional, Gaglio, Daniela, additional, Napodano, Elisabetta, additional, Benada, Oldřich, additional, Kofroňová, Olga, additional, Puntes, Victor F., additional, Bastús, Neus G., additional, and Pinsino, Annalisa, additional
- Published
- 2021
- Full Text
- View/download PDF
44. Gold nanoparticles (AuNPs) impair LPS-driven immune responses by promoting a tolerogenic-like dendritic cell phenotype with altered endosomal structures
- Author
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Michelini, Sara, primary, Barbero, Francesco, additional, Prinelli, Alessandra, additional, Steiner, Philip, additional, Weiss, Richard, additional, Verwanger, Thomas, additional, Andosch, Ancuela, additional, Lütz-Meindl, Ursula, additional, Puntes, Victor F., additional, Drobne, Damjana, additional, Duschl, Albert, additional, and Horejs-Hoeck, Jutta, additional
- Published
- 2021
- Full Text
- View/download PDF
45. Addressing Nanomaterial Immunosafety by Evaluating Innate Immunity across Living Species
- Author
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Boraschi, Diana, primary, Alijagic, Andi, additional, Auguste, Manon, additional, Barbero, Francesco, additional, Ferrari, Eleonora, additional, Hernadi, Szabolcs, additional, Mayall, Craig, additional, Michelini, Sara, additional, Navarro Pacheco, Natividad I., additional, Prinelli, Alessandra, additional, Swart, Elmer, additional, Swartzwelter, Benjamin J., additional, Bastús, Neus G., additional, Canesi, Laura, additional, Drobne, Damjana, additional, Duschl, Albert, additional, Ewart, Marie‐Ann, additional, Horejs‐Hoeck, Jutta, additional, Italiani, Paola, additional, Kemmerling, Birgit, additional, Kille, Peter, additional, Prochazkova, Petra, additional, Puntes, Victor F., additional, Spurgeon, David J., additional, Svendsen, Claus, additional, Wilde, Colin J., additional, and Pinsino, Annalisa, additional
- Published
- 2020
- Full Text
- View/download PDF
46. Gold Nanoparticles Modulate BCG-Induced Innate Immune Memory in Human Monocytes by Shifting the Memory Response towards Tolerance
- Author
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Swartzwelter, Benjamin J., primary, Barbero, Francesco, additional, Verde, Alessandro, additional, Mangini, Maria, additional, Pirozzi, Marinella, additional, De Luca, Anna Chiara, additional, Puntes, Victor F., additional, Leite, Luciana C. C., additional, Italiani, Paola, additional, and Boraschi, Diana, additional
- Published
- 2020
- Full Text
- View/download PDF
47. Physicochemical characterization of the evolution of metal nanoparticles in biological and environmental media: from synthesis to interaction with living organisms
- Author
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Barbero, Francesco, Puntes, Víctor, Gómez Bastús, Neus, and Universitat Autònoma de Barcelona. Departament de Química
- Subjects
Ciències Experimentals ,Protein corona ,Nanoparticules ,Nanoparticles ,Nanoparticulas - Abstract
La producció creixent de Nanopartícules (NPs) conduirà inevitablement a un augment d’exposició humana i ambiental a aquests materials. En conseqüència, han sorgit preocupacions raonables sobre els seus possibles riscos per a la seguretat, donant lloc a la disciplina de nanotoxicologia/nanoseguretat. A causa de l’alta reactivitat, les NP exposades a diferents escenaris biològics i ambientals, solen arribar a un estat termodinàmic més estable mitjançant l’agregació, la interacció amb les molècules presents al medi, l’adsorció a matèria macro-orgànica, les transformacions químiques i la dissolució. Totes aquestes transformacions poden generar una nova identitat dels nanoobjectes o produir noves entitats químiques, canviant així el seu comportament i, per tant, el risc potencial associat. Així, els mateixos NP poden tenir un destí totalment diferent i, en conseqüència, un impacte totalment diferent sobre els organismes vius i el medi ambient depenent del microambient (per exemple, el medi d’exposició) on es trobin. A més, les característiques primigènies del nanomaterial influeixen molt en la seva destinació biològica i ambiental. Des d’aquesta perspectiva, és fonamental comprendre les característiques de l’objecte final que toparà amb els organismes vius i analitzar les seves propietats, per tal de correlacionar les característiques prèvies i finals de NP amb els efectes potencials sobre els organismes vius. En aquest context, aquesta tesi s’ha centrat en la transformació fisicoquímica dels NP model exposats a medis biològics i ambientals. Per a aquests estudis, es van triar NPs Au i Ag, ja que són models NP molt utilitzats i per les seves nombroses aplicacions. En primer lloc, l’estudi es va centrar en la influència de la composició de medis de cultiu cel·lular en el procés de formació de protein corona (PC), la composició final i l’estat d’agregació de NPs i els efectes consegüents sobre la captació de cèl·lules NP. També es va realitzar una caracterització fisicoquímica de la naturalesa de la bicapa CTAB - Au NP Au per estudiar l’impacte d’aquest recobriment de superfície NP àmpliament utilitzat en l’exposició de la partícula a fluids biològics, en la formació de la proteïna corona i en el disseny i interpretació de Proves de toxicitat NP. Finalment, es va explorar l’evolució del NP en aigua dolça natural mitjançant un estudi de la naturalesa d’interacció de les NP i de la matèria orgànica natural i de les característiques derivades del NP. La creciente producción de nanopartículas (NP) conducirá inevitablemente a un aumento de la exposición humana y ambiental a estos materiales. En consecuencia, han surgido preocupaciones razonables con respecto a sus posibles riesgos de seguridad, dando lugar a la disciplina de nanotoxicología/nanoseguridad. Debido a la alta reactividad, los NP expuestos a diferentes escenarios biológicos y ambientales tienden a alcanzar un estado termodinámico más estable a través de la agregación, la interacción con las moléculas presentes en el medio ambiente, la adsorción a la materia macro-orgánica, las transformaciones químicas y la disolución. Todas estas transformaciones pueden generar una nueva identidad de los nanoobjetos o producir nuevas entidades químicas, cambiando así su comportamiento y, en consecuencia, su riesgo asociado potencial. Por lo tanto, los mismos NP pueden tener un destino totalmente diferente y, en consecuencia, un impacto totalmente diferente en los organismos vivos y el medio ambiente dependiendo del microambiente (por ejemplo, el medio de exposición) en el que se encuentran. Además, las características prístinas del nanomaterial influyen mucho en su destino biológico y medioambiental. Desde esta perspectiva, resulta fundamental comprender las características del objeto final que encontrará organismos vivos y analizar sus propiedades, a fin de correlacionar las características de NP prístinas y finales con los posibles efectos sobre los organismos vivos. En este contexto, el objetivo de esta tesis ha sido el estudio de la transformacion fisicoquímico de NP modelo expuestos a medios biológicos y ambientales. Para estos estudios, se eligieron NPs de Au y Ag, ya que son modelos de NP ampliamente utilizados y debido a sus numerosas aplicaciones. En primer lugar, el estudio se centró en la influencia de la composición de los medios de cultivo celular en el proceso de formación de protein corona, la composición final y el estado de agregación de NP y los efectos consiguientes en la absorción de células NP. También se realizó una caracterización fisicoquímica de la naturaleza de la bicapa CTAB - Au NP para estudiar el impacto de este recubrimiento de superficie NP ampliamente utilizado en la exposición de la partícula a los fluidos biológicos, en la formación de la corona de proteínas y en el diseño e interpretación de Pruebas de toxicidad NP. Finalmente, la evolución de NP en agua dulce natural se exploró mediante la realización de un estudio de la naturaleza de interacción de NP y materia orgánica natural y las características derivadas de NP. The increasing production of engineered Nanoparticles (NPs) will inevitably lead to an increase of human and environmental exposition to these materials. Consequently reasonable concerns have arisen regarding their potential safety risks, giving rise to the nanotoxicology/nanosafety discipline. Because of the high reactivity, NPs exposed to different biological and environmental scenarios, tend to reach a more stable thermodynamic state via aggregation, interaction with the molecules present in the environment, adsorption to macro-organic matter, chemical transformations and dissolution. All these transformations can generate a new identity of the nano-objects or produce new chemical entities, thereby changing their behaviour and consequently their potential associated risk. Thus, the same NPs can have a totally different fate and consequently a totally different impact on living organisms and the environment depending on the microenvironment (e.g., the exposure medium) in which they are. Furthermore, the pristine features of nano-material highly influence their biological and environmental fate. From this perspective, it becomes fundamental to understand the characteristics of the final object that will encounter living organisms and analyze its properties, in order to correlate the pristine and final NP features with the potential effects on living organisms. In this context, the focus of this thesis has been on the physicochemical transformation of model NPs exposed to biological and environmental media. For these studies, Au and Ag NPs were chosen as they are widely used NP models and because of their numerous applications. Firstly, the study focused on the influence of the cell culture media composition on the protein corona (PC) formation process, final composition and NPs aggregation state and the consequent effects on NP cell uptake. A physicochemical characterization of the nature of the CTAB - Au NP bilayer was also carried out to study the impact of this widely used NP surface coating on the particle’s exposition to biological fluids, on the formation of the protein corona and on the design and interpretation of NP toxicity tests. Finally, the NP evolution in natural fresh water was explored by carrying out a study of the interaction nature of NPs and natural organic matter and the deriving NP features.
- Published
- 2019
48. Dynamic Equilibrium in the Cetyltrimethylammonium Bromide–Au Nanoparticle Bilayer, and the Consequent Impact on the Formation of the Nanoparticle Protein Corona
- Author
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Barbero, Francesco, primary, Moriones, Oscar H., additional, Bastús, Neus G., additional, and Puntes, Victor, additional
- Published
- 2019
- Full Text
- View/download PDF
49. Inclusion of oligonucleotide antimicrobials in biocompatible cationic liposomes: A structural study
- Author
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Mamusa, Marianna, primary, Barbero, Francesco, additional, Montis, Costanza, additional, Cutillo, Laura, additional, Gonzalez-Paredes, Ana, additional, and Berti, Debora, additional
- Published
- 2017
- Full Text
- View/download PDF
50. Formation of the Protein Corona: The Interface between Nanoparticles and the Immune System
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Barbero, Francesco, primary, Russo, Lorenzo, additional, Vitali, Michele, additional, Piella, Jordi, additional, Salvo, Ignacio, additional, Borrajo, Mireya L., additional, Busquets-Fité, Marti, additional, Grandori, Rita, additional, Bastús, Neus G., additional, Casals, Eudald, additional, and Puntes, Victor, additional
- Published
- 2017
- Full Text
- View/download PDF
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