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2. Comparison of microbiological profile of enterotoxigenic Bacteroides fragilis (ETBF) isolates from subjects with colorectal cancer (CRC) or intestinal pre-cancerous lesions versus healthy individuals and evaluation of environmental factors involved in intestinal dysbiosis

Author

Spigaglia, Patrizia, Barbanti, Fabrizio, Germinario, Elena Angela Pia, Criscuolo, Enrico Maria, Bruno, Giovanni, Sanchez-Mete, Lupe, Porowska, Barbara, Stigliano, Vittoria, Accarpio, Fabio, Oddi, Andrea, Zingale, Ilaria, Rossi, Silvia, De Angelis, Roberta, and Fabbri, Alessia

Published
2023
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3. Comparison of Clostridioides difficile strains from animals and humans: First results after introduction of C. difficile molecular typing and characterization at the Istituto Zooprofilattico Sperimentale of Piemonte, Liguria e Valle d’Aosta, Italy

Author

Tramuta, Clara, Spigaglia, Patrizia, Barbanti, Fabrizio, Bianchi, Daniela Manila, Boteva, Cvetelina, Di Blasio, Alessia, Zoppi, Simona, Zaccaria, Teresa, Proroga, Yolande Thérèse Rose, Chiavacci, Laura, Dondo, Alessandro, and Decastelli, Lucia

Published
2021
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7. Antibiotic Resistances of Clostridium difficile

Author

Spigaglia, Patrizia, Mastrantonio, Paola, Barbanti, Fabrizio, COHEN, IRUN R., Series Editor, LAJTHA, ABEL, Series Editor, LAMBRIS, JOHN D., Series Editor, PAOLETTI, RODOLFO, Series Editor, REZAEI, NIMA, Series Editor, Mastrantonio, Paola, editor, and Rupnik, Maja, editor

Published
2018
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16. Genome analysis of Legionella pneumophila ST23 from various countries reveals highly similar strains

Author

Ricci, Maria Luisa, primary, Fillo, Silvia, additional, Ciammaruconi, Andrea, additional, Lista, Florigio, additional, Ginevra, Christophe, additional, Jarraud, Sophie, additional, Girolamo, Antonietta, additional, Barbanti, Fabrizio, additional, Rota, Maria Cristina, additional, Lindsay, Diane, additional, Gorzynski, Jamie, additional, Uldum, Søren A, additional, Baig, Sharmin, additional, Foti, Marina, additional, Petralito, Giancarlo, additional, Torri, Stefania, additional, Faccini, Marino, additional, Bonini, Maira, additional, Gentili, Gabriella, additional, Senatore, Sabrina, additional, Lamberti, Anna, additional, Carrico, Joao André, additional, and Scaturro, Maria, additional

Published
2022
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19. Association of Polygenic Risk Score and Bacterial Toxins at Screening Colonoscopy with Colorectal Cancer Progression: A Multicenter Case-Control Study

Author

Piciocchi, Alfonso, primary, Germinario, Elena Angela Pia, additional, Garcia Etxebarria, Koldo, additional, Rossi, Silvia, additional, Sanchez-Mete, Lupe, additional, Porowska, Barbara, additional, Stigliano, Vittoria, additional, Trentino, Paolo, additional, Oddi, Andrea, additional, Accarpio, Fabio, additional, Grazi, Gian Luca, additional, Bruno, Giovanni, additional, Bonucci, Massimo, additional, Giambenedetti, Massimo, additional, Spigaglia, Patrizia, additional, Barbanti, Fabrizio, additional, Owczarek, Slawomir, additional, Luzzi, Ida, additional, Delibato, Elisabetta, additional, Maroccia, Zaira, additional, Nisticò, Lorenza, additional, Fiorentini, Carla, additional, D’Amato, Mauro, additional, De Angelis, Roberta, additional, and Fabbri, Alessia, additional

Published
2021
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20. Multiplex Real-Time Reverse-Transcription Polymerase Chain Reaction Assays for Diagnostic Testing of Severe Acute Respiratory Syndrome Coronavirus 2 and Seasonal Influenza Viruses: A Challenge of the Phase 3 Pandemic Setting

Author

Mancini, Fabiola, Barbanti, Fabrizio, Scaturro, Maria, Fontana, Stefano, Di Martino, Angela, Marsili, Giulia, Puzelli, Simona, Calzoletti, Laura, Facchini, Marzia, Di Mario, Giuseppina, Fabiani, Concetta, Bella, Antonino, Riccardo, Flavia, Pezzotti, Patrizio, Stefanelli, Paola, Rezza, Giovanni, Ciervo, Alessandra, Villa, Laura, Fortini, Daniela, Iacobino, Angelo, Fiore, Stefano, Benedetti, Eleonora, Marchi, Antonella, Venturi, Giulietta, Fortuna, Claudia, Amendola, Antonello, Toma, Luciano, Di Luca, Marco, and Severini, Francesco

Subjects
biology, business.industry, Orthomyxoviridae, Context (language use), biology.organism_classification, medicine.disease_cause, Virology, Virus, law.invention, Infectious Diseases, law, Pandemic, Multiplex polymerase chain reaction, Medicine, Immunology and Allergy, Multiplex, business, Polymerase chain reaction, Coronavirus
Abstract

Background Pandemic coronavirus disease 2019 (COVID-19) disease represents a challenge for healthcare structures. The molecular confirmation of samples from infected individuals is crucial and therefore guides public health decision making. Clusters and possibly increased diffuse transmission could occur in the context of the next influenza season. For this reason, a diagnostic test able to discriminate severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) from influenza viruses is urgently needed. Methods A multiplex real-time reverse-transcription polymerase chain reaction (PCR) assay was assessed using 1 laboratory protocol with different real-time PCR instruments. Overall, 1000 clinical samples (600 from samples SARS-CoV-2–infected patients, 200 samples from influenza-infected patients, and 200 negative samples) were analyzed. Results The assay developed was able to detect and discriminate each virus target and to intercept coinfections. The limit of quantification of each assay ranged between 5 and 10 genomic copy numbers, with a cutoff value of 37.7 and 37.8 for influenza and SARS-CoV-2 viruses, respectively. Only 2 influenza coinfections were detected in COVID-19 samples. Conclusions This study suggests that multiplex assay is a rapid, valid, and accurate method for the detection of SARS-CoV-2 and influenza viruses in clinical samples. The test may be an important diagnostic tool for both diagnostic and surveillance purposes during the seasonal influenza activity period.

Published
2020
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21. Multiplex rt-Real Time PCR assays for diagnostic testing of SARS-CoV-2 and seasonal influenza viruses. A challenge of the phase 3 pandemic setting

Author

Mancini, Fabiola, Barbanti, Fabrizio, Scaturro, Maria, Fontana, Stefano, Di Martino, Angela, Marsili, Giulia, Puzelli, Simona, Calzoletti, Laura, Facchini, Marzia, Di Mario, Giuseppina, Fabiani, Concetta, DSTAT, Antonino Bella, Riccardo, Flavia, DSTAT, Patrizio Pezzotti, Stefanelli, Paola, Rezza, Giovanni, and Ciervo, Alessandra

Subjects
multiplex Real Time PCR, SARS-CoV-2, virus diseases, COVID-19, Reproducibility of Results, Orthomyxoviridae, Sensitivity and Specificity, Diagnosis, Differential, AcademicSubjects/MED00290, ROC Curve, Area Under Curve, differential diagnosis, Influenza, Human, Major Article, Humans, RNA, Viral, Seasons, influenza viruses, Multiplex Polymerase Chain Reaction
Abstract

Pandemic coronavirus disease 2019 (COVID-19) disease represents a challenge for healthcare structures. The molecular confirmation of samples from infected individuals is crucial and therefore guides public health decision making. Clusters and possibly increased diffuse transmission could occur in the context of the next influenza season. For this reason, a diagnostic test able to discriminate severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) from influenza viruses is urgently needed.A multiplex real-time reverse-transcription polymerase chain reaction (PCR) assay was assessed using 1 laboratory protocol with different real-time PCR instruments. Overall, 1000 clinical samples (600 from samples SARS-CoV-2-infected patients, 200 samples from influenza-infected patients, and 200 negative samples) were analyzed.The assay developed was able to detect and discriminate each virus target and to intercept coinfections. The limit of quantification of each assay ranged between 5 and 10 genomic copy numbers, with a cutoff value of 37.7 and 37.8 for influenza and SARS-CoV-2 viruses, respectively. Only 2 influenza coinfections were detected in COVID-19 samples.This study suggests that multiplex assay is a rapid, valid, and accurate method for the detection of SARS-CoV-2 and influenza viruses in clinical samples. The test may be an important diagnostic tool for both diagnostic and surveillance purposes during the seasonal influenza activity period.

Published
2020

28. Characterization of Scardovia wiggsiae Biofilm by Original Scanning Electron Microscopy Protocol

Author

Bossù, Maurizio, primary, Selan, Laura, additional, Artini, Marco, additional, Relucenti, Michela, additional, Familiari, Giuseppe, additional, Papa, Rosanna, additional, Vrenna, Gianluca, additional, Spigaglia, Patrizia, additional, Barbanti, Fabrizio, additional, Salucci, Alessandro, additional, Di Giorgio, Gianni, additional, Rau, Julietta V., additional, and Polimeni, Antonella, additional

Published
2020
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32. Surface layer protein a variant of Clostridium difficile PCR-ribotype 027

Author

Spigaglia, Patrizia, Barbanti, Fabrizio, and Mastrantonio, Paola

Subjects
Gene expression -- Physiological aspects -- Genetic aspects, Bacterial proteins -- Properties -- Physiological aspects -- Genetic aspects, Genetic variation -- Identification and classification -- Genetic aspects -- Physiological aspects, Clostridium difficile -- Genetic aspects -- Physiological aspects, Health
Abstract

To the Editor: Rates and severity of Clostridium difficile infection (CDI) have recently increased worldwide and correlate with dissemination of hypervirulent epidemic strains designated PCR-ribotype 027. CDI caused by this [...]

Published
2011
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34. Multiplex Real-Time Reverse-Transcription Polymerase Chain Reaction Assays for Diagnostic Testing of Severe Acute Respiratory Syndrome Coronavirus 2 and Seasonal Influenza Viruses: A Challenge of the Phase 3 Pandemic Setting.

Author

Mancini, Fabiola, Barbanti, Fabrizio, Scaturro, Maria, Fontana, Stefano, Martino, Angela Di, Marsili, Giulia, Puzelli, Simona, Calzoletti, Laura, Facchini, Marzia, Mario, Giuseppina Di, Fabiani, Concetta, Bella, Antonino, Riccardo, Flavia, Pezzotti, Patrizio, Stefanelli, Paola, Rezza, Giovanni, Ciervo, Alessandra, Team, Istituto Superiore di Sanità (ISS) COVID-19, Di Martino, Angela, and Di Mario, Giuseppina

Subjects
DIAGNOSTIC use of polymerase chain reaction, INFLUENZA viruses, SEASONAL influenza, COVID-19, INFLUENZA, PANDEMICS
Abstract

Background: Pandemic coronavirus disease 2019 (COVID-19) disease represents a challenge for healthcare structures. The molecular confirmation of samples from infected individuals is crucial and therefore guides public health decision making. Clusters and possibly increased diffuse transmission could occur in the context of the next influenza season. For this reason, a diagnostic test able to discriminate severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) from influenza viruses is urgently needed.Methods: A multiplex real-time reverse-transcription polymerase chain reaction (PCR) assay was assessed using 1 laboratory protocol with different real-time PCR instruments. Overall, 1000 clinical samples (600 from samples SARS-CoV-2-infected patients, 200 samples from influenza-infected patients, and 200 negative samples) were analyzed.Results: The assay developed was able to detect and discriminate each virus target and to intercept coinfections. The limit of quantification of each assay ranged between 5 and 10 genomic copy numbers, with a cutoff value of 37.7 and 37.8 for influenza and SARS-CoV-2 viruses, respectively. Only 2 influenza coinfections were detected in COVID-19 samples.Conclusions: This study suggests that multiplex assay is a rapid, valid, and accurate method for the detection of SARS-CoV-2 and influenza viruses in clinical samples. The test may be an important diagnostic tool for both diagnostic and surveillance purposes during the seasonal influenza activity period. [ABSTRACT FROM AUTHOR]

Published
2021
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36. Multidrug-resistant infections in long-term care facilities: extended-spectrum β-lactamase–producing Enterobacteriaceae and hypervirulent antibiotic resistant Clostridium difficile

Author

Giufrè, Maria, primary, Accogli, Marisa, additional, Ricchizzi, Enrico, additional, Barbanti, Fabrizio, additional, Farina, Claudio, additional, Fazii, Paolo, additional, Mattei, Romano, additional, Sarti, Mario, additional, Barozzi, Agostino, additional, Buttazzi, Rossella, additional, Cosentino, Marina, additional, Nardone, Maria, additional, Savini, Vincenzo, additional, Spigaglia, Patrizia, additional, Moro, Maria Luisa, additional, and Cerquetti, Marina, additional

Published
2018
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37. Additional file 1: of Epidemiology and outcome of Clostridium difficile infections in patients hospitalized in Internal Medicine: findings from the nationwide FADOI-PRACTICE study

Author

Cioni, Giorgio, Viale, Pierluigi, Frasson, Stefania, Cipollini, Francesco, Menichetti, Francesco, Petrosillo, Nicola, Brunati, Sergio, Spigaglia, Patrizia, Vismara, Chiara, Bielli, Alessandra, Barbanti, Fabrizio, Landini, Giancarlo, Panigada, Grazia, Gussoni, Gualberto, Bonizzoni, Erminio, and Gesu, Giovanni

Abstract

List of local Ethics Committees which approved the study. (DOC 25Â kb)

Published
2016
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46. The LMW surface-layer proteins of Clostridium difficile PCR ribotypes 027 and 001 share common immunogenic properties.

Author

UCL - SSS/IREC/MBLG - Pôle de Microbiologie médicale, UCL - (SLuc) Service de microbiologie, Spigaglia, Patrizia, Galeotti, Cesira L, Barbanti, Fabrizio, Scarselli, Maria, Van Broeck, Johan, Mastrantonio, Paola, UCL - SSS/IREC/MBLG - Pôle de Microbiologie médicale, UCL - (SLuc) Service de microbiologie, Spigaglia, Patrizia, Galeotti, Cesira L, Barbanti, Fabrizio, Scarselli, Maria, Van Broeck, Johan, and Mastrantonio, Paola

Abstract

The aim of this study was to investigate the S-layer proteins (SLPs) of the hypervirulent Clostridium difficile PCR ribotype 027 and compare them with those of PCR ribotype 001 and other PCR ribotypes involved in C. difficile infection and outbreaks, by molecular analysis and immunological assays. It has been demonstrated previously that PCR ribotype 027 SlpA is conserved in C. difficile strains belonging to this PCR ribotype and that it is a new variant, showing 88 % identity with SlpA of PCR ribotype 001. As the low-molecular-weight (LMW) SLPs of C. difficile are immunodominant antigens, attention was focused on this region of the genome. Sequencing of strains of different PCR ribotypes (001, 012, 014, 017, 027 and 078) showed that SlpA was conserved among strains belonging to the same PCR ribotype. Comparison of the LMW SLP region among these strains identified ten regions with sequence identity between PCR ribotypes 027 and 001, and low conservation with the other PCR ribotypes. In particular, two of these regions corresponded to areas predicted to be surface exposed. Three specific peptides, including those of the two surface-exposed regions, were recognized by human sera against PCR ribotypes 027 and 001 and by a rabbit polyclonal serum against the SLPs of PCR ribotype 027. In contrast, these peptides were not recognized by a polyclonal serum against the SLPs of PCR ribotype 012 used as a control. These results confirm the antigenic role of the LMW SLP and suggest that it may have a role in evasion of the host immune response.

Published
2011

47. Multidrug resistance in European Clostridium difficile clinical isolates.

Author

Istituto Superiore di Sanità - Department of Infectious, FUCaM - Autre, Spigaglia, Patrizia, Barbanti, Fabrizio, Mastrantonio, Paola, Istituto Superiore di Sanità - Department of Infectious, FUCaM - Autre, Spigaglia, Patrizia, Barbanti, Fabrizio, and Mastrantonio, Paola

Abstract

Multidrug resistance and antibiotic resistance mechanisms were investigated in 316 Clostridium difficile clinical isolates collected during the first European surveillance on C. difficile in 2005.

Published
2011

48. Fluoroquinolone resistance in Clostridium difficile isolates from a prospective study of C. difficile infections in Europe.

Author

UCL - MD/MIGE - Département de microbiologie, d'immunologie et de génétique, UCL - (SLuc) Service de microbiologie, Spigaglia, Patrizia, Barbanti, Fabrizio, Mastrantonio, Paola, Brazier, Jon S, Barbut, Frédéric, Delmée, Michel, Kuijper, Ed, Poxton, Ian R, UCL - MD/MIGE - Département de microbiologie, d'immunologie et de génétique, UCL - (SLuc) Service de microbiologie, Spigaglia, Patrizia, Barbanti, Fabrizio, Mastrantonio, Paola, Brazier, Jon S, Barbut, Frédéric, Delmée, Michel, Kuijper, Ed, and Poxton, Ian R

Abstract

The European Study Group on Clostridium difficile (ESGCD) conducted a prospective study in 2005 to monitor and characterize C. difficile strains circulating in European hospitals, collecting 411 isolates. Eighty-three of these isolates, showing resistance or intermediate resistance to moxifloxacin (MX), were selected for this study to assess susceptibility to other fluoroquinolones (FQs) and to analyse the gyr genes, encoding the DNA gyrase subunits GyrA and GyrB. Twenty MX-susceptible isolates from the surveillance study were included for comparison. Overall, one amino acid substitution in GyrA (Thr82 to Ile) and four different substitutions in GyrB (Ser416 to Ala, Asp426 to Asn, Asp426 to Val and Arg447 to Lys) were identified. A high level of resistance (MIC >or=32 microg ml(-1)) to MX, ciprofloxacin (CI), gatifloxacin (GA) and levofloxacin (LE) was found in 68 isolates showing the amino acid substitution Thr82 to Ile in GyrA, in eight isolates with the substitutions Thr82 to Ile in GyrA and Ser416 to Ala in GyrB, in two isolates showing the substitution Asp426 to Asn in GyrB and in one isolate with Asp426 to Val in GyrB. The remaining four isolates showed high MICs for CI and LE, but different MIC levels for MX and GA. In particular, intermediate levels of resistance to MX were shown by two isolates, one with the substitution Thr82 to Ile in GyrA, and one showing Asp426 to Asn in GyrB. The substitution Arg447 to Lys in GyrB was found in two strains resistant to MX, CI and LE but susceptible to GA. No substitutions in GyrA were found in the FQ-susceptible strains, whereas two strains showed the amino acid change Ser416 to Ala in GyrB. Thr82 to Ile was the most frequent amino acid change identified in the C. difficile isolates examined. In contrast to previous observations, 10% of the isolates showed this substitution in association with Ser416 to Ala in GyrB. The other amino acid changes found were characteristic of a few strains belonging to certain types and/or

Published
2008

50. Epidemiology and outcome of Clostridium difficile infections in patients hospitalized in Internal Medicine: findings from the nationwide FADOI-PRACTICE study.

Author

Cioni, Giorgio, Viale, Pierluigi, Frasson, Stefania, Cipollini, Francesco, Menichetti, Francesco, Petrosillo, Nicola, Brunati, Sergio, Spigaglia, Patrizia, Vismara, Chiara, Bielli, Alessandra, Barbanti, Fabrizio, Landini, Giancarlo, Panigada, Grazia, Gussoni, Gualberto, Bonizzoni, Erminio, and Gesu, Giovanni Pietro

Subjects
CLOSTRIDIOIDES difficile, ETIOLOGY of diseases, DIARRHEA, EPIDEMIOLOGY, TREATMENT effectiveness, INTERNAL medicine, PATIENTS
Abstract

Background: Clostridium difficile (CD) is a leading cause of diarrhoea among hospitalized patients. The objective of this study was to evaluate the rate, the optimal diagnostic work-up, and outcome of CD infections (CDI) in Internal Medicine (IM) wards in Italy. Methods: PRACTICE is an observational prospective study, involving 40 IM Units and evaluating all consecutive patients hospitalized during a 4-month period. CDI were defined in case of diarrhoea when both enzyme immunoassay for GDH, and test for A/B toxin were positive. Patients with CDI were followed-up for recurrences for 4 weeks after the end of therapy. Results: Among the 10,780 patients observed, 103 (0.96 %) showed CDI, at admission or during hospitalization. A positive history for CD, antibiotics in the previous 4 weeks, recent hospitalization, female gender and age were significantly associated with CDI (multivariable analysis). In-hospital mortality was 16.5 % in CD group vs 6.7 % in No-CD group (p < 0.001), whereas median length of hospital stay was 16 (IQR = 13) vs 8 (IQR = 8) days (p < 0.001) among patients with or without CDI, respectively. Rate of CD recurrences was 14.6 %. As a post-hoc evaluation, 23 out of 34 GDH+/Tox- samples were toxin positive, when analysed by molecular method (a real-time PCR assay). The overall CD incidence rate was 5.3/10,000 patient-days. Conclusions: Our results confirm the severity of CDI in medical wards, showing high in-hospital mortality, prolonged hospitalization and frequent short-term recurrences. Further, our survey supports a 2-3 step algorithm for CD diagnosis: EIA for detecting GDH, A and B toxin, followed by a molecular method in case of toxin-negative samples. [ABSTRACT FROM AUTHOR]

Published
2016
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