Search

Your search keyword '"Barbagallo, Cristina"' showing total 157 results
Start Over Author "Barbagallo, Cristina"
157 results on '"Barbagallo, Cristina"'

2. Extracellular RNAs from Whole Urine to Distinguish Prostate Cancer from Benign Prostatic Hyperplasia.

Author

Stella, Michele, Russo, Giorgio Ivan, Leonardi, Rosario, Carcò, Daniela, Gattuso, Giuseppe, Falzone, Luca, Ferrara, Carmen, Caponnetto, Angela, Battaglia, Rosalia, Libra, Massimo, Barbagallo, Davide, Di Pietro, Cinzia, Pernagallo, Salvatore, Barbagallo, Cristina, and Ragusa, Marco

Subjects
LINCRNA, BENIGN prostatic hyperplasia, NON-coding RNA, OVERTREATMENT of cancer, MICRORNA
Abstract

RNAs, especially non-coding RNAs (ncRNAs), are crucial players in regulating cellular mechanisms due to their ability to interact with and regulate other molecules. Altered expression patterns of ncRNAs have been observed in prostate cancer (PCa), contributing to the disease's initiation, progression, and treatment response. This study aimed to evaluate the ability of a specific set of RNAs, including long ncRNAs (lncRNAs), microRNAs (miRNAs), and mRNAs, to discriminate between PCa and the non-neoplastic condition benign prostatic hyperplasia (BPH). After selecting by literature mining the most relevant RNAs differentially expressed in biofluids from PCa patients, we evaluated their discriminatory power in samples of unfiltered urine from 50 PCa and 50 BPH patients using both real-time PCR and droplet digital PCR (ddPCR). Additionally, we also optimized a protocol for urine sample manipulation and RNA extraction. This two-way validation study allowed us to establish that miRNAs (i.e., miR-27b-3p, miR-574-3p, miR-30a-5p, and miR-125b-5p) are more efficient biomarkers for PCa compared to long RNAs (mRNAs and lncRNAs) (e.g., PCA3, PCAT18, and KLK3), as their dysregulation was consistently reported in the whole urine of patients with PCa compared to those with BPH in a statistically significant manner regardless of the quantification methodology performed. Moreover, a significant increase in diagnostic performance was observed when molecular signatures composed of different miRNAs were considered. Hence, the abovementioned circulating ncRNAs represent excellent potential non-invasive biomarkers in urine capable of effectively distinguishing individuals with PCa from those with BPH, potentially reducing cancer overdiagnosis. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
View/download PDF

5. A Circular RNA Derived from the Pumilio 1 Gene Could Regulate PTEN in Human Cumulus Cells

Author

Caponnetto, Angela, primary, Ferrara, Carmen, additional, Fazzio, Anna, additional, Agosta, Noemi, additional, Scribano, Marianna, additional, Vento, Maria Elena, additional, Borzì, Placido, additional, Barbagallo, Cristina, additional, Stella, Michele, additional, Ragusa, Marco, additional, Scollo, Paolo, additional, Barbagallo, Davide, additional, Purrello, Michele, additional, Di Pietro, Cinzia, additional, and Battaglia, Rosalia, additional

Published
2024
Full Text
View/download PDF

6. Unveil Intrahepatic Cholangiocarcinoma Heterogeneity through the Lens of Omics and Multi-Omics Approaches.

Author

Porreca, Veronica, Barbagallo, Cristina, Corbella, Eleonora, Peres, Marco, Stella, Michele, Mignogna, Giuseppina, Maras, Bruno, Ragusa, Marco, and Mancone, Carmine

Subjects
*CANCER invasiveness, *CHOLANGIOCARCINOMA, *MULTIOMICS, *TUMOR markers, *INDIVIDUALIZED medicine, *TREATMENT effect heterogeneity, *MOLECULAR biology, *PATIENT satisfaction, *EARLY diagnosis
Abstract

Simple Summary: Intrahepatic cholangiocarcinoma (iCCA) is a highly aggressive primary liver cancer leading to death in 10–15% of cases. In recent years, the iCCA worldwide incidence has been increasing, and research is currently focused on identifying valuable diagnostic biomarkers and developing specific therapies to counteract the disease despite its high heterogeneity. The use of cutting-edge and broad-spectrum applications like omics represents a successful approach to studying the intricate pathobiology of iCCA. In this review, we discuss how both single- and multi-omics studies can pave the way for identifying potential biomarkers, and more importantly, for stratifying iCCA patients. This stratification aims to enhance the therapeutic intervention through personalized medicine (PM). Intrahepatic cholangiocarcinoma (iCCA) is recognized worldwide as the second leading cause of morbidity and mortality among primary liver cancers, showing a continuously increasing incidence rate in recent years. iCCA aggressiveness is revealed through its rapid and silent intrahepatic expansion and spread through the lymphatic system leading to late diagnosis and poor prognoses. Multi-omics studies have aggregated information derived from single-omics data, providing a more comprehensive understanding of the phenomena being studied. These approaches are gradually becoming powerful tools for investigating the intricate pathobiology of iCCA, facilitating the correlation between molecular signature and phenotypic manifestation. Consequently, preliminary stratifications of iCCA patients have been proposed according to their "omics" features opening the possibility of identifying potential biomarkers for early diagnosis and developing new therapies based on personalized medicine (PM). The focus of this review is to provide new and advanced insight into the molecular pathobiology of the iCCA, starting from single- to the latest multi-omics approaches, paving the way for translating new basic research into therapeutic practices. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
View/download PDF

7. A Systematic Review and Meta-Analysis of microRNA Profiling Studies in Chronic Kidney Diseases.

Author

Garmaa, Gantsetseg, Bunduc, Stefania, Kói, Tamás, Hegyi, Péter, Csupor, Dezső, Ganbat, Dariimaa, Dembrovszky, Fanni, Meznerics, Fanni Adél, Nasirzadeh, Ailar, Barbagallo, Cristina, and Kökény, Gábor

Subjects
CHRONIC kidney failure, MICRORNA, KIDNEY diseases, LUPUS nephritis, EPITHELIAL-mesenchymal transition
Abstract

Chronic kidney disease (CKD) represents an increasing health burden. Evidence suggests the importance of miRNA in diagnosing CKD, yet the reports are inconsistent. This study aimed to determine novel miRNA biomarkers and potential therapeutic targets from hypothesis-free miRNA profiling studies in human and murine CKDs. Comprehensive literature searches were conducted on five databases. Subgroup analyses of kidney diseases, sample types, disease stages, and species were conducted. A total of 38 human and 12 murine eligible studies were analyzed using Robust Rank Aggregation (RRA) and vote-counting analyses. Gene set enrichment analyses of miRNA signatures in each kidney disease were conducted using DIANA-miRPath v4.0 and MIENTURNET. As a result, top target genes, Gene Ontology terms, the interaction network between miRNA and target genes, and molecular pathways in each kidney disease were identified. According to vote-counting analysis, 145 miRNAs were dysregulated in human kidney diseases, and 32 were dysregulated in murine CKD models. By RRA, miR-26a-5p was significantly reduced in the kidney tissue of Lupus nephritis (LN), while miR-107 was decreased in LN patients' blood samples. In both species, epithelial-mesenchymal transition, Notch, mTOR signaling, apoptosis, G2/M checkpoint, and hypoxia were the most enriched pathways. These miRNA signatures and their target genes must be validated in large patient cohort studies. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
View/download PDF

11. An Uncharacterised lncRNA Coded by the ASAP1 Locus Is Downregulated in Serum of Type 2 Diabetes Mellitus Patients

Author

Barbagallo, Cristina, primary, Stella, Michele, additional, Di Mauro, Stefania, additional, Scamporrino, Alessandra, additional, Filippello, Agnese, additional, Scionti, Francesca, additional, Di Martino, Maria Teresa, additional, Purrello, Michele, additional, Ragusa, Marco, additional, Purrello, Francesco, additional, and Piro, Salvatore, additional

Published
2023
Full Text
View/download PDF

13. Er:Y2O3 and Nd:Y2O3 Nanoparticles: Synthesis, Pegylation, Characterization and Study of Their Luminescence Properties

Author

Chiechio, Regina Maria, primary, Battaglia, Rosalia, additional, Caponnetto, Angela, additional, Butera, Ester, additional, Franzò, Giorgia, additional, Reitano, Riccardo, additional, Purrello, Michele, additional, Ragusa, Marco, additional, Barbagallo, Davide, additional, Barbagallo, Cristina, additional, Di Pietro, Cinzia, additional, Marchi, Valérie, additional, Lo Faro, Maria José, additional, Contino, Annalinda, additional, Maccarrone, Giuseppe, additional, and Musumeci, Paolo, additional

Published
2022
Full Text
View/download PDF

14. circSMARCA5 Is an Upstream Regulator of the Expression of miR-126-3p, miR-515-5p, and Their mRNA Targets, Insulin-like Growth Factor Binding Protein 2 (IGFBP2) and NRAS Proto-Oncogene, GTPase (NRAS) in Glioblastoma

Author

Merulla, Aurora Eliana, primary, Stella, Michele, additional, Barbagallo, Cristina, additional, Battaglia, Rosalia, additional, Caponnetto, Angela, additional, Broggi, Giuseppe, additional, Altieri, Roberto, additional, Certo, Francesco, additional, Caltabiano, Rosario, additional, Ragusa, Marco, additional, Barbagallo, Giuseppe Maria Vincenzo, additional, Di Pietro, Cinzia, additional, Purrello, Michele, additional, and Barbagallo, Davide, additional

Published
2022
Full Text
View/download PDF

16. Er:Y 2 O 3 and Nd:Y 2 O 3 Nanoparticles: Synthesis, Pegylation, Characterization and Study of Their Luminescence Properties.

Author

Chiechio, Regina Maria, Battaglia, Rosalia, Caponnetto, Angela, Butera, Ester, Franzò, Giorgia, Reitano, Riccardo, Purrello, Michele, Ragusa, Marco, Barbagallo, Davide, Barbagallo, Cristina, Di Pietro, Cinzia, Marchi, Valérie, Lo Faro, Maria José, Contino, Annalinda, Maccarrone, Giuseppe, and Musumeci, Paolo

Subjects
LUMINESCENCE, YTTRIUM oxides, PHOTOLUMINESCENCE measurement, NANOPARTICLES, PHOTON upconversion
Abstract

Lanthanide-doped yttrium oxide nanoparticles can display selective upconversion properties, rendering them invaluable in the field of nanomedicine for both sensing and diagnostics. Different syntheses of Er:Y2O3 and Nd:Y2O3 nanoparticles (NPs) were studied and optimized to obtain small particles of regular shape and good crystallinity. The morphological and compositional characterizations of the nanoparticles were obtained with different techniques and showed that both Er:Y2O3 and Nd:Y2O3 NPs were well dispersed, with dimensions of the order of a few tens of nanometers. The photoluminescence and cathodoluminescence measurements showed that both Er:Y2O3 and Nd:Y2O3 NPs had good emission as well as upconversion. The nanophosphors were functionalized by a pegylation procedure to suppress unwanted reactions of the NPs with other biological components, making the NP systems biocompatible and the NPs soluble in water and well dispersed. The pegylated core/shell nanoparticles showed the same morphological and optical characteristics as the core, promoting their strategic role as photoactive material for theragnostics and biosensing. [ABSTRACT FROM AUTHOR]

Published
2023
Full Text
View/download PDF

19. Serum Extracellular Vesicle-Derived circHIPK3 and circSMARCA5 Are Two Novel Diagnostic Biomarkers for Glioblastoma Multiforme

Author

Stella, Michele, primary, Falzone, Luca, additional, Caponnetto, Angela, additional, Gattuso, Giuseppe, additional, Barbagallo, Cristina, additional, Battaglia, Rosalia, additional, Mirabella, Federica, additional, Broggi, Giuseppe, additional, Altieri, Roberto, additional, Certo, Francesco, additional, Caltabiano, Rosario, additional, Barbagallo, Giuseppe Maria Vincenzo, additional, Musumeci, Paolo, additional, Ragusa, Marco, additional, Pietro, Cinzia Di, additional, Libra, Massimo, additional, Purrello, Michele, additional, and Barbagallo, Davide, additional

Published
2021
Full Text
View/download PDF

21. The GAUGAA Motif Is Responsible for the Binding between circSMARCA5 and SRSF1 and Related Downstream Effects on Glioblastoma Multiforme Cell Migration and Angiogenic Potential

Author

Barbagallo, Davide, primary, Caponnetto, Angela, additional, Barbagallo, Cristina, additional, Battaglia, Rosalia, additional, Mirabella, Federica, additional, Brex, Duilia, additional, Stella, Michele, additional, Broggi, Giuseppe, additional, Altieri, Roberto, additional, Certo, Francesco, additional, Caltabiano, Rosario, additional, Barbagallo, Giuseppe Maria Vincenzo, additional, Anfuso, Carmelina Daniela, additional, Lupo, Gabriella, additional, Ragusa, Marco, additional, Di Pietro, Cinzia, additional, Hansen, Thomas Birkballe, additional, and Purrello, Michele, additional

Published
2021
Full Text
View/download PDF

22. LINC00483 Has a Potential Tumor-Suppressor Role in Colorectal Cancer Through Multiple Molecular Axes

Author

Brex, Duilia, primary, Barbagallo, Cristina, additional, Mirabella, Federica, additional, Caponnetto, Angela, additional, Battaglia, Rosalia, additional, Barbagallo, Davide, additional, Caltabiano, Rosario, additional, Broggi, Giuseppe, additional, Memeo, Lorenzo, additional, Di Pietro, Cinzia, additional, Purrello, Michele, additional, and Ragusa, Marco, additional

Published
2021
Full Text
View/download PDF

23. LncRNA LINC00518 Acts as an Oncogene in Uveal Melanoma by Regulating an RNA-Based Network

Author

Barbagallo, Cristina, primary, Caltabiano, Rosario, additional, Broggi, Giuseppe, additional, Russo, Andrea, additional, Puzzo, Lidia, additional, Avitabile, Teresio, additional, Longo, Antonio, additional, Reibaldi, Michele, additional, Barbagallo, Davide, additional, Di Pietro, Cinzia, additional, Purrello, Michele, additional, and Ragusa, Marco, additional

Published
2020
Full Text
View/download PDF

24. Histopathology and Genetic Biomarkers of Choroidal Melanoma

Author

Broggi, Giuseppe, primary, Russo, Andrea, additional, Reibaldi, Michele, additional, Russo, Daniela, additional, Varricchio, Silvia, additional, Bonfiglio, Vincenza, additional, Spatola, Corrado, additional, Barbagallo, Cristina, additional, Foti, Pietro Valerio, additional, Avitabile, Teresio, additional, Longo, Antonio, additional, and Caltabiano, Rosario, additional

Published
2020
Full Text
View/download PDF

25. Uncharacterized RNAs in Plasma of Alzheimer’s Patients Are Associated with Cognitive Impairment and Show a Potential Diagnostic Power

Author

Barbagallo, Cristina, primary, Di Martino, Maria Teresa, additional, Grasso, Margherita, additional, Salluzzo, Maria Grazia, additional, Scionti, Francesca, additional, Cosentino, Filomena Irene Ilaria, additional, Caruso, Giuseppe, additional, Barbagallo, Davide, additional, Di Pietro, Cinzia, additional, Ferri, Raffaele, additional, Caraci, Filippo, additional, Purrello, Michele, additional, and Ragusa, Marco, additional

Published
2020
Full Text
View/download PDF

26. Potential Associations Among Alteration of Salivary miRNAs, Saliva Microbiome Structure, and Cognitive Impairments in Autistic Children

Author

Ragusa, Marco, primary, Santagati, Maria, additional, Mirabella, Federica, additional, Lauretta, Giovanni, additional, Cirnigliaro, Matilde, additional, Brex, Duilia, additional, Barbagallo, Cristina, additional, Domini, Carla Noemi, additional, Gulisano, Mariangela, additional, Barone, Rita, additional, Trovato, Laura, additional, Oliveri, Salvatore, additional, Mongelli, Gino, additional, Spitale, Ambra, additional, Barbagallo, Davide, additional, Di Pietro, Cinzia, additional, Stefani, Stefania, additional, Rizzo, Renata, additional, and Purrello, Michele, additional

Published
2020
Full Text
View/download PDF

27. Circulating microRNAs Profile in Patients With Transthyretin Variant Amyloidosis

Author

Vita, Gian Luca, primary, Aguennouz, M’Hammed, additional, Polito, Francesca, additional, Oteri, Rosaria, additional, Russo, Massimo, additional, Gentile, Luca, additional, Barbagallo, Cristina, additional, Ragusa, Marco, additional, Rodolico, Carmelo, additional, Di Giorgio, Rosa Maria, additional, Toscano, Antonio, additional, Vita, Giuseppe, additional, and Mazzeo, Anna, additional

Published
2020
Full Text
View/download PDF

28. MicroRNAs in the Vitreous Humor of Patients with Retinal Detachment and a Different Grading of Proliferative Vitreoretinopathy: A Pilot Study

Author

Toro, Mario Damiano, primary, Reibaldi, Michele, additional, Avitabile, Teresio, additional, Bucolo, Claudio, additional, Salomone, Salvatore, additional, Rejdak, Robert, additional, Nowomiejska, Katarzyna, additional, Tripodi, Sarah, additional, Posarelli, Chiara, additional, Ragusa, Marco, additional, and Barbagallo, Cristina, additional

Published
2020
Full Text
View/download PDF

29. Salivary MicroRNAs: Diagnostic Markers of Mild Traumatic Brain Injury in Contact-Sport

Author

Di Pietro, Valentina, Porto, Edoardo, Ragusa, Marco, Barbagallo, Cristina, Davies, David, Forcione, Mario, Logan, Ann, Di Pietro, Cinzia, Purrello, Michele, Grey, Michael, Hammond, Douglas, Sawlani, Vijay, Barbey, Aron K., and Belli, Antonio

Subjects
Cellular and Molecular Neuroscience, saliva, microRNA, B140, Biomarkers, Concussion, MicroRNA, Mild traumatic brain injury, Saliva, Molecular Biology, mild traumatic brain injury, concussion, biomarkers, Neuroscience, Original Research
Abstract

Concussion is difficult to diagnose, particularly when symptoms are atypical or late in presenting. An accurate and timely initial assessment is crucial for clinical management. Cerebral spinal fluid (CSF) and blood markers of traumatic brain injury show promising results but their clinical applicability in concussion has significant limitations. In the study, we explored saliva as a new source of biomarkers of concussion. Saliva samples of concussed players were collected after 48–72 h from concussion and analyzed by high-throughput technologies. A discovery group of 10 concussed rugby professional and semiprofessional athletes and 10 non-concussed matched controls was used for the analysis of 92 inflammatory proteins by the Proseek-Multiplex-Inflammation technology. In addition, saliva samples of 6 concussed and 6 non-concussed athletes were used to screen 800 human microRNAs (miRNAs) by the Nanostring Technology. The results were then validated by RT-qPCR in an enlarged cohort (validation group) comprising 22 concussed athletes. Results showed, no significant variations of the 65 inflammatory proteins detected in saliva between groups but 5 microRNAs, miR-27b-3p (p = 0.016), let-7i-5p (p = 0.001), miR-142-3p (p = 0.008), miR-107 (p = 0.028), miR-135b-5p (p = 0.017) significantly upregulated in concussed athletes. Univariate ROC curve analysis showed that the differentially expressed miRNAs could be considered good classifiers of concussion. Further analyses showed significant correlation between these microRNAs and Reaction Time component of the ImPACT concussion assessment tool. In addition, biocomputation analysis predicted the involvement of these microRNAs in important biological processes that might be related to trauma, such as response to hypoxia, cell death, neurogenesis, axon repair and myelination. Ease of access and non-invasiveness of saliva samples make these biomarkers particularly suitable for concussion assessment.

Published
2018

30. LncRNA and circRNA expression profiles in tissues and serum exosomes of colorectal cancer patients and cell lines

Author

Barbagallo, Cristina

Subjects
Area 06 - Scienze mediche, CRC, long non-coding RNAs, circular RNAs, exosomes, diagnosis, molecular basis, circulating ncRNAs
Abstract

In the last few years several studies demonstrated the fundamental role of non-coding RNAs (ncRNAs) in tumor onset and progression. While the involvement and the mechanism of action of microRNAs (miRNAs) have been widely investigated, little is known about long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs), which seem to act through a plethora of molecular mechanisms regulating essential biological processes, such as cell cycle, splicing, chromatin remodeling, apoptosis, adhesion and migration. LncRNAs are non-coding RNAs longer than 200 nucleotides, with or without 5 -cap and poly(A) tail; circRNAs are circular molecules lacking free ends and thus resistant to exonucleasic degradation, transcribed from protein-coding genes. Several evidences showed that aberrant expression of lncRNAs and circRNAs is associated with various tumors, including CRC. In our study we analysed through Real-Time PCR the expression of a set of 17 lncRNAs and 31 circRNAs selected from literature in 20 CRC tissues compared to normal adjacent tissues (NATs), and in serum exosomes of 20 CRC patients compared to 20 healthy individuals. We identified 8 ncRNAs (CCAT1, CCAT2, CDR1AS, HOTAIR, MALAT1, TUG1, UCA1 and ZEB2AS1) differentially expressed (DE) in tissues and 3 ncRNAs (circ16, TUG1 and UCA1) DE in serum exosomes. Through gene ontology analysis we verified the involvement of DE ncRNAs in pathways associated with tumor progression; we also evaluated the diagnostic accuracy of ncRNAs deregulated in serum exosomes through ROC curves, suggesting their possible application in CRC non-invasive diagnosis. We observed that inhibition of MAPK pathway, associated with CRC, altered the expression of HOTAIR, MALAT1, TUG1 and UCA1 in HCT-116 colon cancer cells treated with U0126 (MEK1/2 specific inhibitor) compared to untreated cells, suggesting a possible connection between ncRNAs and MAPKs. Also, we performed a computational analysis to identify mRNAs and miRNAs involved in CRC and characterized by correlation of expression and sequence complementarity with ncRNAs deregulated in CRC tissues. Finally, we performed in vitro functional assays by silencing UCA1 expression through ASOs in HCT-116 cells and analysed the expression of its mRNA and miRNA targets, aiming to investigate the eventual degradation triggered by the interaction between ncRNAs and their miRNA/mRNA targets. Our study provide new data about aberrant expression of ncRNAs in CRC and their involvement in carcinogenesis; further analyses will be necessary to fully understand ncRNA molecular mechanisms and to evaluate their possible application in CRC diagnosis and therapy.

Published
2018

31. Specific Signatures of Serum miRNAs as Potential Biomarkers to Discriminate Clinically Similar Neurodegenerative and Vascular-Related Diseases

Author

Barbagallo, Cristina, primary, Mostile, Giovanni, additional, Baglieri, Gloriangela, additional, Giunta, Flavia, additional, Luca, Antonina, additional, Raciti, Loredana, additional, Zappia, Mario, additional, Purrello, Michele, additional, Ragusa, Marco, additional, and Nicoletti, Alessandra, additional

Published
2019
Full Text
View/download PDF

33. Upregulated microRNAs in membranous glomerulonephropathy are associated with significant downregulation of IL6 and MYC mRNAs

Author

Barbagallo, C, Passanisi, R, Mirabella, F, Cirnigliaro, M, Costanzo, A, Lauretta, G, Barbagallo, D, Bianchi, C, Pagni, F, Castorina, S, Granata, A, Di Pietro, C, Ragusa, M, Malatino, L, Purrello, M, Barbagallo, Cristina, Passanisi, Roberta, Mirabella, Federica, Cirnigliaro, Matilde, Costanzo, Arianna, Lauretta, Giovanni, Barbagallo, Davide, Bianchi, Cristina, Pagni, Fabio, Castorina, Sergio, Granata, Antonio, Di Pietro, Cinzia, Ragusa, Marco, Malatino, Lorenzo S, Purrello, Michele, Barbagallo, C, Passanisi, R, Mirabella, F, Cirnigliaro, M, Costanzo, A, Lauretta, G, Barbagallo, D, Bianchi, C, Pagni, F, Castorina, S, Granata, A, Di Pietro, C, Ragusa, M, Malatino, L, Purrello, M, Barbagallo, Cristina, Passanisi, Roberta, Mirabella, Federica, Cirnigliaro, Matilde, Costanzo, Arianna, Lauretta, Giovanni, Barbagallo, Davide, Bianchi, Cristina, Pagni, Fabio, Castorina, Sergio, Granata, Antonio, Di Pietro, Cinzia, Ragusa, Marco, Malatino, Lorenzo S, and Purrello, Michele

Abstract

Membranous glomerulonephropathy (MGN) is a glomerulopathy characterized by subepithelial deposits of immune complexes on the extracapillary side of the glomerular basement membrane. Insertion of C5b-9 (complement membrane-attack complex) into the membrane leads to functional impairment of the glomerular capillary wall. Knowledge of the molecular pathogenesis of MGN is actually scanty. MicroRNA (miRNA) profiling in MGN and unaffected tissues was performed by TaqMan Low-Density Arrays. Expression of miRNAs and miRNA targets was evaluated in Real-Time polymerase chain reaction (PCR). In vitro transient silencing of miRNAs was achieved through transfection with miRNA inhibitors. Ten miRNAs (let-7a-5p, let-7b-5p, let-7c-5p, let-7d-5p, miR-107, miR-129-3p, miR-423-5p, miR-516-3p, miR-532-3p, and miR-1275) were differentially expressed (DE) in MGN biopsies compared to unaffected controls. Interleukin 6 (IL6) and MYC messenger RNAs (mRNAs; targets of DE miRNAs) were significantly downregulated in biopsies from MGN patients, and upregulated in A498 cells following let-7a-5p or let-7c-5p transient silencing. Gene ontology analysis showed that DE miRNAs regulate pathways associated with MGN pathogenesis, including cell cycle, proliferation, and apoptosis. A significant correlation between DE miRNAs and mRNAs and clinical parameters (i.e., antiphospholipid antibodies, serum creatinine, estimated glomerular filtration, proteinuria, and serum cholesterol) has been detected. Based on our data, we propose that DE miRNAs and their downstream network may be involved in MGN pathogenesis and could be considered as potential diagnostic biomarkers of MGN

Published
2019

34. CircSMARCA5 Regulates VEGFA mRNA Splicing and Angiogenesis in Glioblastoma Multiforme Through the Binding of SRSF1

Author

Barbagallo, Davide, primary, Caponnetto, Angela, additional, Brex, Duilia, additional, Mirabella, Federica, additional, Barbagallo, Cristina, additional, Lauretta, Giovanni, additional, Morrone, Antonio, additional, Certo, Francesco, additional, Broggi, Giuseppe, additional, Caltabiano, Rosario, additional, Barbagallo, Giuseppe, additional, Spina-Purrello, Vittoria, additional, Ragusa, Marco, additional, Di Pietro, Cinzia, additional, Hansen, Thomas, additional, and Purrello, Michele, additional

Published
2019
Full Text
View/download PDF

35. Upregulated microRNAs in membranous glomerulonephropathy are associated with significant downregulation of IL6 and MYC mRNAs

Author

Barbagallo, Cristina, primary, Passanisi, Roberta, additional, Mirabella, Federica, additional, Cirnigliaro, Matilde, additional, Costanzo, Arianna, additional, Lauretta, Giovanni, additional, Barbagallo, Davide, additional, Bianchi, Cristina, additional, Pagni, Fabio, additional, Castorina, Sergio, additional, Granata, Antonio, additional, Di Pietro, Cinzia, additional, Ragusa, Marco, additional, Malatino, Lorenzo S., additional, and Purrello, Michele, additional

Published
2018
Full Text
View/download PDF

36. Molecular Crosstalking among Noncoding RNAs: A New Network Layer of Genome Regulation in Cancer

Author

Ragusa, Marco, Barbagallo, Cristina, Brex, Duilia, Caponnetto, Angela, Cirnigliaro, Matilde, Battaglia, Rosalia, Barbagallo, Davide, Di Pietro, Cinzia, and Purrello, Michele

Subjects
Article Subject
Abstract

Over the past few years, noncoding RNAs (ncRNAs) have been extensively studied because of the significant biological roles that they play in regulation of cellular mechanisms. ncRNAs are associated to higher eukaryotes complexity; accordingly, their dysfunction results in pathological phenotypes, including cancer. To date, most research efforts have been mainly focused on how ncRNAs could modulate the expression of protein-coding genes in pathological phenotypes. However, recent evidence has shown the existence of an unexpected interplay among ncRNAs that strongly influences cancer development and progression. ncRNAs can interact with and regulate each other through various molecular mechanisms generating a complex network including different species of RNAs (e.g., mRNAs, miRNAs, lncRNAs, and circRNAs). Such a hidden network of RNA-RNA competitive interactions pervades and modulates the physiological functioning of canonical protein-coding pathways involved in proliferation, differentiation, and metastasis in cancer. Moreover, the pivotal role of ncRNAs as keystones of network structural integrity makes them very attractive and promising targets for innovative RNA-based therapeutics. In this review we will discuss: (1) the current knowledge on complex crosstalk among ncRNAs, with a special focus on cancer; and (2) the main issues and criticisms concerning ncRNAs targeting in therapeutics.

Published
2017
Full Text
View/download PDF

37. CircSMARCA5 Inhibits Migration of Glioblastoma Multiforme Cells by Regulating a Molecular Axis Involving Splicing Factors SRSF1/SRSF3/PTB

Author

Barbagallo, Davide, primary, Caponnetto, Angela, additional, Cirnigliaro, Matilde, additional, Brex, Duilia, additional, Barbagallo, Cristina, additional, D’Angeli, Floriana, additional, Morrone, Antonio, additional, Caltabiano, Rosario, additional, Barbagallo, Giuseppe, additional, Ragusa, Marco, additional, Di Pietro, Cinzia, additional, Hansen, Thomas, additional, and Purrello, Michele, additional

Published
2018
Full Text
View/download PDF

40. Correction: miRNAs in the vitreous humor of patients affected by idiopathic epiretinal membrane and macular hole

Author

Russo, Andrea, primary, Ragusa, Marco, additional, Barbagallo, Cristina, additional, Longo, Antonio, additional, Avitabile, Teresio, additional, Uva, Maurizio G., additional, Bonfiglio, Vincenza, additional, Toro, Mario D., additional, Caltabiano, Rosario, additional, Mariotti, Cesare, additional, Boscia, Francesco, additional, Romano, Mario R., additional, Di Pietro, Cinzia, additional, Barbagallo, Davide, additional, Purrello, Michele, additional, and Reibaldi, Michele, additional

Published
2017
Full Text
View/download PDF

41. Retinal and Circulating miRNAs in Age-Related Macular Degeneration: An In vivo Animal and Human Study

Author

Romano, Giovanni L., primary, Platania, Chiara B. M., additional, Drago, Filippo, additional, Salomone, Salvatore, additional, Ragusa, Marco, additional, Barbagallo, Cristina, additional, Di Pietro, Cinzia, additional, Purrello, Michele, additional, Reibaldi, Michele, additional, Avitabile, Teresio, additional, Longo, Antonio, additional, and Bucolo, Claudio, additional

Published
2017
Full Text
View/download PDF

42. miRNAs in the vitreous humor of patients affected by idiopathic epiretinal membrane and macular hole

Author

Russo, Andrea, primary, Ragusa, Marco, additional, Barbagallo, Cristina, additional, Longo, Antonio, additional, Avitabile, Teresio, additional, Uva, Maurizio G., additional, Bonfiglio, Vincenza, additional, Toro, Mario D., additional, Caltabiano, Rosario, additional, Mariotti, Cesare, additional, Boscia, Francesco, additional, Romano, Mario, additional, Di Pietro, Cinzia, additional, Barbagallo, Davide, additional, Purrello, Michele, additional, and Reibaldi, Michele, additional

Published
2017
Full Text
View/download PDF

43. Upregulated microRNAs in membranous glomerulonephropathy are associated with significant downregulation of IL6 and MYC mRNAs.

Author

Barbagallo, Cristina, Passanisi, Roberta, Mirabella, Federica, Cirnigliaro, Matilde, Costanzo, Arianna, Lauretta, Giovanni, Barbagallo, Davide, Bianchi, Cristina, Pagni, Fabio, Castorina, Sergio, Granata, Antonio, Di Pietro, Cinzia, Ragusa, Marco, Malatino, Lorenzo S., and Purrello, Michele

Subjects
*MESSENGER RNA, *PHOSPHOLIPID antibodies, *BLOOD cholesterol, *POLYMERASE chain reaction, *IMMUNE complexes, *BASAL lamina
Abstract

Membranous glomerulonephropathy (MGN) is a glomerulopathy characterized by subepithelial deposits of immune complexes on the extracapillary side of the glomerular basement membrane. Insertion of C5b‐9 (complement membrane‐attack complex) into the membrane leads to functional impairment of the glomerular capillary wall. Knowledge of the molecular pathogenesis of MGN is actually scanty. MicroRNA (miRNA) profiling in MGN and unaffected tissues was performed by TaqMan Low‐Density Arrays. Expression of miRNAs and miRNA targets was evaluated in Real‐Time polymerase chain reaction (PCR). In vitro transient silencing of miRNAs was achieved through transfection with miRNA inhibitors. Ten miRNAs (let‐7a‐5p, let‐7b‐5p, let‐7c‐5p, let‐7d‐5p, miR‐107, miR‐129‐3p, miR‐423‐5p, miR‐516‐3p, miR‐532‐3p, and miR‐1275) were differentially expressed (DE) in MGN biopsies compared to unaffected controls. Interleukin 6 (IL6) and MYC messenger RNAs (mRNAs; targets of DE miRNAs) were significantly downregulated in biopsies from MGN patients, and upregulated in A498 cells following let‐7a‐5p or let‐7c‐5p transient silencing. Gene ontology analysis showed that DE miRNAs regulate pathways associated with MGN pathogenesis, including cell cycle, proliferation, and apoptosis. A significant correlation between DE miRNAs and mRNAs and clinical parameters (i.e., antiphospholipid antibodies, serum creatinine, estimated glomerular filtration, proteinuria, and serum cholesterol) has been detected. Based on our data, we propose that DE miRNAs and their downstream network may be involved in MGN pathogenesis and could be considered as potential diagnostic biomarkers of MGN. [ABSTRACT FROM AUTHOR]

Published
2019
Full Text
View/download PDF

45. miRNAs Plasma Profiles in Vascular Dementia: Biomolecular Data and Biomedical Implications

Author

Ragusa, Marco, primary, Bosco, Paolo, additional, Tamburello, Lucia, additional, Barbagallo, Cristina, additional, Condorelli, Angelo G., additional, Tornitore, Mariangela, additional, Spada, Rosario S., additional, Barbagallo, Davide, additional, Scalia, Marina, additional, Elia, Maurizio, additional, Di Pietro, Cinzia, additional, and Purrello, Michele, additional

Published
2016
Full Text
View/download PDF

46. Circulating miRNAs profiles in tourette syndrome: molecular data and clinical implications

Author

Rizzo, Renata, primary, Ragusa, Marco, additional, Barbagallo, Cristina, additional, Sammito, Mariangela, additional, Gulisano, Mariangela, additional, Calì, Paola V, additional, Pappalardo, Claudio, additional, Barchitta, Martina, additional, Granata, Mariagrazia, additional, Condorelli, Angelo G, additional, Barbagallo, Davide, additional, Scalia, Marina, additional, Agodi, Antonella, additional, Di Pietro, Cinzia, additional, and Purrello, Michele, additional

Published
2015
Full Text
View/download PDF

47. miRNA profiling in vitreous humor, vitreal exosomes and serum from uveal melanoma patients: Pathological and diagnostic implications

Author

Ragusa, Marco, primary, Barbagallo, Cristina, additional, Statello, Luisa, additional, Caltabiano, Rosario, additional, Russo, Andrea, additional, Puzzo, Lidia, additional, Avitabile, Teresio, additional, Longo, Antonio, additional, Toro, Mario D, additional, Barbagallo, Davide, additional, Valadi, Hadi, additional, Di Pietro, Cinzia, additional, Purrello, Michele, additional, and Reibaldi, Michele, additional

Published
2015
Full Text
View/download PDF

48. Highly skewed distribution of miRNAs and proteins between colorectal cancer cells and their exosomes following Cetuximab treatment: biomolecular, genetic and translational implications

Author

Ragusa, Marco, primary, Statello, Luisa, additional, Maugeri, Marco, additional, Barbagallo, Cristina, additional, Passanisi, Roberta, additional, Alhamdani, Mohamed S., additional, Li Destri, Giovanni, additional, Cappellani, Alessandro, additional, Barbagallo, Davide, additional, Scalia, Marina, additional, Valadi, Hadi, additional, Hoheisel, Jörg D., additional, Di Pietro, Cinzia, additional, and Purrello, Michele, additional

Published
2014
Full Text
View/download PDF

49. MicroRNAs in seminal plasma are able to discern infertile men at increased risk of developing testicular cancer.

Author

Ferrara, Carmen, Battaglia, Rosalia, Caponnetto, Angela, Fazzio, Anna, Stella, Michele, Barbagallo, Cristina, Musso, Nicolò, Lunelio, Federica, Vento, Maria Elena, Borzì, Placido, Scollo, Paolo, Barbagallo, Davide, Ragusa, Marco, Pernagallo, Salvatore, and Di Pietro, Cinzia

Subjects
*GERM cell tumors, *PLASMA potentials, *RECEIVER operating characteristic curves, *TESTICULAR cancer, *DISEASE risk factors
Abstract

Male infertility is a risk factor for the development of testicular germ cell tumors. In this study, we investigated microRNA profiles in seminal plasma to identify potential noninvasive biomarkers able to discriminate the men at highest risk of developing cancer among the infertile population. We compared the microRNA profiles of individuals affected by testicular germ cell tumors and healthy individuals with normal or impaired spermiograms using high‐throughput technology and confirmed the results by single‐assay digital PCR. We found that miR‐221‐3p and miR‐222‐3p were downregulated and miR‐126‐3p was upregulated in cancer patients compared to both infertile and fertile men. ROC curve analysis confirmed that miR‐126 upregulation is able to identify cancer patients among the infertile male population. In addition, in‐depth bioinformatics analysis based on weighted gene co‐expression networks showed that the identified miRNAs regulate cellular pathways involved in cancer. [ABSTRACT FROM AUTHOR]

Published
2024
Full Text
View/download PDF

50. miRNAs in the vitreous humor of patients affected by idiopathic epiretinal membrane and macular hole

Author

Russo A, Ragusa M, Barbagallo C, Longo A, Avitabile T, Uva MG, Bonfiglio V, Toro M, Caltabiano R, Mariotti C, Boscia F, Romano MR, Di Pietro C, Barbagallo D, Purrello M, Reibaldi M, Russo, Andrea, Ragusa, Marco, Barbagallo, Cristina, Longo, Antonio, Avitabile, Teresio, Uva, Maurizio G, Bonfiglio, Vincenza, Toro, Mario D, Caltabiano, Rosario, Mariotti, Cesare, Boscia, Francesco, Romano, Mario R, Di Pietro, Cinzia, Barbagallo, Davide, Purrello, Michele, Reibaldi, Michele, Russo, A, Ragusa, M, Barbagallo, C, Longo, A, Avitabile, T, Uva, Mg, Bonfiglio, V, Toro, M, Caltabiano, R, Mariotti, C, Boscia, F, Romano, Mr, Di Pietro, C, Barbagallo, D, Purrello, M, and Reibaldi, M

Subjects
Male, Multidisciplinary, lcsh:R, lcsh:Medicine, Correction, Down-Regulation, Epiretinal Membrane, Middle Aged, Retinal Perforations, Vitreous Body, MicroRNAs, Vitrectomy, Humans, lcsh:Q, Female, Prospective Studies, lcsh:Science, Transcriptome, miRNA, macular hole
Abstract

The aim of the present study was to assess the expression of miRNAs in the Vitreous Humor (VH) of patients with Macular Hole (MH) and Epiretinal Membrane (ERM) compared to a control group.In this prospective, comparative study, 2-ml of VH was extracted from the core of the vitreous chamber in consecutive patients who underwent standard vitrectomy for ERM and MH. RNA was extracted and TaqMan® Low Density Arrays (TLDAs) were used to profile the transcriptome of 754 miRNAs. Results were validated by single TaqMan® assays. Finally, we created a biological network of differentially expressed miRNA targets and their nearest neighbors.Overall 10 eyes with MH, 16 eyes with idiopathic ERM and 6 controls were enrolled in the study. Profiling data identified 5 miRNAs differentially expressed in patients affected by MH and ERM with respect to controls. Four were downregulated (miR-19b, miR-24, miR-155, miR-451) and 1 was downregulated (miR-29a); TaqMan® assays of the VH of patients affected by MH and ERM, with respect to controls, showed that the most differentially expressed were miR-19b (FC -9.13, p:0.00004), mir-24 (FC -7.52, p:0.004) and miR-142-3p (FC -5.32, p:0.011). Our network data showed that deregulation of differentially expressed miRNAs induces an alteration of several pathways associated with genes involved in both MH and ERM.The present study suggests that disregulation of miR-19b, miR-24 and miR-142-3p, might be related to the alterations that characterize patients affected by MH and ERM.

Published
2017

Catalog

Books, media, physical & digital resources
See catalog results