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Abstract Background The mandibular retromolar space (RMS) has not been extensively studied in relation to various sagittal skeletal classes and patterns of third-molar eruption. The objective of this study was to test the null hypothesis that there is no difference in the mandibular RMS among normodivergent subjects with different skeletal classes and patterns of mandibular third-molar eruption, using cone-beam computed tomography (CBCT). Method A total of 105 normodivergent patients (20–40 years) were included in this study. Participants were categorized into Class I, II and III groups based on ANB and further impacted and erupted groups based on the eruption patterns of the mandibular third molars. Measurements of the mandibular RMS were taken at four planes parallel to the occlusal plane, along the cusp line. Comparative analyses were conducted among the three sagittal groups and between the impacted and erupted groups. Results The Class II group exhibited a statistically smaller RMS (P

Abstract Background This study aimed to investigate the relationship between phthalates exposure and estrogen and progesterone levels, as well as their role in late-onset preeclampsia. Methods A total of 60 pregnant women who met the inclusion and exclusion criteria were recruited. Based on the diagnosis of preeclampsia, participants were divided into two groups: normotensive pregnant women (n = 30) and pregnant women with late-onset preeclampsia (n = 30). The major metabolites of phthalates (MMP, MEP, MiBP, MBP, MEHP, MEOHP, MEHHP) and sex steroid hormones (estrogen and progesterone) were quantified in urine samples of the participants. Results No significant differences were observed in the levels of MMP, MEP, MiBP, MBP, MEHP, MEOHP, and MEHHP between women with preeclampsia and normotensive pregnant women (P > 0.05). The urinary estrogen showed a negative correlation with systolic blood pressure (rs= -0.46, P

Abstract The static properties and dynamic evolution of bottomonium states in a hot QCD medium are investigated through the Schrödinger equation with complex heavy quark potentials, which have been presented recently in a lattice QCD study and with three different extractions. This approach builds a direct connection between the in-medium heavy quark potentials from the lattice QCD to the experimental observables. The yields and nuclear modification factors $$R_{AA}$$ R AA of bottomonium in Pb–Pb collisions at $$\sqrt{s_\textrm{NN}}=5.02$$ s NN = 5.02 TeV are calculated in this work. Our results show a large suppression of the bottomonium yield in heavy ion collisions due to the large imaginary potential. To understand bottomonium $$R_{AA}$$ R AA based on lattice QCD potentials, we propose a formation time for bottomonium states and find that experimental data can be well explained with the heavy quark potential extracted by the Padé fit, which shows no color screening in the real part potential.

Abstract Understanding the polarization property of $$J/\psi $$ J / ψ is critical to constrain its production mechanism. In addition, the polarization of $$J/\psi $$ J / ψ can reveal the impact of strong electromagnetic and vorticity fields in relativistic heavy ion collisions. In this study, we analyzed the yield and polarization of $$J/\psi $$ J / ψ in relativistic heavy ion collisions at different centrality and transverse momentum regions, using three different reference frames: the Collins–Soper frame, the helicity frame, and the event plane frame. The polarization of initially produced $$J/\psi $$ J / ψ is determined by the NRQCD calculation and is similar to that of pp collisions. However, both unpolarization and transverse polarization are considered for the regenerated $$J/\psi $$ J / ψ . Our results indicate that the polarization at high $$p_T$$ p T is similar to that observed in pp collisions. However, at low $$p_T$$ p T , where regenerated $$J/\psi $$ J / ψ dominates, it is likely that the polarized charm quarks in the rotational QGP medium are responsible for this phenomenon. Our study supplies a baseline for future research on the effects of strong electromagnetic and vorticity fields on $$J/\psi $$ J / ψ polarization.

ObjectiveTo evaluate the effects of high-intensity interval training (HIIT) on glycolipid metabolism among type 2 diabetes patients.MethodsHIIT is consistent with an exercise program (65%-90%VO2max or 75%-95% HRmax; exercise cycle≥2 weeks; frequency ≥ 2 times/week). A meta-analysis was conducted utilizing the random effects model to synthesize the data.ResultsA total of 22 RCT studies with 1034 diabetic patients were included. Compared to moderate-intensity aerobic exercise or conventional controls, HIIT yields noteworthy effects on FBG (MD: -0.55; 95% CI: -0.85- -0.25, Hedges’ g =0.98), 2h-PG (MD: -0.36; 95% CI: -0.57- -0.14, Hedges’ g =1.05), FINS (MD: -0.41; 95% CI: -0.79- -0.03, Hedges’ g =1.07), HbA1c (MD: -0.60; 95% CI: -0.84- -0.36, Hedges’ g =2.69), TC (MD: -0.58; 95% CI: -0.80- -0.36, Hedges’ g =2.36), TG (MD: -0.50; 95% CI: -0.86- -0.14, Hedges’ g =1.50), HDL (MD: 0.62; 95% CI: 0.29–0.95, Hedges’ g =1.19) and LDL (MD: -0.31; 95% CI: -0.56- -0.08, Hedges’ g =0.91), all of the above p

Abstract Purpose The purpose of this study was to investigate the risk factors for umbilical artery thrombosis (UAT) and the relationship between umbilical artery thrombosis and perinatal outcomes. Methods This was a retrospective study that enrolled singleton pregnant women who were diagnosed with umbilical artery thrombosis. The control group recruited pregnant woman with three umbilical vessels or those with isolated single umbilical artery (iSUA) who were matched with umbilical artery thrombosis group. The risk factors and perinatal outcomes were compared between the groups. Results Preconception BMI (OR [95%CI]: 1.212 [1.038–1.416]), abnormal umbilical cord insertion (OR [95%CI]: 16.695 [1.333-209.177]) and thrombophilia (OR [95%CI]: 15.840 [1.112-223.699]) were statistically significant risk factors for umbilical artery thrombosis. An elongated prothrombin time (OR [95%CI]: 2.069[1.091–3.924]) was strongly associated with the occurrence of UAT. The risks of cesarean delivery, preterm birth, fetal growth restriction, neonatal asphyxia, and intraamniotic infection were higher in pregnancies with UAT than in pregnancies with three umbilical vessels or isolated single umbilical artery (P

We studied the photoproduction of dileptons from strong electromagnetic fields generated by the nucleus in relativistic heavy-ion collisions. The production of dileptons is calculated based on the Equivalent Photon Approximation (EPA) method, which depends on the strength of the electromagnetic fields and the density of protons in the nucleus. With the EPA method, we construct the connections between dilepton photoproduction and the electromagnetic form factors in the nucleus. Finally, the nuclear proton densities can be determined with the dilepton photoproduction, which is employed to extract the neutron skin in the nucleus. Our calculations indicate that the dilepton photoproduction varies evidently with different proton densities in the nucleus, suggesting a deeper symmetry underlying the connections between proton density (or the neutron skin) and the dilepton photoproduction. This offers a new way to study the neutron skin in the nucleus.

This paper proposes a composite model that combines convolutional neural network models and mechanical analysis to determine the forces acting on an object. First, we establish a model using Newtonian mechanics to analyze the forces experienced by the human body during movement, particularly the forces on joints. The model calculates the mapping relationship between the object’s movement and the forces on the joints. Then, by analyzing a large number of fencing competition videos using a deep learning model, we extract video features to study the torques and forces on human joints. Our analysis of numerous images reveals that, in certain movement patterns, the peak pressure on the knee joint can be two to three times higher than in a normal state, while the driving knee can withstand peak torques of 400–600 Nm. This straightforward model can effectively capture the forces and torques on the human body during movement using a deep neural network. Furthermore, this model can also be applied to problems involving non-rigid body motion.

Abstract Background The purpose of this study was to investigate the relationship between the size and duration of asymptomatic subchorionic hematoma and pregnancy outcomes in women with singleton pregnancies. Methods This was a retrospective study that enrolled 701 singleton pregnant women who were diagnosed with asymptomatic subchorionic hematoma by ultrasound at 5–10 gestational weeks. The control group recruited 640 normal pregnant women without subchorionic hematoma who were matched with subchorionic hematoma group on baseline characteristics. The pregnancy outcomes were compared between the two groups, and the associations of the size and duration of subchorionic hematoma with pregnancy outcomes were analyzed by logistic regression model. Results Compared with the normal pregnancy group, the incidence of, gestational diabetes mellitus, gestational thrombocytopenia, placenta adhesion, fetal growth restriction, macrosomia in subchorionic hematoma group were higher (all P

Abstract Background Many studies have confirmed the association of aquaporins (AQPs) with abnormal amniotic fluid volume (AFV). In our previous experiments, we found that Tanshinone IIA was able to regulate the expression of AQP1 and AQP3. However, the exact mechanism by which Tanshinone IIA regulates AQPs protein expression and its effect on AFV remains unclear. The purpose of this study was to investigate the effects of Tanshinone IIA on AFV and the possible molecular mechanism of regulation of AQP1 and AQP3. Methods The expression of AQPs protein in the amniotic membranes was compared between pregnant women with normal pregnancy and those with isolated oligohydramnios. The AQP1 knockout (AQP1-KO) mice and wild-type (WT) mice were treated with saline or Tanshinone IIA (10 mg/kg) at 13.5GD and 16.5GD. Human amniotic epithelium cells (hAECs) from pregnant women with normal AFV and isolated oligohydramnios were incubated with 35 μmmol/L Tanshinone IIA or 25 mmol/L LiCl [inhibitor of glycogen synthetic kinase 3β (GSK-3β)]. The protein expressions of AQPs, GSK-3β, phospho-GSK-3β (Ser9) in fetal membranes of mice and human amniotic epithelium cells were detected by western blotting. Results The expression of AQP1 protein in the amniotic membrane of isolated oligohydramnios was increased compared with normal pregnancy. The AFV in AQP1-KO mice is higher than that in WT mice. In wild-type mice, AFV in Tanshinone IIA group was significantly higher than that in control group, and AQP1 protein expression was significantly lower than that in control group, but in AQP1 knockout mice, Tanshinone IIA reduced amniotic fluid volume and AQP3 protein expression at 16.5GD. Tanshinone IIA reduced AQP1, AQP3 and p-GSK-3β (Ser9) protein expression in normal hAECs, and this effect was inhibited by LiCl. In hAECs with oligohydramnios, the down-regulation of AQP1 and up-regulation of AQP3 by Tanshinone IIA was independent of GSK-3β signaling pathway. Conclusions Tanshinone IIA may increase AFV in normal pregnancy by downregulating AQP1 protein expression in the fetal membranes, which may be associated with p-GSK-3β signaling pathway. But a larger AFV in AQP1-KO mice was significantly attenuated by Tanshinone IIA, which may be related to AQP3. Tanshinone IIA is a promising drug for the treatment of amniotic fluid abnormality.

Abstract In this work, we propose to search for the exotic doubly charmed meson $$T_{cc}^+$$ T cc + and its analog $$T_{cc\bar{s}}^+$$ T c c s ¯ + in $$B_c^+$$ B c + decays, which provide a good environment for the formation of the exotic state containing double charm quarks. Within the molecular scheme, the production of $$T_{cc}^+$$ T cc + and $$T_{cc\bar{s}}^+$$ T c c s ¯ + through various rescattering processes with different intermediate states are investigated. For the moderate values of model parameters, the branching ratios of $$B_c^+$$ B c + decaying into $$T_{cc}^+ \bar{D}^{0}$$ T cc + D ¯ 0 , $$T_{cc}^+ \bar{D}^{*0}$$ T cc + D ¯ ∗ 0 , $$T_{cc\bar{s}}^+ \bar{D}^{0}$$ T c c s ¯ + D ¯ 0 and $$T_{cc\bar{s}}^+ \bar{D}^{*0}$$ T c c s ¯ + D ¯ ∗ 0 are estimated to be of the order of $$10^{-7}$$ 10 - 7 , $$10^{-5}$$ 10 - 5 , $$10^{-6}$$ 10 - 6 and $$10^{-4}$$ 10 - 4 , respectively, which may be tested by future experiments.

Abstract Background With the development of China’s two-child-policy, vaginal birth after cesarean section (VBAC) has aroused public concern. It is important to understand the labour characteristics and intrapartum management of women attempting VBAC to enhance the rates of successful VBAC. The purpose of our research was to investigate the differences in the characteristics of labor, intervention measures and perinatal outcomes between women who had a VBAC and primiparas or multiparas not undergoing VBAC, providing clinical references of intrapartum management for women who are planning a VBAC. Material and methods This observational retrospective study enrolled all women who laboured spontaneously and who had a VBAC (n = 139) at the Second Affiliated Hospital of Wenzhou Medical University in China between 2016 and 2019. They were allocated into VBAC group A (the previous cesarean section was performed before dilation of the cervix) and VBAC group B (the previous cesarean section was performed after dilation of the cervix). The primipara control group included 149 primiparae, and the multipara control group included 155 multiparae with second vaginal birth. Durations of labor, intervention measures and perinatal outcomes were compared among the groups. Results The durations of labor, intrapartum interventions and maternal and neonatal outcomes in VBAC group A were similar to those of the VBAC group B. However, all women who had a VBAC and those in VBAC group A had shorter first, second and the total stages of labor than primiparae. All women with VBAC and those in VBAC group B had longer second stage of labor, but shorter third stage of labor than multiparae. Oxytocin, labor analgesia and artificial rupture of membranes were administered less often in women with VBAC than in primiparae, while phloroglucinol was administered more often in women with VBAC than in multiparae. Women who had a VBAC were more likely to receive episiotomy and had higher incidences of postpartum hemorrhage than primipara and multipara women. Conclusions Labor characteristics, intrapartum interventions and perinatal outcomes in women who had a VBAC with cervical dilation were similar to those in women who had a VBAC without cervical dilation before the previous cesarean section, but differed significantly from those of multiparae and primiparae who did not undergo VBAC.

We employ the time-dependent Schrödinger equation with different complex potentials to study the bottomonium sequential suppression in Pb-Pb collisions at sNN=2.76 TeV and 5.02 TeV and Au-Au collisions at sNN=200 GeV. Both color screening effect and the random scatterings with thermal partons are considered in the real and imaginary parts of the heavy-quark potentials. As the real part of the heavy-quark potential is between the free energy F(T,r) and the internal energy U(T,r) of heavy quarkonium, we parametrize different potentials with a function of F and U to evolve the bottomonium wave packages in the medium. We find that when the real part of the potential is close to U(T,r), it can explain well the pattern of bottomonium sequential suppression where their nuclear modification factors satisfy the relation RAA(1s)>RAA(2s)>RAA(3s) observed in experiments. In the other limit of F(T,r), bottomonium wave packages tend to expand due to weak attracted force, which results in evident transitions from ϒ(2s) to ϒ(3s) components and does not satisfy the sequential suppression pattern. We suggest that the bottomonium sequential suppression can be a probe of strong heavy-quark potential in the medium.

The diffusion coefficient of heavy quarks in a deconfined medium is examined in this research using a deep convolutional neural network (CNN) that is trained with data from relativistic heavy ion collisions involving heavy flavor hadrons. The CNN is trained using observables such as the nuclear modification factor RAA and the elliptic flow v2 of non-prompt J/ψ from the B-hadron decay in different centralities, where B meson evolutions are calculated using the Langevin equation and the instantaneous coalescence model. The CNN outputs the parameters, thereby characterizing the temperature and momentum dependence of the heavy quark diffusion coefficient. By inputting the experimental data of the non-prompt J/ψ(RAA,v2) from various collision centralities into multiple channels of a well-trained network, we derive the values of the diffusion coefficient parameters. Additionally, we evaluate the uncertainty in determining the diffusion coefficient by taking into account the uncertainties present in the experimental data (RAA,v2), which serve as inputs to the deep neural network.

Background:Brucea javanica (L.) Merr, has a long history to be an anti-dysentery medicine for thousand of years, which is commonly called “Ya-Dan-Zi” in Chinese. The common liquid preparation of its seed, B. javanica oil (BJO) exerts anti-inflammatory action in gastrointestinal diseases and is popularly used as an antitumor adjuvant in Asia. However, there is no report that BJO has the potential to treat 5-Fluorouracil (5-FU)-induced chemotherapeutic intestinal mucosal injury (CIM).Aim of the study: To test the hypothesis that BJO has potential intestinal protection on intestinal mucosal injury caused by 5-FU in mice and to explore the mechanisms.Materials and methods: Kunming mice (half male and female), were randomly divided into six groups: normal group, 5-FU group (5-FU, 60 mg/kg), LO group (loperamide, 4.0 mg/kg), BJO group (0.125, 0.25, 0.50 g/kg). CIM was induced by intraperitoneal injection of 5-FU at a dose of 60 mg/kg/day for 5 days (from day 1 to day 5). BJO and LO were given orally 30 min prior to 5-FU administration for 7 days (from day 1 to day 7). The ameliorative effects of BJO were assessed by body weight, diarrhea assessment, and H&E staining of the intestine. Furthermore, the changes in oxidative stress level, inflammatory level, intestinal epithelial cell apoptosis, and proliferation, as well as the amount of intestinal tight junction proteins were evaluated. Finally, the involvements of the Nrf2/HO-1 pathway were tested by western blot.Results: BJO effectively alleviated 5-FU-induced CIM, as represented by the improvement of body weight, diarrhea syndrome, and histopathological changes in the ileum. BJO not only attenuated oxidative stress by upregulating SOD and downregulating MDA in the serum, but also reduced the intestinal level of COX-2 and inflammatory cytokines, and repressed CXCL1/2 and NLRP3 inflammasome activation. Moreover, BJO ameliorated 5-FU-induced epithelial apoptosis as evidenced by the downregulation of Bax and caspase-3 and the upregulation of Bcl-2, but enhanced mucosal epithelial cell proliferation as implied by the increase of crypt-localized proliferating cell nuclear antigen (PCNA) level. Furthermore, BJO contributed to the mucosal barrier by raising the level of tight junction proteins (ZO-1, occludin, and claudin-1). Mechanistically, these anti-intestinal mucositis pharmacological effects of BJO were relevant for the activation of Nrf2/HO-1 in the intestinal tissues.Conclusion: The present study provides new insights into the protective effects of BJO against CIM and suggests that BJO deserves to be applied as a potential therapeutic agent for the prevention of CIM.

Cl− movement and Cl−-sensitive signal pathways contributes to the survival and switch of inflammatory phenotype of microglia and are believed to play a key role in the inflammatory brain injury after ischemic stroke. Here, we demonstrated an important role of Cl− transmembrane transporter Swell1, in the survival and M2-like polarization of microglia in ischemic stroke. Knockdown or overexpression of Swell1 in cultured microglia inhibited or increased hypotonic-activated Cl− currents, respectively, and these changes were completely blocked by the volume-regulated anion channels (VRACs) inhibitor DCPIB. Swell1 conditional knock-in mice promoted microglia survival in ischemic brain region and resulted in significant reductions in neural cell death, infarction volume and neurological deficits following transient middle cerebral artery occlusion (tMCAO). Using gene manipulating technique and pharmacological inhibitors, we further revealed that Swell1 opening led to SGK1 (a Cl−-sensitive kinase)-mediated activation of FOXO3a/CREB as well as WNK1 (another Cl−-sensitive kinase)-mediated SPAK/OSR1-CCCs activation, which promoted microglia survival and M2-like polarization, thereby attenuating neuroinflammation and ischemic brain injury. Taken together, our results demonstrated that Swell1 is an essential component of microglia VRACs and its activation protects against ischemic brain injury through promoting microglia survival and M2-like polarization.

Abstract Background As a prevalent type of cryptogenic fibrotic disease with high mortality, idiopathic pulmonary fibrosis (IPF) still lacks effective therapeutic drugs. The compounds extracted from buds and flowers of Chrysanthemum indicum Linné with supercritical-carbon dioxide fluid (CISCFE) has been confirmed to have antioxidant, anti-inflammatory, and lung-protective effects. This paper aimed to clarify whether CISCFE could treat IPF induced by bleomycin (BLM) and elucidate the related mechanisms. Methods Rats (Sprague-Dawley, male) were separated into the following groups: normal, model, pirfenidone (50 mg/kg), CISCFE-L, −M, and -H (240, 360, and 480 mg/kg/d, i.g., respectively, for 4 weeks). Rats were given BLM (5 mg/kg) via intratracheal installation to establish the IPF model. A549 and MRC-5 cells were stimulated by Wnt-1 to establish a cell model and then treated with CISCFE. Haematoxylin-eosin (H&E) and Masson staining were employed to observe lesions in the lung tissues. Quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot (WB) were performed to observe changes in genes and proteins connected with the Wnt/β-catenin pathway. Results CISCFE inhibited the proliferation of MRC-5 cells (IC50: 2.723 ± 0.488 μg/mL) and A549 cells (IC50: 2.235 ± 0.229 μg/mL). In rats, A549 cells, and MRC-5 cells, BLM and Wnt-1 obviously induced the protein expression of α-smooth muscle actin (α-SMA), vimentin, type I collagen (collagen-I), and Nu-β-catenin. The mRNA levels of matrix metalloproteinase-3 (MMP-3) and − 9 (MMP-9), two enzymes that degrade and reshape the extracellular matrix (ECM) were also increased while those of tissue inhibitor of metalloproteinase 1 (TIMP-1) were decreased. However, CISCFE reversed the effects of BLM and Wnt-1 on the expression pattern of these proteins and genes. Conclusion These findings showed that CISCFE could inhibit IPF development by activating the Wnt/β-catenin pathway and may serve as a treatment for IPF after further investigation.

We study the Bc+ production in Pb-Pb collisions at sNN=5.02 TeV. In the quark-gluon plasma (QGP) produced in heavy-ion collisions, heavy quarks make random motions with the energy loss. We employ the Langevin equations to study the non-equilibrium distributions of heavy quarks and the Instantaneous Coalescence Model (ICM) to study the hadronization process. Due to abundant charm and bottom quarks in the QGP, their coalescence probability is significantly enhanced compared with the situations in proton-proton collisions. We find that the final production of Bc+ is increased by the coalescence process, which makes the nuclear modification factor (RAA) of Bc+ larger than unit. Our model explains the experimental data well at semi-central and central collisions. The observation of RAA(Bc+)>1 is regarded as an evident and strong signal of the existence of the deconfined medium generated in heavy-ion collisions.

Acute lung injury (ALI), hallmarked with alveolar epithelial barrier impairment and pulmonary edema induced by acute inflammation, presents a severe health burden to the public, due to the limited available interventions. Oxyberberine (OBB), having improved anti-inflammatory activity and safety, is a representative component with various activities derived from berberine, whereas its role against ALI with alveolar epithelial barrier injury remains uncertain. To investigate the influence and underlying mechanisms of OBB on ALI, we induced acute inflammation in mice and A549 cells by using lipopolysaccharide (LPS). Changes in alveolar permeability were assessed by analyzing lung histopathology, measuring the dry/wet weight ratio of the lungs, and altering proinflammatory cytokines and neutrophils levels in the bronchoalveolar lavage fluid (BALF). Parameters of pulmonary permeability were assessed through ELISA, western blotting, quantitative real-time PCR, and immunofluorescence analysis. U46619, the agonist of RhoA/ROCK, was employed to further investigate the mechanism of OBB on ALI. Unexpectedly, we found OBB mitigated lung impairment, pulmonary edema, inflammatory reactions in BALF and lung tissue, reduction in ZO-1, and addition of connexin-43. Besides, OBB markedly reduced the expression of RhoA in association with its downstream factors, which are linked to the intercellular junctions and permeability both in vivo and in vitro. Nevertheless, U46619 abolished the benefits obtained from OBB in A549 cells. In conclusion, these outcomes indicated that OBB exerted RhoA/ROCK inhibitor-like effect to moderate alveolar epithelial barrier impairment and permeability, ultimately preventing ALI progression.

BackgroundKnee osteoarthritis (KOA) is the leading cause of pain and stiffness, affecting older adults’ physical function and quality of life. As a form of mind-body exercise, Tai Chi has been recommended as an exercise prescription for KOA patients. This study examined the effects and continuation of modified Tai Chi exercises on physical function and quality of life in elderly women with KOA.MethodsWe conducted a single-blind, randomized controlled trial (RCT) on 40 older women with KOA. The participants were randomized to a 12 weeks Tai Chi or control group. The Tai Chi group attended a kind of modified Tai Chi training sessions three times per week; the control group attended wellness education sessions once a week. The primary outcome was the Western Ontario and McMaster University Osteoarthritis Index (WOMAC). Secondary outcomes were the Berg Balance Scale (BBS), Timed Up and Go (TUG), Short-Form 36 (SF-36), Pittsburgh Sleep Quality of Index (PSQI), Self-rating Anxiety Scale (SAS), and Self-rating Depression Scale (SDS).ResultsAfter the 12-weeks the Tai Chi group showed significan improvement in the WOMAC pain (mean difference, −5.09 points, p = 0.001), WOMAC stiffness (mean difference, −3.60 points, p = 0.002), WOMAC physical function (mean difference, −11.21 points, p = 0.001) compared to the control group. In addition, the Tai Chi group had also significant improvement in the BBS (mean difference, 1.70 points, p = 0.008), TUG (mean difference, −0.52s, p = 0.001), SF-36PCS (mean difference, 7.60 points, p = 0.001), MCS (mean difference, 7.30 points, p = 0.001), PSQI (mean difference, −3.71 points, p = 0.001), SDS (mean difference, −5.37 points, p = 0.025) and SAS (mean difference, −5.06 points, p = 0.002).ConclusionThe modified Tai Chi exercises are an effective treatment for improved physical function and quality of life in elderly women with KOA.Clinical Trial RegistrationThe trial was registered in Chinese Clinical Trial Registry (ChiCTR2000040721), http://www.chictr.org.cn/edit.aspx?pid=65419&htm=4.

Using an anti-icing coating to prevent ice accretion on the drill surface is a feasible solution to address the drilling difficulties in warm ice. In this study, four types of commercially available hydrophobic coating materials were tested to evaluate their water repellency and anti-icing properties, namely, a mixture of silica and fluorocarbon resin with polytrifluoroethylene, modified Teflon, silica-based emulsion and an acrylic-based copolymer. Their water contact angles are ~107°, 101°, 114° and 95°, respectively. All these hydrophobic coatings can significantly reduce the strength of the ice adhesion within a temperature range of −10 to −30°C on a planar or curved surface. The coating of an acrylic-based copolymer, in particular, can reduce the average tensile strength and the shear strength of the ice adhesion by 87.08 and 97.11% on planar surfaces at −30°C, and by 98.06 and 96.15% on a curved surface, respectively. The main challenge in the practical application of these coatings is their durability. An acrylic-based copolymer coating will lose its water repellency performance after 140 cycles of abrasion. The shear strength of ice adhered on curved surfaces coated with this material will approach that achieved on uncoated surfaces after 11 cycles of icing and de-icing tests.

A new type of ice core drill bit, designed with a vane swirler, was developed for ice core drilling with air reverse circulation. An orthogonal experimental design method was employed to investigate the effects of the swirler structure parameters on the reverse circulation performance of the drill bit including helical angle, number of blades, blade length and blade central angle, etc. The entrainment ratio was used to evaluate the reverse circulation effectiveness of the drill bit. The results show that the helical angle is the dominant factor regardless of whether or not the flushing nozzles are part of the design of the drill bit. The number of blades is the least important factor for the drill bit designed with the flushing nozzles (referred to as drill bit I), while the outlet area of the swirling slot is the least influential factor for the drill bit without flushing nozzles (referred to as drill bit П). In addition, the appearance of the ice core has a certain effect on the air reverse circulation for both drill bits. Within the ranges of this study, the optimal structure of the drill bit was determined based on the range analysis of the orthogonal design.

Abstract Defoaming is a key technology for increasing the efficiency of foam drilling in petroleum engineering. To enhance the performance of a mechanical foam breaker in foam drilling, a novel aerodynamic foam breaker with two annular slits was investigated in this study. The computational fluid dynamics code of ANSYS Fluent was used to simulate the velocity and pressure distribution inside the foam breaker, and the optimum distance between the two annular slits was determined based on the simulation methods. Meanwhile, a series of experiments were conducted to test the actual performance of the foam breaker. The results demonstrate that various factors may affect the efficiency of the foam breaker, including the foam gas‐liquid ratio, basic liquid viscosity, and air supply method. A higher gas‐liquid ratio of the foam and air supply pressure result in a superior foam breaker performance. The viscosity of the foam liquid phase exhibits exactly the opposite behavior, meaning that the foam breaker more effectively destroys foam from a lower‐viscosity liquid. This study verifies the practicability of this novel aerodynamic foam breaker and discusses the effects of different parameters on the defoaming percentage, and this study can act as a reference and guidance for subsequent defoaming research.

“Yinghong 9” is a widely cultivated large-leaf variety in South China, and the black tea made from it has a high aroma and strong sweet flavor. “Huangyu” is a light-sensitive tea variety with yellow leaves. It was cultivated from the bud-mutation of “Yinghong 9” and has a very low level of chlorophyll during young shoot development. Due to chlorophyll being involved in carbon fixation and assimilation, the changes in photosynthesis might potentially affect the accumulation of flavor metabolites, as well as the quality of “Huangyu” tea. Although “Huangyu” has a golden yellow color and high amino acid content, the mechanism underlying the formation of leaf color and drinking value remains unclear. The widely targeted metabolomics and GC-MS analysis were performed to reveal the differences of key metabolites in fresh and fermented leaves between “Yinghong 9” and “Huangyu.” The results showed that tea polyphenols, total chlorophyll, and carotenoids were more abundant in “Yinghong 9.” Targeted metabolomics analysis indicated that kaempferol-3-glycoside was more abundant in “Yinghong 9,” while “Huangyu” had a higher ratio of kaempferol-3-glucoside to kaempferol-3-galactoside. Compared with “Yinghong 9” fresh leaves, the contents of zeaxanthin and zeaxanthin palmitate were significantly higher in “Huangyu.” The contents of α-farnesene, β-cyclocitral, nerolidol, and trans-geranylacetone, which were from carotenoid degradation and involved in flowery-fruity-like flavor in “Huangyu” fermented leaves, were higher than those of “Yinghong 9.” Our results indicated that “Huangyu” was suitable for manufacturing non-fermented tea because of its yellow leaf and flowery-fruity-like compounds from carotenoid degradation.

Leaf color is one of the key factors involved in determining the processing suitability of tea. It relates to differential accumulation of flavor compounds due to the different metabolic mechanisms. In recent years, photosensitive etiolation or albefaction is an interesting direction in tea research field. However, the molecular mechanism of color formation remains unclear since albino or etiolated mutants have different genetic backgrounds. In this study, wide-target metabolomic and transcriptomic analyses were used to reveal the biological mechanism of leaf etiolation for ‘Huangyu’, a bud mutant of ‘Yinghong 9’. The results indicated that the reduction in the content of chlorophyll and the ratio of chlorophyll to carotenoids might be the biochemical reasons for the etiolation of ‘Huangyu’ tea leaves, while the content of zeaxanthin was significantly higher. The differentially expressed genes (DEGs) involved in chlorophyll and chloroplast biogenesis were the biomolecular reasons for the formation of green or yellow color in tea leaves. In addition, our results also revealed that the changes of DEGs involved in light-induced proteins and circadian rhythm promoted the adaptation of etiolated tea leaves to light stress. Variant colors of tea leaves indicated different directions in metabolic flux and accumulation of flavor compounds.

We review recent studies about anisotropic flows (v1,v2,v3) of charmonium in the quark-gluon plasma produced in relativistic heavy-ion collisions. Collective flows of the bulk medium are developed due to the anisotropic pressure gradient of the medium. Strongly coupled with the bulk medium, charm quarks carry collective flows from the expanding medium, which will be inherited by the regenerated charmonium via the coalescence process. In event-by-event collisions where nucleon positions fluctuate from the smooth distribution, there is triangularity in the medium initial energy density. Triangular flows of the bulk medium and heavy flavor particles can be developed due to the initial fluctuations. In the longitudinal direction, the rapidity-odd distribution of the initial energy density is induced by the rotation of the medium in non-central heavy-ion collisions. Charmonium suffers biased dissociation along positive and negative x-directions in forward (backward) rapidity. The directed flow of charmonium becomes non-zero. The directed, elliptic and triangular flows (v1,v2,v3) of charmonium come from the anisotropic initial distributions of the medium energy density in the transverse and longitudinal directions.

Mitochondria are the main sites for oxidative phosphorylation and synthesis of adenosine triphosphate in cells, and are known as cellular power factories. The phrase “secondary mitochondrial diseases” essentially refers to any abnormal mitochondrial function other than primary mitochondrial diseases, i.e., the process caused by the genes encoding the electron transport chain (ETC) proteins directly or impacting the production of the machinery needed for ETC. Mitochondrial diseases can cause adenosine triphosphate (ATP) synthesis disorder, an increase in oxygen free radicals, and intracellular redox imbalance. It can also induce apoptosis and, eventually, multi-system damage, which leads to neurodegenerative disease. The catechin compounds rich in tea have attracted much attention due to their effective antioxidant activity. Catechins, especially acetylated catechins such as epicatechin gallate (ECG) and epigallocatechin gallate (EGCG), are able to protect mitochondria from reactive oxygen species. This review focuses on the role of catechins in regulating cell homeostasis, in which catechins act as a free radical scavenger and metal ion chelator, their protective mechanism on mitochondria, and the protective effect of catechins on mitochondrial deoxyribonucleic acid (DNA). This review highlights catechins and their effects on mitochondrial functional metabolic networks: regulating mitochondrial function and biogenesis, improving insulin resistance, regulating intracellular calcium homeostasis, and regulating epigenetic processes. Finally, the indirect beneficial effects of catechins on mitochondrial diseases are also illustrated by the warburg and the apoptosis effect. Some possible mechanisms are shown graphically. In addition, the bioavailability of catechins and peracetylated-catechins, free radical scavenging activity, mitochondrial activation ability of the high-molecular-weight polyphenol, and the mitochondrial activation factor were also discussed.

Tissue engineering is a promising approach to restore or replace a damaged temporomandibular joint (TMJ) disc. However, constructing a scaffold that can mimic biomechanical and biological properties of the natural TMJ disc remains a challenge. In this study, three-dimensional (3D) printing technology was used to fabricate polycaprolactone (PCL)/polyurethane (PU) scaffolds and PU scaffolds to imitate the region-specific biomechanical properties of the TMJ disc. The scaffolds were coated with polydopamine (PDA) and combined with a decellularized matrix (dECM). Then, rat costal chondrocytes and mouse L929 fibroblasts, respectively, were suspended on the composite scaffolds and the biological functions of the cells were studied. The properties of the scaffolds were characterized by scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), contact angle analysis, and biomechanical testing. To verify the biocompatibility of the scaffolds, the viability, proliferation, and extracellular matrix (ECM) production of the cells seeded on the scaffolds were assessed by LIVE/DEAD staining, Cell Counting Kit-8 assay, biochemical content analysis, immunofluorescence staining, and qRT-PCR. The functionalized hybrid scaffolds were then implanted into the subcutaneous space of nude mice for 6 weeks, and the regenerated tissue was evaluated by histological staining. The biomechanical properties of PCL/PU and PU scaffolds were comparable to that of the central and peripheral zones, respectively, of a native human TMJ disc. The PDA-coated scaffolds displayed superior biomechanical, structural, and functional properties, creating a favorable microenvironment for cell survival, proliferation, ECM production, and tissue regeneration. In conclusion, 3D-printed polymer scaffolds coated with PDA and combined with dECM hydrogel were found to be a promising substitute for TMJ disc tissue engineering.

Brucea javanica oil (BJO), one of the main products of Brucea javanica, has been widely used in treating different kinds of malignant tumors. Quassinoids are the major category of anticancer phytochemicals of B. javanica. However, current researches on the anti-cancer effect of BJO mainly focused on oleic acid and linoleic acid, the common major components of dietary edible oils, essential and characteristic components of B. javanica like quassinoids potentially involved remained unexplored. In the current investigation, we developed an efficient HPLC method to detect brusatol, a characteristic quassinoid, and comparatively scrutinized the anti-hepatocellular carcinoma (anti-HCC) effect of BJO, brusatol-free BJO (BF-BJO), and brusatol-enriched BJO (BE-BJO) against hepatoma 22 (H22) in mice. High-performance liquid chromatography (HPLC) was utilized to identify the components in BJO. BE-BJO was extracted with 95 % ethanol. The anti-tumor effect of BJO, BF-BJO and BE-BJO was comparatively investigated, and the potential underlying mechanism was explored in H22 ascites tumor-bearing mice. The results indicated that BJO and BE-BJO significantly prolonged the survival time of H22 ascites tumor-bearing mice, while BF-BJO exhibited no obvious effect. BJO and BE-BJO exhibited pronounced anti-HCC activity by suppressing the growth of implanted hepatoma H22 in mice, including ascending weight, abdominal circumference, ascites volume and cancer cell viability, with a relatively wide margin of safety. BJO and BE-BJO significantly induced H22 cell apoptosis by upregulating the miRNA-29b gene level and p53 expression. Furthermore, BJO and BE-BJO treatment substantially downregulated Bcl-2 and mitochondrial Cytochrome C protein expression, and upregulated expression levels of Bax, Bad, cytosol Cytochrome C, caspase-3 (cleaved), caspase‑9 (cleaved), PARP and PARP (cleaved) to induce H22 cells apoptosis. Brusatol was detected in BJO and found to be one of its major active anti-HCC components, rather than fatty acids including oleic acid and linoleic acid. The anti-HCC effect of BJO and BE-BJO was intimately associated with the activation of miRNA-29b, p53-associated apoptosis and mitochondrial-related pathways. Our study gained novel insight into the material basis of BJO in the treatment of HCC, and laid a foundation for a novel specific standard for the quality evaluation of BJO and its commercial products in terms of its anti-cancer application.

A reverse circulation Down-The-Hole (DTH) hammer drill bit in Casing-while-Drilling (CwD) processes is designed and applied to drilling under complicated formation. The drill bit is a special retractable drill bit with an exclusive reverse circulation gas channel. Using numerical simulations and experiments, the influence of the gas channel structure parameters of the drill bit, including the inner jet nozzles, flushing nozzles, suction channel, and other parameters, on its reverse circulation performance is analyzed, and the optimal gas channel structure parameters of the drill bit are determined to improve the reverse circulation effect. The results show that the flushing nozzles and inner jet nozzles have an important influence on entrainment performance. The entrainment rate η decreases as the flushing nozzle diameter increases and decreases as the inner jet nozzle diameter increases. An increase in the suction channel diameter can improve the reverse circulation effect of the drill bit. The spiral slot drill bit is more conducive to air being sucked into the central channel in the form of spiral flow, so it can improve the entrainment performance. The entrainment rate η can reach 23.4% with the optimum structured drill bit.

Patchouli alcohol (PA), a tricyclic sesquiterpene, is the major chemical component of patchouli oil. This study investigated the antidepressant-like effect and mechanism of PA in chronic unpredictable mild stress (CUMS). Our results showed that PA markedly attenuated CUMS-induced depressant-like behaviors, including an effective increase of sucrose preference and spontaneous exploratory capacity, as well as reduction of immobility time. In addition, PA markedly attenuated CUMS-induced mTOR, p70S6K, and 4E-BP-1 phosphorylation reduction in the hippocampus. Furthermore, PA reversed CUMS-induced increases in LC3-II and p62 levels and CUMS-induced decrease in PSD-95 and SYN-I levels. These results indicated that the antidepressant-like effect of PA was correlated with the activation of the mTOR signaling pathway. Moreover, behavioral experimental results showed that the antidepressant-like effect of PA was blocked by rapamycin (autophagy inducer and mTOR inhibitor) and chloroquine (autophagic flux inhibitor). These results suggest that PA exerted antidepressant-like effect in CUMS rats through inhibiting autophagy, repairing synapse, and restoring autophagic flux in the hippocampus by activating the mTOR signaling pathway. The results render PA a promising antidepressant agent worthy of further development into a pharmaceutical drug for the treatment of depression.

Charmonium directed flows are studied based on transport model coupled with the realistic three dimensional expansions of the bulk medium. The non-central symmetric nucleus-nucleus collisions can generate the rotating quark-gluon plasma (QGP) with symmetry-breaking longitudinal distributions. In sNN=200 GeV Au+Au semi-central collisions, charmonium are primordially produced in the initial hard process, they are mainly dissociated by the initial tilted source with high temperatures and then move out of the bulk medium to keep the early information of the medium. The momentum distribution of primordially produced charmonia is less affected by the hydrodynamic expansions of QGP where its tilted shape is being diluted. This biased dissociation can generate directed flows of J/ψ and ψ(2S) which are much larger than the values of light charged hadrons and open heavy flavor. Charmonium directed flows can help to quantify the rapidity-odd distributions of QGP initial energy densities in nucleus-nucleus collisions.

Abstract The on-shell diagram is a very important tool in studying scattering amplitudes. In this paper we discuss the on-shell diagrams without external BCFW bridges. We introduce an extra step of adding an auxiliary external momentum line. Then we can decompose the on-shell diagrams by removing external BCFW bridges to a planar diagram whose top-form is well known now. The top-form of the on-shell diagram with the auxiliary line can be obtained by adding the BCFW bridges in an inverse order as discussed in our former paper (Chen et al. in Eur Phys J C 77(2):80 2017). To get the top-form of the original diagram, the soft limit of the auxiliary line is needed. We obtain the evolution rule for the Grassmannian integral and the geometry constraint in the soft limit. This completes the top-form description of leading singularities in nonplanar scattering amplitudes of $$\mathcal {N}=4$$ N=4 Super Yang–Mills (SYM), which is valid for arbitrary higher-loops and beyond the Maximally-Helicity-Violation (MHV) amplitudes.

We study the charmonium coherent photoproduction and hadroproduction consistently with modifications from both cold and hot nuclear matters. The strong electromagnetic fields from fast moving nucleus interact with the other target nucleus, producing abundant charmonium in the extremely low transverse momentum region pT

We study the J/ψ production based on coalescence model at sNN=2.76 and 5.02 TeV Pb–Pb collisions. With the colliding energy increasing from 2.76 TeV to 5.02 TeV, the number of charm pairs is enhanced by more than 50%. However, the ratio of J/ψ inclusive nuclear modification factors RAA5.02 TeV/RAA2.76 TeV is only about 1.1∼1.2. We find that the regeneration of J/ψ is proportional to the densities of charm and anti-charm quarks, instead of their total numbers. The charm quark density is diluted by the strong expansion of quark gluon plasma, which suppresses the combination probability of heavy quarks and J/ψ regeneration. This effect is more important in higher colliding energies where QGP expansion is strong. We also propose the ratio NJ/ψ/(Nc)2 as a measurement of c and c¯ coalescence probability, which is only affected by the heavy quark diffusions in QGP, and does not depend on the inputs such as cold nuclear matter effects and cross sections of charm quark production. Further more, we give the predictions at the energy of Future Circular Collider (sNN=39 TeV).

We employ the Langevin equation and Wigner function to describe the bottom quark dynamical evolutions and their formation into a bound state in the expanding Quark Gluon Plasma (QGP). The additional suppressions from parton inelastic scatterings are supplemented in the regenerated bottomonium. Hot medium modifications on ϒ(1S) properties are studied consistently by taking the bottomonium potential to be the color-screened potential from Lattice results, which affects both ϒ(1S) regeneration and dissociation rates. Finally, we calculated the ϒ(1S) nuclear modification factor RAArege from bottom quark combination with different diffusion coefficients in Langevin equation, representing different thermalization of bottom quarks. In the central Pb–Pb collisions (b=0) at sNN=5.02 TeV, we find a non-negligible ϒ(1S) regeneration, and it is small in the minimum bias centrality. The connections between bottomonium regeneration and bottom quark energy loss in the heavy ion collisions are also discussed.

We study cold and hot nuclear matter effects on charmonium production in p+Pb collisions at sNN=5.02 TeV in a transport approach. At the forward rapidity, the cold medium effect on all the cc¯ states and the hot medium effect on the excited cc¯ states only can explain well the J/ψ and ψ′ yield and transverse momentum distribution measured by the ALICE collaboration, and we predict a significantly larger ψ′ pT broadening in comparison with J/ψ. However, we can not reproduce the J/ψ and ψ′ data at the backward rapidity with reasonable cold and hot medium effects.

A new type of drill bit designed with an annular slit was developed to enhance the reverse circulation effect of the down-the-hole hammer drilling technology. A computational fluid dynamics code, Fluent, was used to simulate the flow phenomena inside the drill bit. The simulation results show that the air flowing through the annular slit moves upward along the wall of the central passage of the annular drill bit and that there is no interference phenomenon similar to the normal drill bit, which is beneficial for the formation of reverse circulation. Meanwhile, the new drill bit with the annular slit was produced and tested in the laboratory. The results show that for the annular drill bit with the flushing nozzles closed, the mass flow rate of the sucked air is approximately 63.78 g/s, which is 1.76 times that of the normal drill bit, while it is about 2.46 times if the flushing nozzles are opened. In addition, many factors can affect the reverse circulation effect of the annular drill bit, including the slit width, the distance between the annular slit and the working surface of the drill bit, and the flow direction of the gas ejected from the annular slit.