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Background: Kinesin family member 22 (KIF22) is known as a regulator of cell mitosis and cellular vesicle transport. The alterations of KIF22 are associated with a series of tumors; however, its po...

Ras-Related Protein Rab-38 (RAB38), which belongs to the RAB family, is involved in the biogenesis of lysosome-related organelles and defense against certain microbial infections. However, the clinical significance and potential function of RAB38 in pancreatic adenocarcinoma remain unclear. In the present study, an immunohistochemical assay was performed to analyze the expression of RAB38 in pancreatic adenocarcinoma tumor specimens from 82 patients, and the clinicopathological characteristics and survival rate of these patients were further examined. To validate the role of RAB38 in tumors, the effect of RAB38 on tumor cell proliferation, migration and invasion was assessed by establishing RAB38 knockdown cell lines. Reverse transcription-quantitative polymerase chain reaction and western blotting were used to examine the expression levels of proteins associated with the cancer cell behavior. In addition, the inhibitory effect of RAB38 silencing on pancreatic cancer was examined in mice. The immunohistochemistry results revealed that RAB38 was upregulated and positively correlated with the grade of progression in pancreatic adenocarcinoma patients. Further investigation indicated that RAB38 downregulation significantly suppressed the proliferation, migration and invasive capacity of pancreatic cancer cells, as well as decreased the expression levels of Ki67, proliferating cell nuclear antigen, and matrix metalloproteinases 2 and 9. RAB38 silencing also inhibited the development of pancreatic cancer in vivo. Taken together, a high level of RAB38 was significantly associated with the malignant phenotypes of pancreatic cancer, suggesting that RAB38 may serve as a novel biomarker and a potential therapeutic target for pancreatic cancer.

In order to explore the pressure relief and structure stability mechanism of lateral cantilever structure in the stope under the direct coverage of thick hard roof and its impact on the gob-side entry retaining, a lateral cantilever fractured structural mechanical model was established on the basis of clarification for the stress environment of gob-side entry retaining, and the equation of roof given deformation and the balance judgment for fracture block were obtained. The optimal cantilever length was proposed based on the comparison of roof structural characteristics and the stress, deformation law of surrounding rocks under six different cantilever lengths by numerical simulation method. Double stress peaks exist on the sides of gob-side entry retaining and the entry located in the low stress area. The pressure of gob-side entry retaining can be relieved by reducing the cantilever length. However, due to the impact of arch structure of rock beam, unduly short cantilever would result in insufficient pressure relief and unduly long cantilever would bring larger roof stress which results in intense deformation. Therefore, there is optimal cantilever length, which was 7-8 m in this project that enables to achieve the minimum deformation and the most stabilized rock structure of entry retaining. An engineering case of gob-side entry retaining with the direct coverage of 10 m thick hard limestone roof was put forward, and the measured data verified the reasonability of conclusion.

Through analyzing Dabaoliang geological characteristics, this paper discussed the factors of the mechanism of landslide in Dabaoliang area and simulated its evolutionary process. The paper could get some conclusions, and the factors of landslide are as follow: the stability of the slope which mainly composed by clastic rock not only depended on the performance of the mudstone layer but also the skid resistance of the sliding surface and the shape of slope. Under the influence of regional structure, the joint fissures which developed along the boundary of the landslide will connect the crack and benefit the permeation of the surface water. The heavy rain is one of the main factors caused the slope unstable and sliding. Provide effective prevention and control for prevention and control of projects.

On the bases of theoretic analysis, numerical simulation, and project practice, stress distribution of surrounding rock and movement characteristics of roof strata in T-junction area of gob-side entry were analyzed. Besides, control technology in T-junction area was proposed after indicating deformation characteristics of different stages in gob-side entry. Results show that there is an inclined block in T-junction area after fracture of main roof and the period when the block is tending to stability is a critical stage of pressure appearance for gob-side entry. Further, three stress zones: in-situ stress zone, abutment pressure zone and low stress zone appear in both strike and dip of mining panel and enhanced support of gob-side entry before excavation should keep away from the abutment pressure zone, which also indicates that deep bolting support can exert the bearing capacity of deep rock mass. Additionally, the whole procedure of surrounding rock deformation can be divided into four stages and deformation in gob-side entry is larger than that of entry excavation, which means that efficient support design should be taken into account. Ultimately, district control technologies for surrounding rock of gob-side entry in back zone, filling zone, and front zone are proposed and it showed a good adaption in project practice of coalmine.

Disc Sickle; Acoustic Emission evaluation; pitting corrosion damage Abstract. By the pitting corrosion simulation test in the laboratory, the law that the Disc Sickle corrosion degree and AE signal vary with corrosion time has been studied, and relationship curve between corrosion damage and AE parameter has been obtained. This explains that AE technology can be used for detecting forepart pitting corrosion, researching the law of pitting corrosion development, and evaluating corrosion damage.

Effects of Al content and heat treatment on the structure, hardness and electrochemical properties of FeCoNiCrCu0.5Alx high-entropy alloys were investigated. The phase structure of as-cast alloys evolves from FCC phase to BCC phase with the increase of Al content. The stable phase of FeCoNiCrCu0.5Alx high-entropy alloys will transform from FCC phase to FCC+BCC duplex phases when x value increases from 0.5 to 1.5. The hardness of BCC phase is higher than that of FCC phase, and the corrosion resistance of BCC phase is better than FCC phase in chlorine ion and acid medium. High hardness and good corrosion resistance can be obtained in as-cast FeCoNiCrCu0.5Al1.0 alloy.

By the method of computational fluid dynamics (CFD), this paper calculates the aerodynamic parameters of one complex high-speed train model which adopts different support forms when the reference velocity is 70m/s under different operating conditions. It also analyses the support interference mechanism from the point of flow field structure. The results show that the distributed cylinder support form causes least interference on the model, while the single big cylinder support forms change the flow field structures much which leads to much change of the aerodynamic parameters of the model. The distributed cylinder support form can be applied as a good support form for the high-speed train wind tunnel tests.

A three factors quadratic regression rotation combination design was adopted to optimize the technical conditions of microwave low temperature carbon dioxide flash puffing (MLTCDFP) process for dwarf banana in the single factor’s foundation. This paper analyzed the effect of puffing temperature, puffing pressure, vacuum drying temperature and the interaction of the three factors on color, crispness, and the effect of hardness and water content. Based on the experimental data, the quadratic regression model of four indexes was deduced, then variables were analyzed with response surface methodology (RSM). The weights of four evaluation indexes was determined by factor analysis. The regression square was obtained by the comprehensive score as follow: Y=0.955+0.109X 1 +0.16X 3 −0.09X 1 2 +0.015X 2 2 −0.15X 3 2 −0.018X 1 X 2 −0.056X 1 X 3 +0.016X 2 X 3 (R 2 =0.837).Through factor analysis, the range of the optimal technical conditions of MLTCDFP for dwarf banana obtained. The result indicated the four indexes were affected significantly by the puffing temperature and vacuum drying temperature, and the interactions of the three factors did not affect the product obviously. The optimal technical parameters were as follows: puffing temperature 91~95°C, puffing pressure 0.11~0.19MPa, vacuum temperature 81~85°C.

Capillary tube, a efficient and energy conservation heat exchanger, has been widely used abroad. In china, the research of air-condition terminal based on capillary tube is focus on the planar radiation at the moment. But kinds of problems such as moisture condensation happen because of the different climate of China. So we put forward developing a kind of breathing air-condition cabinet combined with heat exchange and dehumidification, and do some calculation about major parameter. We will do in-depth study on cabinet's flow field so that it can be high-efficiency.

From 2001 to 2005, a new railroad linking Beijing with Lhasa was built by more than 100,000 workers, of whom 80% traveled from their lowland habitat to altitudes up to 5000 m to work on the railroad. We report on the medical conditions of 14,050 of these altitude workers, specifically with regard to preexisting illness. All subjects were seen at low and high altitude. Average age was 29.5 +/- 7.4 (SD) yr, range 20 to 62 yr; 98.8% of the subjects were men and 1.2% were women. Overall incidence of AMS upon first-time exposure was 51%, that of HACE 0.28%, and that of HAPE 0.49%. About 1% of the subjects were hypertensive before altitude exposure. Those with blood pressure >or=160/95 were excluded from employment at altitude. Altitude exposure led to a greater increase of blood pressure in hypertensives compared to normotensives. On prealtitude screening prevalence of cardiac arrhythmias was 0.33%. Since the majority of these were rather benign and occurring in young and otherwise healthy subjects, we allowed altitude employment. Follow-up at altitude was uneventful. Subjects with coronary heart disease and diabetes were excluded from altitude employment. Obesity was a risk factor for acute mountain sickness and for reduced work performance at altitude. Overweight subjects lost more weight during their altitude stay than subjects with normal weight. Altitude exposure was a risk factor for upper gastrointestinal tract bleeding, especially in combination with alcohol, aspirin, and dexamethasone intake. Asthmatic subjects generally did better at altitude compared to low altitude, with the exception of one subject who experienced an asthma episode from pollen exposure. In conclusion, careful evaluation of preexisting chronic illness and risk factors allowed prevention of altitude deterioration of a preexisting health condition, all the while allowing subjects with some specific conditions to work and live at altitude without problems.

A new chiral polymer–metal complex, silica-supported alginic acid-L-glutamic acid–Pt complex (SiO2-AA-L-Glu-Pt), has been prepared by a very simple method and has been found to be able to catalyze the asymmetric hydrogenation of 4-methyl-2-pentanone to give (R)-(−)-4-methyl-2-pentanol in 90.8% optical yield at 30 °C and under 1 atm H2. The product and optical yields were greatly affected by the Pt content in the complex, the solvent, the 4-methyl-2-pentanone/Pt molar ratio and the reaction temperature. The catalyst could be reused several times without any great change in its optical catalytic activity. Copyright © 2003 John Wiley & Sons, Ltd.

The diagnostic features are the basis to detect and characterize the oil film on water through optical remote sensing. This work shows the results of lab spectral measurements of light diesel oil with thickness ranged 1.0 - 127 microm. A wavelet transform were performed to the reflectance, and the singularity (388-393 nm) was explored as the indicators of oil film thickness. The results indicate that the reflectance of light diesel oil film is higher than that of water in the range from 350-2 500 nm. There is a reflectance peak near 388 nm when the thickness of oil film is larger than 6 microm, however, no distinguished features could be recognized when oil films were thinner than 6 microm. The wavelet coefficients of the fifth decomposition level by applying Daubechies 4 (db4) mother wavelets proved successful for identifying the singularity of oil film's reflectance spectra and its accurate position. With the thickness lager than 6 microm, the detail coefficients performed an abrupt change within the range of 388-393 nm, and became more violent while oil films' thickness increased. This research demonstrated that oil films on water with different thickness could be distinguished based on wavelet detail coefficients, with important implications for detection of oils on water using UV and short wave optical remote sensing.

To investigate the mechanism through which insulin affect the learning and memory abilities of the Alzheimer's disease-like rats.Okadaic acid (OA) was injected into the CA1 region of the rat hippocampus and the insulin was injected into the lateral cerebral ventricle of the rats. The learning and memory abilities of the rats were assessed through Morriswater maze behavioral test, and the expressions of nicotinic acetylcholine receptors and GFAP were observed by Westem blotting and immunohistochemistry, respectively.Compared with the control rats, the abilities of learning and memory were lowered significantly (P0.01) and the expressions of the nicotinic acetylcholine receptors were decreased and the GFAP positive astrocytes were increased greatly in the model rats (P0.05). In the rats injected with insulin, it was found that their learning and memory abilities were improved significantly (P0.01) and that the expression of the nicotinic acetylcholine receptors were increased and GFAP positive astrocytes were decreased obviously (P0.05), as compared with the model rats.Insulin is able to enhance the learning and memory abilities of the Alzheimer's disease-like rats, possibly by improving the function of the acetylcholine system and decreasing the astrocytes proliferation in the brain.

To investigate the effects of intermittent hypoxic exposure and normoxic convalescence on the parameter of erythrocyte and serum hypoxia inducible factor-1 alpha (HIF-1alpha) and erythropoietin (EPO) levels.Rat models of intermittent hypoxic exposure were established, combined with the clinical research on volunteers experiencing the intermittent plateau work. Blood samples for red blood cell (RBC) counts, hemoglobin (Hb) and hematocrit (HCT) were collected, serum HIF-1alpha and EPO levels were measured using enzyme linked immunosorbent assay.RBC counts, Hb concentration and HCT were significantly higher than the normoxic group (P0.05), after exposure of rats to hypoxia from 7 to 28 days. Compared with the normoxic group, serum HIF-1alpha levels were higher in the group of IH3, 7, 14 days, and EPO had a corresponding increase in the group of IH3, 7 days. Then, a decrease was observed in parameter of erythrocyte and serum HIF-1alpha and EPO levels after 14 days normoxic convalescence treat. In volunteers studies, RBC counts in 8 months group and Hb concentration in 2 years group were significantly higher than the plain group (P0.05). Compared with the plain group, EPO had no significant differences in any of plateau group.Intermittent hypoxic exposure can enhance serum hypoxia inducible factor-1 alpha and erythropointin levels and the generation of red blood cells, which leads to an increase in hemoglobin concentration and hematocrit. The results have changed with the hypoxic exposure period prolonged. Normoxic convalescence after intermittent hypoxic exposure can make the related indexes reduced, and contribute to the organism recovery.

It takes ≈24 h to travel the ≈3000-km-long Qinghai–Tibet railroad of which 85% is situated above 4000 m with a pass at 5072 m. Each year about 2 million passengers are rapidly exposed to high altitude traveling on this train. The aim of this study was to quantify the occurrence of altitude illness on the train. Three subject groups were surveyed: 160 Han lowlanders, 62 Han immigrants living at 2200 to 2500 m, and 25 Tibetans living at 3700 to 4200 m. Passengers reached 4768 m from 2808 m in less than 1.5 h, after which 78% of the passengers reported symptoms, 24% reaching the Lake Louise criterion score for AMS. AMS incidence was 31% in nonacclimatized Han compared to 16% in Han altitude residents and 0% in Tibetans. Women and older subjects had a slightly greater risk for AMS. Most cases of AMS were mild and self-limiting, resolving within days upon arrival in Lhasa. Some cases of more severe AMS necessitated medical attention. To curb the health risk of rapid travel to altitude by train, prospective travelers should be better informed, medical train personnel should be well trained, and staged travel with 1 to 2 days at intermediate altitudes should be suggested to nonacclimatized subjects.

The one-step single-tube betaine-free reverse transcription (RT)-loop-mediated isothermal amplification assay was developed for rapid diagnosis of hepatitis E virus. This assay amplified the target gene in less than 45 min (even as short as 20 min) under isothermal conditions at 63°C, and the sensitivity of this assay was 100-fold greater than that of RT-PCR. This assay demonstrated a detection limit of 0.045 fg (nine copies/reaction).