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5. Structural Characterization and Anti-inflammatory Activities of Anticomplementary Polysaccharides from Rhododendron principis.

Author

Zhao, Zhi-Zhi, Zhang, Jie, Hong, Zhou, Bao, Wei-Lian, Zhou, Li-Shuang, Liu, Yang, Chen, Dao-Feng, and Lu, Yan

Subjects
POLYSACCHARIDES, INTERLEUKINS, LIPOPOLYSACCHARIDES, MEDICINAL plants, COMPLEMENT (Immunology), BRONCHOALVEOLAR lavage, ANIMAL experimentation, MONOSACCHARIDES, ONE-way analysis of variance, ANTIOXIDANTS, LEAVES, METHYLATION, DESCRIPTIVE statistics, RESEARCH funding, PLANT extracts, MOLECULAR structure
Abstract

Rhododendron principis leaves have been used as "Dama", a Traditional Tibetan Medicine for treating inflammatory diseases. R. principis crude polysaccharides with anticomplementary activity demonstrated promising anti-inflammatory effects on acute lung injury induced by lipopolysaccharide. R. principis crude polysaccharides significantly decreased the levels of TNF- α and interleukin-6 in both serum and blood and bronchoalveolar lavage fluid in lipopolysaccharide-induced acute lung injury mice by intragastric administration (100 mg/kg). A heteropolysaccharide, ZNDHP, was obtained from R. principis crude polysaccharides with successive anticomplementary activity-guided separation. ZNDHP was characterized as a branched neutral polysaccharide with a backbone composed of → 2)- β -Glc p -(1→, → 2,6)- α -Glc p -(1→, → 6,3)- β -Gal p -(1→, → 2,6)- α -Gal p -(1→, → 6,2)- β -Glc p -(1→, → 4)- α -Glc p -(1→, → 5)- β -Ara f -(1→, → 3,5)- α -Ara f -(1→, and → 4,6)- β -Man p -(1→, and the backbone structure was further confirmed by partial acid hydrolysis. In addition to anticomplementary and antioxidant activities, ZNDHP exhibited potent anti-inflammatory activity by significantly inhibiting the secretion of nitric oxide, TNF- α , interleukin-6, and interleukin-1 β of lipopolysaccharide-treated RAW 264.7 cells. However, all of these activities decreased greatly after partially hydrolyzing, indicating the importance of the multibranched structure for its bioactivity. Therefore, ZNDHP might be an important component of R. principis for treating inflammation. [ABSTRACT FROM AUTHOR]

Published
2023
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7. Oral Nanoparticles of SNX10-shRNA Plasmids Ameliorate Mouse Colitis

Author

Bao,Wei-Lian, Wu,Qibiao, Hu,Bin, Sun,Dongdong, Zhao,Shengnan, Shen,Xiaoyan, Cheng,Haibo, Shen,Weixing, Bao,Wei-Lian, Wu,Qibiao, Hu,Bin, Sun,Dongdong, Zhao,Shengnan, Shen,Xiaoyan, Cheng,Haibo, and Shen,Weixing

Abstract

Wei-Lian Bao,1,2,* Qibiao Wu,3,* Bin Hu,2 Dongdong Sun,1 Shengnan Zhao,1 Xiaoyan Shen,2 Haibo Cheng,1 Weixing Shen1 1The First Clinical Medical College of Nanjing University of Chinese Medicine, Jiangsu Collaborative Innovation Center of Traditional Chinese Medicine Prevention and Treatment of Tumor, Jiangsu, Nanjing 210023, People’s Republic of China; 2Department of Pharmacology & the Key Laboratory of Smart Drug Delivery, Ministry of Education, School of Pharmacy, Fudan University, Shanghai, People’s Republic of China; 3State Key Laboratory of Quality Research in Chinese Medicines, Faculty of Chinese Medicine, Macau University of Science and Technology, Macau 999078, People’s Republic of China*These authors contributed equally to this workCorrespondence: Weixing Shen; Haibo ChengThe First Clinical Medical College of Nanjing University of Chinese Medicine, Jiangsu Collaborative Innovation Center of Traditional Chinese Medicine Prevention and Treatment of Tumor, 138 Xianling Ave, Nanjing, Jiangsu 210023, People’s Republic of ChinaEmail weixingshen@njucm.edu.cn; haibocheng@njucm.edu.cnBackground: Our previous study found that deletion of Sorting nexin 10 (SNX10) can protect against colonic inflammation and pathological damage induced by dextran sulfate sodium (DSS). This inspired us that modulation of SNX10 expression in colonic epithelial cells might represent a promising therapeutic strategy for inflammatory bowel disease (IBD).Methods: Effective delivery of siRNA/shRNA to silence genes is a highly sought-after means in the treatment of multiple diseases. Here, we encapsulated SNX10-shRNA plasmids (SRP) with polylactide-polyglycolide (PLGA) to make oral nanoparticles (NPs), and then applied them to acute and chronic IBD mice model, respectively. The characteristics of the nanoparticles were assayed and the effects of SRP-NPs on mouse IBD were evaluated.Results: High-efficiency SNX10-shRNA plasmids were succes

Published
2021

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