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303 results on '"Bankstahl, Jens P."'

4. Neuroinflammation imaging markers for epileptogenesis.

Author

Koepp, Matthias J, Årstad, Eric, Bankstahl, Jens P, Dedeurwaerdere, Stefanie, Friedman, Alon, Potschka, Heidrun, Ravizza, Teresa, Theodore, William H, and Baram, Tallie Z

Subjects
Brain, Astrocytes, Animals, Humans, Rats, Brain Injuries, Epilepsy, Seizures, Febrile, Status Epilepticus, Neurogenic Inflammation, Disease Models, Animal, Disease Progression, Vascular Cell Adhesion Molecule-1, Magnetic Resonance Imaging, Image Enhancement, Electroencephalography, Risk Factors, Video Recording, Stroke, Proton Magnetic Resonance Spectroscopy, Biomarkers, Blood-brain barrier, Epileptogenesis, Glia, Magnetic resonance imaging, Nuclear medicine imaging, Prevention, Neurodegenerative, Brain Disorders, Neurosciences, 2.1 Biological and endogenous factors, Neurological, Seizures, Febrile, Disease Models, Animal, Clinical Sciences, Neurology & Neurosurgery
Abstract

Epilepsy can be a devastating disorder. In addition to debilitating seizures, epilepsy can cause cognitive and emotional problems with reduced quality of life. Therefore, the major aim is to prevent the disorder in the first place: identify, detect, and reverse the processes responsible for its onset, and monitor and treat its progression. Epilepsy often occurs following a latent period of months to years (epileptogenesis) as a consequence of a brain insult, such as head trauma, stroke, or status epilepticus. Although this latent period clearly represents a therapeutic window, we are not able to stratify patients at risk for long-term epilepsy, which is prerequisite for preventative clinical trials. Moreover, because of the length of the latent period, an early biomarker for treatment response would be of high value. Finally, mechanistic biomarkers of epileptogenesis may provide more profound insight in the process of disease development.

Published
2017

6. Age-Related Deficits in Binaural Hearing: Contribution of Peripheral and Central Effects.

Author

Tolnai, Sandra, Weiß, Mariella, Beutelmann, Rainer, Bankstahl, Jens P., Bovee, Sonny, Ross, Tobias L., Berding, Georg, and Klump, Georg M.

Subjects
EAR, AUDIOGRAM, MONGOLIAN gerbil, POSITRON emission tomography, SYNAPSES, INNER ear, AUDITORY pathways, AUDITORY perception
Abstract

Pure-tone audiograms often poorly predict elderly humans' ability to communicate in everyday complex acoustic scenes. Binaural processing is crucial for discriminating sound sources in such complex acoustic scenes. The compromised perception of communication signals presented above hearing threshold has been linked to both peripheral and central age-related changes in the auditory system. Investigating young and old Mongolian gerbils of both sexes, an established model for human hearing, we demonstrate age-related supra-threshold deficits in binaural hearing using behavioral, electrophysiological, anatomical, and imaging methods. Binaural processing ability was measured as the binaural masking level difference (BMLD), an established measure in human psychophysics. We tested gerbils behaviorally with "virtual headphones," recorded single-unit responses in the auditory midbrain and evaluated gross midbrain and cortical responses using positron emission tomography (PET) imaging. Furthermore, we obtained additional measures of auditory function based on auditory brainstem responses, auditory-nerve synapse counts, and evidence for central inhibitory processing revealed by PET. BMLD deteriorates already in middle-aged animals having normal audiometric thresholds and is even worse in old animals with hearing loss. The magnitude of auditory brainstem response measures related to auditory-nerve function and binaural processing in the auditory brainstem also deteriorate. Furthermore, central GABAergic inhibition is affected by age. Because the number of synapses in the apical turn of the inner ear was not reduced in middle-aged animals, we conclude that peripheral synaptopathy contributes little to binaural processing deficits. Exploratory analyses suggest increased hearing thresholds, altered binaural processing in the brainstem and changed central GABAergic inhibition as potential contributors. [ABSTRACT FROM AUTHOR]

Published
2024
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11. Imaging of chemokine receptor CXCR4 expression in culprit and nonculprit coronary atherosclerotic plaque using motion-corrected [68Ga]pentixafor PET/CT

Author

Derlin, Thorsten, Sedding, Daniel G., Dutzmann, Jochen, Haghikia, Arash, König, Tobias, Napp, L. Christian, Schütze, Christian, Owsianski-Hille, Nicole, Wester, Hans-Jürgen, Kropf, Saskia, Thackeray, James T., Bankstahl, Jens P., Geworski, Lilli, Ross, Tobias L., Bauersachs, Johann, and Bengel, Frank M.

Published
2018
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15. Preparation and PET/CT imaging of implant directed 68Ga-labeled magnetic nanoporous silica nanoparticles.

Author

Polyak, Andras, Harting, Heidi, Angrisani, Nina, Herrmann, Timo, Ehlert, Nina, Meißner, Jessica, Willmann, Michael, Al-Bazaz, Silav, Ross, Tobias L., Bankstahl, Jens P., and Reifenrath, Janin

Subjects
SILICA nanoparticles, COMPUTED tomography, NANOPARTICLES, BLOOD circulation, HINDLIMB, POLYETHYLENE terephthalate
Abstract

Background: Implant infections caused by biofilm forming bacteria are a major threat in orthopedic surgery. Delivering antibiotics directly to an implant affected by a bacterial biofilm via superparamagnetic nanoporous silica nanoparticles could present a promising approach. Nevertheless, short blood circulation half-life because of rapid interactions of nanoparticles with the host's immune system hinder them from being clinically used. The aim of this study was to determine the temporal in vivo resolution of magnetic nanoporous silica nanoparticle (MNPSNP) distribution and the effect of PEGylation and clodronate application using PET/CT imaging and gamma counting in an implant mouse model. Methods: PEGylated and non-PEGylated MNPSNPs were radiolabeled with gallium-68 (68Ga), implementing the chelator tris(hydroxypyridinone). 36 mice were included in the study, 24 mice received a magnetic implant subcutaneously on the left and a titanium implant on the right hind leg. MNPSNP pharmacokinetics and implant accumulation was analyzed in dependence on PEGylation and additional clodronate application. Subsequently gamma counting was performed for further final analysis. Results: The pharmacokinetics and biodistribution of all radiolabeled nanoparticles could clearly be visualized and followed by dynamic PET/CT imaging. Both variants of 68Ga-labeled MNPSNP accumulated mainly in liver and spleen. PEGylation of the nanoparticles already resulted in lower liver uptakes. Combination with macrophage depletion led to a highly significant effect whereas macrophage depletion alone could not reveal significant differences. Although MNPSNP accumulation around implants was low in comparison to the inner organs in PET/CT imaging, gamma counting displayed a significantly higher %I.D./g for the tissue surrounding the magnetic implants compared to the titanium control. Additional PEGylation and/or macrophage depletion revealed no significant differences regarding nanoparticle accumulation at the implantation site. Conclusion: Tracking of 68Ga-labeled nanoparticles in a mouse model in the first critical hours post-injection by PET/CT imaging provided a better understanding of MNPSNP distribution, elimination and accumulation. Although PEGylation increases circulation time, nanoparticle accumulation at the implantation site was still insufficient for infection treatment and additional efforts are needed to increase local accumulation. [ABSTRACT FROM AUTHOR]

Published
2023
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17. A companion to the preclinical common data elements and case report forms for in vivo rodent neuroimaging: A report of the TASK3‐WG3 Neuroimaging Working Group of the ILAE / AES Joint Translational Task Force

Author

van Vliet, Erwin A., primary, Immonen, Riikka, additional, Prager, Ofer, additional, Friedman, Alon, additional, Bankstahl, Jens P., additional, Wright, David K., additional, O'Brien, Terence J., additional, Potschka, Heidrun, additional, Gröhn, Olli, additional, and Harris, Neil G., additional

Published
2022
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25. Spatio-Temporal Alterations in Synaptic Density During Epileptogenesis in the Rat Brain

Author

Mikkelsen, Jens D., Aripaka, Sanjay S., Bascuñana, Pablo, Bankstahl, Marion, Bankstahl, Jens P., Pazarlar, Burcu A., Mikkelsen, Jens D., Aripaka, Sanjay S., Bascuñana, Pablo, Bankstahl, Marion, Bankstahl, Jens P., and Pazarlar, Burcu A.

Abstract

Synaptic vesicle glycoprotein 2A (SV2A) is a transmembrane protein that binds levetiracetam and is involved in neurotransmission via an unknown mechanism. SV2A-immunoreactivity is reduced in animal models of epilepsy, and in postmortem hippocampi from patients with temporal lobe epilepsy. It is not known if other regions outside the hippocampus are affected in epilepsy, and whether SV2A expression is permanently reduced or regulated over time. In this study, we induced a generalized status epilepticus (SE) by systemic administration of lithium-pilocarpine to adult female rats. The brains from all animals experiencing SE were collected at different time points after the treatment. The radiotracer, [11C]-UCB-J, binds to SV2A with high affinity, and has been used for in vivo imaging as an a-proxy marker for synaptic density. Here we determined the level of tritiated UCB-J binding by semiquantitative autoradiography in the cerebral cortex, hippocampus, thalamus, and hypothalamus, and in cortical subregions. A prominent and highly significant reduction in SV2A binding capacity was observed over the first days after SE in the cerebral cortex and the hippocampus, but not in the thalamus and hypothalamus. The magnitude in reduction was larger and occurred earlier in the hippocampus and the piriform cortex, than in other cortical subregions. Interestingly, in all areas examined, the binding capacity returned to control levels 12 weeks after the SE comparable to the chronic epileptic phase. These data indicate that lithium-pilocarpine-induced epileptogenesis involves both loss and gain of synapses in the in a time-dependent manner.

Published
2022

26. A companion to the preclinical common data elements and case report forms for in vivo rodent neuroimaging: A report of the TASK3-WG3 Neuroimaging Working Group of the ILAE/AES Joint Translational Task Force

Author

van Vliet, Erwin A., Immonen, Riikka, Prager, Ofer, Friedman, Alon, Bankstahl, Jens P., Wright, David K., O'Brien, Terence J., Potschka, Heidrun, Gröhn, Olli, Harris, Neil G., Pathology, APH - Aging & Later Life, APH - Mental Health, and Amsterdam Neuroscience - Cellular & Molecular Mechanisms

Subjects
positron emission tomography, epilepsy, magnetic resonance imaging, rat, magnetic resonance spectroscopy, mouse, single photon emission computed tomography
Abstract

The International League Against Epilepsy/American Epilepsy Society (ILAE/AES) Joint Translational Task Force established the TASK3 working groups to create common data elements (CDEs) for various aspects of preclinical epilepsy research studies, which could help improve the standardization of experimental designs. In this article, we discuss CDEs for neuroimaging data that are collected in rodent models of epilepsy, with a focus on adult rats and mice. We provide detailed CDE tables and case report forms (CRFs), and with this companion manuscript, we discuss the methodologies for several imaging modalities and the parameters that can be collected.

Published
2022

36. Optimization of Artificial Siderophores as Ga-Complexed PET Tracers for In Vivo Imaging of Bacterial Infections

Author

Peukert, Carsten, Langer, Laura N B, Wegener, Sophie M, Tutov, Anna, Bankstahl, Jens P, Karge, Bianka, Bengel, Frank M, Ross, Tobias L, Brönstrup, Mark, and HZI,Helmholtz-Zentrum für Infektionsforschung GmbH, Inhoffenstr. 7,38124 Braunschweig, Germany.

Abstract

The diagnosis of bacterial infections at deep body sites benefits from noninvasive imaging of molecular probes that can be traced by positron emission tomography (PET). We specifically labeled bacteria by targeting their iron transport system with artificial siderophores. The cyclen-based probes contain different binding sites for iron and the PET nuclide gallium-68. A panel of 11 siderophores with different iron coordination numbers and geometries was synthesized in up to 8 steps, and candidates with the best siderophore potential were selected by a growth recovery assay. The probes [68Ga]7 and [68Ga]15 were found to be suitable for PET imaging based on their radiochemical yield, radiochemical purity, and complex stability in vitro and in vivo. Both showed significant uptake in mice infected with Escherichia coli and were able to discern infection from lipopolysaccharide-triggered, sterile inflammation. The study qualifies cyclen-based artificial siderophores as readily accessible scaffolds for the in vivo imaging of bacteria.

Published
2021

38. Synthesis and in vivo evaluation of the putative breast cancer resistance protein inhibitor [ 11C]methyl 4-((4-(2-(6,7-dimethoxy-1,2,3,4-tetrahydroisoquinolin-2-yl)ethyl)phenyl)amino-carbonyl)-2-(quinoline-2-carbonylamino)benzoate

Author

Mairinger, Severin, Langer, Oliver, Kuntner, Claudia, Wanek, Thomas, Bankstahl, Jens P., Bankstahl, Marion, Stanek, Johann, Dörner, Bernd, Bauer, Florian, Baumgartner, Christoph, Löscher, Wolfgang, Erker, Thomas, and Müller, Markus

Published
2010
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40. Simplified 89Zr-Labeling Protocol of Oxine (8-Hydroxyquinoline) Enabling Prolonged Tracking of Liposome-Based Nanomedicines and Cells

Author

Polyak, Andras, primary, Bankstahl, Jens P., additional, Besecke, Karen F. W., additional, Hozsa, Constantin, additional, Triebert, Wiebke, additional, Pannem, Rajeswara Rao, additional, Manstein, Felix, additional, Borcholte, Thomas, additional, Furch, Marcus, additional, Zweigerdt, Robert, additional, Gieseler, Robert K., additional, Bengel, Frank M., additional, and Ross, Tobias L., additional

Published
2021
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48. Proof-of-concept that network pharmacology is effective to modify development of acquired temporal lobe epilepsy

Author

Schidlitzki, Alina, primary, Bascuñana, Pablo, additional, Srivastava, Prashant K., additional, Welzel, Lisa, additional, Twele, Friederike, additional, Töllner, Kathrin, additional, Käufer, Christopher, additional, Gericke, Birthe, additional, Feleke, Rahel, additional, Meier, Martin, additional, Polyak, Andras, additional, Ross, Tobias L., additional, Gerhauser, Ingo, additional, Bankstahl, Jens P., additional, Johnson, Michael R., additional, Bankstahl, Marion, additional, and Löscher, Wolfgang, additional

Published
2020
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