148 results on '"Banie, L"'
Search Results
2. Tunica albuginea allograft: A new model of LaPeyronie's disease with penile curvature and subtunical ossification
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Lue, Tom, Ferretti, L, Fandel, TM, Qiu, X, Zhang, H, Orabi, H, Wu, AK, Banie, L, Wang, G, Lin, G, and Lin, CS
- Abstract
The pathophysiology of LaPeyronie's disease (PD) is considered to be multifactorial, involving genetic predisposition, trauma, inflammation and altered wound healing. However, these factors have not yet been validated using animal models. In this study, we more...
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- 2014
Catalog
3. Tracking intracavernously injected adipose-derived stem cells to bone marrow
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Lin, G, Qiu, X, Fandel, T, Banie, L, Wang, G, Lue, TF, and Lin, C-S
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Medical Biotechnology ,Biomedical and Clinical Sciences ,Regenerative Medicine ,Stem Cell Research ,Stem Cell Research - Nonembryonic - Non-Human ,Transplantation ,Urologic Diseases ,Development of treatments and therapeutic interventions ,5.2 Cellular and gene therapies ,Adipocytes ,Adipose Tissue ,Animals ,Bone Marrow Cells ,Cell Movement ,Erectile Dysfunction ,Flow Cytometry ,Injections ,Male ,Penis ,Rats ,Rats ,Sprague-Dawley ,Stem Cell Transplantation ,adipose-derived stem cells ,bone marrow ,intracavernous injection ,erectile dysfunction ,Clinical Sciences ,Psychology ,Obstetrics & Reproductive Medicine ,Clinical sciences ,Clinical and health psychology - Abstract
The intracavernous (i.c.) injection of stem cells (SCs) has been shown to improve erectile function in various erectile dysfunction (ED) animal models. However, the tissue distribution of the injected cells remains unknown. In this study we tracked i.c.-injected adipose-derived stem cells (ADSCs) in various tissues. Rat paratesticular fat was processed for ADSC isolation and culture. The animals were then subject to cavernous nerve (CN) crush injury or sham operation, followed by i.c. injection of 1 million autologous or allogeneic ADSCs that were labeled with 5-ethynyl-2-deoxyuridine (EdU). Another group of rats received i.c. injection of EdU-labeled allogeneic penile smooth muscle cells (PSMCs). At 2 and 7 days post injection, penises and femoral bone marrow were processed for histological analyses. Whole femoral bone marrows were also analyzed for EdU-positive cells by flow cytometry. The results show that ADSCs exited the penis within days of i.c. injection and migrated preferentially to bone marrow. Allogenicity did not affect the bone marrow appearance of ADSCs at either 2 or 7 days, whereas CN injury reduced the number of ADSCs in bone marrow significantly at 7 but not 2 days. The significance of these results in relation to SC therapy for ED is discussed. more...
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- 2011
4. The impact of co-infections on the haematological profile of East African Short-horn Zebu calves
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VAN WYK, ILANA CONRADIE, GODDARD, AMELIA, DE C. BRONSVOORT, B. MARK, COETZER, JACOBUS A. W., HANDEL, IAN G., HANOTTE, OLIVIER, JENNINGS, AMY, LESOSKY, MAIA, KIARA, HENRY, THUMBI, SAM M., TOYE, PHIL, WOOLHOUSE, MARK W., and PENZHORN, BANIE L. more...
- Published
- 2014
5. Babesiosis of wild carnivores and ungulates
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Penzhorn, Banie L.
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- 2006
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6. Preface to the special issue of the International Congress on Parasites of Wildlife, 15–18 September 2014, Kruger National Park, South Africa
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Banie L. Penzhorn
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Zoology ,QL1-991 - Published
- 2015
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7. STEM CELL THERAPY FOR PEYRONIEʼS DISEASE: MORPHOLOGICAL AND FUNCTIONAL OUTCOMES OF INTRAPLAQUE INJECTION OF ADIPOSE-DERIVED STEM CELLS ON A RAT MODEL OF PEYRONIEʼS DISEASE: PS-04-005
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Ferretti, L., Qiu, X., Fandel, T., Orabi, H., Banie, L., Lin, G., and Lue, T.
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- 2012
8. Pentoxifylline promotes recovery of erectile function after cavernous nerve injury in rats: PS-06-002
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Albersen, M., Fandel, T., Zhang, H., Banie, L., Lin, G., Lin, C.-S., and Lue, T. F.
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- 2010
9. Autochthonous canine babesiosis in The Netherlands
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Matjila, Tshepo P., Nijhof, Ard M., Taoufik, Amar, Houwers, Dirk, Teske, Erik, Penzhorn, Banie L., Lange, Thamar de, and Jongejan, Frans
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- 2005
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10. Feline Babesiosis in South Africa: A Review
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PENZHORN, BANIE L., SCHOEMAN, TANYA, and JACOBSON, LINDA S.
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- 2004
11. History and development of research on wildlife parasites in southern Africa, with emphasis on terrestrial mammals, especially ungulates
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Kerstin Junker, Banie L. Penzhorn, and Ivan Gerard Horak
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History ,biology ,Ecology ,Game species ,Order Perissodactyla ,Wildlife ,Ungulates ,biology.organism_classification ,Article ,South Africa ,Infectious Diseases ,Cetartiodactyla ,Wildlife parasites ,lcsh:Zoology ,Period (geology) ,Animal Science and Zoology ,Parasitology ,lcsh:QL1-991 ,Superorder ,ComputingMethodologies_COMPUTERGRAPHICS - Abstract
Graphical Abstract, Highlights • The history of wildlife parasitology in South Africa, and to some extent southern Africa, is reviewed. • A brief overview of the early years, starting in the 1890s, is included. • Systematic attempts to study the parasite fauna of each game species since 1970s. • The main findings on protozoan, arthropod and helminth parasites are presented., The history of wildlife parasitology in South Africa, and to some extent southern Africa, is reviewed, giving a brief overview of the early years and following its development from the founding of the Onderstepoort Veterinary Institute in 1908 until the turn of the century. An emphasis is placed on game species. The main findings on protozoan parasites, including those of carnivores, are presented, starting in the 1890s and leading up to the first decade of the 21st century. Important developments with regard to the studies of arthropod and helminth parasites took place during a period of three decades, starting from the 1970s. Because of the sheer volume of work done by parasitologists during this time, this particular part of the overview concentrates on South African authors or authors working in South Africa at the time, and is limited to hosts that are members of the order Perissodactyla and the superorder Cetartiodactyla. more...
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- 2015
12. Delayed Low-Intensity Extracorporeal Shock Wave Therapy Ameliorates Impaired Penile Hemodynamics in Rats Subjected to Pelvic Neurovascular Injury
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Wang, H. S., primary, Ruan, Y., additional, Banie, L., additional, Cui, K., additional, Kang, N., additional, Peng, D., additional, Liu, T., additional, Wang, T., additional, Wang, B., additional, Wang, G., additional, Shindel, A. W., additional, Lin, G., additional, and Lue, T. F., additional more...
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- 2018
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13. 161 Low Intensity Extracorporeal Shock Wave Therapy Activates Endogenous Progenitor/Stem Cells as Indicated by Histone 3 Phosphorylation
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Reed-Maldonado, A.B., primary, Ruan, Y., additional, King, N., additional, Tamaddon, A., additional, Zhou, J., additional, Wang, B., additional, Wang, H.S., additional, Wang, G., additional, Banie, L., additional, Lin, G., additional, and Lue, T.F., additional more...
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- 2018
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14. 071 A Novel Biological Device to Secure and Protect Neophallus Penile Prosthesis Cylinders and the Neourethra following Phalloplasty: Acellular Matrix of Intact Penile Tunica made from Human Penis Specimens following Vaginoplasty
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Garcia, M., primary, Banie, L., additional, and Lin, G., additional
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- 2017
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15. 218 The Rationale for a Urologic Surgical Approach to the Surgical Plane Anterior to Denonvillier's Fascia for Creation of the Neovaginal Cavity during MtoF Vaginoplasty: A Human Cadaveric Histologic Study and Clinical Outcomes
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Garcia, M., primary, Banie, L., additional, Bellringer, J., additional, and Thomas, P., additional
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- 2017
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16. 144 Therapeutic Mechanism of Low Intensity Extracorporeal Shock Wave in Ameliorating Neurogenic Erectile Dysfunction in Bilateral Cavernous Nerve Injury Rat Model
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Li, H., primary, Wu, A., additional, Reed-Maldonado, A., additional, Ning, H., additional, Banie, L., additional, Wang, L., additional, Lee, Y.C., additional, Xin, Z.C., additional, Guo, Y., additional, Lin, G., additional, and Lue, T.F., additional more...
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- 2017
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17. 013 Extracorporeal In-Situ Activation of Penile Progenitor Cells
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Lin, G., primary, Reed-Maldonado, A., additional, Wang, B., additional, Lee, Y.C., additional, Zhou, J., additional, Lu, Z., additional, Wang, G., additional, Banie, L., additional, and Lue, T.F., additional more...
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- 2017
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18. SURVEILLANCE FOR VIRAL AND PARASITIC PATHOGENS IN A VULNERABLE AFRICAN LION (PANTHERA LEO) POPULATION IN THE NORTHERN TULI GAME RESERVE, BOTSWANA
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McDermid, Kimberly R., primary, Snyman, Andrei, additional, Verreynne, Frederick J., additional, Carroll, John P., additional, Penzhorn, Banie L., additional, and Yabsley, Michael J., additional
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- 2017
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19. The impact of co-infections on the haematological profile of East African Short-horn Zebu calves
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Jacobus A.W. Coetzer, Henry K. Kiara, Samuel M. Thumbi, Olivier Hanotte, Maia Lesosky, Mark W. Woolhouse, Banie L. Penzhorn, Amy Jennings, Ilana Van Wyk, Ian Handel, Amelia Goddard, Phil Toye, and B. Mark de C. Bronsvoort more...
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Veterinary medicine ,Nematoda ,CATTLE ,Disease ,Cohort Studies ,Leukocyte Count ,Ticks ,Theileria ,EPIDEMIOLOGY ,Longitudinal Studies ,Pathogen ,WESTERN KENYA ,2. Zero hunger ,Tick-borne disease ,biology ,Coinfection ,Zebu ,3. Good health ,Infectious Diseases ,medicine.anatomical_structure ,Hematocrit ,INFECTIONS ,Tick-Borne Diseases ,platelets ,Female ,Haemonchus contortus ,Research Article ,HAEMONCHUS-CONTORTUS ,Trypanosoma ,Anaplasma ,Theileria parva ,Parasitic Diseases, Animal ,Babesia ,Cattle Diseases ,calves ,White blood cell ,medicine ,Animals ,DAIRY FARMS ,packed cell volume ,LINKED-IMMUNOSORBENT-ASSAY ,Platelet Count ,co-infections ,TRYPANOSOMA-CONGOLENSE ,biology.organism_classification ,medicine.disease ,Kenya ,THEILERIA-PARVA ,Animals, Newborn ,Concomitant ,TICK-BORNE DISEASES ,Animal Science and Zoology ,Parasitology ,Cattle ,Gram-Negative Bacterial Infections ,white blood cell count - Abstract
SUMMARYThe cumulative effect of co-infections between pathogen pairs on the haematological response of East African Short-horn Zebu calves is described. Using a longitudinal study design a stratified clustered random sample of newborn calves were recruited into the Infectious Diseases of East African Livestock (IDEAL) study and monitored at 5-weekly intervals until 51 weeks of age. At each visit samples were collected and analysed to determine the infection status of each calf as well as their haematological response. The haematological parameters investigated included packed cell volume (PCV), white blood cell count (WBC) and platelet count (Plt). The pathogens of interest included tick-borne protozoa and rickettsias, trypanosomes and intestinal parasites. Generalized additive mixed-effect models were used to model the infectious status of pathogens against each haematological parameter, including significant interactions between pathogens. These models were further used to predict the cumulative effect of co-infecting pathogen pairs on each haematological parameter. The most significant decrease in PCV was found with co-infections of trypanosomes and strongyles. Strongyle infections also resulted in a significant decrease in WBC at a high infectious load. Trypanosomes were the major cause of thrombocytopenia. Platelet counts were also affected by interactions between tick-borne pathogens. Interactions between concomitant pathogens were found to complicate the prognosis and clinical presentation of infected calves and should be taken into consideration in any study that investigates disease under field conditions. more...
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- 2014
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20. Vector-Borne Diseases - constant challenge for practicing veterinarians
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Patrick Bourdeau, Ronan Fitzgerald, Uwe Truyen, Ángel Sainz, Dwight D. Bowman, Babara Kohn, Luís Cardoso, Gioia Capelli, Bob Rees, Banie L. Penzhorn, Cláudio Mendão, Lluís Ferrer, Susan Shaw, Michael R. Lappin, Joe Hostetler, Gilles Bourdoiseau, Diego Gatti, Sarah Weston, Edward B. Breitschwerdt, Torsten J Naucke, Domenico Otranto, Sung-Shik Shin, Laia Solano-Gallego, Reinhard K. Straubinger, Susan H. Little, Cristiano von Simson, Gad Baneth, Gaetano Oliva, Thierry Demonceau, Michael J. Day, Filipe Dantas-Torres, Alexander J. Trees, Xavier Roura, Bruce Kilmer, Lourdes Mottier, Martin Pfeffer, Jean-Pierre Dedet, Peter J. Irwin, Ricardo G. Maggi, Klemens Krieger, Norbert Mencke, Montserrat Tarancón Mingote, Guadalupe Miró, Susanne Siebert, Volkhard A. J. Kempf, Stefan Pachnicke, Rebecca J. Traub, Dorothee Stanneck, and Gerhard Dobler more...
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Veterinary Medicine ,Veterinary medicine ,education.field_of_study ,Economic growth ,business.industry ,Parasitic Diseases, Animal ,Population ,Bacterial Infections ,Disease ,Family life ,lcsh:Infectious and parasitic diseases ,Dogs ,Infectious Diseases ,Vector (epidemiology) ,Zoonoses ,Ehrlichiosis (canine) ,Animals ,Medicine ,Parasitology ,lcsh:RC109-216 ,Dog Diseases ,business ,education ,Letter to the Editor - Abstract
The human-animal bond has been a fundamental feature of mankind's history for millennia. The first, and strongest of these, man's relationship with the dog, is believed to pre-date even agriculture, going back as far as 30,000 years. It remains at least as powerful today. Fed by the changing nature of the interactions between people and their dogs worldwide and the increasing tendency towards close domesticity, the health of dogs has never played a more important role in family life. Thanks to developments in scientific understanding and diagnostic techniques, as well as changing priorities of pet owners, veterinarians are now able, and indeed expected, to play a fundamental role in the prevention and treatment of canine disease, including canine vector-borne diseases (CVBDs). The CVBDs represent a varied and complex group of diseases, including anaplasmosis, babesiosis, bartonellosis, borreliosis, dirofilariosis, ehrlichiosis, leishmaniosis, rickettsiosis and thelaziosis, with new syndromes being uncovered every year. Many of these diseases can cause serious, even life-threatening clinical conditions in dogs, with a number having zoonotic potential, affecting the human population. Today, CVBDs pose a growing global threat as they continue their spread far from their traditional geographical and temporal restraints as a result of changes in both climatic conditions and pet dog travel patterns, exposing new populations to previously unknown infectious agents and posing unprecedented challenges to veterinarians. In response to this growing threat, the CVBD World Forum, a multidisciplinary group of experts in CVBDs from around the world which meets on an annual basis, gathered in Nice (France) in 2011 to share the latest research on CVBDs and discuss the best approaches to managing these diseases around the world. As a result of these discussions, we, the members of the CVBD Forum have developed the following recommendations to veterinarians for the management of CVBDs. more...
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- 2012
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21. Preface to the special issue of the International Congress on Parasites of Wildlife, 15–18 September 2014, Kruger National Park, South Africa
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Penzhorn, Banie L., primary and Smit, Nico J., additional
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- 2015
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22. Feline babesiosis in South Africa: a review
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L.S. Jacobson, Banie L. Penzhorn, and Tanya Schoeman
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Hemolytic anemia ,Anemia, Hemolytic ,Anemia ,Babesia ,Anorexia ,Biology ,Disease Vectors ,Cat Diseases ,General Biochemistry, Genetics and Molecular Biology ,Diagnosis, Differential ,Lethargy ,South Africa ,History and Philosophy of Science ,Babesiosis ,parasitic diseases ,medicine ,Animals ,CATS ,General Neuroscience ,medicine.disease ,biology.organism_classification ,Mycoplasma haemofelis ,Alanine transaminase ,Immunology ,biology.protein ,Cats ,medicine.symptom - Abstract
Babesia felis, originally identified in wild cats in the Sudan, was subsequently found to cause clinical disease in domestic cats. Although babesiosis in domestic cats has been reported sporadically from various countries, as a significant disease it appears to be a distinctly South African phenomenon. Apart from an inland focus, feline babesiosis is reported regularly only from coastal regions. The infection is assumed to be tick-borne, but the vector has not been identified. Feline babesiosis tends to be an afebrile, chronic, low-grade disease. The most frequently reported complaints by owners are anorexia and lethargy. The main clinical findings are anemia, depression, and occasionally icterus. Concurrent infections (e.g., Mycoplasma haemofelis, FeLV, FIV) may contribute to the clinical picture. Laboratory findings commonly include regenerative anemia, elevation of alanine transaminase (but not alkaline phosphatase) and total bilirubin concentrations, and a variety of electrolyte disturbances. Secondary immune-mediated hemolytic anemia can be seen occasionally. Drugs effective against other Babesia species give variable and questionable results. The drug of choice is primaquine phosphate, which effects a clinical cure but does not sterilize the infection. Repeated or chronic therapy may be required. more...
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- 2004
23. Ornithodoros peringueyi (Argasidae) and Xenopsylla trispinis (Siphonaptera), probable intermediate hosts of Hepatozoon atticorae of the South African Cliff Swallow, Hirundo spilodera
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Banie L. Penzhorn, Gordon F. Bennett, and Roy A. Earlé
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biology ,Hirundo spilodera ,Ecology ,digestive, oral, and skin physiology ,Argasidae ,Zoology ,biology.organism_classification ,Hirundinidae ,Hepatozoon ,Pulicidae ,biology.animal ,Hemolymph ,Animal Science and Zoology ,Xenopsylla ,Ecology, Evolution, Behavior and Systematics ,Ornithodoros - Abstract
Developing and mature oocysts of Hepatozoon atticorae of the Hirundinidae were detected in the haemolymph of 60% f the Ornithodoros peringueyi (Argasidae) and 25% of the Xenopsylla trispinis (Pulicidae) that occurred in a colony of South African Cliff Swallows (Hirundo spilodera). Fifty-seven percent of the adult birds harboured the hepatozoan, but none of the eight immature swallows examined were infected. It is speculated that the adult birds become infected by ingesting infected arthropods while preening. more...
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- 1992
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24. The impact of co-infections on the haematological profile of East African Short-horn Zebu calves
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VAN WYK, ILANA CONRADIE, primary, GODDARD, AMELIA, additional, DE C. BRONSVOORT, B. MARK, additional, COETZER, JACOBUS A. W., additional, HANDEL, IAN G., additional, HANOTTE, OLIVIER, additional, JENNINGS, AMY, additional, LESOSKY, MAIA, additional, KIARA, HENRY, additional, THUMBI, SAM M., additional, TOYE, PHIL, additional, WOOLHOUSE, MARK W., additional, and PENZHORN, BANIE L., additional more...
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- 2013
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25. Thérapie cellulaire pour maladie de Lapeyronie : effet des cellules souches dérivées des adipocytes sur un model animal
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Ferretti, L., primary, Qiu, X., additional, Fandel, T., additional, Zhang, H., additional, Banie, L., additional, Lin, G., additional, and Lue, T., additional
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- 2012
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26. 707 Tunica albuginea allograft: A new rat model of Peyronie's disease with penile curvature and cartilage formation
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Ferretti, L., primary, Fandel, T., additional, Qiu, X., additional, Banie, L., additional, Lin, G., additional, Droupy, S., additional, and Lue, T.F., additional
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- 2012
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27. 1064 PENTOXIFYLLINE PROMOTES RECOVERY OF ERECTILE FUNCTION IN A RAT MODEL OF POST-PROSTATECTOMY ERECTILE DYSFUNCTION
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Albersen, M., primary, Fandel, T.M., additional, Zhang, H., additional, Banie, L., additional, Lin, G., additional, Lin, C.S., additional, and Lue, T.F., additional
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- 2011
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28. POSTER VIEWING SESSION - TRANSLATIONAL RESEARCH
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Fields, T., primary, Neri, Q. V., additional, Hu, J. C. Y., additional, Rosenwaks, Z., additional, Palermo, G. D., additional, Huang, Z., additional, Andonov, M., additional, Enciso, M., additional, Wells, D., additional, Garcia, M., additional, Ohta, A., additional, Valley, J., additional, Banie, L., additional, Espineda, S., additional, Boscardin, J., additional, Lue, T., additional, Wu, M., additional, Gaikwad, S. A., additional, Garrido, N., additional, Alama, P., additional, Labarta, E., additional, Privitera, L., additional, Remohi, J., additional, Talevi, R., additional, Fiorentino, I., additional, Mollo, V., additional, Barbato, V., additional, Gualtieri, R., additional, Seshadri, S., additional, Sunkara, S. K., additional, Hamooda, H., additional, Khalaf, Y., additional, El-Toukhy, T., additional, Hashimoto, S., additional, Suzuki, N., additional, Ishizuka, B., additional, Amo, A., additional, Yamochi, T., additional, Hosoi, Y., additional, Morimoto, Y., additional, Otsuki, J., additional, Nagai, Y., additional, Yasmin, L., additional, Lopata, A., additional, Sankai, T., additional, Takahashi, K., additional, Nagaba, M., additional, and Mukaida, T., additional more...
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- 2011
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29. 13 LIGHT-INDUCED DIELECTROPHORESIS FOR THE IDENTIFICATION OF VIABLE, OPTIMAL QUALITY SPERM WITHIN AN UNWASHED SEMEN SAMPLE: FEASIBILITY AND SAFETY STUDIES
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Garcia, M.M., primary, Ohta, A.T., additional, Banie, L., additional, Valley, J.K., additional, Boscardin, W.J., additional, Lue, T.F., additional, and Wu, M.C., additional
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- 2010
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30. Identification of a Novel Babesia sp. from a Sable Antelope ( Hippotragus niger Harris, 1838)
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Oosthuizen, Marinda C., primary, Zweygarth, Erich, additional, Collins, Nicola E., additional, Troskie, Milana, additional, and Penzhorn, Banie L., additional
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- 2008
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31. Babesia bicornis sp. nov. and Theileria bicornis sp. nov.: Tick-Borne Parasites Associated with Mortality in the Black Rhinoceros ( Diceros bicornis )
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Nijhof, Ard M., primary, Penzhorn, Banie L., additional, Lynen, Godelieve, additional, Mollel, Johnson O., additional, Morkel, Pete, additional, Bekker, Cornelis P. J., additional, and Jongejan, Frans, additional more...
- Published
- 2003
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32. Ornithodoros peringueyi (Argasidae) and Xenopsylla trispinis (Siphonaptera), probable intermediate hosts of Hepatozoon atticorae of the South African Cliff Swallow, Hirundo spilodera
- Author
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Bennett, Gordon F., primary, Earlé, Roy A., additional, and Penzhorn, Banie L., additional
- Published
- 1992
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33. Identification of a Novel Babesiasp. from a Sable Antelope (Hippotragus nigerHarris, 1838)
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Oosthuizen, Marinda C., Zweygarth, Erich, Collins, Nicola E., Troskie, Milana, and Penzhorn, Banie L.
- Abstract
ABSTRACTBabesiosis in a sable antelope (Hippotragus nigerHarris, 1838) was first reported in 1930; the parasite was named Babesia irvinesmithi. Recently, specimens from an adult sable that presented with a sudden onset of disease and that subsequently died during immobilization were submitted for molecular characterization. Microscopic examination of thin blood smears revealed the presence of small piroplasms. DNA was extracted from blood samples; the V4 variable region of the 18S rRNA gene was amplified and analyzed using the reverse line blot (RLB) assay. Amplicons did not hybridize with any of the Babesiaor Theileriaspecies-specific probes present on the blot and hybridized only with a Babesiaor Theileriagenus-specific probe, suggesting the presence of a novel species. The full-length 18S rRNA gene sequence was obtained and aligned with published sequences of related genera, and phylogenetic trees were constructed. Sequence similarity analyses indicated that a Babesiaspecies, designated Babesiasp. (sable), was present. The sequence showed its highest similarity to B. orientalisand to an unnamed Babesiaspecies previously detected in bovine samples. The latter was later established to be Babesia occultans. A Babesiasp. (sable)-specific RLB oligonucleotide probe was designed and used to screen 200 South African sable samples, but so far, no other sample has been found to be positive for the presence of Babesiasp. (sable) DNA. In summary, we identified a novel piroplasm parasite from a sable antelope that died from an unknown illness. While the parasite was observed in blood smears, there is no direct evidence that it was the cause of death. more...
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- 2008
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34. Babesia bicornissp. nov. and Theileria bicornissp. nov.: Tick-Borne Parasites Associated with Mortality in the Black Rhinoceros (Diceros bicornis)
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Nijhof, Ard M., Penzhorn, Banie L., Lynen, Godelieve, Mollel, Johnson O., Morkel, Pete, Bekker, Cornelis P. J., and Jongejan, Frans
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ABSTRACTA novel Babesiaspecies, designated Babesia bicornissp. nov., was identified in three black rhinoceroses (Diceros bicornis) that died in wildlife areas in Tanzania and South Africa. Screening of black rhinoceroses in South Africa revealed, in addition to B. bicornis, a second parasite, designated Theileria bicornissp. nov. more...
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- 2003
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35. LIGHT-INDUCED DIELECTROPHORESIS FOR THE IDENTIFICATION OF VIABLE, OPTIMAL QUALITY SPERM WITHIN AN UNWASHED SEMEN SAMPLE: FEASIBILITY AND SAFETY STUDIES.
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Garcia, M. M., Ohta, A. T., Banie, L., Valley, J. K., Boscardin, W. J., Lue, T. F., and Wu, M. C.
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DIELECTROPHORESIS , *DIELECTRICS , *ELECTROPHORESIS , *SPERMATOZOA , *GERM cells , *SEMEN - Abstract
Introduction and Purpose: ICSI (intracytoplasmic sperm injection) outcomes are suboptimal. There is currently no means by which to assess sperm quality in sperm reserved for ICSI. Herein, we introduce application of a novel, non-invasive, light-based dielectrophoresis technology, termed Optoelectronic Tweezers (OET) to distinguish among viable and non-viable sperm, completely independent of sperm motility. In pilot studies motile (viable) sperm are spontaneously attracted to, and sperm suspected of being dead are weakly repulsed to projected OET fields. In the present work, we compare the predictive value OET to identify viable nonmotile sperm to the toxic, but reliable, Trypan Blue assay. We also hypothesize that the magnitude of attraction is itself a continuous outcome predictor of viability ("quality"). The magnitude and density distribution of individual viable sperm responses is assessed. Using COMET (single cell gel DNA fragmentation electrophoresis) assay, we assess whether OET results in a detectable increase in DNA fragmentation damage to sperm. Materials and Methods: Six healthy subjects provided fresh ejaculated semen samples. Specimens were suspended in minimally conductive isotonic sucrose solution and incubated with Trypan Blue dye, for simultaneous OET and Trypan assay. From each subject, under DIC microscopy, 25 non-motile sperm were identified by OET as 'viable', and 25 were identified as 'non-viable'. Maximal attraction or repulsion was measured for each sperm. Thereafter, under Brightfield view, Trypan staining status was assessed and recorded. Motile sperm served as positive assay controls. To assess potential sperm DNA damage, alkaline COMET assay was performed on sperm aliquots exposed/not-exposed to: OET assay at 1x and 2x voltage, and OET medium only. Positive and negative assay controls were used. Results: OET assay predicted viability among all motile sperm in complete agreement (100% sensitivity and specificity) with Trypan assay. Among non-motile sperm predicted by OET to be dead (N = 150), there was also 100% agreement with Trypan assay. Among nonmotile sperm predicted by OET to be viable (N = 150), all (100%) were classified as viable by Trypan assay. The only inter-assay disagreement concerned 18/150 (11%) sperm classified as "dead" by OET but "alive" by Trypan. Overall inter-assay agreement was 94%; OET had 89% sensitivity and 100% specificity relative to Trypan assay. Magnitude of OET response among viable sperm was right-skewed (p < 0.00001), and left-skewed among nonviable sperm (p < 0.0021). No increase in DNA fragmentation was detectable by COMET (p>>0.05). Conclusions: A sperm's absolute OET response (+ [attraction] or [repulsion] to the OET field) predicts absolute sperm viability among completely non-motile sperm, independent of motility, and with sensitivity and specificity comparable (and possibly superior) to Trypan Blue assay. The wide distribution of OET responses (i.e. magnitude of response) among viable sperm is non-normal and right-skewed, similar to the distribution predicted by biological natural selection models. COMET assay results suggest OET does not cause DNA fragmentation to the sperm genome. While in vivo studies are necessary to confirm that OET dos not harm sperm, this work suggests that, at a minimum, OET could be used to identify viable sperm for ICSI when sperm motility is minimal or completely absent. It is possible that relative OET response predicts relative quality among viable sperm. [ABSTRACT FROM AUTHOR] more...
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- 2010
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36. Effects of prolonged vaginal distension and β-aminopropionitrile on urinary continence and urethral structure.
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Wang G, Lin G, Zhang H, Qiu X, Ning H, Banie L, Fandel T, Albersen M, Lue TF, Lin CS, Wang, Guifang, Lin, Guiting, Zhang, Haiyang, Qiu, Xuefeng, Ning, Hongxiu, Banie, Lia, Fandel, Thomas, Albersen, Maarten, Lue, Tom F, and Lin, Ching-Shwun more...
- Abstract
Objective: To investigate the effects of prolonged vaginal distension and β-aminopropionitrile (BAPN) on the urinary patterns and urethral structure in female virgin rats.Methods: A total of 21 female virgin rats were randomly divided into 3 groups of 7 rats each. The control group received no intervention; the vaginal distension (VD) group was treated with prolonged VD by balloon inflation; and the VD plus BAPN group was treated with VD plus intraperitoneal injection of 150 mg/kg of BAPN every 3 days. Three weeks later, all the rats underwent conscious cystometric analysis and were then killed for histologic analysis of the urethra.Results: Conscious cystometry identified 0, 3, and 5 rats in the control, VD, and VD plus BAPN groups with an abnormal voiding pattern, respectively. The urethral collagen content was significantly lower in the VD and VD plus BAPN rats compared with the control rats. The urethral elastic fibers were disorganized and shorter in the VD and VD plus BAPN rats and were fragmented and lacking the intermuscle connections in the VD plus BAPN rats. The urethral striated muscle fibers were shorter and more widely spaced in the VD and VD plus BAPN rats than in the control rats. Additionally, those in the VD plus BAPN group exhibited an abnormal wavy shape suggestive of a lack of architectural support.Conclusion: Prolonged vaginal distension caused urodynamic changes and histologic abnormalities in the urethra, including reduced collagen content, fragmented elastic fibers, and sparsely arranged and shortened striated muscle fibers. BAPN appears to interfere with the restoration of collagen and elastic fibers. [ABSTRACT FROM AUTHOR] more...- Published
- 2011
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37. Age-Related Changes in Urethral Structure and Responds to Injury: Single-Cell Atlas of a Rat Model of Vaginal Birth Injury induced Stress Urinary Incontinence.
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Zhao L, Xing E, Bai T, Cao T, Wang G, Banie L, Lin G, Tang Y, and Lue T
- Abstract
Stress urinary incontinence (SUI) greatly affects the daily life of numerous women and is closely related to a history of vaginal delivery and aging. We used vaginal balloon dilation to simulate vaginal birth injury in young and middle-aged rats to produce a SUI animal model, and found that young rats restored urethral structure and function well, but not the middle-aged rats. To identify the characteristics of cellular and molecular changes in the urethral microenvironment during the repair process of SUI. We profiled 51,690 individual female rat urethra cells from 24 and 48 weeks old, with or without simulated vaginal birth injury. Cell interaction analysis showed that signal networks during repair process changed from resting to active, and aging altered the distribution but not the overall level of cell interaction in the repair process. Similarity analysis showed that muscle, fibroblasts, and immune cells underwent large transcriptional changes during aging and repair. In middle-aged rat s , cell senescence occurs mainly in the superficial and middle urothelium due to cellular death and shedding, and the basal urothelium expressed many Senescence-Associated Secretory Phenotype (SASP) genes. In conclusion, we established the aging and vaginal balloon dilation (VBD) model of female urethral cell anatomy and the signal network landscape, which provides an insight into the normal or disordered urethra repair process and the scientific basis for developing novel SUI therapies., Competing Interests: Additional Declarations: There is NO Competing Interest. The authors declare no competing interests. more...
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- 2024
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38. Microenergy acoustic pulse therapy restores urethral wall integrity and continence in a rat model of female stress incontinence.
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Tan Y, Reed-Maldonado AB, Wang G, Banie L, Peng D, Zhou F, Chen Y, Wang Z, Lin G, and Lue TF
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- Acoustics, Aminopropionitrile, Animals, Disease Models, Animal, Female, Rats, Rats, Sprague-Dawley, Urethra, Urinary Incontinence, Stress
- Abstract
Objective: To determine the outcomes and mechanisms of microenergy acoustic pulse (MAP) therapy in an irreversible rat model of female stress urinary incontinence., Materials and Methods: Twenty-four female Sprague-Dawley rats were randomly assigned into four groups: sham control (sham), vaginal balloon dilation and ovariectomy (VBDO), VBDO + β-aminopropionitrile (BAPN), and VBDO + β-aminopropionitrile treated with MAP (MAP). MAP therapy was administered twice per week for 4 weeks. After a 1-week washout period, all 24 rats were evaluated with functional and histological studies. The urethral vascular plexus was examined by immunofluorescence staining with antibodies against collagen IV and von Willebrand factor (vWF). The urethral smooth muscle stem/progenitor cells (uSMPCs) were isolated and functionally studied in vivo and in vitro., Results: Functional study with leak point pressure (LPP) measurement showed that the MAP group had significantly higher LPPs compared to VBDO and BAPN groups. MAP ameliorated the decline in urethral wall thickness and increased the amount of extracellular matrix within the urethral wall, especially in the urethral and vaginal elastic fibers. MAP also improved the disruption of the urethral vascular plexus in the treated animals. In addition, MAP enhanced the regeneration of urethral and vaginal smooth muscle, and uSMPCs could be induced by MAP to differentiate into smooth muscle and neuron-like cells in vitro., Conclusion: MAP appears to restore urethral wall integrity by increasing muscle content in the urethra and the vagina and by improving the urethral vascular plexus and the extracellular matrix., (© 2022 Wiley Periodicals LLC.) more...
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- 2022
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39. Enhanced Myogenesis by Silencing Myostatin with Nonviral Delivery of a dCas9 Ribonucleoprotein Complex.
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Chen Y, Banie L, Breyer BN, Tan Y, Wang Z, Zhou F, Wang G, Lin G, Liu J, Qi LS, and Lue TF
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- Animals, Female, Gene Editing, Glycogen Synthase Kinase 3 beta genetics, Humans, Rats, Ribonucleoproteins genetics, CRISPR-Cas Systems, Muscle Development genetics, Muscle Development physiology, Myostatin genetics, Myostatin metabolism, Urinary Incontinence, Stress genetics, Urinary Incontinence, Stress metabolism
- Abstract
Stress urinary incontinence (SUI) and pelvic floor disorder (PFD) are common conditions with limited treatment options in women worldwide. Regenerative therapy to restore urethral striated and pelvic floor muscles represents a valuable therapeutic approach. We aim to determine the CRISPR interference-mediated gene silencing effect of the nonviral delivery of nuclease-deactivated dCas9 ribonucleoprotein (RNP) complex on muscle regeneration at the cellular and molecular level. We designed four myostatin ( MSTN )-targeting sgRNAs and transfected them into rat myoblast L6 cells together with the dCas9 protein. Myogenesis assay and immunofluorescence staining were performed to evaluate muscle differentiation, while CCK8 assay, cell cycle assay, and 5-ethynyl-2'-deoxyuridine staining were used to measure muscle proliferation. Reverse transcription-polymerase chain reaction and Western blotting were also performed to examine cellular signaling. Myogenic factors (including myosin heavy chain, MSTN, myocardin, and serum response factor) increased significantly after day 5 during myogenesis. MSTN was efficiently silenced after transfecting the dCas9 RNP complex, which significantly promoted more myotube formation and a higher fusion index for L6 cells. In cellular signaling, MSTN repression enhanced the expression of MyoG and MyoD, phosphorylation of Smad2, and the activity of Wnt1/GSK-3β/β-catenin pathway. Moreover, MSTN repression accelerated L6 cell growth with a higher cell proliferation index as well as a higher expression of cyclin D1 and cyclin E. Nonviral delivery of the dCas9 RNP complex significantly promoted myoblast differentiation and proliferation, providing a promising approach to improve muscle regeneration for SUI and PFD. Further characterization and validation of this approach in vivo are needed. more...
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- 2022
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40. Microenergy acoustic pulses promotes muscle regeneration through in situ activation of muscle stem cells.
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Zhang H, Kim HT, Feeley BT, Lin G, Lue TF, Liu M, Banie L, and Liu X
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- Acoustics, Adipogenesis, Animals, Cell Differentiation, Mice, Mice, Inbred C57BL, Muscle, Skeletal, Regeneration, Stem Cells physiology, Endothelial Cells, Myoblasts
- Abstract
Microenergy acoustic pulses (MAP) is a modified low-intensity extracorporeal shock wave therapy that currently used for treating musculoskeletal disorders. However, its function on muscle regeneration after ischemia-reperfusion injury (IRI) remains unknown. This study aimed to explore the effect of MAP on muscle injury after IRI and its underlying mechanisms. Ten-week-old C57BL/6J mice underwent unilateral hindlimb IRI followed with or without MAP treatment. Wet weight of tibialis anterior muscles at both injury and contralateral sides were measured followed with histology analysis at 3 weeks after IRI. In in vitro study, the myoblasts, endothelial cells and fibro-adipogenic progenitors (FAP) were treated with MAP. Cell proliferation and differentiation were assessed, and related gene expressions were measured by real-time PCR. Our results showed that MAP significantly increased the muscle weight and centrally nucleated regenerating muscle fiber size along with a trend in activating satellite cells. In vitro data indicated that MAP promoted myoblast proliferation and differentiation and endothelial cells migration. MAP also induced FAP brown/beige adipogenesis, a promyogenic phenotype of FAPs. Our findings demonstrate the beneficial function of MAP in promoting muscle regeneration after IR injury by inducing muscle stem cells proliferation and differentiation., (© 2021 Orthopaedic Research Society. Published by Wiley Periodicals LLC.) more...
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- 2022
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41. Microenergy acoustic pulse therapy restores function and structure of pelvic floor muscles after simulated birth injury.
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Lin G, Van Kuiken M, Wang G, Banie L, Tan Y, Zhou F, Wang Z, Chen Y, Zhang Y, and Lue TF
- Abstract
Background: The mechanisms of the microenergy acoustic pulse (MAP) therapy on restoring structure and function of pelvic floor muscles (PFM) after simulated birth injury are not well understood., Methods: A total 24 female Sprague-Dawley rats were randomly grouped into sham control (sham), vaginal balloon dilation and ovariectomy (VBDO), VBDO + β-aminopropionitrile (BAPN, an irreversible LOX inhibitor), and VBDO + BAPN and treated with MAP (n=6 in each group). The MAP therapy was administered 2 times per week for 4 weeks with 1-week washout, the functional and histological studies were conducted in all 24 rats. The viscoelastic behavior of the PFM, including iliococcygeus (IC) and pubococcygeus (PC), was examined with a biomechanical assay. The structure of the PFM was assessed by immunofluorescence and Masson's trichrome staining., Results: The leak point pressure (LPP) assay demonstrated that the MAP therapy group had higher LPPs compared to that of VBDO and BAPN groups. In the sham group, the muscular stiffness (K) of IC muscle was significantly higher than that of PC muscle while the pelvic floor muscle rebound activity (MRA) of PC muscle was stronger than that of IC muscle (291.26±45.33 and 241.18±14.23 N/cm
2 , respectively). Both VBDO and BAPN decreased the MRA and increased the K in both IC and PC. Histologic examination revealed increased fibrous tissue (collagen) and degeneration of muscle fibers in both VBDO and BAPN groups. MAP therapy significantly reduced the collagen content and improved the architecture of muscle fibers., Conclusions: MAP appears to restore the structure and function of PFM by regenerating muscular fibers and improving biomechanical properties in an animal model of simulated birth injury., Competing Interests: Conflicts of Interest: All authors have completed the ICMJE uniform disclosure form (available at https://tau.amegroups.com/article/view/10.21037/tau-22-30/coif). TFL serves as an unpaid Editor-in-Chief for Translational Andrology and Urology. GL serves as an unpaid Editorial Board Member from August 2020 to July 2022 for Translational Andrology and Urology. YZ is the founder and CEO of HillMed Inc. and BrainHealth Tech Inc. TFL is a consultant, co-founder and investor of Acoustic Wave Cell Therapy, Inc. The other authors have no conflicts of interest to declare., (2022 Translational Andrology and Urology. All rights reserved.) more...- Published
- 2022
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42. Regenerating Urethral Striated Muscle by CRISPRi/dCas9-KRAB-Mediated Myostatin Silencing for Obesity-Associated Stress Urinary Incontinence.
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Yuan H, Ruan Y, Tan Y, Reed-Maldonado AB, Chen Y, Zhao D, Wang Z, Zhou F, Peng D, Banie L, Wang G, Liu J, Lin G, Qi LS, and Lue TF
- Subjects
- Animals, CRISPR-Associated Proteins genetics, CRISPR-Cas Systems genetics, Clustered Regularly Interspaced Short Palindromic Repeats genetics, Clustered Regularly Interspaced Short Palindromic Repeats physiology, Computational Biology methods, Female, Gene Editing methods, Gene Silencing physiology, Genomics methods, Muscle, Skeletal metabolism, Muscle, Striated physiology, Myoblasts metabolism, Myostatin metabolism, Obesity complications, Pelvic Floor, Rats, Rats, Zucker, Regeneration physiology, Urethra metabolism, Urethra physiology, Urinary Bladder, Urinary Incontinence, Stress etiology, RNA, Guide, CRISPR-Cas Systems, Guided Tissue Regeneration methods, Muscle, Striated metabolism, Myostatin genetics
- Abstract
Overweight females are prone to obesity-associated stress urinary incontinence (OA-SUI), and there are no definitive medical therapies for this common urologic condition. This study was designed to test the hypothesis that regenerative therapy to restore urethral striated muscle (stM) and pelvic floor muscles might represent a valuable therapeutic approach. For the in vitro experiment, single-guide RNAs targeting myostatin ( MSTN ) were used for CRISPRi/dCas9-Kruppel associated box (KRAB)-mediated gene silencing. For the in vivo experiment, a total of 14 female lean ZUC-Lepr
fa 186 and 14 fatty ZUC-Leprfa 185 rats were used as control and CRISPRi-MSTN treated groups, respectively. The results indicated that lentivirus-mediated expression of MSTN CRISPRi/dCas9-KRAB caused sustained downregulation of MSTN in rat L6 myoblast cells and significantly enhanced myogenesis in vitro . In vivo , the urethral sphincter injection of lentiviral-MSTN sgRNA and lentiviral-dCas9-KRAB significantly increased the leak point pressure, the thickness of the stM layer, the ratio of stM to smooth muscle, and the number of neuromuscular junctions. Downregulation of MSTN with CRISPRi/dCas9-KRAB-mediated gene silencing significantly enhanced myogenesis in vitro and in vivo. It also improved urethral continence in the OA-SUI rat model. more...- Published
- 2020
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43. Exosome Released From Schwann Cells May Be Involved in Microenergy Acoustic Pulse-Associated Cavernous Nerve Regeneration.
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Peng D, Reed-Maldonado AB, Zhou F, Tan Y, Yuan H, Banie L, Wang G, Tang Y, He L, Lin G, and Lue TF
- Subjects
- Acoustics, Animals, Humans, Male, Nerve Regeneration, Rats, Rats, Sprague-Dawley, Schwann Cells, Exosomes
- Abstract
Background: Neurogenic erectile dysfunction (ED) is often refractory to treatment because of insufficient functional nerve recovery after injury or insult. Noninvasive mechano-biological intervention, such as microenergy acoustic pulse (MAP), low-intensity pulsed ultrasound, and low-intensity extracorporeal shockwave treatment, is an optimal approach to stimulate nerve regeneration., Aim: To establish a new model in vitro to simulate nerve injury in neurogenic ED and to explore the mechanisms of MAP in vitro., Methods: Sprague-Dawley rats were used to isolate Schwann cells (SCs), major pelvic ganglion (MPG), and cavernous nerve with MPG (CN/MPG). SCs were then treated with MAP (0.033 mJ/mm
2 , 1 Hz, 100 pulses), and SC exosomes were isolated. The MPG and CN/MPG were treated with MAP (0.033 mJ/mm2 , 1 Hz) at different dosages (25, 50, 100, 200, or 300 pulses) or exosomes derived from MAP-treated SCs in vitro., Outcomes: Neurite growth from the MPG fragments and CN was photographed and measured. Expression of neurotropic factors (brain-derived neurotrophic factor, nerve growth factor, and neurotrophin-3) was checked., Results: Neurite outgrowth from MPG and CN/MPG was enhanced by MAP in a dosage response manner, peaking at 100 pulses. MAP promoted SC proliferation, neurotropic factor (brain-derived neurotrophic factor, nerve growth factor, and neurotrophin-3) expression, and exosome secretion. SC-derived exosomes significantly enhanced neurite outgrowth from MPG in vitro., Clinical Implications: MAP may have utility in the treatment of neurogenic ED by SC-derived exosomes., Strength & Limitations: We confirmed that MAP enhances penile nerve regeneration through exsomes. Limitations of this study include that our study did not explore the exact mechanisms of how MAP increases SC exosome secretion nor whether MAP modulates the content of exosomes., Conclusion: This study revealed that neurite outgrowth from MPG was enhanced by MAP and by SC-derived exosomes which were isolated after MAP treatment. Our findings indicate that one mechanism by which MAP induces nerve regeneration is by stimulation of SCs to secrete exosomes. Peng D, Reed-Maldonado AB, Zhou F, et al. Exosome Released From Schwann Cells May Be Involved in Microenergy Acoustic Pulse-Associated Cavernous Nerve Regeneration. J Sex Med 2020;17:1618-1628., (Copyright © 2020 International Society for Sexual Medicine. Published by Elsevier Inc. All rights reserved.) more...- Published
- 2020
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44. Delayed Treatment With Low-intensity Extracorporeal Shock Wave Therapy in an Irreversible Rat Model of Stress Urinary Incontinence.
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Zhang X, Ruan Y, Wu AK, Zaid U, Villalta JD, Wang G, Banie L, Reed-Maldonado AB, Lin G, and Lue TF
- Subjects
- Aminopropionitrile, Animals, Deoxyuridine analogs & derivatives, Deoxyuridine metabolism, Dilatation, Disease Models, Animal, Female, Muscle, Smooth innervation, Neuromuscular Junction pathology, Nitric Oxide Synthase metabolism, Random Allocation, Rats, Rats, Sprague-Dawley, Stem Cells metabolism, Stem Cells pathology, Tyrosine 3-Monooxygenase metabolism, Urethra innervation, Urethra pathology, Urinary Incontinence, Stress etiology, Urinary Incontinence, Stress metabolism, Urinary Incontinence, Stress pathology, Urothelium pathology, Vagina pathology, Extracorporeal Shockwave Therapy methods, Muscle, Skeletal pathology, Muscle, Smooth pathology, Nerve Fibers enzymology, Urinary Incontinence, Stress therapy
- Abstract
Objective: To determine the outcomes and mechanisms of delayed low-intensity extracorporeal shock wave therapy (Li-ESWT) in a rat model of irreversible stress urinary incontinence (SUI)., Materials and Methods: Twenty-four female Sprague-Dawley rats were randomly assigned into 3 groups: sham control, vaginal balloon dilation + β-aminopropionitrile (BAPN; SUI group), and vaginal balloon dilation + BAPN + treatment with Li-ESWT (SUI-Li-ESWT group). An irreversible SUI model was developed by inhibiting the urethral structural recovery with BAPN daily for 5 weeks. Thereafter, in the SUI-Li-ESWT group, Li-ESWT was administered twice per week for 2 weeks. After a 1-week washout, all 24 rats were evaluated with functional and histologic studies at 17 weeks of age. Endogenous progenitor cells were detected via the EdU-labeling method., Results: Functional analysis with leak point pressure testing showed that the SUI-Li-ESWT group had significantly higher leak point pressures compared with untreated rats. Increased urethral and vaginal smooth and striated muscle content and increased thickness of the vaginal wall were noted in the SUI-Li-ESWT group. The SUI group had significantly decreased neuronal nitric oxide /tyrosine hydroxylase positive nerves ratio in the smooth muscle layers of the urethra, while the SUI-Li-ESWT group had neuronal nitric oxide/tyrosine hydroxylase+ nerves ratio similar to that of the control group. The continuality of urothelial cell lining was also improved in the SUI-Li-ESWT group. In addition, there were significantly increased EdU-positive cells in the SUI-Li-ESWT group., Conclusion: Li-ESWT appears to increase smooth muscle content in the urethra and the vagina, increase the thickness of urethral wall, improve striated muscle content and neuromuscular junctions, restore the integrity of the urothelium, and increase the number of EdU-retaining progenitor cells in the urethral wall., (Copyright © 2020 Elsevier Inc. All rights reserved.) more...
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- 2020
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45. Development of Male External Urethral Sphincter and Tissue-Resident Stem/Progenitor Cells in Rats.
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Zhou F, Reed-Maldonado AB, Tan Y, Yuan H, Peng D, Banie L, Wang G, Hou J, Lin G, and Lue TF
- Subjects
- Adult Stem Cells physiology, Animals, Cell Self Renewal, Cells, Cultured, Male, Rats, Rats, Sprague-Dawley, Urethra physiology, Adult Stem Cells cytology, Muscle Development, Urethra cytology
- Abstract
Stress urinary incontinence (SUI) after prostate surgery is primarily caused by urethral sphincter damage. There are few effective therapeutic approaches for male SUI due to both insufficient study of the structure of the external urethral sphincter (EUS) and incomplete understanding of the resident EUS stem/progenitor cells. The goals of this study were to localize and to determine the distribution of tissue-resident stem/progenitor cells in the male EUS throughout EUS development and to understand the anatomic temporal patterns of the EUS. Newborn Sprague Dawley rats were intraperitoneally injected with the thymidine analogue, 5-ethynyl-2-deoxyuridine (EdU), and the EUS was harvested at five time points (1, 2, 3, 4, and 8 weeks postinjection). The tissue was then processed for EdU staining and immunofluorescence staining for stem cell markers Ki67 and proliferating cell nuclear antigen. We counted the EdU+ label-retaining cells (LRCs) at each time point and colocalized with each stem cell marker, also we isolated and cultured the cells in vitro. The results revealed that the number of EdU+ LRCs in each EUS cross-section decreased over time and that the LRCs were located immediately under the basal membrane of laminin, densely adherent to the muscle fibers. In addition, the thickness of the striated muscle layer developed much faster than the smooth muscle layer during EUS development. By 4 weeks, the structure of the EUS layers was well differentiated. The EUS resident stem/progenitor cells were isolated with MACS
® MicroBeads system, and myogenesis was confirmed. In this study, we defined both the time-course development of the EUS and the distribution of resident stem/progenitor cells. This information is crucial for forthcoming studies regarding male micturition and for development of novel therapeutic approaches for postoperative male SUI. more...- Published
- 2020
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46. Smooth Muscle Differentiation of Penile Stem/Progenitor Cells Induced by Microenergy Acoustic Pulses In Vitro.
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Peng D, Yuan H, Liu T, Wang T, Reed-Maldonado AB, Kang N, Banie L, Wang G, Tang Y, He L, Lin G, and Lue TF
- Subjects
- Animals, Endothelial Cells metabolism, Erectile Dysfunction therapy, Male, Muscle, Smooth, Penile Erection physiology, Penis, Rats, Rats, Sprague-Dawley, Acoustics, Extracorporeal Shockwave Therapy methods, Myocytes, Smooth Muscle metabolism, Stem Cells metabolism
- Abstract
Introduction: Modulating tissue-resident stem and progenitor cells with a non-invasive, mechanobiological intervention is an optimal approach for tissue regeneration. Stem cell antigen-1 (Sca-1) has been identified as a stem cell marker within many organs but never within the penis., Aim: To localize and isolate penile stem/progenitor cells (PSPCs) and to evaluate cellular differentiation after exposure to induction medium and microenergy acoustic pulse (MAP) therapy., Methods: Six male Sprague-Dawley rats were used to isolate PSPCs. Isolation was followed by stem cell characterization and differentiation assays. The PSPCs were then treated with MAP (0.033 mJ/mm
2 , 1 Hz) at various dosages (25, 50, 100, and 200 pulses) and for different durations (1, 2, 4, 6, or 8 hours) in vitro., Main Outcome Measure: The PSPCs (Sca-1-positive cells) were isolated using the magnetic-activated cell sorting system. PSPC cellular differentiation was assessed after induction with induction medium and with MAP in vitro. Wnt/β-catenin signaling was also assayed., Results: The PSPCs were successfully localized within the penile subtunic and perisinusoidal spaces, and they were successfully isolated using magnetic-activated cell sorting. The stemness of the cells was confirmed by stem cell marker characterization and by multiple differentiation into smooth muscle cells, endothelial cells, adipocytes, and neurons. MAP-induced PSPCs differentiated into smooth muscle cells by activating the Wnt/β-catenin signaling pathway in a time- and dosage-dependent manner., Clinical Implications: By modulating resident PSPCs, MAP may have utility in the treatment of erectile dysfunction (ED)., Strengths & Limitations: This study provides solid evidence in support of microenergy therapies, including both MAP and low-intensity extracorporeal shock wave therapy, for the treatment of ED. Additional studies are needed and should include additional stem cells markers. Furthermore, studies exploring the underling mechanisms for PSPC activation and differentiation are required., Conclusion: PSPCs were successfully identified, localized, and isolated. Additionally, MAP provoked PSPCs to differentiate into smooth muscle cells via the Wnt/β-catenin signaling pathway. As such, MAP provides a novel method for activating endogenous tissue-resident stem/progenitor cells and might facilitate stem cell regenerative therapy targeting ED. Peng D, Yuan H, Liu T, et al. Smooth Muscle Differentiation of Penile Stem/Progenitor Cells Induced by Microenergy Acoustic Pulses In Vitro. J Sex Med 2019; 16:1874-1884., (Copyright © 2019 International Society for Sexual Medicine. Published by Elsevier Inc. All rights reserved.) more...- Published
- 2019
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47. The effects of microenergy acoustic pulses on animal model of obesity-associated stress urinary incontinence. Part 2: In situ activation of pelvic floor and urethral striated muscle progenitor cells.
- Author
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Kang N, Peng D, Wang B, Ruan Y, Zhou J, Reed-Maldonado AB, Banie L, Wang G, Xing N, Tang Y, Lin G, and Lue TF
- Subjects
- Acoustic Stimulation, Acoustics, Animals, Antigens, CD metabolism, Cell Proliferation, Deoxyuridine, Disease Models, Animal, Female, Flow Cytometry, Focal Adhesion Protein-Tyrosine Kinases metabolism, Integrin alpha Chains metabolism, Muscle Contraction physiology, Muscle, Skeletal cytology, Muscle, Striated cytology, Muscle, Striated physiology, Myoblasts physiology, Obesity complications, Paired Box Transcription Factors, Rats, Rats, Zucker, Regeneration, Stem Cells, Urethra cytology, Urinary Incontinence, Stress etiology, Wnt Signaling Pathway, Muscle, Skeletal physiology, Obesity physiopathology, Pelvic Floor physiopathology, Satellite Cells, Skeletal Muscle physiology, Urethra physiopathology, Urinary Incontinence, Stress physiopathology
- Abstract
Aim: To investigate the possibility and mechanism of microenergy acoustic pulses (MAP) for activating tissue resident stem/progenitor cells within pelvic and urethral muscle and possible mechanism., Methods: The female Zucker Lean and Zucker Fatty rats were randomly divided into four groups: ZL control, ZLMAP, ZF control, and ZFMAP. MAP was applied at 0.033 mJ/mm
2 , 3 Hz for 500 pulses, and the urethra and pelvic floor muscles of each rat was then harvested for cell isolation and flow cytometry assay. Freshly isolated cells were analyzed by flow cytometry for Pax-7, Int-7α, H3P, and EdU expression. Meanwhile, pelvic floor muscle-derived stem cells (MDSCs) were harvested through magnetic-activated cell sorting, MAP was then applied to MDSCs to assess the mechanism of stem cell activation., Results: Obesity reduced EdU-label-retaining cells and satellite cells in both pelvic floor muscle and urethra, while MAP activated those cells and enhanced cell proliferation, which promoted regeneration of striated muscle cells of the pelvic floor and urethral sphincter. Activation of focal adhesion kinase (FAK)/AMP-activated protein kinase (AMPK) /Wnt/β-catenin signaling pathways by MAP is the potential mechanism., Conclusions: MAP treatment activated tissue resident stem cells within pelvic floor and urethral muscle in situ via activating FAK-AMPK and Wnt/β-catenin signaling pathway., (© 2019 Wiley Periodicals, Inc.) more...- Published
- 2019
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48. The effects of microenergy acoustic pulses on an animal model of obesity-associated stress urinary incontinence. Part 1: Functional and histologic studies.
- Author
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Wang B, Ruan Y, Zhou T, Wang L, Li H, Peng D, Reed-Maldonado AB, Sanford MT, Lee YC, Zhou J, Wang HS, Banie L, Wang G, Liu J, Lin G, and Lue TF
- Subjects
- Acoustics, Animals, Disease Models, Animal, Female, Muscle Contraction physiology, Muscle, Striated physiopathology, Obesity complications, Rats, Rats, Zucker, Urethra physiopathology, Urinary Incontinence, Stress etiology, Acoustic Stimulation, Muscle, Skeletal physiopathology, Obesity physiopathology, Pelvic Floor physiopathology, Urinary Bladder physiopathology, Urinary Incontinence, Stress physiopathology
- Abstract
Aim: Obesity is a strong independent risk factor for urinary incontinence. Effective therapeutic approaches for obesity-associated stress urinary incontinence (OA-SUI) are lacking as the mechanisms remain unclear. The aim of our study is to explore the impacts of microenergy acoustic pulse (MAP) therapy on urethral and pelvic floor muscle structure and function in female lean and fatty rats., Methods: A total 24 Zucker fatty (ZF) and 24 Zucker lean (ZL) female 24-week-old rats were grouped into four groups: ZL control, ZLMAP, ZF control, and ZFMAP. For MAP treatment, 500 pulses were delivered at an energy level of 0.033 mJ/mm
2 and a frequency of 3 Hz and were applied twice a week for 4 weeks. After a 1-week washout, all rats underwent conscious cystometry and leak-point pressure (LPP) measurements followed by ex vivo organ-bath assay and histological study., Results: ZF rats had lower LPP as compared to ZL rats, and MAP treatment significantly improved LPP in ZF rats (P < .05). Impaired muscle contractile activity (MCA) in organ-bath study was noted in ZF rats. MAP treatment significantly increased MCA in ZF rats (P < .05) and also increased the thickness of the striated muscle layer and the number of neuromuscular junctions (NMJs). In situ, MAP activated muscle satellite cells significantly (P < .05)., Conclusions: Obesity impairs the function of both the urethral sphincter and the pelvic floor and leads to atrophy and distortion of the striated muscle in obese female rats. These issues contribute to OA-SUI. MAP improves continence by stimulating muscle regeneration and nerve innervation as well as by activating satellite cells., (© 2019 Wiley Periodicals, Inc.) more...- Published
- 2019
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49. Microenergy acoustic pulses induced myogenesis of urethral striated muscle stem/progenitor cells.
- Author
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Cui K, Kang N, Banie L, Zhou T, Liu T, Wang B, Ruan Y, Peng D, Wang HS, Wang T, Wang G, Reed-Maldonado AB, Chen Z, Lin G, and Lue TF
- Abstract
Background: Stress urinary incontinence (SUI) is a common disorder with high prevalence in women across their life span, but there are no non-surgical curative options for the condition. Stem cell-based therapy, especially endogenous stem cell therapy may be a potential treatment method for SUI. The aims of this study are to identify, isolate, and assay the function of urethral striated muscle derived stem/progenitor cells (uMDSCs) and to assess uMDSC response to microenergy acoustic pulses (MAP)., Methods: Urethral striated muscle was identified utilizing 3D imaging of solvent organs (3DISCO) and immunofluorescence (IF). uMDSCs were isolated and purified from Zucker Lean (ZL) (ZUC-LEAN) (ZUC-Leprfa 186) rats, with magnetic-activated cell sorting (MACS) and pre-plating methods. The stemness and differentiation potential of the uMDSCs were measured by cell proliferation, EdU, flow cytometry, IF, and Western blot., Results: Comparison of the cell proliferation assays between MACS and pre-plating reveals the advantage of MACS over pre-plating. In addition, the study reveals that uMDSCs form myotubes when treated with MAP., Conclusions: The uMDSCs within female rat urethral striated muscle could be a therapeutic target of MAP in managing SUI., Competing Interests: Conflicts of Interest: Tom F. Lue is consultant of AWCT, Inc. The other authors have no conflicts of interest to declare., (2019 Translational Andrology and Urology. All rights reserved.) more...
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- 2019
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50. Delayed Low-Intensity Extracorporeal Shock Wave Therapy Ameliorates Impaired Penile Hemodynamics in Rats Subjected to Pelvic Neurovascular Injury.
- Author
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Wang HS, Ruan Y, Banie L, Cui K, Kang N, Peng D, Liu T, Wang T, Wang B, Wang G, Shindel AW, Lin G, and Lue TF
- Subjects
- Animals, Disease Models, Animal, Erectile Dysfunction etiology, Hemodynamics, Male, Nerve Regeneration, Nitric Oxide Synthase Type I metabolism, Rats, Rats, Sprague-Dawley, Schwann Cells metabolism, Trauma, Nervous System complications, Erectile Dysfunction therapy, Extracorporeal Shockwave Therapy methods, Penile Erection physiology, Penis blood supply
- Abstract
Background: Erectile dysfunction (ED) caused by pelvic neurovascular injury (PNVI) is often refractory to treatment. In many cases, erectogenic therapy is administered in a delayed fashion., Aim: To evaluate penile hemodynamic effects and histologic changes associated with delayed low-intensity extracorporeal shock wave therapy (Li-ESWT) after PNVI ED in a rat model. We visualized images using immunofluorescence and 3-dimensional imaging of solvent-cleared organs (3DISCO), a novel imaging technique., Methods: A total of 32 Sprague-Dawley male rats aged 12 weeks were divided equally into 4 groups: sham surgery as normal controls (NC), PNVI controls (PC), PNVI with very-low-energy Li-ESWT (PVL), and PNVI with low-energy Li-ESWT (PL). Bilateral cavernous nerve crush and internal pudendal bundle ligation were performed in the 3 PNVI groups. Li-ESWT was administered twice a week for 4 weeks in the PL and PVL groups starting at 4 weeks after PNVI., Outcomes: Intracavernous pressure (ICP) studies (normalized to mean arterial pressure [MAP]) were conducted in all subject animals. After testing, tissue was harvested for immunofluorescence staining and 3DISCO analysis., Results: Mean ICP/MAP was lower in PC animals compared with NC animals (0.37 ± 0.03 vs 0.91 ± 0.03, respectively; P = .001). The ICP/MAP ratio was significantly higher in PVL and PL animals (0.66 ± 0.07 and 0.82 ± 0.05, respectively) compared with PC animals (P = .002 and .001, respectively). Detailed microstructures and trajectories of nerves and vessels were identified with immunofluorescence and 3DISCO. The PC group had lower density of nerves, axons, neuronal nitric oxide synthase-positive nerves, and Schwann cells in the dorsal penis. Animals in the PL group had significantly higher expression of all of these markers compared with PC animals., Clinical Implications: Li-EWST may have utility in the management of severe ED related to PNVI from severe pelvic injury or radical pelvic surgeries, even when administered in a delayed fashion., Strength & Limitations: This study of a severe ED phenotype involved treatment administered in a delayed fashion, which is more consistent with how therapy likely would be delivered in a real-world clinical context. Moreover, because the treatment commenced at 4 weeks after injury, when nerve and tissue atrophy have already occurred, the results imply that Li-ESWT can be used for regenerative therapy. Additional studies on dose optimization and treatment interval are needed to inform the design of human clinical trials., Conclusion: Li-ESWT ameliorates the negative functional and histologic effects of severe pelvic neurovascular injury in a rat model system. 3DISCO provides high-resolution images of neuroanatomy and neural regeneration. Wang HS, Ruan Y, Banie L, et al. Delayed Low-Intensity Extracorporeal Shock Wave Therapy Ameliorates Impaired Penile Hemodynamics in Rats Subjected to Pelvic Neurovascular Injury. J Sex Med 2019;16:17-26., (Copyright © 2018 International Society for Sexual Medicine. Published by Elsevier Inc. All rights reserved.) more...
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- 2019
- Full Text
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