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46 results on '"Banerjee, Pallab"'

45. Incongruity of Imaging Using Fluorescent 2-DG Conjugates Compared to F-FDG in Preclinical Cancer Models.

Author

Tseng, Jen-Chieh, Wang, Yuchuan, Banerjee, Pallab, and Kung, Andrew

Subjects
IMAGING of cancer, MEDICAL imaging systems, NEAR infrared radiation, MOLECULAR biology, RESEARCH
Abstract

Purpose: We compared the use of near-infrared conjugates of 2-deoxyglucose (NIR 2-DG) to 2-deoxy-2-[F]fluoro- d-glucose (F-FDG) for the purposes of imaging tumors, as well as response to therapy. Procedures: Uptake of both F-FDG and NIR 2-DG within gastrointestinal stromal tumor xenografts were imaged before and after nilotinib treatment. Confocal microscopy was performed to determine NIR 2-DG distribution in tumors. Results: Treatment with nilotinib resulted in a rapid reduction in F-FDG uptake and reduced tumor cell viability which was predictive of long-term antitumor efficacy. In contrast, optical imaging with NIR 2-DG probes was unable to differentiate control from niltonib-treated animals, and microscopic analysis revealed no change in probe distribution as a result of treatment. Conclusions: These results suggest that conjugation of large bulky fluorophores to 2-DG disrupts the facilitated transport and retention of these probes in cells. Therefore, optical imaging of NIR 2-DG probes cannot substitute for F-FDG positron emission tomography imaging as a biomarker of tumor cell viability and metabolism. [ABSTRACT FROM AUTHOR]

Published
2012
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46. Linear polyethyleneimine grafted to a hyperbranched poly(ethylene glycol)-like core: a copolymer for gene delivery.

Author

Banerjee P, Weissleder R, and Bogdanov A Jr

Subjects
Animals, COS Cells, Cell Death drug effects, Chlorocebus aethiops, DNA administration & dosage, DNA chemistry, DNA metabolism, Deoxyribonuclease I metabolism, Nuclease Protection Assays, Plasmids genetics, Polyethylene Glycols administration & dosage, Polyethyleneimine administration & dosage, Polymers administration & dosage, Polymers chemistry, Polyethylene Glycols chemistry, Polyethyleneimine chemistry, Polymers chemical synthesis, Transfection methods
Abstract

A block copolymer of a hyperbranched poly(ethylene glycol)-like core and linear polyethylenimine (HBP) was synthesized by a facile synthetic route that included (1) a single-step cationic copolymerization of diepoxy and polyhydroxyl monomers, (2) derivatization of hydroxyl groups of the core HBPEG copolymer with either tosyl or chloromethylbenzoyl chlorides resulting in a corresponding macroinitiator, and (3) synthesis of HBPEG-block-poly(alkyl oxazolines). HBPEG-block-linear polyethyleneimine (HBP) was obtained by hydrolysis of HBPEG-block-poly(alkyl oxazolines). Linear PEI-bearing hyperbranched polycations (HBP) had lower inherent toxicity in cell culture than PEG-grafted linear polyethyleneimines (PEGLPEI). PEGLPEI formed a complex with DNA with an average diameter of 250 nm. The complexes were loosely condensed and formed aggregates and precipitates during storage. By contrast, hyperbranched polycations (HBP) formed approximately 50 nm nanocomplexes with DNA that were stable for several weeks and showed resistance to DNAse I-mediated degradation. The 'inverted' block copolymers showed several orders of magnitude higher transfection efficiency than PEGLPEI in vitro. Because of the biocompatibility and higher transfection efficiency, the 'inverted' block copolymer merits further investigation as a gene carrier.

Published
2006
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