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117 results on '"Ballout, Farah"'

1. Data from Targeting SMAD3 Improves Response to Oxaliplatin in Esophageal Adenocarcinoma Models by Impeding DNA Repair

Author

Ballout, Farah, primary, Lu, Heng, primary, Bhat, Nadeem, primary, Chen, Lei, primary, Peng, Dunfa, primary, Chen, Zheng, primary, Chen, Steven, primary, Sun, Xiaodian, primary, Giordano, Silvia, primary, Corso, Simona, primary, Zaika, Alexander, primary, McDonald, Oliver, primary, Livingstone, Alan S., primary, and El-Rifai, Wael, primary

Published
2024
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2. Novel 2‑(5-Imino‑5H‑isoquinolones[3,4‑b]quinoxalin-7-ylmethyl)-benzonitrile (DIQ3) and Other Related Derivatives Targeting Colon Cancer Cells: Syntheses and in Vitro Models

Author

Monzer, Alissar, Jabotian, Nayri, Ballout, Farah, Zhu, Jie S, Kurth, Mark J, Haddadin, Makhluf J, and Gali-Muhtasib, Hala

Subjects
Cancer, Stem Cell Research, Digestive Diseases, Colo-Rectal Cancer, Development of treatments and therapeutic interventions, 2.1 Biological and endogenous factors, Aetiology, 5.1 Pharmaceuticals, Chemical Engineering, Materials Engineering
Abstract

Chemotherapy has been shown to be effective in reducing the progression and development of cancer in metastatic patients. However, drug selectivity is still a major issue for most chemotherapeutics. In this study, we synthesized four novel heterocyclic compounds having similarity in structure with quinone systems whereby nitrogen atoms replace the oxygen atoms. The anticancer activity of these compounds (DIQ3-6) was tested against HCT116 human colon cancer cells. We showed that all four heterocycles caused significant reduction in colon cancer cell viability at doses as low as 4 μM, a concentration that was not cytotoxic to normal human FHs74Int intestinal cell lines. Interestingly, these heterocycles inhibited colon sphere formation in 3D cultures at first generation (G1), mainly because of inhibition of proliferation as evidenced by Ki67 staining. Thus, DIQ3 causes sufficient eradication of the self-renewal ability of the highly resistant cancer stem cells. This study represents the first documentation of the activity of these novel heterocyclic compounds, particularly compound DIQ3, and their potential therapeutic use in targeting colon cancer self-renewal capacity. Our findings provide the basis for proposing these nontoxic and stable compounds for additional testing against cancer.

Published
2019

4. Targeting SMAD3 Improves Response to Oxaliplatin in Esophageal Adenocarcinoma Models by Impeding DNA Repair

Author

Ballout, Farah, primary, Lu, Heng, additional, Bhat, Nadeem, additional, Chen, Lei, additional, Peng, Dunfa, additional, Chen, Zheng, additional, Chen, Steven, additional, Sun, Xiaodian, additional, Giordano, Silvia, additional, Corso, Simona, additional, Zaika, Alexander, additional, McDonald, Oliver, additional, Livingstone, Alan S., additional, and El-Rifai, Wael, additional

Published
2024
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11. Reflux conditions induce E-cadherin cleavage and EMT via APE1 redox function in oesophageal adenocarcinoma.

Author

Heng Lu, Long Long Cao, Ballout, Farah, Belkhiri, Abbes, DunFa Peng, Lei Chen, Zheng Chen, Soutto, Mohammed, Wang, Timothy C., Jianwen Que, Giordano, Silvia, Washington, Mary Kay, Chen, Steven, McDonald, Oliver Gene, Zaika, Alexander, and El-Rifai, Wael

Subjects
CADHERINS, MOLECULAR biology, GENE expression, NATURAL history, HEARTBURN, ADENOCARCINOMA, BARRETT'S esophagus, ESOPHAGEAL cancer
Published
2024
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16. Reflux conditions induce E-cadherin cleavage and EMT via APE1 redox function in oesophageal adenocarcinoma

Author

Lu, Heng, Cao, Long Long, Ballout, Farah, Belkhiri, Abbes, Peng, DunFa, Chen, Lei, Chen, Zheng, Soutto, Mohammed, Wang, Timothy C, Que, Jianwen, Giordano, Silvia, Washington, Mary Kay, Chen, Steven, McDonald, Oliver Gene, Zaika, Alexander, and El-Rifai, Wael

Abstract

ObjectiveChronic gastro-oesophageal reflux disease, where acidic bile salts (ABS) reflux into the oesophagus, is the leading risk factor for oesophageal adenocarcinoma (EAC). We investigated the role of ABS in promoting epithelial-mesenchymal transition (EMT) in EAC.DesignRNA sequencing data and public databases were analysed for the EMT pathway enrichment and patients’ relapse-free survival. Cell models, pL2-IL1β transgenic mice, deidentified EAC patients’ derived xenografts (PDXs) and tissues were used to investigate EMT in EAC.ResultsAnalysis of public databases and RNA-sequencing data demonstrated significant enrichment and activation of EMT signalling in EAC. ABS induced multiple characteristics of the EMT process, such as downregulation of E-cadherin, upregulation of vimentin and activation of ß-catenin signalling and EMT-transcription factors. These were associated with morphological changes and enhancement of cell migration and invasion capabilities. Mechanistically, ABS induced E-cadherin cleavage via an MMP14-dependent proteolytic cascade. Apurinic/apyrimidinic endonuclease (APE1), also known as redox factor 1, is an essential multifunctional protein. APE1 silencing, or its redox-specific inhibitor (E3330), downregulated MMP14 and abrogated the ABS-induced EMT. APE1 and MMP14 coexpression levels were inversely correlated with E-cadherin expression in human EAC tissues and the squamocolumnar junctions of the L2-IL1ß transgenic mouse model of EAC. EAC patients with APE1highand EMThighsignatures had worse relapse-free survival than those with low levels. In addition, treatment of PDXs with E3330 restrained EMT characteristics and suppressed tumour invasion.ConclusionReflux conditions promote EMT via APE1 redox-dependent E-cadherin cleavage. APE1-redox function inhibitors can have a therapeutic role in EAC.

Published
2024
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17. Combination of 5-fluorouracil and thymoquinone targets stem cell gene signature in colorectal cancer cells

Author

Ndreshkjana, Benardina, Çapci, Aysun, Klein, Volker, Chanvorachote, Pithi, Muenzner, Julienne K., Huebner, Kerstin, Steinmann, Sara, Erlenbach-Wuensch, Katharina, Geppert, Carol I., Agaimy, Abbas, Ballout, Farah, El-Baba, Chirine, Gali-Muhtasib, Hala, Roehe, Adriana Vial, Hartmann, Arndt, Tsogoeva, Svetlana B., and Schneider-Stock, Regine

Published
2019
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20. Novel therapeutic diiminoquinone exhibits anticancer effects on human colorectal cancer cells in two-dimensional and three-dimensional in vitro models

Author

Monzer, Alissar, primary, Wakimian, Kevork, additional, Ballout, Farah, additional, Al Bitar, Samar, additional, Yehya, Amani, additional, Kanso, Mariam, additional, Saheb, Nour, additional, Tawil, Ayman, additional, Doughan, Samer, additional, Hussein, Maher, additional, Mukherji, Deborah, additional, Faraj, Walid, additional, Gali-Muhtasib, Hala, additional, and Abou-Kheir, Wassim, additional

Published
2022
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21. Activation of NOTCH signaling via DLL1 is mediated by APE1-redox-dependent NF-κB activation in oesophageal adenocarcinoma

Author

Chen, Lei, primary, Lu, Heng, additional, Peng, Dunfa, additional, Cao, Long Long, additional, Ballout, Farah, additional, Srirmajayam, Kannappan, additional, Chen, Zheng, additional, Bhat, Ajaz, additional, Wang, Timothy C, additional, Capobianco, Anthony, additional, Que, Jianwen, additional, McDonald, Oliver Gene, additional, Zaika, Alexander, additional, Zhang, Shutian, additional, and El-Rifai, Wael, additional

Published
2022
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27. Activation of NOTCH signaling via DLL1 is mediated by APE1-redox-dependent NF-κB activation in oesophageal adenocarcinoma.

Author

Lei Chen, Heng Lu, Dunfa Peng, Long Long Cao, Ballout, Farah, Srirmajayam, Kannappan, Zheng Chen, Bhat, Ajaz, Wang, Timothy C., Capobianco, Anthony, Que, Jianwen, McDonald, Oliver Gene, Zaika, Alexander, Shutian Zhang, and El-Rifai, Wael

Subjects
DNA ligases, CARCINOGENS, TRANSCRIPTION factors, ADENOCARCINOMA, SMALL molecules, ESOPHAGEAL cancer, BARRETT'S esophagus, HEARTBURN
Published
2023
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30. Establishment and characterization of prostate organoids from treatment‑naïve patients with prostate cancer

Author

Cheaito, Katia, primary, Bahmad, Hisham, additional, Hadadeh, Ola, additional, Msheik, Hiba, additional, Monzer, Alissar, additional, Ballout, Farah, additional, Dagher, Christelle, additional, Telvizian, Talar, additional, Saheb, Nour, additional, Tawil, Ayman, additional, El‑Sabban, Marwan, additional, El‑Hajj, Albert, additional, Mukherji, Deborah, additional, Al‑Sayegh, Mohamed, additional, and Abou‑kheir, Wassim, additional

Published
2021
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31. Activation of NOTCH signaling via DLL1 is mediated by APE1-redox-dependent NF-κB activation in oesophageal adenocarcinoma

Author

Chen, Lei, Lu, Heng, Peng, Dunfa, Cao, Long Long, Ballout, Farah, Srirmajayam, Kannappan, Chen, Zheng, Bhat, Ajaz, Wang, Timothy C, Capobianco, Anthony, Que, Jianwen, McDonald, Oliver Gene, Zaika, Alexander, Zhang, Shutian, and El-Rifai, Wael

Abstract

ObjectiveOesophageal adenocarcinoma (EAC) arises in the setting of Barrett’s oesophagus, an intestinal metaplastic precursor lesion that can develop in patients with chronic GERD. Here, we investigated the role of acidic bile salts, the mimicry of reflux, in activation of NOTCH signaling in EAC.DesignThis study used public databases, EAC cell line models, L2-IL1β transgenic mouse model and human EAC tissue samples to identify mechanisms of NOTCH activation under reflux conditions.ResultsAnalysis of public databases demonstrated significant upregulation of NOTCH signaling components in EAC. In vitro studies demonstrated nuclear accumulation of active NOTCH1 cleaved fragment (NOTCH intracellular domain) and upregulation of NOTCH targets in EAC cells in response to reflux conditions. Additional investigations identified DLL1 as the predominant ligand contributing to NOTCH1 activation under reflux conditions. We discovered a novel crosstalk between APE1 redox function, reflux-induced inflammation and DLL1 upregulation where NF-κB can directly bind to and induce the expression of DLL1. The APE1 redox function was crucial for activation of the APE1-NF-κB-NOTCH axis and promoting cancer cell stem-like properties in response to reflux conditions. Overexpression of APE1 and DLL1 was detected in gastro-oesophageal junctions of the L2-IL1ß transgenic mouse model and human EAC tissue microarrays. DLL1 high levels were associated with poor overall survival in patients with EAC.ConclusionThese findings underscore a unique mechanism that links redox balance, inflammation and embryonic development (NOTCH) into a common pro-tumorigenic pathway that is intrinsic to EAC cells.

Published
2023
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40. Tideglusib attenuates growth of neuroblastoma cancer stem/progenitor cells in vitro and in vivo by specifically targeting GSK-3β

Author

Bahmad, Hisham F., primary, Chalhoub, Reda M., additional, Harati, Hayat, additional, Bou-Gharios, Jolie, additional, Assi, Sahar, additional, Ballout, Farah, additional, Monzer, Alissar, additional, Msheik, Hiba, additional, Araji, Tarek, additional, Elajami, Mohamad K., additional, Ghanem, Paola, additional, Chamaa, Farah, additional, Kadara, Humam, additional, Abou-Antoun, Tamara, additional, Daoud, Georges, additional, Fares, Youssef, additional, and Abou-Kheir, Wassim, additional

Published
2020
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41. Abstract 2802: Establishing and optimizing treatment-naïve prostate cancer patient-derived organoids culture: Implications for personalized medicine

Author

Cheaito, Katia, primary, Bahmad, Hisham, additional, Monzer, Alissar, additional, Ballout, Farah, additional, Msheik, HIba, additional, Hadadeh, Ola, additional, Dagher, Christelle, additional, Saheb, Nour, additional, Tawil, Ayman, additional, El-Sabban, Marwan, additional, El-Hajj, Albert, additional, Mukherji, Deborah, additional, and Abou-Kheir, Wassim G., additional

Published
2020
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46. Specific Inhibition of GSK-3β by Tideglusib: Potential Therapeutic Target for Neuroblastoma Cancer Stem Cells

Author

Bahmad, Hisham F., primary, Chalhoub, Reda M., additional, Harati, Hayat, additional, Bou-Gharios, Jolie, additional, Ballout, Farah, additional, Monzer, Alissar, additional, Msheik, Hiba, additional, Assi, Sahar, additional, Araji, Tarek, additional, Elajami, Mohamad K., additional, Ghanem, Paola, additional, Chamaa, Farah, additional, Kadara, Humam, additional, Abou-Antoun, Tamara, additional, Daoud, Georges, additional, Fares, Youssef, additional, and Abou-Kheir, Wassim, additional

Published
2020
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50. Establishment and characterization of prostate organoids from treatment-naïve patients with prostate cancer.

Author

Cheaito, Katia, Bahmad, Hisham F., Hadadeh, Ola, Msheik, Hiba, Monzer, Alissar, Ballout, Farah, Dagher, Christelle, Telvizian, Talar, Saheb, Nour, Tawil, Ayman, El-Sabban, Marwan, El-Hajj, Albert, Mukherji, Deborah, Al-Sayegh, Mohamed, and Abou-Kheir, Wassim

Subjects
PROSTATE cancer patients, PROSTATE cancer, ORGANOIDS, RADICAL prostatectomy, PROSTATE, CELL suspensions
Abstract

Three-dimensional (3D) organoid culture systems are emerging as potential reliable tools to investigate basic developmental processes of human disease, especially cancer. The present study used established and modified culture conditions to report successful generation and characterization of patient-derived organoids from fresh primary tissue specimens of patients with treatment-naïve prostate cancer (PCa). Fresh tissue specimens were collected, digested enzymatically and the resulting cell suspensions were plated in a 3D environment using Matrigel as an extracellular matrix. Previously established 12-factor medium for organoid culturing was modified to create a minimal 5-factor medium. Organoids and corresponding tissue specimens were characterized using transcriptomic analysis, immunofluorescent analysis, and immunohistochemistry. Furthermore, patient-derived organoids were used to assess the drug response. Treatment-naïve patient-derived PCa organoids were obtained from fresh radical prostatectomy specimens. These PCa organoids mimicked the heterogeneity of corresponding parental tumor tissue. Histopathological analysis demonstrated similar tissue architecture and cellular morphology, as well as consistent immunohistochemical marker expression. Also, the results confirmed the potential of organoids as an in vitro model to assess potential personalized treatment responses as there was a differential drug response between different patient samples. In conclusion, the present study investigated patient-derived organoids from a cohort of treatment-naïve patients. Derived organoids mimicked the histological features and prostate lineage profiles of their corresponding parental tissue and may present a potential model to predict patient-specific treatment response in a pre-clinical setting. [ABSTRACT FROM AUTHOR]

Published
2022

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