Search

The overwhelming majority of studies examining environmental change deliver treatments abruptly, although, in fact, many important changes are gradual. One example of a gradually increasing environmental stressor is heavy metal contamination. Essential heavy metals, such as copper, play an important role within cells of living organisms but are toxic at higher concentrations. In our study, we focus on the effects of copper pollution on filamentous soil fungi, key players in terrestrial ecosystem functioning. We hypothesize that fungi exposed to gradually increasing copper concentrations have higher chances for physiological acclimation and will maintain biomass production and accumulate less copper, compared to fungi abruptly exposed to the highest copper concentration. To test this hypothesis, we conducted an experiment with 17 fungal isolates exposed to gradual and abrupt copper addition. Contrary to our hypothesis, we find diverse idiosyncratic responses, such that for many fungi gradually increasing copper concentrations have more severe effects (stronger growth inhibition and higher copper accumulation) than an abrupt increase. While a number of environmental change studies have accumulated evidence based on the magnitude of changes, the results of our study imply that the rate of change can be an important factor to consider in future studies in ecology, environmental science, and environmental management.

Anthropogenic disturbances pose a multitude of novel challenges to ecosystems. While many experiments have tested effects using abrupt treatment applications, most environmental changes in fact are gradual. Since ecosystem responses might be highly dependent on the temporal nature of stressors, it is crucial to differentiate the effects of abrupt vs gradual treatment application. Antifungal agents, which are widely used in disease control both for humans and in agriculture, are becoming a new class of environmental contaminants. In this study, we examined the effect of a sub-lethal application of one antifungal agent, amphotericin B. We applied different rates of delivery, e.g. gradual and abrupt, and monitored biomass and sporulation of the model fungus Neurospora crassa in a batch culture. Our results demonstrate that: (i) the effect size difference between abrupt and gradual treatments is fungal growth stage dependent and (ii) the gradual treatment clearly had a higher sporulation level compared with all types of abrupt treatments. Our findings highlight the importance of considering the rate of change in environmental change research and point to a new research direction for future global change studies. Furthermore, our results also have important implications for avoiding treatment-induced spore production in agriculture and medical practise.

The overwhelming majority of studies examining environmental change deliver treatments abruptly, although, in fact, many important changes are gradual. One example of a gradually increasing environmental stressor is heavy metal contamination. Essential heavy metals, such as copper, play an important role within cells of living organisms but are toxic at higher concentrations. In our study, we focus on the effects of copper pollution on filamentous soil fungi, key players in terrestrial ecosystem functioning. We hypothesize that fungi exposed to gradually increasing copper concentrations have higher chances for physiological acclimation and will maintain biomass production and accumulate less copper, compared to fungi abruptly exposed to the highest copper concentration. To test this hypothesis, we conducted an experiment with 17 fungal isolates exposed to gradual and abrupt copper addition. Contrary to our hypothesis, we find diverse idiosyncratic responses, such that for many fungi gradually increasing copper concentrations have more severe effects (stronger growth inhibition and higher copper accumulation) than an abrupt increase. While a number of environmental change studies have accumulated evidence based on the magnitude of changes, the results of our study imply that the rate of change can be an important factor to consider in future studies in ecology, environmental science, and environmental management.

Climate change can trigger shifts in community structure and may therefore pose a severe threat to soil microbial communities, especially in high northern latitudes such as the Arctic. Arctic soils are covered by snow and ice throughout most of the year. This insulation shields them from high temperature variability and low surface temperatures. If this protective layer thaws, these soils are predicted to warm up at 1.5x to 4x the rate of other terrestrial biomes. In this study, we sampled arctic soils from sites with different elevations in Alaska, incubated them for 5 months with a simulated, gradual or abrupt temperature increase of +5 °C, and compared bacterial and fungal community compositions after the incubation. We hypothesized that the microbial communities would not significantly change with a gradual temperature treatment, whereas an abrupt temperature increase would decrease microbial diversity and shift community composition. The only differences in community composition that we observed were, however, related to the two elevations. The abrupt and gradual temperature increase treatments did not change the microbial community composition as compared to the control indicating resistance of the microbial community to changes in temperature. This points to the potential importance of microbial dormancy and resting stages in the formation of a “buffer” against elevated temperatures. Microbial resting stages might heavily contribute to microbial biomass and thus drive the responsiveness of arctic ecosystems to climate change.