Search

In the present investigation, formulate the new ecofriendly, low-cost commercial fertilizer for pilot-scale cultivation of a green microalgae, Tetradesmus obliquus RDS01, using bubble column photo bioreactor. Initially, we optimized the four different fertilizers, viz., urea—500 mg L−1, di-ammonium phosphate (DAP)—350 mg L−1, potassium—280 mg L−1, and sodium bicarbonate—165 mgL−1 using response surface methodology (RSM). The Tetradesmus obliquus RDS01 microalga was produced 350.25 mg g−1 of lipid, 240.35 mg g−1 of carbohydrate, 17.50 g L−1 of biomass, 98.52% of CO2 utilization efficiency, 325 U mL−1 of acetyl CoA carboxylase enzyme, 5.73 mL g−1 of biodiesel, and 4.25 mL g−1 of bioethanol obtained in pilot-scale cultivation in optimized commercial fertilizer medium. The Acetyl CoA carboxylase enzyme protein molecular weight was observed at 64KDa on 15% SDS-PAGE. The 3D structure homology modeling of Acetyl CoA carboxylase enzyme protein structure was predicted and protein encodes with 498 amino acid residues. The Acetyl CoA carboxylase specific genes (aCCD) were 1230 bp isolated. The Tetradesmus obliquus RDS01 microalga–produced biodiesel was subjected to gas chromatograph mass spectrometer (GCMS) analysis and identified the 50 biodiesel compounds. The major biodiesel compounds, viz., Cis-8-methylicosanoate methyl ester (28.36%). Eicosanoic acid, methyl ester (12.36%), hexadecanoic acid methyl ester (13.15%), 14-heptadecatrienoic acid methyl ester (3.25%), tridecanoic acid methyl ester (10.96%), tetradecanoate methyl ester (11.35%), pentadecanoic acid methyl ester (12.57%). The presences of functional groups were identified from the biodiesel such as amides, alcohols, carboxylic acids, alkenes, alkenyl, carbonyl, and ketones groups using FTIR. The bioethanol was confirmed in the peak range 3.52 min by HPLC. [ABSTRACT FROM AUTHOR]

A pilot-scale treatment method was used in the present study to test the biosorption of textile dye from textile effluent and carbon dioxide using Chlorella vulgaris RDS03. The textile dye effluent treatment achieved that textile dye biosorption capacity (qmax) rate of 98.84% on 15 days of treatment using Chlorella vulgaris RDS03. The Langmuir and Freundlich isotherm kinetics model indicated that the higher R2 value 0.98. The microalga Chlorella vulgaris RDS03 was captured-96.86% of CO2 analyzed by CO2 utilization and biofixation kinetics, 4.65 mgmL−1 of biomass, 189.26 mgg−1 of carbohydrate, 233.89 mgg−1 of lipid, 4.3 mLg−1 of bioethanol and 4.9 mLg−1 of biodiesel produced. We performed fatty acid methyl ester (FAME) profiling using gas chromatography-mass spectrometry (GCMS). We found 40 types of biodiesel compounds, specifically myristic acid, pentadecanoic acid, octadecanoic acid, palmitic acid, and oleic acid. The high-performance liquid chromatography (HPLC) validated and analyzed the produced bioethanol. Novelty of the Research • Unicellular microalga Chlorella vulgaris RDS03 was isolated from the freshwater region and investigated their biosorption efficiency against hazardous synthetic azo textile dyes. • Chlorella vulgaris RDS03 ability to biosorption 96.86% of environmental polluted carbon dioxide • Treated biomass was used to produce ecofriendly, unpolluted and green energy such as biofuels (biodiesel and bioethanol) [ABSTRACT FROM AUTHOR]

In present investigation carried out large-scale treatment of tannery effluent by the cultivation of microalgae, Neochloris aquatica RDS02. The tannery effluent treatment revealed that significant reduction heavy metals were chromium-3.59, lead-2.85, nickel-1.9, cadmium-10.68, zinc-4.49, copper-0.95 and cobalt-1.86 mg/L on 15th day of treatment using N. aquatica RDS02. The microalgal biosorption capacity qmax rate was Cr-88.66, Pb-75.87, Ni-87.61, Cd-60.44, Co-52.86, Zn-84.90 and Cu-54.39, and isotherm model emphasized that the higher R2 value 0.99 by Langmuir and Freundlich kinetics model. The microalga utilized highest CO2 (90%) analyzed by CO2 biofixation and utilization kinetics, biomass (3.9 mg/mL), lipid (210 mg mL−1), carbohydrate (102.75 mg mL−1), biodiesel (4.9 mL g−1) and bioethanol (4.1 mL g−1). The microalgal-lipid content was analyzed through Nile red staining. Gas chromatography mass spectrometric (GCMS) analysis confirmed that the presence of a biodiesel and major fatty acid methyl ester (FAME) profiling viz., tridecanoic acid methyl ester, pentadecanoic acid methyl ester, octadecanoic acid methyl ester, myristic acid methyl ester, palmitic acid methyl ester and oleic acid methyl ester. Fourier transform infrared (FTIR) analysis confirmed that the presence of a functional groups viz., phenols, alcohols, alkynes, carboxylic acids, ketones, carbonyl and ester groups. The bioethanol production was confirmed by high-performance liquid chromatography (HPLC) analyze. [ABSTRACT FROM AUTHOR]

In present investigation carried out large-scale treatment of tannery effluent by the cultivation of microalgae, Neochloris aquatica RDS02. The tannery effluent treatment revealed that significant reduction heavy metals were chromium-3.59, lead-2.85, nickel-1.9, cadmium-10.68, zinc-4.49, copper-0.95 and cobalt-1.86 mg/L on 15th day of treatment using N. aquatica RDS02. The microalgal biosorption capacity q max rate was Cr-88.66, Pb-75.87, Ni-87.61, Cd-60.44, Co-52.86, Zn-84.90 and Cu-54.39, and isotherm model emphasized that the higher R 2 value 0.99 by Langmuir and Freundlich kinetics model. The microalga utilized highest CO 2 (90%) analyzed by CO 2 biofixation and utilization kinetics, biomass (3.9 mg/mL), lipid (210 mg mL -1 ), carbohydrate (102.75 mg mL -1 ), biodiesel (4.9 mL g -1 ) and bioethanol (4.1 mL g -1 ). The microalgal-lipid content was analyzed through Nile red staining. Gas chromatography mass spectrometric (GCMS) analysis confirmed that the presence of a biodiesel and major fatty acid methyl ester (FAME) profiling viz ., tridecanoic acid methyl ester, pentadecanoic acid methyl ester, octadecanoic acid methyl ester, myristic acid methyl ester, palmitic acid methyl ester and oleic acid methyl ester. Fourier transform infrared (FTIR) analysis confirmed that the presence of a functional groups viz ., phenols, alcohols, alkynes, carboxylic acids, ketones, carbonyl and ester groups. The bioethanol production was confirmed by high-performance liquid chromatography (HPLC) analyze.