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1. A population-based study on seroprevalence and factors associated with SARS-CoV-2 infection in Córdoba, Argentina[Estudio de base poblacional de seroprevalencia y factores asociados a la infección por SARS-CoV-2 en Córdoba, Argentina]

Author

Aballay, Laura Rosana, Coquet, Julia Becaria, Scruzzi, Graciela Fabiana, Haluszka, Eugenia, Franchini, Carlos Germán, Carreño, Paula, Raboy, Elias, Román, María Dolores, Niclis, Camila, Balangero, Marcos, Altamirano, Natalia, Barbás, María Gabriela, and López, Laura

Subjects
R Medicina (General), RA0421 Salud pública. Higiene. Medicina Preventiva
Abstract

Los estudios seroepidemiológicos permiten conocer la distribución indirecta de las enfermedades, detectando marcadores séricos de inmunidad y demostrando infecciones no diagnosticadas en la población general. El objetivo fue estimar la seroprevalencia de anticuerpos contra el SARS-CoV-2, en Córdoba, Argentina, entre diciembre de 2020 y enero de 2021, e identificar factores asociados a la contagiosidad del virus. Se realizó un estudio observacional transversal, de base poblacional, con 3.225 individuos mayores de 2 años, residentes en Córdoba Capital, que fueron seleccionados mediante un diseño de muestreo aleatorio en múltiples etapas, proporcional a la distribución por género, franja etaria y nivel socioeconómico de la población de Córdoba. Las características clínicas, antropometría y comorbilidades se recogieron mediante entrevistas. Se realizó un test serológico cualitativo para la detección de anticuerpos IgG antinucleocápside para SARS-CoV-2 (ARCHITECT, Abbott). La seroprevalencia del SARS-CoV-2 se estimó en la población y por franja de edad, sexo, nivel socioeconómico y presencia de las patologías estudiadas. Las razones de prevalencia (RP) se estimaron usando un modelo de regresión log-binomial. La seropositividad para SARS-CoV-2 fue de 16,68% (IC95%: 15,41-18,01). Tener entre 2 y 18 años, residir en barrios con nivel socioeconómico bajo y la presencia de obesidad, aumentaron la oportunidad de seropositividad (RP = 1,50; IC95%: 1,10-2,04, RP = 1,91; IC95%: 1,34-2,67 y RP = 1,39; IC95%: 1,04-1,85). Los resultados indican que en Córdoba Capital existen atributos diferenciales que aumentan la posibilidad de ser seropositivo para SARS-CoV-2. Esto permite dirigir estrategias de vigilancia epidemiológica para reducir la propagación del virus., Fil: Aballay, Laura Rosana. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; Argentina, Fil: Aballay, Laura Rosana. Ministerio de Salud de la Provincia de Córdoba. Área de Epidemiología; Argentina, Fil: Coquet, Julia Becaria. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; Argentina, Fil: Scruzzi, Graciela Fabiana. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; Argentina, Fil: Scruzzi, Graciela Fabiana. Ministerio de Salud de la Provincia de Córdoba. Área de Epidemiología; Argentina, Fil: Scruzzi, Graciela Fabiana. Universidad Católica de Córdoba. Facultad de Ciencias de la Salud; Argentina, Fil: Haluszka, Eugenia. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; Argentina, Fil: Carreño, Paula. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; Argentina, Fil: Carreño, Paula. Ministerio de Salud de la Provincia de Córdoba. Área de Epidemiología; Argentina, Fil: Carreño, Paula. Universidad Católica de Córdoba. Facultad de Ciencias de la Salud; Argentina, Fil: Raboy, Elias. Ministerio de Salud de la Provincia de Córdoba. Área de Epidemiología; Argentina, Fil: Román, María Dolores. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; Argentina, Fil: Niclis, Camila. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas; Argentina, Fil: Balangero, Marcos. Ministerio de Salud de la Provincia de Córdoba. Área de Diagnóstico y Vigilancia; Argentina, Fil: Altamirano, Natalia. Laboratorio Central de la Provincia Córdoba; Argentina, Fil: Barbás, María Gabriela. Ministerio de Salud de la Provincia de Córdoba. Secretaría de Prevención y Promoción de la Salud; Argentina, Fil: López, Laura. Ministerio de Salud de la Provincia de Córdoba. Área de Epidemiología; Argentina

Published
2022

4. Estudio de base poblacional de seroprevalencia y factores asociados a la infección por SARS-CoV-2 en Córdoba, Argentina

Author

Aballay, Laura Rosana, primary, Coquet, Julia Becaria, additional, Scruzzi, Graciela Fabiana, additional, Haluszka, Eugenia, additional, Franchini, Germán, additional, Carreño, Paula, additional, Raboy, Elias, additional, Román, María Dolores, additional, Niclis, Camila, additional, Balangero, Marcos, additional, Altamirano, Natalia, additional, Barbás, María Gabriela, additional, and López, Laura, additional

Published
2022
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6. First Description of Seronegative HTLV-1 Carriers in Argentina

Author

Gallego, Sandra, primary, Frutos, María C., additional, Blanco, Sebastián, additional, Castro, Gonzalo, additional, Balangero, Marcos, additional, Elías Panigo, David, additional, Mangeaud, Arnaldo, additional, Remondegui, Carlos, additional, Santos Rocha, Anderson, additional, Melo Franco, Gabriela, additional, Lobato Martins, Marina, additional, Barbosa-Stancioli, Edel Figueiredo, additional, and Nates, Silvia, additional

Published
2020
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8. Estudio de base poblacional de seroprevalencia y factores asociados a la infección por SARS-CoV-2 en Córdoba, Argentina.

Author

Rosana Aballay, Laura, Becaria Coquet, Julia, Fabiana Scruzzi, Graciela, Haluszka, Eugenia, Franchini, Germán, Carreño, Paula, Raboy, Elias, Román, María Dolores, Niclis, Camila, Balangero, Marcos, Altamirano, Natalia, Barbás, María Gabriela, and López, Laura

Abstract

Copyright of Cadernos de Saude Publica is the property of Escola Nacional de Saude Publica Sergio Arouca and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Published
2022
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10. Silent dissemination of HTLV-1 in an endemic area of Argentina. Epidemiological and molecular evidence of intrafamilial transmission

Author

Frutos, María C., primary, Gastaldello, Rene, additional, Balangero, Marcos, additional, Remondegui, Carlos, additional, Blanco, Sebastián, additional, Otsuki, Koko, additional, Paulo Vicente, Ana Carolina, additional, Elías, David, additional, Mangeaud, Arnaldo, additional, Nates, Silvia, additional, and Gallego, Sandra, additional

Published
2017
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13. Retrovirus humanos

Author

Gallego, Sandra Verónica, Gastaldello, René, Balangero, Marcos, Gómez, Lucía, and Castro, Gonzalo Manuel

Subjects
Enfermedades Infecciosas, Retrovirus, HIV, Epidemiología, Virología
Abstract

24 p. La familia Retroviridae incluye una muy amplia variedad de virus capaces de infectar a diferentes especies animales, entre los cuales hay virus que infectan a humanos. La característica principal de los retrovirus desafía al dogma central de la genética molecular, la cual determina que el flujo de la información genética tiene un solo sentido 2032-09-15 Fil: Gallego, Sandra Verónica. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Instituto de Virología "Dr. José María Vanella"; Argentina. Fil: Gastaldello, René. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Instituto de Virología "Dr. José María Vanella"; Argentina. Fil: Balanguero, Marcos. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Instituto de Virología "Dr. José María Vanella"; Argentina. Fil: Gómez, Lucía. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Instituto de Virología "Dr. José María Vanella"; Argentina. Fil: Castro, Gonzalo Manuel. Universidad Nacional de Córdoba. Facultad de Ciencias Médicas. Instituto de Virología "Dr. José María Vanella"; Argentina. Virología

Published
2013

17. HTLV Type 1 Genetic Types among Native Descendants in Argentina

Author

Gastaldello, René, primary, Iñiguez, Alena Mayo, additional, Otsuki, Koko, additional, Lamas, Gabriela, additional, Balangero, Marcos, additional, Barbas, María Gabriela, additional, Mangano, Andrea, additional, Sen, Luisa, additional, Maturano, Eduardo, additional, Remondegui, Carlos, additional, Vicente, Ana Carolina Paulo, additional, and Gallego, Sandra, additional

Published
2008
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18. [A population-based study on seroprevalence and factors associated with SARS-CoV-2 infection in Córdoba, Argentina].

Author

Aballay LR, Coquet JB, Scruzzi GF, Haluszka E, Franchini G, Carreño P, Raboy E, Román MD, Niclis C, Balangero M, Altamirano N, Barbás MG, and López L

Subjects
Adolescent, Antibodies, Viral, Argentina epidemiology, Brazil, Child, Child, Preschool, Cross-Sectional Studies, Humans, Immunoglobulin G, SARS-CoV-2, Seroepidemiologic Studies, COVID-19 epidemiology
Abstract

Seroepidemiological studies help identify the indirect distribution of diseases, detecting serological markers of immunity and demonstrating undiagnosed infections in the general population. The objectives were to estimate the seroprevalence of SARS-CoV-2 antibodies in Córdoba, Argentina, from December 2020 to January 2021 and to identify factors associated with the virus' contagiousness. A population-based cross-sectional observational study was performed in 3,225 individuals over two years of age living in Córdoba city, selected by multiple-stage random sampling proportional to distribution by gender, age group, and socioeconomic status in the city's population. Clinical characteristics, anthropometry, and comorbidities were collected by interview. Qualitative serological testing was performed for detection of SARS-CoV-2 nucleocapsid IgG antibodies (ARCHITECT, Abbott). SARS-CoV-2 seroprevalence was estimated for the total population and by age group, sex, socioeconomic status, and presence of target diseases. Prevalence ratios (PR) were estimated using a log-binomial regression model. SARS-CoV-2 seropositivity was 16.68% (95%CI: 15.41-18.01). Age 2 to 18 years, living in neighborhoods with low socioeconomic status, and obesity increased the odds of seropositivity (PR = 1.50; 95%CI: 1.10-2.04, PR = 1.91; 95%CI: 1.34-2.67 and PR = 1.39; 95%CI: 1.04-1.85). The results indicate that the city of Córdoba displays differential attributes that increase the likelihood of a positive SARS-CoV-2 antibody test. This allows targeting epidemiological surveillance strategies to reduce the spread of the virus.

Published
2022
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19. [Sensitivity of the COBAS AmpliScreen™ HIV-1 test v1.5 for HIV-1 detection].

Author

Gomez LP, Balangero MC, Castro G, Kademian S, Mangeaud A, Barbas MG, Cudolá A, de León JF, Carrizo H, and Gallego SV

Subjects
Argentina epidemiology, Blood Safety, Genotyping Techniques, HIV Infections blood, HIV Infections epidemiology, HIV-1 classification, HIV-1 genetics, Humans, Mass Screening methods, Reagent Kits, Diagnostic, Sensitivity and Specificity, Ultracentrifugation, Viral Load, Colorimetry methods, HIV Infections virology, HIV-1 isolation & purification, RNA, Viral blood, Reverse Transcriptase Polymerase Chain Reaction methods, Viremia virology
Abstract

The introduction of nucleic acid amplification techniques (NAT) in blood banks was intended to reduce the residual risk of transfusion-transmitted infections. Co-circulation of a great diversity of HIV-1 variants in Argentina portrays the need to assess the sensitivity of serological and molecular assays available for their detection. In this study, we evaluated the sensitivity of the COBAS AmpliScreen™ HIV-1 Test, version 1.5 (Roche) for the detection of HIV-1 RNA in plasma samples of infected individuals from Argentina. The results of this study reveal that this technique has high sensitivity for the detection of HIV-1 RNA under assay conditions: using mini-pool testing, pools ≥ 50 RNA copies per ml achieved ≥ 92 % sensitivity, whereas in the standard procedure, samples ≥ 207 RNA copies/ml achieved 100 % sensitivity. Moreover, the COBAS AmpliScreen™ HIV-1 Test, version 1.5 (Roche) is suitable for detecting prevailing HIV-1 variants., (Copyright © 2014 Asociación Argentina de Microbiología. Publicado por Elsevier España. All rights reserved.)

Published
2014
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20. [Serological diagnosis of HTLV-1/2: combination of screening assays to define the serological status in blood donors].

Author

Moreno C, Balangero M, Barbás MG, Cudolá A, and Gallego S

Subjects
Enzyme-Linked Immunosorbent Assay, Humans, Serologic Tests, Antibodies, Viral blood, Blood Donors, Donor Selection methods, HTLV-I Infections blood, HTLV-I Infections diagnosis, HTLV-II Infections blood, HTLV-II Infections diagnosis, Human T-lymphotropic virus 1 immunology, Human T-lymphotropic virus 2 immunology
Abstract

Alternative algorithms were evaluated in order to reduce the number of false reactive results for antibodies against HTLV-1/2. From 20,210 samples tested, 0.37% (74/20,210) was reactive by ELISA Murex. Of these, 23 were confirmed as positive by the indirect immunofluorescence assay whereas 51 were negative, being the positive predictive value (PPV) 31.08%. From a combination of the ELISA Murex assay with the particle agglutination assay (PA) and ELISA MP, the following results were obtained: 26/74 were reactive by ELISA Murex and PA, PPV 88.5% and 32/74 were reactive by ELISA Murex and ELISA MP, PPV 71.8 %. The ROC curve analysis determined that for an RP 4.74, the values for sensitivity, specificity, PPV and NPV by ELISA Murex were 100%, 98.04%, 95.8% and 100%, respectively. We propose that reactive samples by ELISA Murex with an RP d 4.74 should be retested in duplicate by PA, and the resulting concordantly nonreactive samples should be defined as negative for HTLV-1/2.

Published
2013
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