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This study was aimed to determine the phytochemicals and nutritional compositions, antioxidant activity and sensorial properties of Moringa oleifera extracts. The powders prepared from leaves and pods were mixed separately at the ratios of 1:0, 0:1, 0.25:0.75, 0.5:0.5 and 0.75:0.25 and labelled as mixtures A, B, C, D and E, respectively. Mixture A exhibited highest chlorophylls, tannins, phenolics and flavonoids contents (17.8 mg/g, 9.1 mg GAE/g, 91.1 mg GAE/g and 38.1 mg QE/g, respectively). The crude proteins content was higher (18.03%) in mixture A. The fats, fibres and carbohydrates amounts were higher (2.96%, 11.02% and 67.86%, respectively) in mixture B. The highest energy value (335.62 Kcal/100 g) and the highest antioxidant activity (83.2%) were in mixture A. However, most of the sensory attributes were ranked high for mixture D, signifying to use the equal proportion of leaves and pods powder of M. oleifera for the development of food products. [ABSTRACT FROM AUTHOR]

Pectinases are the group of enzymes that catalyze the degradation of pectic substances. It has wide applications in food industries for the production and clarification of wines and juices. The aim of this study was to isolate, screen and characterize pectinase from fungi isolated from various soil samples and evaluate its application in juice clarification. Fungal strains were isolated and screened primarily using 1% citruspectin incorporated potato dextrose agar (PDA) and secondarily using pectinase screening agar medium (PSAM) for pectinolytic organisms. The enzyme was produced by submerged state fermentation and assayed using the dinitro salicylic acid (DNS) method. From 20 different soil samples, 55 fungal isolates were screened primarily and, among them, only 14 isolates were subjected for secondary screening. Out of 14, only four strains showed the highest pectinolytic activity. Among four strains, Aspergillus spp. Gm showed the highest enzyme production at a 48-h incubation period, 1% substrate concentration, and 30°C temperature. The thermal stability assessment resulted that the activity of pectinase enzyme declines by 50% within 10 min of heating at 60°C. The optimum temperature, pH, and substrate concentration for the activity of enzyme was 30°C (75.4 U/mL), 5.8 (72.3 U/mL), and 0.5% (112.0 U/mL), respectively. Furthermore, the yield of the orange juice, the total soluble solid (TSS), and clarity (% transmittance) was increased as the concentration of the pectinase increased, indicating its potential use in juice processing. Overall, the strain Aspergillus spp. Gm was identified as a potent strain for pectinase production in commercial scale. [ABSTRACT FROM AUTHOR]

The government of Nepal has recommended blanket fertilizer application for rice cultivation, which results in lower nutrient use efficiency (NUE) particularly under rainfed conditions. With the aim of finding an appropriate nutrient management practices concerning rice production and profitability, a field experiment was conducted during rainy season of 2017 and 2018 at Kavrepalanchowk and Dang district of Nepal. Altogether, five treatments comprising various nutrient management practices viz. Nutrient Expert Model (NE), use of Leaf Color Chart (LCC), Government Recommended Fertilizer Dose (GON), Farm Yard Manure (FYM), and Farmers' Field Practice (FFP), were laid out in RCBD with four replications in farmers' fields. The analysis of variance showed significant difference between treatments for test weight and grain yield in Kavrepalanchowk whereas all traits except number of effective tillers were significant in Dang. The significantly higher grain yield and harvest index were obtained in NE, followed by LCC; and the overall straw yield was highest in LCC, followed by NE in both the locations. Also, yield gap analysis suggested the NE had 44.44% and 23.97% increase in yield as compared to FPP in Kavrepalanchowk and Dang, respectively. The combined analysis with Best Linear Unbiased Estimator revealed the interaction of nutrient management and location significantly effects the straw yield and harvest index across both the locations. The estimated mean straw yield and harvest index were 10.93 t/ha and 34.98%, respectively. Both correlation study and biplot of principal component analysis signaled grain yield had positive correlation with all other traits. Furthermore, the net revenue was maximum for NE, followed by LCC in both the locations. The benefit: cost ratio was highest for NE which was 1.55 in Kavrepalanchowk and 2.61 in Dang. On the basis of these findings, NE and LCC can be effectively used as nutrient management practice by the farmers to obtain maximum production and profitability in Rice. [ABSTRACT FROM AUTHOR]