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High cost platinum (Pt) catalysts limit the application of microbial electrolysis cells (MECs) for hydrogen (H

Plastics waste and microplastics including polyethylene (PE) and polystyrene (PS) have been an environmental concern for years. Recent research has revealed that larvae of

Plastic biodegradation by mealworm is regarded as an emerging strategy for plastic disposal. In this study, the polystyrene (PS) and low density polyethylene (LDPE) degradation efficiency by yellow mealworms (Tenebrio molitor larvae) supplemented with bran and the effects of plastics on the gut core microbiome were explored to construct a circular and continuous reactor for plastic biodegradation in the future. The gut microbiome was also investigated with dietary shift to explore the relationship between specific diets and gut microbes. The bran plus plastic (7:1 ratio, w/w) co-diet contributed to the mealworm survival and growth. The formation of −C˭O−/−C−O− groups in the plastic-fed mealworms frass represented the oxidation process of plastic biodegradation in the mealworm gut. The changes in molecular weights (Mw, Mn and Mz) of residual PS and LDPE in mealworms frass compared with that of PS and PE feedstock confirmed the plastic depolymerization and biodegradation. Lactobacillus and Mucispirillum were significantly associated with PE + bran diet compared to bran diet and PE diet, representing the response of mealworm gut microbiome to the bran and plastic mixture was distinguished from either bran or plastics alone. The gut microbiome changed substantially with the diet shift, indicating that microbial community assembly was a stochastic process and diverse plastic-degrading bacteria might occur in the mealworm gut.

Anaerobic co-digestion of a cow manure-cotton straw mixture (CCM) has been shown to promote methanogenesis, but the recalcitrant crystal structure of organic polymers in CCM hinders its hydrolysis during anaerobic digestion (AD). Here, the efficacy of different pretreatment methods based on potassium ferrate (PF) and peroxymonosulfate (PMS) was evaluated to facilitate CCM decomposition and methanogenesis during AD. The maximum lignocellulosic removal rate (62.5%), the highest volatile fatty acids (VFAs) (7769.6 mg/L), and cumulative methane yield (109.4 mL CH4/g VS) were both achieved in PF-pretreated samples after the digestion process. The dominant bacterial populations in PF-pretreated CCM were affiliated with Sideroxydans, Herbinix, Clostridium, and Smithella, which played an important role in the hydrolysis and acidification of CCM. The enrichment of Methanosarcina and Methanobacterium and highly-effective acidogenesis might account for the highest methane yield in the PF-pretreated group.

Complete-nitrifying bacteria (comammox) play important roles in nitrogen-overloading aquatic systems. However, the understanding of the environmental relevance is still limited. Here, we studied the responses of comammox bacteria (Nitrospira inopinata) in a tributary of the Yellow River, with the water and sediment, microbial, seasonal, and chemical variations considered. Illumina sequencing indicated that the predominant phyla in the river sediment were Proterobacteria, Bacteroidetes, Actinobacteria, and Chloroflex. Quantitative PCR revealed that N. inopinata-like comammox were approximately twice as abundant in the water during the wet season and in the sediment during the dry season than that of other conditions. Significant correlations were found between the abundance of N. inopinata-like comammox and pH (r = 0.58), temperature (r = 0.63), and dissolved oxygen (r = - 0.77). The abundance of N. inopinata-like comammox was higher than that of ammonia oxidizing archaea (AOA), and lower than that of ammonia oxidizing bacteria (AOB) and nitrite oxidizing bacteria (NOB). Furthermore, a significant correlation was discovered between N. inopinata-like comammox and NOB (r = 0.60), and so was anammox bacteria (r = 0.358). Interestingly, N. inopinata-like comammox also showed positive relationships with denitrifying microbes (r = 0.559).