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Introduction The Psychotropic Drug Safety Initiative (PDSI) is a national Veterans Affairs program that recommends obtaining cardiovascular vital signs semiannually and urine toxicology screening annually for veterans prescribed stimulants. The PDSI also recommends a risk review of concurrent central nervous system (CNS) depressants to ensure the benefits of coadministration with stimulants outweigh the risks. This project’s purpose was to evaluate the occurrence of coprescriptions for CNS depressants and stimulants and encourage compliance with the PDSI recommendations to increase safe and appropriate management of veterans prescribed the combination. This study aimed to evaluate the occurrence of coprescriptions for CNS depressants and stimulants, evaluate compliance with stimulant monitoring recommendations, and measure the proportion of pharmacist recommendations implemented by the prescriber. Methods This quality improvement project identified veterans with an outpatient prescription for a stimulant and any coprescription(s) for benzodiazepines, sedative-hypnotics, and/or opioids. A pharmacy intervention note was generated to request a risk review, provide recommendations for de-escalation, and notify the stimulant prescriber of overdue monitoring parameters. Impact was measured 60 days after intervention. Descriptive statistics and a McNemar test were used to compare preintervention and postintervention data. Results From the 61 patients included, there were 67 unique prescriptions for benzodiazepines (49.3%), sedative-hypnotics (34.3%), and opioids (16.4%) in combination with a stimulant. Pharmacist intervention resulted in de-escalation of coprescribing for 9 patients (16.1%) and was associated with statistically significant improvement in compliance to stimulant monitoring recommendations. Discussion Clinical pharmacists can assist in ensuring safe and appropriate monitoring and management of veterans prescribed stimulants.

Oligodendrocyte-lineage cells are central nervous system (CNS) glia that perform multiple functions including the selective myelination of some but not all axons. During myelination, synaptic vesicle release from axons promotes sheath stabilization and growth on a subset of neuron subtypes. In comparison, it is unknown if pre-myelinating oligodendrocyte process extensions selectively interact with specific neural circuits or axon subtypes, and whether the formation and stabilization of these neuron–glia interactions involves synaptic vesicle release. In this study, we used fluorescent reporters in the larval zebrafish model to track pre-myelinating oligodendrocyte process extensions interacting with spinal axons utilizing in vivo imaging. Monitoring motile oligodendrocyte processes and their interactions with individually labeled axons revealed that synaptic vesicle release regulates the behavior of subsets of process extensions. Specifically, blocking synaptic vesicle release decreased the longevity of oligodendrocyte process extensions interacting with reticulospinal axons. Furthermore, blocking synaptic vesicle release increased the frequency that new interactions formed and retracted. In contrast, tracking the movements of all process extensions of singly-labeled oligodendrocytes revealed that synaptic vesicle release does not regulate overall process motility or exploratory behavior. Blocking synaptic vesicle release influenced the density of oligodendrocyte process extensions interacting with reticulospinal and serotonergic axons, but not commissural interneuron or dopaminergic axons. Taken together, these data indicate that alterations to synaptic vesicle release cause changes to oligodendrocyte-axon interactions that are neuron subtype specific.

Abstract The HIV-1 fusion peptide (FP) represents a promising vaccine target, but global FP sequence diversity among circulating strains has limited anti-FP antibodies to ~60% neutralization breadth. Here we evolve the FP-targeting antibody VRC34.01 in vitro to enhance FP-neutralization using site saturation mutagenesis and yeast display. Successive rounds of directed evolution by iterative selection of antibodies for binding to resistant HIV-1 strains establish a variant, VRC34.01_mm28, as a best-in-class antibody with 10-fold enhanced potency compared to the template antibody and ~80% breadth on a cross-clade 208-strain neutralization panel. Structural analyses demonstrate that the improved paratope expands the FP binding groove to accommodate diverse FP sequences of different lengths while also recognizing the HIV-1 Env backbone. These data reveal critical antibody features for enhanced neutralization breadth and potency against the FP site of vulnerability and accelerate clinical development of broad HIV-1 FP-targeting vaccines and therapeutics.

(1) Background: Endometriosis is characterized by the presence of endometrial glands and stroma outside of the uterus and is often associated with severe pelvic pain and infertility. Our study explored the utilization of B-Cell Lymphoma 6 (BCL6) and Sirtuin 1 (SIRT1) as potential biomarkers in serum, plasma, urine, and cervical mucus for a non-invasive diagnostic test for endometriosis. BCL6 was chosen based on its previously reported elevated expression in endometrial biopsies, and SIRT1 is co-expressed and upregulated in the endometrium of women with endometriosis. (2) Methods: BCL6 and SIRT1 levels were measured using enzyme-linked immunoassay (ELISA) in samples from 20 women with endometriosis (ten with stages I/II and ten with stages III/IV) and ten women without endometriosis. (3) Results: Levels of SIRT1 in sera showed a statistically significant elevation in advanced stages III/IV compared to controls and stages I/II. No significant differences were found in other bodily fluids for SIRT1 or any bodily fluids tested for BCL6. (4) Conclusions: These results suggest some potential of SIRT1 expression within serum as a predictor of advanced asymptomatic stages of endometriosis. Using immunohistochemistry (IHC) staining and H-SCORE values for the elevated BCL6 (and potentially SIRT1) levels in endometrial biopsy samples seems to have higher diagnostic potential based on the previously published studies.

Summary: Understanding mechanisms of protective antibody recognition can inform vaccine and therapeutic strategies against SARS-CoV-2. We report a monoclonal antibody, 910-30, targeting the SARS-CoV-2 receptor-binding site for ACE2 as a member of a public antibody response encoded by IGHV3-53/IGHV3-66 genes. Sequence and structural analyses of 910-30 and related antibodies explore how class recognition features correlate with SARS-CoV-2 neutralization. Cryo-EM structures of 910-30 bound to the SARS-CoV-2 spike trimer reveal binding interactions and its ability to disassemble spike. Despite heavy-chain sequence similarity, biophysical analyses of IGHV3-53/3-66-encoded antibodies highlight the importance of native heavy:light pairings for ACE2-binding competition and SARS-CoV-2 neutralization. We develop paired heavy:light class sequence signatures and determine antibody precursor prevalence to be ∼1 in 44,000 human B cells, consistent with public antibody identification in several convalescent COVID-19 patients. These class signatures reveal genetic, structural, and functional immune features that are helpful in accelerating antibody-based medical interventions for SARS-CoV-2.

This article presents the institutional and historical context, methods, findings, and action-consequences of 'Our HMoob American College Paj Ntaub,' a qualitative, student-led Participatory Action Research (PAR) project documenting the sociocultural and institutional factors that influence HMoob American college students' experiences at the University of Wisconsin-Madison. Drawing on concepts from Asian Critical Race Theory (AsianCrit), we identify sociocultural and institutional processes that misrepresent and erase HMoob American experiences, producing a profound and troubling experience of institutional invisibility which has serious consequences for students' wellbeing and educational attainment. However, we also document that student-led PAR research can be an effective means of enacting what we term 'counter-invisibility work,' by producing compelling counter-narratives that expand social networks for activism, outreach, and policy enactment.

In the field of network administration and programming, mastery of technical skills as well as non-technical or soft skills, such as teamwork, problem-solving, self-regulated learning, and communication, are increasingly emphasized both in practice and research. While little research exists concerning the instructional practices of such skills within community colleges, even less is known about community college instructors' conceptions of these crucial 21st century skills. This paper reports a qualitative, exploratory study examining how community college instructors defined and taught communication and teamwork competencies in their computer technology classrooms. The data was collected from multiple data sources -- instructor interviews, observations of classroom teaching, and student focus group discussions about their learning. Main findings include the influence of instructor backgrounds on their teaching practices, the situational nature of how instructors defined and conceptualized communication and teamwork skills, and the prominent use of teacher-centered practices in the teaching of communication and teamwork skills. We conclude that while faculty participants in this study considered teamwork and communication skills to be important, their classroom teaching was insufficiently student-centered to truly cultivate these skills. Thus, we argue that more training, resources, and support structures are needed for community college instructors in order to better cultivate the communication and teamwork skills of their students. This paper adds empirical evidence to the national conversations on the critical issues of skills, student employment, and pedagogy, and also utilizes a triangulating approach to better document faculty teaching practices.

What is it about afterschool that gives it so much potential to powerfully influence educational best practices around the world? This paper will explore what truly defines "afterschool" beyond the time- and location-based pedagogy of the term and will make the case for the use of "expanded learning" or "expanded opportunities" as the terms that most clearly describe this critical time of youth learning and development. This paper will explore the cultural and bureaucratic differences countries have that influence how young people spend their time and what those differences say about a country's views on child development and its aspirations for its youth. Beyond an exploration of cultural differences, this paper will also discuss the trends that are influencing our international efforts to shift the view of expanded learning programs beyond a safe place to keep children occupied while their parents work, toward a fundamental space for child development and educational innovation.

Over the past 35 years, a range of 'new' policy actors and networks has emerged from the private sector under the rationale of efficient, free-market alternatives to traditional public policymakers. Under a critical policy studies framework, this paper uses discourse analysis and interviews with key stakeholders to examine how one regional workforce development initiative -- the UpSkill Houston initiative -- promotes a business-driven vision of education reform. We find that their programmatic narratives promote this vision through a focus on closing the 'skills gap' and tailoring education to best meet the labor needs of regional industries. We further note that UpSkill Houston espouses a skills-based vision of a worker pipeline directly from the education sector into high-growth regional industries. We situate UpSkill Houston's programmatic narrative within an expanding number of private regional workforce development initiatives that seek to leverage postsecondary public education resources to support regional business interests. We argue that initiatives such as UpSkill Houston are important to investigate, as they are among an increasing trend of new education policy actors and networks that exert control over education policy.

Emotionally salient experiences are encoded and remembered more strongly, an effect that can be amplified by hormones like cortisol. Such memories can in turn profoundly influence later behavior. However, little is known about the link between amplified salience encoding and subsequent behavior. This pathway may be particularly important for risky alcohol drinking, which has been linked to sensitized salience responses, memory, and cortisol. To test this possibility, we integrated pharmacology using a double-blind cross-over design with fMRI, cognitive, and motivation assays across a range of healthy male and female social drinkers. As anticipated, cortisol enhanced memory for salient alcohol-related events; critically, this bias was in turn associated with later alcohol motivation. Increased alcohol motivation was particularly pronounced in more susceptible risky drinkers, for whom cortisol enhanced brain salience responses to alcohol. These sensitized salience responses predicted both memory biases and alcohol motivation. Together, these findings reveal maladaptive consequences of enhanced salience encoding [ABSTRACT FROM AUTHOR]

This article uses ethnographic data to examine the schooling experiences of Latinx emergent bilinguals and the educators who worked with them at a mid-size high school in a small Wisconsin city. Using Critical Race Theory, I argue that as tensions have increased in recent years from school funding cuts, deindustrialization, and demographic shifts, the community's white residents have repeated long-standing patterns of white supremacy to assert economic and cultural dominance over Communities of Color. I examine the manifestation of these dynamics in the schooling experiences of my participants through a racially hostile schooling environment and a general lack of investment in their education. This work offers important insights on the sociohistorical intersections of race and class within predominantly white, rural and suburban 'new destination' communities and problematizes the very notion of 'new' in such locals by questioning assumptions about who is 'native' and who is 'new' to a particular community

One of the problematic features of the "skills discourse" is the view that skills are decontextualized bits of knowledge and disposition. Instead, how skills such as communication are defined and used are shaped by cultural, political, and situational factors. In this article, we integrate theory from communication studies, critical discourse analysis, and cognitive anthropology to examine how 96 students, educators, and employers in nursing and engineering define and describe communication skills. Thematic and social network analyses revealed multiple "genres" of communication, their association with specific situations, variation in the structure of situated notions of communication by role group, and cultural models underlying their use. Results suggest new approaches for research and practice regarding teaching and cultural diversity in higher education.

In the Southern California Bight, the common dolphin Delphinus delphis is the most abundant dolphin species and preys upon small pelagic fish, mesopelagic fish, and cephalopods. Mesopelagic fish and many cephalopods are available throughout the year, and they form deep scattering layers, some of which characteristically undergo strong diel vertical migrations. The extent of vertical migration depends on the degree of sea surface solar and lunar illumination. At their daytime depth, mesopelagic prey are beyond the range of shallow-diving dolphins. Autonomous acoustic recorders were used tomonitor dolphin echolocation at 2 offshore recording locations from 2009 to 2014. Manual and automated classification techniques were used to identify periods of high echolocation activity, indicative of common dolphin foraging. Clear lunar patterns existed in cool months, when echolocation activity was highest during the darkest periods of the night and lunar month, indicating times when dolphins were foraging, possibly on mesopelagic prey. Echolocation was more abundant during warmmonths, but diel and lunar patterns in echolocation were weaker. Generalized additive mixed models show that the observed patterns in echolocation activity are correlated with lunar day and position of the moon in the night sky. Seasonal patterns may represent geographic shifts in common dolphin populations, shoaling scattering layers, or prey switching behavior during the warmmonths, whereby dolphins target small pelagic fish not associated with the deep scattering layers. Overall, dolphin foraging activity declined from 2009 to 2014 during warm months, which may be related to a declining abundance of small pelagic fish.

Graduate employability has increasingly become the key measure of a college and university's value. The focus on the economic returns to education is much more than an American trend, as postsecondary leaders all over the world are increasingly preoccupied with proving to skeptical legislators that their students are getting jobs after they graduate. One particular set of skills are at the very heart of debates about employability: the so-called "soft skills." These are the social, attitudinal, and self-regulatory competencies or traits that allow effective communication, working well with others, and persistence in the face of adversity. Based on the notion that the purpose of a college education is primarily (if not solely) to instill in students workplace-ready abilities and that competencies such as teamwork, critical thinking, and communication are especially desired by employers, the "Soft Skills Paradigm," is shaping public perception and education policy around the world. No longer a point of much contention; it has become common sense. The authors however believe that the influential logic of the "Soft Skills Paradigm," is detrimental to the long-term interests of the students who trust colleges and universities with their futures. They posit that the "Soft Skills Paradigm" is also damaging to those in the professions who seek skilled young people proficient in their disciplinary cultures and practices. In this era of growing racial and cultural intolerance, the authors contend that the "Soft Skills Paradigm" also reinforces the deficit model of achievement in higher education, effectively blaming students for their lack of employability and soft skills while ignoring the structural, cultural, and socio-economic forces that thwart mobility and access. The authors' critique in this article focuses on how the paradigm reflects a decontextualized and inaccurate vision of human competence as well as the role of learned skill in society and the workplace. By comparison, the "Soft Skills Paradigm" views skills as generic, context-independent, and simple to teach and learn. It overlooks how normative views of skills are constructed by those in positions of power, often marginalizing notions of skill from minoritized groups. In contrast, the authors turned to another way to consider the issues implicated in the debates about skills, college, and jobs. The "Cultural Capital Paradigm," views human competency as complex, grounded in professional norms, difficult to teach and learn, and differentially valued depending on the profession and situation. As proponents of the Cultural Capital Paradigm, the authors stress the notion that human competencies are: (1) varied, complex and interconnected; (2) assigned value in specific social, professional, political, and cultural contexts, and (3) are often used as gatekeeping mechanisms to include or exclude certain individuals from obtaining prestige and position. The article calls for a new approach to thinking about college students' skills and employability, and a paradigm shift from the "Soft Skills Paradigm," to the more nuanced realistic and humanistitic perspective -- the "Cultural Capital Paradigm." The article outlines the authors' perspective in detail, and argues for a change in current conversations about students' employability and skills. A table provides a side by side comparison of the differences between the two paradigms.

This interview-based study explores the nature of reflective practice among postsecondary faculty by examining the types of teaching-related data faculty use during their reflection, their reflective practice process, and the contextual factors that influence that process. Our findings indicate faculty drew on both numeric and non-numeric data forms to engage in reflective practice which complicates the current imagination of "data" within the Data-Driven Decision Making (DDDM) movement. Our findings also showed three distinct types of faculty reflection - instrumental, structural-critical, and social-critical - which demonstrate the varied functions and forms reflection can take. Finally, we demonstrate that although faculty consistently engaged in reflective practice, the outcomes of this reflection were severely limited by both individual bias and institutional constraints. Thus, while we recognize the current budgetary struggles many universities are facing, we argue that in order to better serve postsecondary students, particularly those from historically underrepresented groups, more institutional support is needed. Specifically, we argue postsecondary institutions play a significant role in facilitating critical examination by providing faculty the necessary space, time, and guidance to engage in critical reflection as well as the appropriate institutional mechanisms to voice concerns and enact change.

The Advanced Light Source (ALS) at Berkeley Lab remains one of the brightest sources for soft x-rays worldwide. A multiyear upgrade of the ALS is underway, which includes new and replacement x-ray beamlines, a replacement of many of the original insertion devices and many upgrades to the accelerator. The accelerator upgrade that affects the ALS performance most directly is the ALS brightness upgrade [1], which reduces the horizontal emittance from 6.3 to 2.0 nm (2.5 nm effective). Magnets for this upgrade were installed in late 2012 and early 2013 followed by user operation with the reduced emittance.

The Advanced Light Source (ALS) is a thirdgeneration light source in operation since 1993. This light source is providing state-of-the-art performance to more than 40 beamlines and their users, due to the upgrades that have been completed over the last few years. The storage ring upgrade project that is developed here will allow the ALS to provide the 40 beamline users with higher photon beam brightness (factor of 2 or 3) by having its storage ring lattice modified. Forty-eight harmonic sextupole magnets with integrated dipole correctors and skew quadrupole coils will be introduced, which will require a level of installation activity not seen at the ALS since its original construction in 1991. Introducing new combined-function magnets in an existing storage ring is a challenge due to the limited space available and a balance had to be found between magnet performance and spatial constraints. After an introduction reviewing the characteristics of the three design families of the 48 combined-function magnets, the magnet fabrication and installation are developed along with analyses based on the magnetic measurements and the ALS storage ring commissioning results.

The Advanced Light Source (ALS) at Berkeley Lab remains one of the brightest sources for soft x-rays worldwide. A multiyear upgrade of the ALS is underway, which includes new and replacement x-ray beamlines, a replacement of many of the original insertion devices and many upgrades to the accelerator. The accelerator upgrade that affects the ALS performance most directly is the ALS brightness upgrade, which reduced the horizontal emittance from 6.3 to 2.0 nm (2.5 nm effective). Magnets for this upgrade were installed starting in 2012 followed by a transition to user operations with 2.0 nm emittance in spring 2013. Copyright © 2013 by JACoW.

The Advanced Light Source (ALS) at Berkeley Lab while one of the earliest 3rdgeneration light sources remains one of the brightest sources for soft x-rays worldwide. Amultiyear upgrade of the ALS is currently under way, which includes new and replacementx-ray beamlines, a replacement of many of the original insertion devices and many upgradesto the accelerator. The accelerator upgrade that affects the ALS performance most directlyis the ALS brightness upgrade, which will reduce the horizontal emittance from 6.3 to 2.2nm (2.6 nm effective). This will result in a brightness increase by a factor of three forbendmagnet beamlines and at least a factor of two for insertion device beamlines. Magnetsfor this upgrade are currently in production and will be installed starting later thisyear. Copyright © 2012 by IEEE.

The Advanced Light Source is one of the earliest 3rd generation light sources. With an active upgrade program it has remained competitive over the years. The latest in a series of upgrades is a lattice upgrade project that was started in 2009. When it will be completed, the ALS will operate with a horizontal emittance of 2.2 nm and an effective emittance of 2.6 nm. Combined with the high current of 500 mA and the small vertical emittance the ALS already operates at, this upgrade will keep it competitive for years to come. This paper summarizes the status of the upgrade, including beam dynamics studies and lattice optimizations as well as the magnet design.

The fabrication and installation at the Lawrence Berkeley National Laboratory of a high-brightness high-repetition rate photo-gun, based on a normal conducting 187 MHz (VHF) RF cavity operating in CW mode, is in an advanced phase. The cavity will generate an electric field at the cathode plane of ∼ 20 MV/m to accelerate the electron bunches up to ∼ 750 keV, with peak current, energy spread and transverse emittance suitable for FEL and ERL applications. The gun vacuum system has been designed for achieving pressures compatible with the use of "delicate" high quantum efficiency semiconductor cathodes to generate up to a nC bunches at MHz repetition rate with present laser technology. Several photo-cathode/laser systems are under consideration, and in particular photo-cathodes based on K2CsSb are being developed for the gun and have already achieved a QE of 8% at 532 nm wavelength, or close to 20% including the Schottky barrier lowering. The cathode will be operated by a μJ fiber laser in conjunction with refractive transverse beam shaping to create a flat top transverse profile, as well as a birefringent pulse stacker to create a flat top temporal profile. The present status and the plan for future activities are presented.

The Advanced Light Source is currently being upgraded for top-off operation. This major facility upgrade will provide an improvement in brightness from soft x-ray undulators of about one order of magnitude and keep the ALS competitive with the newest intermediate energy light sources. Major components of the upgrade include making the booster synchrotron capable of full energy operation, radiation safety studies, improvements to interlocks and collimation systems, diagnostics upgrades as well as emittance improvements in the main storage ring. Most hardware necessary as part of the upgrade has been installed and commissioned. The radiation safety studies are making good progress and have passed a first outside peer review succesfully. ©2007 IEEE.

The Advanced Light Source is currently being upgraded for top-off operation. This major facility upgrade will provide an improvement in brightness from soft x-ray undulators of about one order of magnitude and keep the ALS competitive with the newest intermediate energy light sources. Major components of the upgrade include making the booster synchrotron capable of full energy operation, radiation safety studies, improvements to interlocks and collimation systems, diagnostics upgrades as well as emittance improvements in the main storage ring. Most hardware necessary as part of the upgrade has been installed and commissioned. The radiation safety studies are making good progress and have passed a first outside peer review succesfully. ©2007 IEEE.