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The urban watershed of Guwahati situated on the bank of the Brahmaputra River is one of the fastest growing cities of India. During the last two decades, water security concerns due to climatic variabilities have become a pronounced issue in the urban watershed of Guwahati. Thus, the study aims to calculate the long-term temporal trends of temperature, precipitation, extreme climate indices, and river discharge to assess the variations and patterns of hydro-climatic variations in the urban watershed of Guwahati from 1991 to 2019. Furthermore, the current study also tries to correlate these extreme climatic indices to river discharge to determine the degree of hydro-climatic variations. The Mann-Kendall statistical techniques and Sen's estimator were used to calculate the statistical significance, stability, and averaged magnitude of trends in the hydro-meteorological data. The result shows that the wetness indices, R20 and RX5Day, reported a decline in Guwahati's urban watershed from 1991 to 2019, resulting in a reduction in intensity and duration of heavy rainfalls while the dry spell (CDD) has been more distinct in the study area with a rise in the average temperature by 0.023 °C/year. Similarly, the most significant statistical trend was found in the monsoonal discharge of the Brahmaputra with a negative trend of - 204.16 m 3 /s/year. The results also show that fluctuations in rainfall patterns have a direct impact on the discharge of the Brahmaputra. These phenomena can affect the quantity of river water resulting in a severe impact on water security in the study area., (© 2021. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)