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354 results on '"Baidoo, Edward E. K."'

1. Neurospora intermedia from a traditional fermented food enables waste-to-food conversion

Author

Maini Rekdal, Vayu, Villalobos-Escobedo, José Manuel, Rodriguez-Valeron, Nabila, Olaizola Garcia, Mikel, Prado Vásquez, Diego, Rosales, Alexander, Sörensen, Pia M., Baidoo, Edward E. K., Calheiros de Carvalho, Ana, Riley, Robert, Lipzen, Anna, He, Guifen, Yan, Mi, Haridas, Sajeet, Daum, Christopher, Yoshinaga, Yuko, Ng, Vivian, Grigoriev, Igor V., Munk, Rasmus, Wijaya, Christofora Hanny, Nuraida, Lilis, Damayanti, Isty, Cruz-Morales, Pablo, and Keasling, Jay. D.

Published
2024
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2. Engineered reduction of S-adenosylmethionine alters lignin in sorghum

Author

Tian, Yang, Gao, Yu, Turumtay, Halbay, Turumtay, Emine Akyuz, Chai, Yen Ning, Choudhary, Hemant, Park, Joon-Hyun, Wu, Chuan-Yin, De Ben, Christopher M., Dalton, Jutta, Louie, Katherine B., Harwood, Thomas, Chin, Dylan, Vuu, Khanh M., Bowen, Benjamin P., Shih, Patrick M., Baidoo, Edward E. K., Northen, Trent R., Simmons, Blake A., Hutmacher, Robert, Atim, Jackie, Putnam, Daniel H., Scown, Corinne D., Mortimer, Jenny C., Scheller, Henrik V., and Eudes, Aymerick

Published
2024
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4. Complete biosynthesis of QS-21 in engineered yeast

Author

Liu, Yuzhong, Zhao, Xixi, Gan, Fei, Chen, Xiaoyue, Deng, Kai, Crowe, Samantha A., Hudson, Graham A., Belcher, Michael S., Schmidt, Matthias, Astolfi, Maria C. T., Kosina, Suzanne M., Pang, Bo, Shao, Minglong, Yin, Jing, Sirirungruang, Sasilada, Iavarone, Anthony T., Reed, James, Martin, Laetitia B. B., El-Demerdash, Amr, Kikuchi, Shingo, Misra, Rajesh Chandra, Liang, Xiaomeng, Cronce, Michael J., Chen, Xiulai, Zhan, Chunjun, Kakumanu, Ramu, Baidoo, Edward E. K., Chen, Yan, Petzold, Christopher J., Northen, Trent R., Osbourn, Anne, Scheller, Henrik, and Keasling, Jay D.

Published
2024
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6. Complete integration of carbene-transfer chemistry into biosynthesis

Author

Huang, Jing, Quest, Andrew, Cruz-Morales, Pablo, Deng, Kai, Pereira, Jose Henrique, Van Cura, Devon, Kakumanu, Ramu, Baidoo, Edward E. K., Dan, Qingyun, Chen, Yan, Petzold, Christopher J., Northen, Trent R., Adams, Paul D., Clark, Douglas S., Balskus, Emily P., Hartwig, John F., Mukhopadhyay, Aindrila, and Keasling, Jay D.

Published
2023
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8. Lignin deconstruction by anaerobic fungi

Author

Lankiewicz, Thomas S., Choudhary, Hemant, Gao, Yu, Amer, Bashar, Lillington, Stephen P., Leggieri, Patrick A., Brown, Jennifer L., Swift, Candice L., Lipzen, Anna, Na, Hyunsoo, Amirebrahimi, Mojgan, Theodorou, Michael K., Baidoo, Edward E. K., Barry, Kerrie, Grigoriev, Igor V., Timokhin, Vitaliy I., Gladden, John, Singh, Seema, Mortimer, Jenny C., Ralph, John, Simmons, Blake A., Singer, Steven W., and O’Malley, Michelle A.

Published
2023
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15. Further engineering of R. toruloides for the production of terpenes from lignocellulosic biomass

Author

Kirby, James, Geiselman, Gina M., Yaegashi, Junko, Kim, Joonhoon, Zhuang, Xun, Tran-Gyamfi, Mary Bao, Prahl, Jan-Philip, Sundstrom, Eric R., Gao, Yuqian, Munoz, Nathalie, Burnum-Johnson, Kristin E., Benites, Veronica T., Baidoo, Edward E. K., Fuhrmann, Anna, Seibel, Katharina, Webb-Robertson, Bobbie-Jo M., Zucker, Jeremy, Nicora, Carrie D., Tanjore, Deepti, Magnuson, Jon K., Skerker, Jeffrey M., and Gladden, John M.

Published
2021
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17. Production of hydroxycinnamoyl anthranilates from glucose in Escherichia coli

Author

Eudes, Aymerick, Juminaga, Darmawi, Baidoo, Edward E K, Collins, F, Keasling, Jay D, and Loqué, Dominique

Abstract

Abstract Background Oats contain hydroxycinnamoyl anthranilates, also named avenanthramides (Avn), which have beneficial health properties because of their antioxidant, anti-inflammatory, and antiproliferative effects. The microbial production of hydroxycinnamoyl anthranilates is an eco-friendly alternative to chemical synthesis or purification from plant sources. We recently demonstrated in yeast (Saccharomyces cerevisiae) that coexpression of 4-coumarate: CoA ligase (4CL) from Arabidopsis thaliana and hydroxycinnamoyl/benzoyl-CoA/anthranilate N-hydroxycinnamoyl/benzoyltransferase (HCBT) from Dianthus caryophyllusenabled the biological production of several cinnamoyl anthranilates upon feeding with anthranilate and various cinnamates. Using engineering strategies to overproduce anthranilate and hydroxycinnamates, we describe here an entire pathway for the microbial synthesis of two Avns from glucose in Escherichia coli. Results We first showed that coexpression of HCBT and Nt4CL1 from tobacco in the E. coli anthranilate-accumulating strain W3110 trpD9923 allowed the production of Avn D [N-(4′-hydroxycinnamoyl)-anthranilic acid] and Avn F [N-(3′,4′-dihydroxycinnamoyl)-anthranilic acid] upon feeding with p-coumarate and caffeate, respectively. Moreover, additional expression in this strain of a tyrosine ammonia-lyase from Rhodotorula glutinis (RgTAL) led to the conversion of endogenous tyrosine into p-coumarate and resulted in the production of Avn D from glucose. Second, a 135-fold improvement in Avn D titer was achieved by boosting tyrosine production using two plasmids that express the eleven genes necessary for tyrosine synthesis from erythrose 4-phosphate and phosphoenolpyruvate. Finally, expression of either the p-coumarate 3-hydroxylase Sam5 from Saccharothrix espanensis or the hydroxylase complex HpaBC from E. coli resulted in the endogenous production of caffeate and biosynthesis of Avn F. Conclusion We established a biosynthetic pathway for the microbial production of valuable hydroxycinnamoyl anthranilates from an inexpensive carbon source. The proposed pathway will serve as a platform for further engineering toward economical and sustainable bioproduction of these pharmaceuticals and other related aromatic compounds.

Published
2013

19. Complete biosynthesis of cannabinoids and their unnatural analogues in yeast

Author

Luo, Xiaozhou, Reiter, Michael A., d’Espaux, Leo, Wong, Jeff, Denby, Charles M., Lechner, Anna, Zhang, Yunfeng, Grzybowski, Adrian T., Harth, Simon, Lin, Weiyin, Lee, Hyunsu, Yu, Changhua, Shin, John, Deng, Kai, Benites, Veronica T., Wang, George, Baidoo, Edward E. K., Chen, Yan, Dev, Ishaan, Petzold, Christopher J., and Keasling, Jay D.

Published
2019
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20. An iron (II) dependent oxygenase performs the last missing step of plant lysine catabolism

Author

Thompson, Mitchell G., Blake-Hedges, Jacquelyn M., Pereira, Jose Henrique, Hangasky, John A., Belcher, Michael S., Moore, William M., Barajas, Jesus F., Cruz-Morales, Pablo, Washington, Lorenzo J., Haushalter, Robert W., Eiben, Christopher B., Liu, Yuzhong, Skyrud, Will, Benites, Veronica T., Barnum, Tyler P., Baidoo, Edward E. K., Scheller, Henrik V., Marletta, Michael A., Shih, Patrick M., Adams, Paul D., and Keasling, Jay D.

Published
2020
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21. Expanding Extender Substrate Selection for Unnatural Polyketide Biosynthesis by Acyltransferase Domain Exchange within a Modular Polyketide Synthase

Author

Englund, Elias, primary, Schmidt, Matthias, additional, Nava, Alberto A., additional, Lechner, Anna, additional, Deng, Kai, additional, Jocic, Renee, additional, Lin, Yingxin, additional, Roberts, Jacob, additional, Benites, Veronica T., additional, Kakumanu, Ramu, additional, Gin, Jennifer W., additional, Chen, Yan, additional, Liu, Yuzhong, additional, Petzold, Christopher J., additional, Baidoo, Edward E. K., additional, Northen, Trent R., additional, Adams, Paul D., additional, Katz, Leonard, additional, Yuzawa, Satoshi, additional, and Keasling, Jay D., additional

Published
2023
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22. Expanding Extender Substrate Selection for Unnatural Polyketide Biosynthesis by Acyltransferase Domain Exchange within a Modular Polyketide Synthase

Author

Englund, Elias, Schmidt, Matthias, Nava, Alberto A., Lechner, Anna, Deng, Kai, Jocic, Renee, Lin, Yingxin, Roberts, Jacob, Benites, Veronica T., Kakumanu, Ramu, Gin, Jennifer W., Chen, Yan, Liu, Yuzhong, Petzold, Christopher J., Baidoo, Edward E. K., Northen, Trent R., Adams, Paul D., Katz, Leonard, Yuzawa, Satoshi, Keasling, Jay D., Englund, Elias, Schmidt, Matthias, Nava, Alberto A., Lechner, Anna, Deng, Kai, Jocic, Renee, Lin, Yingxin, Roberts, Jacob, Benites, Veronica T., Kakumanu, Ramu, Gin, Jennifer W., Chen, Yan, Liu, Yuzhong, Petzold, Christopher J., Baidoo, Edward E. K., Northen, Trent R., Adams, Paul D., Katz, Leonard, Yuzawa, Satoshi, and Keasling, Jay D.

Abstract

Modular polyketide synthases (PKSs) are poly-merases that employ alpha-carboxyacyl-CoAs as extender substrates. This enzyme family contains several catalytic modules, where each module is responsible for a single round of polyketide chain extension. Although PKS modules typically use malonyl-CoA or methylmalonyl-CoA for chain elongation, many other malonyl-CoA analogues are used to diversify polyketide structures in nature. Previously, we developed a method to alter an extension substrate of a given module by exchanging an acyltransferase (AT) domain while maintaining protein folding. Here, we report in vitro polyketide biosynthesis by 13 PKSs (the wild-type PKS and 12 AT-exchanged PKSs with unusual ATs) and 14 extender substrates. Our similar to 200 in vitro reactions resulted in 13 structurally different polyketides, including several polyketides that have not been reported. In some cases, AT-exchanged PKSs produced target polyketides by >100-fold compared to the wild-type PKS. These data also indicate that most unusual AT domains do not incorporate malonyl-CoA and methylmalonyl-CoA but incorporate various rare extender substrates that are equal to in size or slightly larger than natural substrates. We developed a computational workflow to predict the approximate AT substrate range based on active site volumes to support the selection of ATs. These results greatly enhance our understanding of rare AT domains and demonstrate the benefit of using the proposed PKS engineering strategy to produce novel chemicals in vitro., QC 20230515

Published
2023
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23. Supplemental information MEP pathway products allosterically promote monomerization of deoxy-D-xylulose-5-phosphate synthase to feedback-regulate their supply

Author

Rodríguez-Concepción, Manuel [manuelrc@ibmcp.upv.es], Pérez-Gil, Jordi [jordi.perez@cragenomica.es], Di, Xueni, Ortega-Alarcón, David, Kakumanu, Ramu, Iglesias-Fernández, Javier, Díaz, Lucia, Baidoo, Edward E. K., Velázquez-Campoy, Adrián, Rodriguez-Concepcion, Manuel, Pérez-Gil, Jordi, Rodríguez-Concepción, Manuel [manuelrc@ibmcp.upv.es], Pérez-Gil, Jordi [jordi.perez@cragenomica.es], Di, Xueni, Ortega-Alarcón, David, Kakumanu, Ramu, Iglesias-Fernández, Javier, Díaz, Lucia, Baidoo, Edward E. K., Velázquez-Campoy, Adrián, Rodriguez-Concepcion, Manuel, and Pérez-Gil, Jordi

Published
2023

24. MEP pathway products allosterically promote monomerization of deoxy-D-xylulose-5-phosphate synthase to feedback-regulate their supply

Author

Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), European Commission, Ministerio de Economía y Competitividad (España), Consejo Superior de Investigaciones Científicas (España), Generalitat Valenciana, Department of Energy (US), China Scholarship Council, Rodríguez-Concepción, Manuel [0000-0002-1280-2305], Di, Xueni, Ortega-Alarcón, David, Kakumanu, Ramu, Iglesias-Fernández, Javier, Díaz, Lucia, Baidoo, Edward E. K., Velázquez-Campoy, Adrián, Rodriguez-Concepcion, Manuel, Pérez-Gil, Jordi, Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), European Commission, Ministerio de Economía y Competitividad (España), Consejo Superior de Investigaciones Científicas (España), Generalitat Valenciana, Department of Energy (US), China Scholarship Council, Rodríguez-Concepción, Manuel [0000-0002-1280-2305], Di, Xueni, Ortega-Alarcón, David, Kakumanu, Ramu, Iglesias-Fernández, Javier, Díaz, Lucia, Baidoo, Edward E. K., Velázquez-Campoy, Adrián, Rodriguez-Concepcion, Manuel, and Pérez-Gil, Jordi

Abstract

Isoprenoids are a very large and diverse family of metabolites required by all living organisms. All isoprenoids derive from the double-bond isomers isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP), which are produced by the methylerythritol 4-phosphate (MEP) pathway in bacteria and plant plastids. It has been reported that IPP and DMAPP feedback-regulate the activity of deoxyxylulose 5-phosphate synthase (DXS), a dimeric enzyme that catalyzes the main flux-controlling step of the MEP pathway. Here we provide experimental insights into the underlying mechanism. Isothermal titration calorimetry and dynamic light scattering approaches showed that IPP and DMAPP can allosterically bind to DXS in vitro, causing a size shift. In silico ligand binding site analysis and docking calculations identified a potential allosteric site in the contact region between the two monomers of the active DXS dimer. Modulation of IPP and DMAPP contents in vivo followed by immunoblot analyses confirmed that high IPP/DMAPP levels resulted in monomerization and eventual aggregation of the enzyme in bacterial and plant cells. Loss of the enzymatically active dimeric conformation allows a fast and reversible reduction of DXS activity in response to a sudden increase or decrease in IPP/DMAPP supply, whereas aggregation and subsequent removal of monomers that would otherwise be available for dimerization appears to be a more drastic response in the case of persistent IPP/DMAPP overabundance (e.g., by a blockage in their conversion to downstream isoprenoids). Our results represent an important step toward understanding the regulation of the MEP pathway and rational design of biotechnological endeavors aimed at increasing isoprenoid contents in microbial and plant systems.

Published
2023

28. Additional file 1 of Evaluation of engineered low-lignin poplar for conversion into advanced bioproducts

Author

Lin, Chien-Yuan, Geiselman, Gina M., Liu, Di, Magurudeniya, Harsha D., Rodriguez, Alberto, Chen, Yi-Chun, Pidatala, Venkataramana, Unda, Faride, Amer, Bashar, Baidoo, Edward E. K., Mansfield, Shawn D., Simmons, Blake A., Singh, Seema, Scheller, Henrik V., Gladden, John M., and Eudes, Aymerick

Abstract

Additional file 1: Table S1. Primers used for RT-PCR. Figure S1. The QsuB engineering strategy. Figure S2. Metabolic routes for microbial conversion of fermentable sugars from lignocellulosic hydrolysates into sesquiterpenes (epi-isozizaene and α-bisabolene) and fatty alcohols. Figure S3. Propagation and characterization of WT and QsuB poplar lines. Figure S4. Correlation between lignin content and DHB content in WT and QsuB biomass. Figure S5. Optical density of R. toruloides cultures after cultivation on large-scale poplar hydrolysates.

Published
2023
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30. Correction to “Expression of Dehydroshikimate Dehydratase in Sorghum Improves Biomass Yield, Accumulation of Protocatechuate, and Biorefinery Economics”

Author

Tian, Yang, primary, Yang, Minliang, additional, Lin, Chien-Yuan, additional, Park, Joon-Hyun, additional, Wu, Chuan-Yin, additional, Kakumanu, Ramu, additional, De Ben, Christopher M., additional, Dalton, Jutta, additional, Vuu, Khanh M., additional, Shih, Patrick M., additional, Baidoo, Edward E. K., additional, Temple, Stephen, additional, Putnam, Daniel H., additional, Scheller, Henrik V., additional, Scown, Corinne D., additional, and Eudes, Aymerick, additional

Published
2022
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34. Overexpression of a rice BAHD acyltransferase gene in switchgrass (Panicum virgatum L.) enhances saccharification

Author

Li, Guotian, Jones, Kyle C., Eudes, Aymerick, Pidatala, Venkataramana R., Sun, Jian, Xu, Feng, Zhang, Chengcheng, Wei, Tong, Jain, Rashmi, Birdseye, Devon, Canlas, Patrick E., Baidoo, Edward E. K., Duong, Phat Q., Sharma, Manoj K., Singh, Seema, Ruan, Deling, Keasling, Jay D., Mortimer, Jenny C., Loqué, Dominique, Bartley, Laura E., Scheller, Henrik V., and Ronald, Pamela C.

Published
2018
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36. Expression of Dehydroshikimate Dehydratase in Sorghum Improves Biomass Yield, Accumulation of Protocatechuate, and Biorefinery Economics

Author

Tian, Yang, primary, Yang, Minliang, additional, Lin, Chien-Yuan, additional, Park, Joon-Hyun, additional, Wu, Chuan-Yin, additional, Kakumanu, Ramu, additional, De Ben, Christopher M., additional, Dalton, Jutta, additional, Vuu, Khanh M., additional, Shih, Patrick M., additional, Baidoo, Edward E. K., additional, Temple, Stephen, additional, Putnam, Daniel H., additional, Scheller, Henrik V., additional, Scown, Corinne D., additional, and Eudes, Aymerick, additional

Published
2022
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37. Nitrogen Metabolism in Pseudomonas putida: Functional Analysis Using Random Barcode Transposon Sequencing

Author

Schmidt, Matthias, primary, Pearson, Allison N., additional, Incha, Matthew R., additional, Thompson, Mitchell G., additional, Baidoo, Edward E. K., additional, Kakumanu, Ramu, additional, Mukhopadhyay, Aindrila, additional, Shih, Patrick M., additional, Deutschbauer, Adam M., additional, Blank, Lars M., additional, and Keasling, Jay D., additional

Published
2022
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41. Nitrogen metabolism in Pseudomonas putida: functional analysis using random barcode transposon sequencing

Author

Schmidt, Matthias, primary, Pearson, Allison N., additional, Incha, Matthew R., additional, Thompson, Mitchell G., additional, Baidoo, Edward E. K., additional, Kakumanu, Ramu, additional, Mukhopadhyay, Aindrilla, additional, Shih, Patrick M., additional, Deutschbauer, Adam M., additional, Blank, Lars M., additional, and Keasling, Jay D., additional

Published
2021
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43. Engineering sorghum for higher 4-hydroxybenzoic acid content

Author

Lin, Chien-Yuan, primary, Tian, Yang, additional, Nelson-Vasilchik, Kimberly, additional, Hague, Joel, additional, Kakumanu, Ramu, additional, Lee, Mi Yeon, additional, Trinh, Jessica, additional, Northen, Trent R., additional, Baidoo, Edward E. K., additional, Kausch, Albert P., additional, Scheller, Henrik V., additional, and Eudes, Aymerick, additional

Published
2021
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44. Additional file 1 of Expression of a bacterial 3-dehydroshikimate dehydratase (QsuB) reduces lignin and improves biomass saccharification efficiency in switchgrass (Panicum virgatum L.)

Author

Zhangying Hao, Yogiswara, Sasha, Wei, Tong, Benites, Veronica Teixeira, Anagh Sinha, Wang, George, Baidoo, Edward E. K., Ronald, Pamela C., Scheller, Henrik V., Loqué, Dominique, and Eudes, Aymerick

Abstract

Additional file 1: Figure S1. Representative pictures showing GUS activities in various tiller sections of switchgrass lines harboring the pShOMT::GUS construct. GUS expression is specifically observed in stem nodes. Scale: White bars = 2 mm, black bar = 400 μ m. N: node; IN: internode, IS: internode transverse section.

Published
2021
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45. Additional file 2 of Expression of a bacterial 3-dehydroshikimate dehydratase (QsuB) reduces lignin and improves biomass saccharification efficiency in switchgrass (Panicum virgatum L.)

Author

Zhangying Hao, Yogiswara, Sasha, Wei, Tong, Benites, Veronica Teixeira, Anagh Sinha, Wang, George, Baidoo, Edward E. K., Ronald, Pamela C., Scheller, Henrik V., Loqué, Dominique, and Eudes, Aymerick

Abstract

Additional file 2: Figure S2. Characterization of a switchgrass line harboring the pZmCesa10::QsuB construct. (A) Representative pictures showing GUS activities in various tiller sections of switchgrass lines harboring the pZmCesa10::GUS construct. GUS expression is mostly observed in internodes, especially in developing vascular bundles (red arrows). Scale: White bars = 2 mm, black bar = 400 μ m. N: node; IN: internode, IS: internode transverse section. (B) Detection of the QsuB gene by PCR in line pZmCesa10::QsuB-5. (C) Detection of QsuB transcripts by RT-qPCR. QsuB expression levels relative to that of PvUBQ6 are shown. Values are means ±SD of two biological replicates (n = 2). (D) Protocatechuate (PCA) content measured in the biomass of the switchgrass line pZmCesa10::QsuB-5. A line containing the pZmCesa10::GUS construct was used as control. Values are means ±SE of three biological replicates (n = 3). Asterisks indicate a significant difference from the control using the unpaired Student’s t-test (*P

Published
2021
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46. Additional file 4 of Expression of a bacterial 3-dehydroshikimate dehydratase (QsuB) reduces lignin and improves biomass saccharification efficiency in switchgrass (Panicum virgatum L.)

Author

Zhangying Hao, Yogiswara, Sasha, Wei, Tong, Benites, Veronica Teixeira, Anagh Sinha, Wang, George, Baidoo, Edward E. K., Ronald, Pamela C., Scheller, Henrik V., Loqué, Dominique, and Eudes, Aymerick

Abstract

Additional file 4: Figure S3. Full length unprocessed images of PCR gels used for Figs. 2a and S2B. Note that the seven transformants obtained with the pZmUbi-1::QsuB construct were all false positives and did not contain the QsuB gene (see purple rectangle on the PCR gel).

Published
2021
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47. Additional file 3 of Expression of a bacterial 3-dehydroshikimate dehydratase (QsuB) reduces lignin and improves biomass saccharification efficiency in switchgrass (Panicum virgatum L.)

Author

Zhangying Hao, Yogiswara, Sasha, Wei, Tong, Benites, Veronica Teixeira, Anagh Sinha, Wang, George, Baidoo, Edward E. K., Ronald, Pamela C., Scheller, Henrik V., Loqué, Dominique, and Eudes, Aymerick

Abstract

Additional file 3: Table S1. Oligonucleotides used in the study.

Published
2021
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48. Additional file 1 of Further engineering of R. toruloides for the production of terpenes from lignocellulosic biomass

Author

Kirby, James, Geiselman, Gina M., Yaegashi, Junko, Joonhoon Kim, Zhuang, Xun, Tran-Gyamfi, Mary Bao, Jan-Philip Prahl, Sundstrom, Eric R., Yuqian Gao, Munoz, Nathalie, Burnum-Johnson, Kristin E., Benites, Veronica T., Baidoo, Edward E. K., Fuhrmann, Anna, Seibel, Katharina, Bobbie-Jo M. Webb-Robertson, Zucker, Jeremy, Nicora, Carrie D., Tanjore, Deepti, Magnuson, Jon K., Skerker, Jeffrey M., and Gladden, John M.

Subjects
Data_FILES
Abstract

Additional file 1: Supplemental Figures and Tables.

Published
2021
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49. Additional file 1 of Overexpression of the rice BAHD acyltransferase AT10 increases xylan-bound p-coumarate and reduces lignin in Sorghum bicolor

Author

Tian, Yang, Lin, Chien-Yuan, Park, Joon-Hyun, Wu, Chuan-Yin, Kakumanu, Ramu, Pidatala, Venkataramana R., Vuu, Khanh M., Rodriguez, Alberto, Shih, Patrick M., Baidoo, Edward E. K., Temple, Stephen, Simmons, Blake A., Gladden, John M., Scheller, Henrik V., and Eudes, Aymerick

Abstract

Additional file 1: Figure S1. Representative liquid chromatography���mass spectrometry (LC���MS) chromatograms obtained from analysis of TFA hydrolysates from CWR of wildtype (orange traces) and pSbUbi:AT10 (blue traces) sorghum stems. Peaks corresponding to pCA-Ara (a) and FA-Ara (b) display major ions at m/z 295 and 325, respectively. Electrospray ionization collision-induced dissociation tandem mass (ESI���CID���MS/MS) spectra of pCA-Ara (c) and FA-Ara (d) are shown. Ions at m/z 265 and 235 correspond to the 0,2A1 and 0,3A1 arabinose cross-ring cleavage ions as previously reported (Qu��m��ner and Ralet 2004). In addition, ions from deprotonated p-coumarate (m/z 163) and ferulate (m/z 193) are observed. Figure S2. Representative LC���MS chromatogram obtained from analysis of alkaline hydrolysates from CWR of wildtype sorghum stems. Seven peaks with ions at m/z 385 corresponding to diferulate isomers are observed. ESI���MS spectra from each peak are shown (bottom panels). Tentative diferulate chemical structures are based on the seven compounds previously identified in alkaline hydrolysates obtained from switchgrass and maize stalk cell walls (Ralph et al. 1994; Marita et al. 2003). Table S1. Growth parameters of wildtype and pSbUbi:AT10 lines. Values in brackets are the SE from five biological replicates (n = 5). Table S2. Characteristics and relative molar abundances (%) of guaiacyl (G) and syringyl (S) lignin-specific pyrolysis products released from CWR of wildtype (WT) and pSbUbi:AT10 lines. Values in brackets are the SE from four biological replicates (n = 4). Table S3. List of primers used in this study. Table S4. List of plasmids used in this study.

Published
2021
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