Search

Your search keyword '"Bahnassawy, Lamiaa"' showing total 17 results
Start Over Author "Bahnassawy, Lamiaa"
17 results on '"Bahnassawy, Lamiaa"'

1. Establishment of a high-content imaging assay for tau aggregation in hiPSC-derived neurons differentiated from two protocols to routinely evaluate compounds and genetic perturbations

Author

Bahnassawy, Lamiaa, Nicolaisen, Nathalie, Untucht, Christopher, Mielich-Süss, Benjamin, Reinhardt, Lydia, Ried, Janina S., Morawe, Martina P., Geist, Daniela, Finck, Anja, Käfer, Elke, Korffmann, Jürgen, Townsend, Matthew, Ravikumar, Brinda, Lakics, Viktor, Cik, Miroslav, and Reinhardt, Peter

Published
2024
Full Text
View/download PDF

2. Functional characterization of a single nucleotide polymorphism associated with Alzheimer’s disease in a hiPSC-based neuron model

Author

Stolzenburg, Lindsay R., primary, Esmaeeli, Sahar, additional, Kulkarni, Ameya S., additional, Murphy, Erin, additional, Kwon, Taekyung, additional, Preiss, Christina, additional, Bahnassawy, Lamiaa, additional, Stender, Joshua D., additional, Manos, Justine D., additional, Reinhardt, Peter, additional, Rahimov, Fedik, additional, Waring, Jeffrey F., additional, and Ramathal, Cyril Y., additional

Published
2023
Full Text
View/download PDF

4. Generation of a set of isogenic iPSC lines carrying all APOE genetic variants (epsilon 2/epsilon 3/epsilon 4) and knock-out for the study of APOE biology in health and disease

Author

Schmid, Benjamin, Holst, Bjorn, Clausen, Christian, Bahnassawy, Lamiaa, Reinhardt, Peter, Bakker, Margot H. M., Diaz-Guerra, Eva, Vicario, Carlos, Martino-Adami, Pamela, V, Thoenes, Michaela, Ramirez, Alfredo, Flissbach, Klaus, Grezella, Clara, Bruestle, Oliver, Peitz, Michael, Ebneth, Andreas, Cabrera-Socorro, Alfredo, Schmid, Benjamin, Holst, Bjorn, Clausen, Christian, Bahnassawy, Lamiaa, Reinhardt, Peter, Bakker, Margot H. M., Diaz-Guerra, Eva, Vicario, Carlos, Martino-Adami, Pamela, V, Thoenes, Michaela, Ramirez, Alfredo, Flissbach, Klaus, Grezella, Clara, Bruestle, Oliver, Peitz, Michael, Ebneth, Andreas, and Cabrera-Socorro, Alfredo

Abstract

APOE genotype is the strongest genetic risk factor for Alzheimer's Disease (AD). The low degree of homology between mouse and human APOE is a concerning issue in preclinical models currently used to study the role of this gene in AD pathophysiology. A key objective of ADAPTED (Alzheimer's Disease Apolipoprotein Pathology for Treatment Elucidation and Development) project was to generate in vitro models that better recapitulate human APOE biology. We describe a new set of induced pluripotent stem cells (iPSC) lines carrying common APOE variants (epsilon 2,epsilon 3, and epsilon 3/epsilon 4) and a knock-out isogenic to the parental APOE epsilon 4/epsilon 4 line (UKBi011-A).

Published
2021

5. Generation of a set of isogenic iPSC lines carrying all APOE genetic variants (Ɛ2/Ɛ3/Ɛ4) and knock-out for the study of APOE biology in health and disease

Author

Schmid, Benjamin, primary, Holst, Bjørn, additional, Clausen, Christian, additional, Bahnassawy, Lamiaa, additional, Reinhardt, Peter, additional, Bakker, Margot H.M., additional, Díaz-Guerra, Eva, additional, Vicario, Carlos, additional, Martino-Adami, Pamela V., additional, Thoenes, Michaela, additional, Ramirez, Alfredo, additional, Fliessbach, Klaus, additional, Grezella, Clara, additional, Brüstle, Oliver, additional, Peitz, Michael, additional, Ebneth, Andreas, additional, and Cabrera-Socorro, Alfredo, additional

Published
2021
Full Text
View/download PDF

6. Characterization of transcriptional and proteomics changes in brain cells derived from isogenic hiPSCs reveals cell type–and genotype‐specific mechanisms modified by APOE

Author

Ried, Janina S., primary, Curado, Marco Rocha, additional, Sáez, María Eugenia, additional, Bahnassawy, Lamiaa, additional, Lee, Heyne, additional, Reinhardt, Peter, additional, Mohler, Eric G., additional, Madrid, Laura, additional, Socorro, Alfredo Cabrera, additional, Grezella, Clara, additional, Nicolas, Armel, additional, Rao, Francesco, additional, Ramaswamy, Gayathri, additional, and Bakker, Margot H.M., additional

Published
2020
Full Text
View/download PDF

7. Proteomic, metabolomic, and transcriptomic examination of APOE transgenic mice

Author

Mohler, Eric G., primary, Ried, Janina S., additional, Bahnassawy, Lamiaa, additional, Reinhardt, Peter, additional, Curado, Marco Rocha, additional, Bakker, Margot H.M., additional, Nicolas, Armel, additional, Rao, Francesco, additional, Kohler, Isabelle, additional, Hankemeier, Thomas, additional, Ramaswamy, Gayathri, additional, and Sáez, María Eugenia, additional

Published
2020
Full Text
View/download PDF

8. Multilayer OMICS characterization of APOE‐modulated isogenic hiPSCs elucidates cell‐type–specific mechanisms modified by APOE: A study of the IMI ADAPTED Consortium

Author

Ried, Janina S., primary, Curado, Marco Rocha, additional, Sáez, María Eugenia, additional, Bahnassawy, Lamiaa, additional, Lee, Heyne, additional, Reinhardt, Peter, additional, Mohler, Eric G., additional, Madrid, Laura, additional, Socorro, Alfredo Cabrera, additional, Grezella, Clara, additional, Nicolas, Armel, additional, Rao, Francesco, additional, Ramaswamy, Gayathri, additional, and Bakker, Margot H.M., additional

Published
2020
Full Text
View/download PDF

9. Identification of APOE phenotypes in microglia and neurons derived from isogenic hips cells: A study of the IMI ADAPTED consortium

Author

Bahnassawy, Lamiaa, primary, Buchheit, Johanna, additional, Curado, Marco Rocha, additional, Ried, Janina S., additional, Sáez, María Eugenia, additional, Nicolas, Armel, additional, Rao, Francesco, additional, Kaplan, Charlotte, additional, Mohler, Eric G., additional, Lee, Heyne, additional, Untucht, Christopher, additional, Korffmann, Juergen, additional, Lakics, Viktor, additional, Cik, Miroslav, additional, Grezella, Clara, additional, Brustle, Oliver, additional, Peitz, Michael, additional, Mañes, Santos, additional, Ramaswamy, Gayathri, additional, Socorro, Alfredo Cabrera, additional, Bakker, Margot H.M., additional, and Reinhardt, Peter, additional

Published
2020
Full Text
View/download PDF

10. Modelling AD‐relevant pathophysiology in neurons, astrocytes and microglia from two complete sets of isogenic iPSC lines generated by the ADAPTED project

Author

Socorro, Alfredo Cabrera, primary, Schmid, Benjamin, additional, Clausen, Christian, additional, Holst, Bjørn, additional, Peitz, Michael, additional, Grezella, Clara, additional, Brustle, Oliver, additional, Bahnassawy, Lamiaa, additional, Bennett, Keiryn, additional, Ried, Janina S., additional, Sáez, María Eugenia, additional, Ramaswamy, Gayathri, additional, Ruiz, Agustin, additional, Bakker, Margot H.M., additional, Vicario, Carlos, additional, Lourenco, Teresa Silva, additional, and Reinhardt, Peter, additional

Published
2020
Full Text
View/download PDF

11. Fragment-Based Discovery of an Apolipoprotein E4 (apoE4) Stabilizer

Author

Petros, Andrew M., primary, Korepanova, Alla, additional, Jakob, Clarissa G., additional, Qiu, Wei, additional, Panchal, Sanjay C., additional, Wang, Jie, additional, Dietrich, Justin D., additional, Brewer, Jason T., additional, Pohlki, Frauke, additional, Kling, Andreas, additional, Wilcox, Kyle, additional, Lakics, Viktor, additional, Bahnassawy, Lamiaa, additional, Reinhardt, Peter, additional, Partha, Sarathy Karunan, additional, Bodelle, Pierre M., additional, Lake, Marc, additional, Charych, Erik I., additional, Stoll, Vincent S., additional, Sun, Chaohong, additional, and Mohler, Eric G., additional

Published
2019
Full Text
View/download PDF

12. Investigating the Role of APOE Genotype in Human Microglia.

Author

Ulm, Brittany, Amin, Najaf, Bahnassawy, Lamiaa, Reinhardt, Peter, Curado, Marco Rocha, Peitz, Michael, Fliessbach, Klaus, Ramirez, Alfredo, Ruiz, Agustin, Ried, Janina S, Bakker, Margot H.M., and Van Duijn, Cornelia M

Abstract

Background: Although the pivotal role of apolipoprotein E () in AD has been recognized for three decades, its function in different brain cells is far from understood. Knockout studies of the APOE gene may shed light of the function of ApoE. The aim of the present study was to unravel its function in induced pluripotent stem cell (iPSC) derived microglia. Method: We studied two human iPSC‐derived microglia cell cultures, one derived from a healthy control (Bioni010‐C – endogenous genotype apolipoprotein E34 (APOE34)) and one from an AD patient (UKBi011‐A – endogenous genotype APOE44) and their edited isogenic cell‐lines (for UKBi011‐A, APOE22 and APOE33 and for Bioni010‐C, hemizygous due to gene editing, APOE2‐ and APOE3‐ (32971461, 29753274)). Additionally, APOE KO lines were generated from both backgrounds. We performed whole genome transcriptomic (IlluminaTruSequencing) and proteomic (TMT‐labelled Nano‐LC peptidomic MS) analysis (n = six per genotype). For transcriptomic data, log2‐normalized intensities were analysed with (in R); there were 13115 unique transcripts. For proteomics analysis, after global normalization, the data were locally normalized (by protein), the limma package was used to calculate FDR‐corrected p‐values (1% cut off); there were 3975 (Bioni010 samples) and 4349 (UKBi011 samples) proteins. In the quality control analysis for the proteomics, we excluded one outlier of the six per line based on principle component/Euclidean distance analysis. We extracted pairwise comparisons to APOE KO in transcriptomic (|logFC|>0.5, FDR <0.05) and proteomic (|logFC|>0 and FDR <0.05) data. Result: Twenty transcripts (including APOE) were concordantly differentially expressed across all comparisons between APOE‐expressing and KO. These are broadly involved in processes related to mitochondrial function/organization, apoptosis, transcriptional regulation, immune functions, extracellular matrix, and energy metabolism among others. Seven proteins were concordantly differentially expressed across all pairwise comparisons with APOE KO, involved in extracellular matrix, immune signalling, energy metabolism, and cytoskeletal structure. Conclusion: Our study finds APOE knockout leads to a number of changes related to immune, mitochondrial, and extracellular matrix genes, suggesting that APOE in human iPSC‐derived microglia cells influences immune and mitochondrial functions. [ABSTRACT FROM AUTHOR]

Published
2023
Full Text
View/download PDF

16. TRIM32-dependent transcription in adult neural progenitor cells regulates neuronal differentiation

Author

Luxembourg Centre for Systems Biomedicine (LCSB): Developmental and Cellular Biology (Schwamborn Group) [research center], Hillje, Anna-Lena, Pavlou, Maria Angeliki, Beckmann, Elisabeth, Worlitzer, Maik, Bahnassawy, Lamiaa, Lewejohann, Lars, Palm, Thomas, Schwamborn, Jens Christian, Luxembourg Centre for Systems Biomedicine (LCSB): Developmental and Cellular Biology (Schwamborn Group) [research center], Hillje, Anna-Lena, Pavlou, Maria Angeliki, Beckmann, Elisabeth, Worlitzer, Maik, Bahnassawy, Lamiaa, Lewejohann, Lars, Palm, Thomas, and Schwamborn, Jens Christian

Published
2013
Full Text
View/download PDF

17. Generating Human iPSC-Derived Astrocytes with Chemically Defined Medium for In Vitro Disease Modeling.

Author

Janssen K, Bahnassawy L, Kiefer C, Korffmann J, Terstappen GC, Lakics V, Cik M, and Reinhardt P

Subjects
Cell Culture Techniques, Cells, Cultured, Culture Media, Humans, Induced Pluripotent Stem Cells drug effects, Models, Biological, Neural Stem Cells drug effects, Neurodegenerative Diseases, Neurogenesis drug effects, Astrocytes cytology, Cell Differentiation drug effects, Induced Pluripotent Stem Cells cytology, Neural Stem Cells cytology
Abstract

To better understand and model neurological, in particular neurodegenerative diseases, human induced pluripotent stem cells (hiPSCs) offer a great source for generation of neural cells. We provide a protocol for the differentiation of hiPSc-derived astrocytes in vitro. This protocol not only is chemically defined, that is, it does not use serum, but also allows for the expansion of astrocyte progenitor cells and mature astrocytes. Large batches of hiPSc-derived astrocytes can be stored and used for defined in vitro disease models.

Published
2019
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results