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There has been a concentrated attempt to introduce bacteria with oxalate degrading activity into the mammalian gut microbiota to enhance ecosystem function towards more effective oxalate degradation and prevent diseases. A few reports have studied Lactic acid bacteria (LAB) as promising intestinal oxalate degrading probiotic to treat kidney stone disease. In the present study, a total of 495 LAB isolates from different dairy products samples were primary characterized. Eighty-eight of the isolates belonged to genus Lactobacillus and were screened for their ability to degrade oxalate. Twenty-three of isolated lactobacilli showed oxalate degradation ability. According to the diameter of clear zone and the viable count, ten isolates were the most potent isolates for oxalate degradation. After the genotypic identification using 16s rDNA sequencing for these potent isolates, only seven strains were belonging to genus Lactobacillus spp. Lactobacillus fermentum (5 strains) and Lactobacillus gastricus (2 strains). These isolates were assessed for potential probiotic properties in vitro for further in vivo assessment. In conclusion, they exhibited good probiotic characters that suggested them to serve as good probiotic candidates for managing hyperoxaluria.

Strains of potentially probiotic lactobacilli, propionibacteria, leuconostoc, lactococcus, enterococcus, and pediococcus, were tested for their ability to convert linoleic acid to conjugated linoleic acid (CLA). Growth and CLA production were followed during incubation for 48 h in reconstituted skim milk containing 0.2% lipolysed sesame oil. Lactococcus lactis subsp. lactis biovar diacetylactis and Leuconostoc mesenteroides subsp. mesenteroides gave the highest CLA production. Also, the effect of lipolysed oil concentration on the growth and CLA production of six strains were studied in medium containing 0.0―1% lipolysed oil. Leuconostoc mesenteroides subsp. mesenteroides and Lac. lactis subsp. lactis biovar diacetylactis gave maximum dienes in medium containing 0.6% and 0.8% lipolysed oil respectively.