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In light of the escalating global concern regarding adaptation and resilience to elevated temperatures due to climate change, this experiment was designed to assess the thermo-physiological attributes of two native sheep breeds (Najdi and Naimi) and to delineate potential genetic factors conferring heat tolerance amidst acute exposure to elevated ambient temperatures. Meteorological and thermo-physiological parameters were scrutinized at distinct intervals (0 min, 30 min, 120 min, 24 hr, and 48 hr), alongside the analysis of heat-responsive gene expression at 0 min, 30 min, and 120 min, following the exposure of nine healthy male lambs from each breed (mean body weight: 25 kg; age: 4 months) to a bio-meteorologically-simulated environment, maintaining an average ambient temperature of 45 °C (approximately 93 units in the temperature-humidity index). In addition, blood samples were collected from each lamb, with total RNA isolated and purity assessed, followed by qRTPCR analysis of 16 heat stress candidate genes using validated primers and standardized thermocycling protocols, including controls to ensure accuracy. Data were analyzed using statistical methods, including PROC GLM and PROC MEANS in SAS, one-way ANOVA, and pairwise differences with the LSD test for significance, while gene expression differences were calculated using the comparative Ct method and 2 (-Ct) for relative quantification. The findings elucidate that the Najdi breed manifests heightened thermotolerance relative to the Naimi breed, as evidenced by diminished indicators of heat stress, encompassing skin temperature, respiratory rate, packed cell volume, adaptability coefficient, serum total protein, glucose levels, and triiodothyronine concentration. Moreover, analysis of gene expression patterns revealed widespread activation of heat stress-responsive genes in both breeds under thermal stress conditions; however, Najdi lambs consistently exhibited elevated expression levels of these genes compared to their Naimi counterparts. Notably, genes including HSP90AB1, HSPB6, HSF1, STIP1, HSP60, HSP90, and HSPB1 demonstrated particularly pronounced upregulation in Najdi lambs. In conclusion, the integrative thermo-physiological and molecular profiling highlights the superior thermotolerance and evolutionary adaptation of the Najdi breed to the hot climate of the KSA, in contrast to the Naimi breed. [ABSTRACT FROM AUTHOR]

Some basic questions regarding sheep's ability to tolerate different levels of water intake (WI), especially when fed on a pelleted-complete diet (PCD) and reared under comfortable climatic conditions, are still open. To investigate the direct influence of different levels of WI in sheep fed PCD and reared under such conditions on the lambs' physiological (performance, thermal, blood and urine) status, 24 healthy male growing Najdi lambs were exposed under thermo-neutral conditions to three levels of WI (100%, 67%, and 33% of their ad libitum WI or water requirement) for 6 weeks. Meteorological, production performance, thermo-physiological, as well as blood and urine biochemical measurements, were all determined. The obtained findings clearly substantiate that reducing the level of water intake (p<0.05) produced tangible effects in both 67-WI and 33-WI lamb groups. However, 67-WI lambs showed resilience to limited water availability by inducing proportional physiological responses in their production performances, body temperatures, blood metabolites, and renal function but within the homeostatic ranges similar (p>0.05) to 100-WI lambs. Results collectively signify that diminishing WI up to 33% in lambs (fed on PCD and reared under comfortable conditions) would ensure that water is adequately conserved under prevailing water scarcity and can be implemented without compromising their homeostatic functions. Such applied approaches can consequently have a pragmatic aspect through improving water management approaches required for PCD-based sheep production under water scarcity conditions, which is crucial to economical animal production and responsible animal stewardship. Feasibility studies are thereby highly recommended. [ABSTRACT FROM AUTHOR]

This work concerns an enlarged analysis of the problem of asymptotic compensation for a class of discrete linear distributed systems. We study the possibility of asymptotic compensation of a disturbance by bringing asymptotically the observation in a given tolerance zone 풞. Under convenient hypothesis, we show the existence and the unicity of the optimal control ensuring this compensation and we give its characterization [ABSTRACT FROM PUBLISHER]