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243 results on '"Bae, Seunghee"'

2. Potential anti-ageing effects of probiotic-derived conditioned media on human skin cells

Author

Hong Yoo Kyung, An Sungkwan, Lee Yun Hee, Yang Seung Ah, Yoon Yoo Kyung, Lee Joonil, Lee Gwasoo, Chung Myung Jun, and Bae Seunghee

Subjects
anti-ageing, skin cells, probiotics, conditioned media, protection, anti-pigmentation, Pharmaceutical industry, HD9665-9675
Abstract

In this study, the protective functions of bacteria-free conditioned media from Bifidobacterium and Lactobacillus species against ultraviolet radiation-induced skin ageing and associated cellular damage were investigated. The effects of ultraviolet radiation-induced reactive oxygen species production were suppressed by all conditioned media; particularly, the loss of cell viability and downregulation of collagen gene expression were significantly reversed by the conditioned media from B. longum and B. lactis. Further exa mination of potential anti-pigmentation effects revealed that the B. lactis-derived conditioned media significantly inhibited tyrosinase activity and alpha-melanocyte-stimulating hormone-induced melanin production in human epidermal melanocytes. Further, the conditioned media suppressed the phosphorylation of extracellular signal- related kinase, which functions as an upstream regulator of melanogenesis. Therefore, B. lactis-derived conditioned media can potentially protect against cellular damage involved in skin-ageing processes.

Published
2022
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20. Whitening and moisturizing enhancing effects of three‐dimensional human adipose‐derived mesenchymal stem cell‐conditioned medium‐containing cream.

Author

Kim, Kyung Hye, Lee, Sunray, and Bae, Seunghee

Subjects
STEM cell culture, COLD weather conditions, SKIN care, HYALURONIC acid
Abstract

Background: High‐functional cosmetic products combined with the concept of "treatment" cosmetics are being introduced to the market. Cosmetic products containing a skin‐derived microbiome, a three‐dimensional (3D) stem cell culture medium, and low‐molecular‐weight collagen are being introduced, and these products are leading the cosmeceutical market. We aimed to confirm the potential of a 3D stem cell culture medium‐containing cream as a skin‐whitening and moisturizing product. Aim: To determine the enhancing effects of a cream containing 3D adipose tissue‐derived mesenchymal stem cell‐conditioned media (3D ADMSC‐CM) on whitening and moisturization. Methods: The inhibitory activities of tyrosinase (TYR) and melanin were confirmed using 3D ADMSC‐CM. Furthermore, hyaluronic acid expression in 3D ADMSC‐CM was verified. The clinical efficacy of the cream containing 3D ADMSC‐CM was established by evaluating its antioxidant properties and effects on skin tone, radiance, freckles, and moisturization. Results: The use of 3D ADMSC‐CM suppressed the inhibitory effects of TYR and melanin by approximately 24% and 33%, respectively, and increased the expression of hyaluronic acid synthase. A significant difference was observed after 4 weeks of using 3D ADMSC‐CM in the skin antioxidant evaluation. After 2 and 4 weeks of use, skin tone and radiance increased and skin freckles decreased significantly. Under extremely cold and dry weather conditions, the use of the cream increased skin moisturization. Conclusions: The 3D ADMSC‐CM cream evaluated in an environment similar to the human body was found to enhance skin whitening and moisturization and can therefore be used in the skin care and cosmetic industries as a biocosmetic product. [ABSTRACT FROM AUTHOR]

Published
2023
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22. Development of a high-throughput screening system for identification of novel reagents regulating DNA damage in human dermal fibroblasts

Author

Bae Seunghee, An In-Sook, and An Sungkwan

Subjects
human dermal fibroblasts, dna damage, high-throughput screening, aging, Pharmaceutical industry, HD9665-9675
Abstract

Ultraviolet (UV) radiation is a major inducer of skin aging and accumulated exposure to UV radiation increases DNA damage in skin cells, including dermal fibroblasts. In the present study, we developed a novel DNA repair regulating material discovery (DREAM) system for the high-throughput screening and identification of putative materials regulating DNA repair in skin cells. First, we established a modified lentivirus expressing the luciferase and hypoxanthine phosphoribosyl transferase (HPRT) genes. Then, human dermal fibroblast WS-1 cells were infected with the modified lentivirus and selected with puromycin to establish cells that stably expressed luciferase and HPRT (DREAM-F cells). The first step in the DREAM protocol was a 96-well-based screening procedure, involving the analysis of cell viability and luciferase activity after pretreatment of DREAM-F cells with reagents of interest and post-treatment with UVB radiation, and vice versa. In the second step, we validated certain effective reagents identified in the first step by analyzing the cell cycle, evaluating cell death, and performing HPRT-DNA sequencing in DREAM-F cells treated with these reagents and UVB. This DREAM system is scalable and forms a time-saving high-throughput screening system for identifying novel anti-photoaging reagents regulating DNA damage in dermal fibroblasts.

Published
2015
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47. Notch Signaling in Hair Follicle Development

Author

Bae， Seunghee, Lee Ki Heon, and Jae Ho Lee

Subjects
0301 basic medicine, 03 medical and health sciences, 030104 developmental biology, Chemistry, General Medicine
Published
2017

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