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134 results on '"BOMBELLI, RAFFAELLA"'

1. Immunogenicity of anti-SARS-CoV-2 Comirnaty vaccine in patients with lymphomas and myeloma who underwent autologous stem cell transplantation

Author

Salvini, Marco, Maggi, Fabrizio, Damonte, Camilla, Mortara, Lorenzo, Bruno, Antonino, Mora, Barbara, Brociner, Marco, Mattarucchi, Roberta, Ingrassia, Alessia, Sirocchi, Davide, Bianchi, Benedetta, Agnoli, Stefania, Gallazzi, Matteo, Merli, Michele, Ferrario, Andrea, Bombelli, Raffaella, Barraco, Daniela, Baj, Andreina, Bertù, Lorenza, Grossi, Paolo A., and Passamonti, Francesco

Published
2022
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6. Parkinson’s disease patients have a complex phenotypic and functional Th1 bias: cross-sectional studies of CD4+ Th1/Th2/T17 and Treg in drug-naïve and drug-treated patients

Author

Kustrimovic, Natasa, Comi, Cristoforo, Magistrelli, Luca, Rasini, Emanuela, Legnaro, Massimiliano, Bombelli, Raffaella, Aleksic, Iva, Blandini, Fabio, Minafra, Brigida, Riboldazzi, Giulio, Sturchio, Andrea, Mauri, Marco, Bono, Giorgio, Marino, Franca, and Cosentino, Marco

Published
2018
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7. Immunogenicity and clinical efficacy of anti‐SARS‐CoV‐2 vaccination in patients with hematological malignancies: Results of a prospective cohort study of 365 patients

Author

Salvini, Marco, primary, Damonte, Camilla, additional, Mortara, Lorenzo, additional, Maggi, Fabrizio, additional, Bruno, Antonino, additional, Pellegrini, Giacomo, additional, Mora, Barbara, additional, Brociner, Marco, additional, Ingrassia, Alessia, additional, Mattarucchi, Roberta, additional, Bianchi, Benedetta, additional, Sirocchi, Davide, additional, Agnoli, Stefania, additional, Rumi, Elisa, additional, Merli, Michele, additional, Fossati, Alessandro, additional, Bassi, Susanna, additional, Bombelli, Raffaella, additional, Gallazzi, Matteo, additional, Borsani, Oscar, additional, Baj, Andreina, additional, Franchi, Matteo, additional, Grossi, Paolo A., additional, and Passamonti, Francesco, additional

Published
2022
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9. Immunogenicity of anti-SARS-CoV-2 Comirnaty vaccine in patients with lymphomas and myeloma who underwent autologous stem cell transplantation

Author

Salvini, Marco, primary, Maggi, Fabrizio, additional, Damonte, Camilla, additional, Mortara, Lorenzo, additional, Bruno, Antonino, additional, Mora, Barbara, additional, Brociner, Marco, additional, Mattarucchi, Roberta, additional, Ingrassia, Alessia, additional, Sirocchi, Davide, additional, Bianchi, Benedetta, additional, Agnoli, Stefania, additional, Gallazzi, Matteo, additional, Merli, Michele, additional, Ferrario, Andrea, additional, Bombelli, Raffaella, additional, Barraco, Daniela, additional, Baj, Andreina, additional, Bertù, Lorenza, additional, Grossi, Paolo A., additional, and Passamonti, Francesco, additional

Published
2021
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15. BRCA Methylation Testing Identifies a Subset of Ovarian Carcinomas without Germline Variants That Can Benefit from PARP Inhibitor

Author

Sahnane, Nora, primary, Carnevali, Ileana, additional, Formenti, Giorgio, additional, Casarin, Jvan, additional, Facchi, Sofia, additional, Bombelli, Raffaella, additional, Di Lauro, Eleonora, additional, Memoli, Domenico, additional, Salvati, Annamaria, additional, Rizzo, Francesca, additional, Sessa, Fausto, additional, and Tibiletti, Maria Grazia, additional

Published
2020
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21. Additional file 4: of Parkinson’s disease patients have a complex phenotypic and functional Th1 bias: cross-sectional studies of CD4+ Th1/Th2/T17 and Treg in drug-naïve and drug-treated patients

Author

Natasa Kustrimovic, Comi, Cristoforo, Magistrelli, Luca, Rasini, Emanuela, Legnaro, Massimiliano, Bombelli, Raffaella, Aleksic, Iva, Blandini, Fabio, Minafra, Brigida, Riboldazzi, Giulio, Sturchio, Andrea, Mauri, Marco, Bono, Giorgio, Marino, Franca, and Cosentino, Marco

Abstract

Table S3. Complete blood count in HS and PD patients. Data are means ± SD unless otherwise indicated. (DOCX 45 kb)

Published
2018
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22. Additional file 5: of Parkinson’s disease patients have a complex phenotypic and functional Th1 bias: cross-sectional studies of CD4+ Th1/Th2/T17 and Treg in drug-naïve and drug-treated patients

Author

Natasa Kustrimovic, Comi, Cristoforo, Magistrelli, Luca, Rasini, Emanuela, Legnaro, Massimiliano, Bombelli, Raffaella, Aleksic, Iva, Blandini, Fabio, Minafra, Brigida, Riboldazzi, Giulio, Sturchio, Andrea, Mauri, Marco, Bono, Giorgio, Marino, Franca, and Cosentino, Marco

Abstract

Table S4. Lymphocyte count, comparison between HS and PD patients. Data are means ± SD unless otherwise indicated. Differences are indicated only when statistically significant, and are reported as the mean differences (with 95% confidence interval) between the means. (DOCX 48 kb)

Published
2018
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23. Additional file 3: of Parkinsonâ s disease patients have a complex phenotypic and functional Th1 bias: cross-sectional studies of CD4+ Th1/Th2/T17 and Treg in drug-naĂŻve and drug-treated patients

Author

Natasa Kustrimovic, Comi, Cristoforo, Magistrelli, Luca, Rasini, Emanuela, Legnaro, Massimiliano, Bombelli, Raffaella, Aleksic, Iva, Blandini, Fabio, Minafra, Brigida, Riboldazzi, Giulio, Sturchio, Andrea, Mauri, Marco, Bono, Giorgio, Marino, Franca, and Cosentino, Marco

Abstract

Table S2. Real-time PCR conditions. (DOCX 24Â kb)

Published
2018
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24. Additional file 1: of Parkinsonâ s disease patients have a complex phenotypic and functional Th1 bias: cross-sectional studies of CD4+ Th1/Th2/T17 and Treg in drug-naĂŻve and drug-treated patients

Author

Natasa Kustrimovic, Comi, Cristoforo, Magistrelli, Luca, Rasini, Emanuela, Legnaro, Massimiliano, Bombelli, Raffaella, Aleksic, Iva, Blandini, Fabio, Minafra, Brigida, Riboldazzi, Giulio, Sturchio, Andrea, Mauri, Marco, Bono, Giorgio, Marino, Franca, and Cosentino, Marco

Abstract

Table S1. List of ab used in flow cytometric assays. (DOCX 19Â kb)

Published
2018
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25. Additional file 2: of Parkinson’s disease patients have a complex phenotypic and functional Th1 bias: cross-sectional studies of CD4+ Th1/Th2/T17 and Treg in drug-naïve and drug-treated patients

Author

Natasa Kustrimovic, Comi, Cristoforo, Magistrelli, Luca, Rasini, Emanuela, Legnaro, Massimiliano, Bombelli, Raffaella, Aleksic, Iva, Blandini, Fabio, Minafra, Brigida, Riboldazzi, Giulio, Sturchio, Andrea, Mauri, Marco, Bono, Giorgio, Marino, Franca, and Cosentino, Marco

Abstract

Figure S1. CD4+ Th cells and DR expression in whole blood. Gating strategy used to identify DR + CD4+ T lymphocytes. Figure S2. CD4+ Treg cells and DR expression in whole blood. Gating strategy used to identify DR + CD4+ Treg cells. Figure S3. DR expression on Th1 cells in HS and PD patients. Figure S4. DR expression on Th2 cells in HS and PD patients. Figure S5. DR expression on Th17 cells in HS and PD patients. Figure S6. DR expression on Th1/17 cells in HS and PD patients. Figure S7. CD4+ T cells in HS and PD patients enrolled in study #2. Figure S8. DR expression on Treg cells in HS and PD patients. Figure S9. DR expression on nTreg cells in HS and PD patients. Figure S10. DR expression on aTreg cells in HS and PD patients. Figure S11. Transcription factors mRNA levels in CD4+ T cells of HS and PD patients enrolled in study #2. (PPTX 10597 kb)

Published
2018
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30. Dataset of mRNA levels for dopaminergic receptors, adrenoceptors and tyrosine hydroxylase in lymphocytes from subjects with clinically isolated syndromes

Author

Cosentino, Marco, primary, Zaffaroni, Mauro, additional, Legnaro, Massimiliano, additional, Bombelli, Raffaella, additional, Schembri, Laura, additional, Baroncini, Damiano, additional, Bianchi, Anna, additional, Clerici, Raffaella, additional, Guidotti, Mario, additional, Banfi, Paola, additional, Bono, Giorgio, additional, and Marino, Franca, additional

Published
2016
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32. Dopaminergic Receptors on CD4+ T Naive and Memory Lymphocytes Correlate with Motor Impairment in Patients with Parkinson’s Disease

Author

Kustrimovic, Natasa, primary, Rasini, Emanuela, additional, Legnaro, Massimiliano, additional, Bombelli, Raffaella, additional, Aleksic, Iva, additional, Blandini, Fabio, additional, Comi, Cristoforo, additional, Mauri, Marco, additional, Minafra, Brigida, additional, Riboldazzi, Giulio, additional, Sanchez-Guajardo, Vanesa, additional, Marino, Franca, additional, and Cosentino, Marco, additional

Published
2016
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33. Increased expression of dopamine receptors in synovial fibroblasts from patients with rheumatoid arthritis: inhibitory effects of dopamine on interleukin-8 and interleukin-6

Author

Capellino, Silvia, Cosentino, Marco, Luini, Alessandra, Bombelli, Raffaella, Lowin, Torsten, Cutolo, Maurizio, Marino, Franca, and Straub, Rainer H.

Subjects
Dopamine Plasma Membrane Transport Proteins, Tissue Inhibitor of Metalloproteinase-2, Cultured, Tissue Inhibitor of Metalloproteinase-1, Interleukin-6, Arthritis, Cells, Dopamine, Interleukin-8, Synovial Membrane, Fibroblasts, Receptors, Dopamine, Arthritis, Rheumatoid, Rheumatoid, Receptors, Osteoarthritis, Humans, Matrix Metalloproteinase 3, Cells, Cultured
Abstract

Observations in both animal models of arthritis and patients with rheumatoid arthritis (RA) suggest a role for dopamine and its receptors in RA. Because synovial fibroblasts (SFs) contribute to inflammation and joint destruction in RA, the aim of this study was to investigate dopaminergic pathways in SFs obtained from patients with RA and, for comparison, in SFs from patients with osteoarthritis (OA) undergoing knee joint replacement surgery.The expression of all dopamine receptors (D1 -D5 ) and dopamine transporter was assessed by immunofluorescence and immunohistochemical staining. The levels of dopamine receptor and tyrosine hydroxylase messenger RNA were measured by real-time polymerase chain reaction. The intracellular content of dopamine, its precursor, and its main metabolites was assayed by high-performance liquid chromatography. The influence of dopamine on proinflammatory interleukin-6 (IL-6) and IL-8, matrix metalloproteinase 3, and tissue inhibitor of metalloproteinases 1 (TIMP-1) and TIMP-2 was studied in SFs.SFs possess an intrinsic dopaminergic system, including dopamine receptors, dopamine transporter, and tyrosine hydroxylase, and contain dopamine, its precursor, and its main metabolites. SFs from patients with RA, in comparison with those from patients with OA, showed increased expression of dopamine receptors D1 and D5 , and exogenous dopamine strongly inhibited the production of IL-8 in patients with RA.SFs from patients with RA and patients with OA show a dopaminergic phenotype. The expression of D1-like dopamine receptors was higher in RASFs, and this increased expression may lead to antiinflammatory effects, as demonstrated by the expression of IL-8. Studies in animal models and patients with RA are needed to assess the therapeutic potential of endogenous, local production of dopamine in synoviocytes.

Published
2013

34.  β2‐Adrenoceptors inhibit neutrophil extracellular traps in human polymorphonuclear leukocytes.

Author

Marino, Franca, Scanzano, Angela, Pulze, Laura, Pinoli, Monica, Rasini, Emanuela, Luini, Alessandra, Bombelli, Raffaella, Legnaro, Massimiliano, de Eguileor, Magda, and Cosentino, Marco

Subjects
BETA adrenoceptors, NEUTROPHILS, EXTRACELLULAR matrix, ADRENERGIC receptors, CELL migration, ANTI-inflammatory agents, REACTIVE oxygen species
Abstract

Abstract: This study tests the hypothesis that in isolated human polymorphonuclear leukocytes (PMN) adrenergic ligands can affect neutrophil extracellular trap (NET) formation. We have previously shown that, in PMN, adrenaline (A), through the activation of adrenergic receptors (AR), reduces stimulus‐dependent cell activation; we have, therefore, planned to investigate if AR are involved in NET production. PMN were obtained from venous blood of healthy subject. The ability of adrenergic ligands to affect reactive oxygen species (ROS) production, NET production, and cell migration was investigated in cells cultured under resting conditions or after activation with N‐formyl‐methionyl‐leucyl‐phenylalanine (fMLP), LPS, or IL‐8. Stimuli‐induced NET production measured as ROS, microscopic evaluation, and elastase production was reverted by A and this effect was blocked by the selective β2–AR antagonist ICI‐118,551. The stimulus‐induced ROS generation and migration was prevented by A and by isoprenaline (ISO), and these effects were counteracted only by ICI‐118,551 and not by the other two selective ligands for the β1 and β3–AR. Finally, the presence of the β–ARs on PMN was confirmed, by means of microscopy and flow cytometry. The data of the present study suggest that adrenergic compounds, through the interaction of mainly β2–AR, are able to affect neutrophil functions. These data are suggestive of a possible therapeutic role of β2–AR ligands (in addition to their classical use), promoting the possible therapeutic relevance of adrenergic system in the modulation of innate immunity proposing their possible use as anti‐inflammatory drugs. [ABSTRACT FROM AUTHOR]

Published
2018
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42. Dopaminergic Modulation of CD4+CD25high Regulatory T Lymphocytes in Multiple Sclerosis Patients during Interferon-β Therapy

Author

Cosentino, Marco, primary, Zaffaroni, Mauro, additional, Trojano, Maria, additional, Giorelli, Maurizio, additional, Pica, Carmela, additional, Rasini, Emanuela, additional, Bombelli, Raffaella, additional, Ferrari, Marco, additional, Ghezzi, Angelo, additional, Comi, Giancarlo, additional, Livrea, Paolo, additional, Lecchini, Sergio, additional, and Marino, Franca, additional

Published
2012
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44. Human CD4+CD25+ regulatory T cells selectively express tyrosine hydroxylase and contain endogenous catecholamines subserving an autocrine/paracrine inhibitory functional loop

Author

Cosentino, Marco, primary, Fietta, Anna Maria, additional, Ferrari, Marco, additional, Rasini, Emanuela, additional, Bombelli, Raffaella, additional, Carcano, Elena, additional, Saporiti, Federica, additional, Meloni, Federica, additional, Marino, Franca, additional, and Lecchini, Sergio, additional

Published
2006
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45. Adrenergic mechanisms in multiple sclerosis: the neuro–immune connection?

Author

Cosentino, Marco, primary, Zaffaroni, Mauro, additional, Giorelli, Maurizio, additional, Marino, Franca, additional, Ferrari, Marco, additional, Bombelli, Raffaella, additional, Rasini, Emanuela, additional, Trojano, Maria, additional, Lecchini, Sergio, additional, Livrea, Paolo, additional, Comi, Giancarlo, additional, and Frigo, Gianmario, additional

Published
2004
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49. Dopaminergic Modulation of Apoptosis in Human Peripheral Blood Mononuclear Cells: Possible Relevance for Parkinson's Disease.

Author

COLOMBO, CRISTINA, COSENTINO, MARCO, MARINO, FRANCA, RASINI, EMANUELA, OSSOLA, MARIA, BLANDINI, FABIO, MANGIAGALLI, ANNA, SAMUELE, ALBERTA, FERRARI, MARCO, BOMBELLI, RAFFAELLA, LECCHINI, SERGIO, NAPPI, GIUSEPPE, and FRIGO, GIANMARIO

Subjects
APOPTOSIS, NEURODEGENERATION, PARKINSON'S disease, CATECHOLAMINES, LEUCOCYTES, DOPAMINE antagonists, CHEMICAL inhibitors, GLUTATHIONE, REACTIVE oxygen species
Abstract

Dopamine (DA) modulates apoptosis in neuronal and non-neuronal cells, and dopaminergic pathways contribute to neurodegenerative disease. Human lymphocytes express dopaminergic recptors and DA transporters, and synthesize endogenous catecholamines, which may modulate apoptosis in these cells. In the present study, dopaminergic modulation of apoptosis was investigated in human peripheral blood mononuclear cells (PBMCs) obtained from healthy donors. Twenty-four-hour DA reduced at 0.1-5 × 10-6 M and enhanced at 1-5 × 10-4 M spontaneous apoptosis. DA 1 × 10-6 M was inhibited by the D1-like receptor antagonist SCH 23390 1 × 10-6 M, but not by the D2-like receptor antagonists domperidone 1 × 10-6 M or haloperidol 1 × 10-6 M, while the effect of DA 5 × 10-4 M was prevented by the antioxidants glutathione 5-10 mM or N-acetyl-L-cysteine 1-10 mM. Intracellular reactive oxygen species were respectively reduced and increased by 1-3 h incubation with DA 0.1-10 × 10-6 M and 1-5×10-4 M. Twenty-four-hour DA 1 × 10-6 M or 5 × 10-4 M had no effect on PBMC expression of Cu/Zn superoxide dismutase or Bcl-2; however, DA 5 × 10-4 M decreased caspase-3 activity. In human PBMCs, DA seems to promote apoptosis through oxidative mechanisms but may also result in cell rescue from apoptotic death possibly through activation of D1-like receptors. The dual effect of DA on human PBMCs closely resembles that on striatal neurons. Lymphocytes of patients with Parkinson's disease may show reduced DA content and impaired DA transporter immunoreactivity. Human PBMCs may thus represent a simple and readily accessible model to study DA-related mechanisms relevant for neurodegenerative disease. [ABSTRACT FROM AUTHOR]

Published
2004
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50. Human CD4+CD25+ regulatory T cells selectively express tyrosine hydroxylase and contain endogenous catecholamines subserving an autocrine/paracrine inhibitory functional loop

Author

Cosentino, Marco, Fietta, Anna Maria, Ferrari, Marco, Rasini, Emanuela, Bombelli, Raffaella, Carcano, Elena, Saporiti, Federica, Meloni, Federica, Marino, Franca, and Lecchini, Sergio

Abstract

CD4+CD25+ regulatory T lymphocytes (Tregs) are specialized T cells playing a key role in the control of immune homeostasis. Here, we show that human Tregs constitutively express tyrosine hydroxylase (TH, EC 1.14.16.2), the rate-limiting enzyme in the synthesis of catecholamines, and contain substantial amounts of dopamine, norepinephrine, and epinephrine, which are released upon treatment with reserpine. Catecholamine release results in reduced production of interleukin-10 and transforming growth factor-β by Tregs, and in down-regulation of Treg-dependent inhibition of effector T-lymphocyte (Teff) proliferation, which occurs without affecting the production of tumor necrosis factor-α or interferon-γ. Tregs and Teffs express on the cell membrane both D1-like and D2-like dopaminergic receptors to a similar extent (12%-29% of the cells). Catecholamine-dependent down-regulation of Tregs is, however, selectively reversed by pharmacological blockade of dopaminergic D1-like receptors, which in Tregs only (and not in Teffs) are also expressed at the level of mRNA and are functionally coupled to intracellular production of cAMP. These findings indicate that in human Tregs endogenous catecholamines subserve an autocrine/paracrine loop involving dopaminergic pathways and resulting in down-regulation of Treg function.

Published
2007
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