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117 p., Free-living amoebas have been isolated from habitats in common with mycobacteria including cold water for consumption, hot water systems in hospitals, contact lenses, among other habitats. The purpose of this study is to test the endosymbiotic relationship between free-living amoebas and Mycobacterium spp. Objective: Determining endosymbiosis of Mycobacterium spp in free-living amoebas in water samples from Cúcuta, 2019.Methods:The techniques used were membrane filtration and planting in nutritious agar for AVL isolation, lysis and Zielh-Neelsen coloration for the detection of BAAR and confirmation of the presence of these by planting in Ogawa Kudoh agar. Results: the high presence of free-living amoebas in the three samplings in all types of water (49%), indicating that both consumer water, rivers, swimming pool and residuals can possess such microorganisms. there is an endosymbiosis of Mycobacterium spp and aVLs of 30% of the collected samples, confirmed in Zielh Neelsen tying and microbiological culture compatible with Resistant Alcohol Acid Bacillus., Las amebas de vida libre (AVL) han sido aisladas de hábitats en común con las micobacterias incluyendo agua fría para el consumo, sistemas de agua caliente en hospitales, lentes de contacto, entre otros hábitats; El propósito de este estudio se basa en probar la relación endosimbiótica entre las amebas de vida libre y Mycobacterium spp. Objetivo: Determinar la endosimbiosis de Mycobacterium spp en amebas de vida libre en muestras de agua de Cúcuta, 2019. Métodos: Las técnicas empleadas fueron filtración por membrana y siembra en agar nutritivo para el aislamiento de AVL, lisis y coloración de Zielh-Neelsen para la detección de BAAR y confirmación de la presencia de éstos por siembra en agar Ogawa Kudoh Resultados: se demuestra la alta presencia de amebas de vida libre en los tres muestreos realizados en aguas de todo tipo (49%), lo cual indica que tanto aguas de consumo, ríos, piscina como residuales pueden poseer este tipo de microorganismos. Existe una endosimbiosis de Mycobacterium spp y las AVL en un 30% de las muestras recolectadas, confirmada en la tinción de Zielh Neelsen y cultivo microbiológico compatibles con Bacilos Acido Alcohol Resistentes., Pregrado, Bacteriólogo(a) y Laboratorista Clínico, INTRODUCCIÓN ................................................................................................... 23 1. PROBLEMA ....................................................................................................... 25 1.1 PLANTEAMIENTO DE PROBLEMA ................................................................ 25 1.2 FORMULACION DEL PROBLEMA .................................................................. 28 1.3 OBJETIVOS ..................................................................................................... 28 1.3.1 Objetivo General ........................................................................................... 28 1.3.2 Objetivos Específicos .................................................................................... 28 1.4. JUSTIFICACIÓN ............................................................................................. 28 2. MARCO REFERENCIAL.................................................................................... 30 2.1 ANTECEDENTES ............................................................................................ 30 2.2 MARCO TEÓRICO .......................................................................................... 34 2.2.1 EL AGUA ...................................................................................................... 34 2.2.2 Amebas de vida libre .................................................................................... 34 2.2.2 Mycobacterium spp ....................................................................................... 45 2.2.3. Endosimbiosis bacteriana ........................................................................... 54 2.3 MARCO CONCEPTUAL .................................................................................. 56 2.4 Marco legal ...................................................................................................... 56 2.5 MARCO CONTEXTUAL .................................................................................. 58 2.6 SISTEMA DE HIPÓTESIS ............................................................................... 59 2.7 MATRIZ OPERATIVA DE LAS VARIABLES .................................................... 60 3. MARCO METODOLÓGICO ............................................................................... 61 18 3.1 TIPO DE INVESTIGACIÓN: ............................................................................. 61 3.1.1 Nivel de la Investigación: .............................................................................. 61 3.1.2 Diseño de la Investigación: ........................................................................... 61 3.2 MATERIALES Y MÉTODOS ............................................................................ 61 3.2.1 Fase I preparatoria: ....................................................................................... 61 3.2.2 Fase II descriptiva: ........................................................................................ 62 3.2.3 Fase III interactiva: ........................................................................................ 62 3.2.4 Fase IV analítica: .......................................................................................... 62 3.2.5 Fase de cierre: .............................................................................................. 63 3.3 POBLACIÓN Y MUESTRA .............................................................................. 63 3.3.1 Población. ..................................................................................................... 63 3.3.2 Muestra. ........................................................................................................ 63 3.4 TÉCNICA E INSTRUMENTOS DE RECOLECCIÓN DE DATOS .................... 64 3.5 TÉCNICA DE PROCESAMIENTO Y ANÁLISIS DE DATOS ........................... 64 4. ANÁLISIS E INTERPRETACIÓN DE RESULTADOS ........................................ 65 4.1 RESULTADOS E INTERPRETACIÓN ............................................................. 65 4.1.1 Aislamiento de Amebas de Vida libre de los diferentes tipos de agua de Cúcuta Norte de Santander ................................................................................... 65 4.1.2 Bacilos Acido Alcohol resistentes en el interior de las amebas de vida libre 71 4.2 Discusión ......................................................................................................... 72 5. CONCLUSIONES Y RECOMENDACIONES ..................................................... 76 5.1 CONCLUSIONES ............................................................................................ 76 5.2 RECOMENDACIONES .................................................................................... 76 BIBLIOGRAFIA ...................................................................................................... 78 ANEXOS ................................................................................................................ 85, Ej. 1

Entre os anos 2012 e 2014, a linfadenite granulomatosa (LG) em suínos representou 99% de todas as lesões associadas a zoonoses nos frigoríficos brasileiros. O Mycobacterium avium hominissuis (MAH) é a principal subespécie das micobactérias associada à LG em suínos, com raros casos atribuídos as micobactérias do Complexo Mycobacterium tuberculosis. As lesões macroscópicas, histopatológicas e técnica de Ziehl-Neelsen (ZN) não definem a espécie de micobactéria envolvida na LG. O padrão ouro de diagnóstico é o isolamento de micobactérias, seguido de técnicas moleculares. Este estudo teve como objetivo pesquisar a etiologia da LG no Brasil para atualizar os dados nacionais, e padronizar a técnica de Hibridização in situ Fluorescente (FISH) em linfonodos parafinizados de suínos com LG. No ano de 2017 o Serviço de Inspeção Federal (SIF) coletou em 19 frigoríficos, 637 linfonodos de suínos com lesões granulomatosas, com origem em oito estados brasileiros. As amostras foram submetidas a exame histopatológico, técnica histoquímica de ZN, isolamento de micobactérias, Reação em Cadeia da Polimerase (PCR) e FISH. De acordo com o conhecimento dos autores esta é a primeira tentativa descrita na literatura de padronização da técnica de FISH em tecidos parafinizados de suínos. A lesão histopatológica granulomatosa foi observada em 85,1% dos tecidos linfoides. A taxa de isolamento de micobactérias foi de 32,08% (128/399), destas 76,56% (98/128) origens foram positivas para MAH, 1,56% (2/128) origens positivas para Mycobacterium bovis (M. bovis), 21,87% (28/128) origens positivas e 10 origens negativas para o gênero Mycobacterium spp. Observou-se bacilos-álcool ácido resistentes (BAAR) em 71,7% das amostras de MAH, em 75% das amostras de Mycobacterium spp., e em uma amostra (1/3) M. bovis. Das amostras positivas para BAAR no tecido, 60,47% não isolaram ou foram negativas na técnica da PCR para Mycobacterium spp. A FISH não apresentou resultados positivos em linfonodo parafinizado de suínos. O MAH foi à subespécie mais diagnosticada na LG em suínos do Brasil, com apenas duas origens positivas para M. bovis. Os resultados obtidos neste estudo ficam a disposição para analises de risco, para subsidiar políticas públicas relacionadas à inspeção assim como programas de vigilância de tuberculose suína. Granulomatous lymphadenitis (GL) in pigs represents 99% of all lesions associated with zoonoses in Brazilian slaughterhouses between 2012 and 2014. Mycobacterium avium hominissuis (MAH) is the main subspecies of mycobacteria associated with GL in pigs, and few cases are attributed to the Complex Mycobacterium tuberculosis. The macroscopic, histopathological and Ziehl-Neelsen (ZN) lesions do not define the mycobacterial species involved in GL. The isolation of mycobacteria is the gold standard for diagnosis. Molecular techniques are also used for subspecie determination. The aim of this study was to investigate the etiology of granulomatous lesions for the validation of national data regarding this lesion in pigs and to standardize a fluorescence in situ hybridization (FISH) test in formalin-fixed lymph nodes with granulomatous lesions. In 2017, federal veterinary meat inspectors collected 637 mesenteric lymph nodes with granulomatous lesions from 19 slaughterhouses. Granulomatous histopathological lesions were observed in 85.1% of the lymphoid tissues. The isolation rate of mycobacteria was 32.08%, of these isolates 76.56% were MAH positive, 1.56% for Mycobacterium bovis (M. bovis) and 21,87% for Mycobacterium spp. Acid-alcohol resistant bacillus (BAAR) was observed in 71.7% of the MAH samples, in 75% of the samples of Mycobacterium spp, and in one sample of M. bovis. The standardization of the FISH technique in formalin-fixed porcine lymph nodes was unsuccessful. MAH was the most diagnosed subspecies involved in GL in pigs, with only two positive for M. bovis. The results of this study are available for risk analysis to support inspection-related public policies as well as swine tuberculosis surveillance programs.

O objetivo do presente trabalho foi utilizar métodos bacteriológicos e moleculares para a identificação do Mycobacteriumbovis em lesões observadas em carcaças de bovinos durante a inspeção post mortem de rotina em matadouros-frigoríficos com serviço de inspeção oficial. Foram acompanhados o abate e a inspeção de 825.394 bovinos, sadios, ao exame antemortem pelo serviço de inspeção oficial em 10 matadouros-frigoríficos do estado da Bahia, entre abril de 2009 e abril de 2012. Cento e oitenta bovinos apresentaram lesões sugestivas de tuberculose e outras linfadenites, as quais foram avaliadas quanto à presença de Mycobacterium bovis por exame bacteriológico e pela PCR multiplex. A maioria das lesões estava localizada em linfonodos do trato respiratório e 71% eram provenientes de bovinos machos com até 32 meses de idade. No isolamento bacteriano, 13,9% (25/180) das amostras apresentavam colônias pequenas, de superfície granular e de coloração creme-amareladas, em meio de cultura Stonebrink-Leslie, e o crescimento médio foi de 34 dias. Todos os esfregaços dos isolados evidenciaram BAAR, e, pela PCR multiplex, 56% (14/25) dos isolados foram identificados como M. bovis. A associação entre exame post mortem, bacteriológico e PCR multiplex permitiu a identificação do agente de forma rápida e em regiões com status sanitário de baixa prevalência, demonstrando ser importante para a detecção dos focos de tuberculose bovina e o auxílio nos programas de controle e erradicação da tuberculose. The aim of the present study was to perform bacteriological and molecular methods for identification of Mycobacterium bovis in lesions derived from bovine carcasses detected during routine post-mortem examination in officially inspected slaughterhouses. We checked the slaughter and inspection of 825,394 bovines, health upon ante-mortem examination, by the official service in 10 slaughterhouses of Bahia state from April, 2009 to April 2012. Lesions suggestive of tuberculosis were collected from 180 bovines and further evaluated by bacteriology and multiplex PCR. The majority of lesions were located in the respiratory tract lymph nodes and 71% were from male bovines up to 32 months old. 13.9% of samples presented small, granular and creamy-yellowish colonies after being cultured in Stonebrink-Leslie with an average growth time of 34 days. All smears from the isolated samples were Acid Fast Bacilli (AFB) and among them 56% were identified by mPCR as M. bovis. Thus, the association between post-mortem examination, culture and multiplex PCR allowed the bacillus identification in a reduced time and in regions of low prevalence, pointing out its importance for bovine tuberculosis detection and as a supportive tool for the tuberculosis control and eradication program.

A obtenção de DNA a partir de lâminas é uma alternativa valiosa para resolver o problema do transporte do espécime, principalmente em locais onde a própria geografia é um empecilho para o acesso às amostras, e a reação de PCR tem se destacado como uma promissora técnica molecular para o diagnóstico rápido da tuberculose. Foram selecionadas duzentos e oitenta e sete amostras de conveniência, provenientes do Laboratório da Faculdade de Medicina/Laboratório de Micobactérias/Hospital das Clínicas da Universidade Federal de Minas Gerais. O DNA foi extraído pelo método de Chelex +NP40, e amplifcado pela PCR IS6110 nas seguintes condições: 7,0µL de Buffer (10x), 3,0 µL MgCl2 (50mM), 0,2 µL dNTP (25mM), 25 pmol de cada oligonucleotídeo (IS1- 5 CCT GCG AGC GTA GGC GTC GG 3 e IS2- 5 CTC GTC CAG CGC CGC TTC GG 3) e 0,5 µL de Taq DNA Polimerase (500U) Invitrogen ®, em um volume final de 50 L. A desnaturação incial do DNA, foi a 94°C por 2mim , e um total de 40 ciclos de 94ºC por 30 seg, 68ºC por 2 min, 71ºC for 1mim, e uma extensão final de 72°C por 10min. Os amplicons foram revelados em gel de agarose á 2% corados por brometo de etídio. A sensibilidade e especificidade comparada com a cultura positiva para M. tuberculosis em meio de Lowestein Jensen foram de 37,5 e 100%, respectivamente. O valor preditivo positivo de 100%, valor preditivo negativo de 80,8 % e uma acurácia de 82,8%. A alta especificidade da amplificação do DNA in house, torna este método um procedimento rápido, simples e eficaz para o diagnóstico da tuberculose, podendo ser utilizado em casos de baciloscopias positivas em locais com alta prevalência da Micobactérias Não tuberculosas. Além da biossegurança no transporte, a PCR em lâminas também pode ser aplicada em situações onde a quantidade de células bacterianas é insuficiente para o diagnóstico. Portanto esse método é uma importante ferramenta quando associado a outros critérios diagnósticos, otimizando o tratamento e contribuindo para interrupção da cadeia de transmissão. The obtainment of DNA directly from smear slides is a valuable option to resolve the problem of specimen transportation, as it avoids the risk of bacilli transmission; besides, this characteristic is particularly opportune in places where the geography itself hampers the access to samples and the PCR has emerged as a promising molecular technique for rapid diagnosis of tuberculosis. Two hundred eighty seven convenient samples from the laboratory of the Faculty of Medicine/Laboratory of Mycobacteria/Hospital of Clinics of University Federal of Minas Gerais were selected. The DNA was extracted by the Chelex® + NP40 method and amplified by IS6110-PCR under the following conditions: 7.0 µL of Buffer (10x), 3.0 µL MgCl 2 (50 mM), 0.2 µL dNTP (25 mM), 25pmol of each oligonucleotide (IS1- 5 CCT GCG AGC GTA GGC GTC GG 3 and IS2- 5 CTC GTC CAG CGC CGC TTC GG 3) and 0.5 µL (500 U) of Taq DNA Polymerase Invitrogen ® , in a final volume of 50 L. The initial DNA denaturation was at 94°C for 2 min, following a total of 40 cycles of 94ºC for 30 sec, with every annealing temperature for 2 min, 71ºC for 1 min, and a final extension of 72°C for 10 min. The amplicons were revealed in 2% agarose gel stained by ethidium bromide. The PCR result was then compared with in Lowenstein-Jensen medium. The sensitivity and specificity as compared with those of culture were 37.5 e 100%, respectively. The positive predictive value was of 100%, the negative predictive value was of 80.8%, and the accuracy was of 82.8%. The high specificity of in house DNA amplification becomes this method, a quick and simple procedure for tuberculosis diagnosis; it could also assure the utilization of this method in places in which the bacilloscopy is positive, with a higher prevalence of Nontuberculous Mycobacteria. Besides to offer biosafety in the transportation, the in house DNA amplification could also be utilized in situations where the amount of bacterial cells is insufficient. Therefore, this method is an important tool when associated to clinical criteria, speeds up the treatment andcontributes to interrupt the disease transmission chain.

O diagnóstico histoquímico de micobacterioses, através da visualização de bacilos álcool-ácido resistentes (BAAR), em esfregaços corados mediante a técnica de Ziehl-Neelsen, a partir de amostras de tecidos, é considerada como uma boa técnica de diagnóstico presuntivo. Neste estudo pretendeu-se avaliar a frequência de visualização de BAAR em rim e gânglios linfáticos mesentéricos com lesões granulomatosas em veados (Cervus elaphus) e javalis (Sus scrofa). Os esfregaços de cada órgão foram efectuados sobre uma lâmina de vidro e as preparações foram coradas de acordo com o método de Ziehl-Neelsen. Dos 91 esfregaços de gânglios mesentéricos analisados, 46 (50,5%) mostraram BAAR compatíveis, sendo classificados como positivos. Vinte e dois esfregaços de rim dos 25 analisados (88%) apresentaram BAAR. Dos 25 animais, em que foram observados simultaneamente esfregaços de gânglios mesentéricos e rim, 15 (60%) apresentaram resultados coincidentes. Em javalis, a frequência de esfregaços, classificados como positivos, foi de 76,2% (32/42) e em veados de 48,6% (36/74). Neste estudo, o rim revelou-se o órgão com maior frequência de visualização de BAAR, e o javali como a espécie animal que apresentou maior percentagem de resultados positivos.

Dissertation thesis Leading Figures and Local Culture in Czech Regions presents a comparative analysis of two original Czech regions, Chodsko and the Bohemian Forest, and their spiritual leaders, Jindřich Šimon Baar and Karel Klostermann. Main part of the thesis consists of analysis of life, work and contribution of these local icons in context of the geographical, historical and cultural aspects of regions concerned. Emphasis is placed on the specifics of local folk literature, folklore and material culture. The theoretical part is complemented by deep research in both regions, in the form of a questionnaire completed by hundred students, which answers questions regarding their involvement in folk life and experience of their regional identity. Keywords: local culture, Chodsko, Bohemian Forest, Baar, Klostermann

Baden-Württemberg besitzt als eines der letzten moorreichen Bundesländer der Bundesrepublik kein eigenständiges, naturschutzfachliches Programm zum Schutz seiner Moore. Aus diesem Anlass wurde eine Methodik zur Ableitung von Restitutionspotenzialen entwickelt, die als Steuerungselement bei der zukünftigen Umsetzung von Maßnahmen dienen soll. Dabei standen zentral folgende Fragestellungen im Mittelpunkt der Untersuchung: Welche Faktoren bedingen das Restitutionspotenzial eines Moores, beziehungsweise einer Teilfläche eines Moores? In welcher Form kann die Datenmatrix zur Ableitung des Restitutionspotenzials zusammengeführt werden, um zu einer transparenten Bewertung zu gelangen? Welche moorökologischen Daten sind landesweit in welcher Qualität vorhanden? Sind diese ausreichend, um zu einer Prioritätensetzung für Moorrestitutionen zu gelangen? Die Methodik wurde exemplarisch für das Naturschutzgebiet ?Schwenninger Moos? abgeleitet. Die Ergebnisse wurden anschließend auf den gesamten, umgebenden Naturraum, die Baar und Baaralb übertragen. Durch diese Verifizierung der Methodik konnten erhebliche Defizite im landesweiten Datensatz aufgezeigt werden. Als Grundlage zur Bewertung des Restitutionspotenzials wurden im Schwenninger Moos vor allem Daten zum Wasserhaushalt, Trophie und Arten und Biotopen erhoben. Der Wasserhaushalt des Schwenninger Mooses wurde dabei durch 34 Pegel dokumentiert. Besonders gut geeignet zur Charakterisierung der Standorte erwiesen sich dabei Halbjahresmediane und die kombinierte Betrachtung von Amplitude, Mittelwert und Tiefstwert. Die Trophie der Standorte wurde durch Messungen der Hauptnährstoffe, pH- und Leitfähigkeitswert sowie über Angaben zum C/N-Verhältnis verglichen. Die Vegetation des Untersuchungsgebietes wurde vergleichend nach der Biotoptypenkartierung des Landes Baden-Württemberg, der Methodik zur Erfassung von Lebensraumtypen der FFH-Richtlinie sowie nach indikatorischen Artgruppen ausgewertet. Vor allem die Kartierung indikatorischer Artgruppen eignet sich zur Beurteilung des aktuellen Zustandes eines Moorkomplexes. Die vorliegenden Daten zur Torflagerstätte aus dem moorgeologischen Kataster wiesen ein hohes Alter auf. Deshalb wurden diese Daten durch Vergleichsbohrungen und kontinuierliche Messungen mittels Georadar überprüft. Dabei ergaben sich zum Teil erhebliche Differenzen zwischen den Angaben des moorgeologischen Katasters und den eigenen Untersuchungen. Die Ableitung des Restitutionspotenzials erfolgte durch die Kombination von drei Einzelbewertungen: Wiedervernässbarkeit, biotisches Potenzial und Umsetzungspotenzial. Diese drei Faktoren werden verbal-argumantativ abgeleitet und in einer fünf-stufigen Klassifikation zusammengeführt. Wesentliche Größe ist dabei die Wiedervernässbarkeit einer Fläche, die von Entwässerungseinrichtungen, Relief, Zustand der Torfe und dem Wasserdargebot abhängt. Das biotische Potenzial besteht aus dem Vorhandensein von Torfbildnern und den Auswirkungen einer Wiedervernässung. Das Umsetzungspotenzial ist im Wesentlichen von den herrschenden Nutzungen, Eigentumsverhältnissen und gesellschaftlichen Rahmenbedingungen abhängig. Die Methodik wurde auf 34 Moorkomplexe der Baar und Baaralb übertragen. Die Identifizierung dieser Standorte war erst durch eine Zusammenführung unterschiedlicher Datensätze möglich. Es zeigte sich, dass das moorgeologische Kataster auf der Baar erhebliche Defizite bei der Ausweisung kleiner und flachgründiger Standorte aufwies. Nur 3 der 34 Standorte wiesen ein hohes Restitutionspotenzial auf. 23,5 % wurden mit einem mittleren Restitutionspotenzial eingestuft. Bei diesen Mooren handelt es sich im Wesentlichen um Naturschutzgebiete und flächenhafte Naturdenkmale. Ein geringes Restitutionspotenzial ist bei 38,2 % der Moorkomplexe vorhanden. Insgesamt 29,4 % der Moore wiesen kein Restitutionspotenzial mehr auf. Für die Umsetzung eines landesweiten Moorschutzprogramms sind im Rahmen der Untersuchung die wesentlichen Defizite aufgezeigt und Handlungsempfehlungen formuliert worden. Es muss primär ein aktualisiertes und vollständiges Moorkataster zur Verfügung stehen. In den naturschutzfachlich hochwertigen Moorkomplexen sollten zudem flächige Kartierungen der Vegetation und wertgebender Arten vorliegen. Es ist in diesem Zusammenhang ein Indikationsmodell von Vegetationseinheiten süddeutscher Moore anzustreben. Die Bearbeitung von Prioritätslisten sind von regionalen Expertengruppen für die jeweiligen Moorregionen oder Landkreise vorzunehmen. As one of the last peatland-rich states of Germany, Baden-Württemberg possesses no self-standing, conservation program to protect its peatlands. Therefore, after consideration of the state administration, a strategy should be created in the next few years for a Baden-Württemberg peatland protection concept. With this in mind, a methodology for the derivation of peatland restitution potential was developed that can direct the future implementation of such a strategy. The exemplary methodology was developed for the nature conservation area ?Schwenninger Moos?, a medium sized former raised bog strongly disturbed by peat digging and agricultural amelioration. The results were applied to the entire surrounding bio-geographical region, the Baar and the Baaralb. Through this verification of the methodology, significant shortfalls in the state-wide dataset could become evident. As the basis of the evaluation of the restitution potential, data concerning the water level, trophy, and species and biotypes in Schwenninger Moos were collected. The water level of Schwenninger Moos was documented through 34 water gauges, the data from which was analysed through various methods. The half-year median and the combined examination of amplitude, average, and minimum values proved especially suitable for the characterisation of the sites. The trophic classifications of the sites were compared through measuring the principal nutrient levels, pH and conductivity values, as well as the C/N ratio. The vegetation of the investigation area was mapped and the results were subsequently compared to the mapping of biotypes of Baden-Württemberg, the mapping performed in the frame of Natura 2000, as well as that of indicator species groups. The mapping of indicator-species groups proved particularly suitable for the appraisal of the current status of a moor complex. The available data on the mires from the peatland register of Baden-Württemberg were somewhat old (40 years). Therefore these data were verified through comparison-boring and continued measurement by means of georadar. Significant differences arose in a number of comparisons between the peatland register and the author?s investigations with respect to the positional accuracy and the stratum sequence. The derivation of the restitution potential was carried out through the combination of three separate assessments: rewetting-possibility, biotic potential, and conversion potential. These three factors were derived through verbal-argument and brought together in a simple, five-step classification. The rewetting-possibility of an area is the main factor determining the restitution, and it depends considerably on drainage systems, topography, condition of the peat, and the water level. The biotic potential consists of the presence of peat-producing plants and the effects of rewetting on populations of endangered species. The conversion potential is essentially dependent on the dominant uses, ownership and social framework. The methodology was applied to altogether 34 peatland complexes of the Baar and Baaralb. The identification of these locations was only possible by a laborious combination of the peatland register, pedological cartography and conservation-oriented publications. It was found that the peatland register exhibited substantial deficits regarding the classification of small and shallow-layered locations in the Baar. Data concerning vegetation and the occurrence of animals and plants were available for the derivation of the restitution potential. However, some of these proved too old and/or too inaccurate or not spatially verified. Little to no data were present as to the water balance and drainage systems. Only three of the 34 locations exhibited a high restitution potential. 23,5% were classified as having moderate restitution potential; most of these sites are already under protection as nature conservation sites. A slight restitution potential was present in a total of 38.2% of the mire complexes, which consist mostly of small-scale spring mires and intensively agriculturally and silviculturally used locations. Likewise, 29.4% of the moorlands exhibited no more restitution potential. For the implementation of a state-wide mire protection program in the context of the investigation, the substantial deficits were pointed out and recommendations for action were formulated. Primarily, an updated and complete peatland register must be available. Furthermore, vegetation and endangered species maps of the entire peatland complexes protected as high conservation value areas should be available. It is in this regard that a system of indicator vegetation units of south German mires is to be aimed for, as is employed in northeast Germany, for example. Lists of priority sites should be made by regional teams of experts for the respective moor regions or administrative districts.