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We report here one new, hospitalized case of Anaplasma phagocytophilum in Belgium. The clinical presentation of anaplasmosis, its treatment and the molecular and serological relevant laboratory methods are briefly developed.

Propionic acid producing strains of Corynebacterium minutissimum were isolated from three patients with opportunistic infections. One neutropenic patient was undergoing chemotherapy for prolymphocytic leukemia; the other two patients were undergoing hemodialysis and peritoneal dialysis respectively. An unusual feature of these three strains was their resistance to several antibiotics, which is seldom seen in diphtheroids other than Corynebacterium jeikeium and CDC group D2.

Four identification tests, proposed in addition to conventional methods, were evaluated with 320 fermentative nonlipophilic Corynebacterium strains: growth at 20°C, glucose fermentation at 42°C, alkalinization of sodium formate, and acid production from ethylene glycol. These tests were highly discriminant. Corynebacterium amycolatum displayed a unique profile, allowing it to be distinguished from similar species, such as C. xerosis , C. striatum , and C. minutissimum.

Dans cette etude, un total de 154 souches de Streptococcus pneumoniae provenant de 8 centres differents dans la Province d'Hainaut a ete evalue en ce qui concerne la sensibilite pour la penicilline, l'amoxicilline, la cefuroxime, la ciprofloxacine, la moxifloxacine, l'erythromycine et la tetracycline. La sensibilite a ete determinee par une technique de microdilution d'apres les criteres de NCCLS. Le taux global de sensibilite diminuee a la penicilline etait de 32.5% (23.4% de souches intermediaires et 9.1% de souches resistantes). Le taux global de sensibilite diminuee pour les autres antibiotiques etait : amoxicilline 1.9% [0 % Resistance (R)], cefuroxime 23.4% (R 22.1%), ciprofloxacine 9.1 % (R 1.3%), erythromycine 39.6% (R 38.3%) et tetracycline 31.8% (R 30.5%). Pour la moxifloxacine, une sensibilite diminuee n'etait pas detectee. Les CMI de l'amoxicilline, cefuroxime, erythromycine et tetracycline augmentaient avec celles de la penicilline pour les souches avec une sensibilite diminuee pour la penicilline. La plupart des souches avec une sensibilite diminuee pour la penicilline restait sensible a l'amoxicilline (94%) tandis que la moxifloxacine et la ciprofloxacine gardaient leur activite respectivement sur 100% et 92% de ces souches. Les souches avec une sensibilite diminuee pour la penicilline etaient 36,7%, 30.0% et 3,3% co-insensibles pour respectivement l'erythromycine, la tetracycline et la ciprofloxacine.

Brevibacterium otitidis is a coryneform rod and, as far as is known, is isolated only from infected ears. We report the first known case of peritonitis caused by B. otitidis in a patient undergoing continuous ambulatory peritoneal dialysis.

We describe 2 patients presenting lumbar spondylitis due to Streptococcus milleri. In both cases origin was related to preexistent intestinal pathology. Surgical drainage of a collection of pus was necessary in one case. Longterm antibiotic therapy led to full recovery. Despite confused nomenclature Streptococcus milleri must be considered a serious pathogen mainly associated with purulent infection with osteoarticular affinity.

Resume 27 souches de corynebacteries multiresistantes provenant de malades hospitalises ont ete etudiees. 20 appartiennent au groupe des « diphteroides JK , les 7 autres correspondent a un groupe non decrit, possedant une urease tres active. Toutes sont resistantes a la plupart des antibiotiques mais sensibles a la vancomycine. Trois de ces souches proviennent d'hemocultures positives repetees chez des malades hematologiques en etat de septicemie et presentant une neutropenie. Une souche provient d'une infection peritoneale recidivante chez un dialyse. Les autres souches doivent etre considerees comme simples agents de colonisation, parfois avec bacteriemie transitoire.

SummaryArcanobacterium haemolyticum (Corynebacterium haemolyticum) and Streptococcus milleri were isolated from the blood of a 15-year old boy presenting with unilateral tonsillitis, pulmonary abscesses and septicaemia. This report summarizes the clinical presentation and the bacteriological findings of the case.

Resume Les auteurs rapportent l'isolement de quatre souches d' Arcanobacterium haemolyticum (Corynebacterium haemolyticum) a partir de deux cultures de frottis de gorge, d'une hemoculture et d'une culture de pus. L'identification biochimique de ce bacille coryneforme beta-hemolytique et ne possedant pas de catalase a ete confirmee par l'emploi du test d'inhibition de l'hemolysine staphylococcique et de la recherche d'une synergie hemolytique avec Streptococcus agalactiae . Ces quatre observations permettent de completer des donnees epidemiologiques concernant la situation de ce germe en Europe.

SummaryWe report a case of group JK Diphteroids and Candida albicans sepsis in a leukaemic patient under chemotherapy.This case stresses the interest of identification of Diphteroids isolated repeatedly from blood cultures.

A total of 38 strains of Corynebacterium Group D2 isolated from clinical specimens in Belgian laboratories were characterized by pronounced urease activity, inability to acidify sugars and to reduce nitrates and multiresistance to antibiotics. Two strains were involved in clinical diseases. The organisms were found to have a more opportunistic than pathogenic behaviour.