1. Prognostic value of a pre-freeze hypo-osmotic swelling test on the post-thaw quality of dog semen
- Author
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M. Grau, S Karger, O. Burfeind, B Geiser, Wolfgang Heuwieser, and Sebastian Arlt
- Subjects
Male ,endocrine system ,Motility ,Semen ,Semen analysis ,Biology ,Cell morphology ,Cryopreservation ,Andrology ,03 medical and health sciences ,Semen quality ,Dogs ,0302 clinical medicine ,Endocrinology ,Food Animals ,Osmotic Pressure ,Predictive Value of Tests ,Freezing ,medicine ,Animals ,Osmotic pressure ,Cell Size ,030219 obstetrics & reproductive medicine ,medicine.diagnostic_test ,urogenital system ,0402 animal and dairy science ,Water ,04 agricultural and veterinary sciences ,General Medicine ,Prognosis ,Spermatozoa ,040201 dairy & animal science ,Sperm ,Semen Analysis ,Animal Science and Zoology ,Semen Preservation - Abstract
Throughout cryopreservation, sperm are exposed to major osmotic challenges. Only intact membranes of sperm cells are able to regulate these volumetric changes, which can be determined by the hypo-osmotic swelling test (HOS test). Correlations between the HOS test and conventional semen variables are inconsistent. Therefore, the objectives of this study were (1) to examine relationships between HOS test results and standard semen variables before freezing and after thawing and (2) to evaluate the prognostic value of the HOS assessments on post-thaw quality of dog semen. Semen of 35 dogs was collected and analyzed before freezing and after thawing following a 7-day freeze-thaw interval. Conventional semen variables such as sperm cell motility, membrane integrity morphology were evaluated and the HOS test was conducted with results from this test being recorded. In fresh semen the HOS test was positively correlated with progressive motility of sperm cells: r=0.52, sperm cell membrane integrity: r=0.50 and normal sperm cell morphology: r=0.46 (P
- Published
- 2016