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BACKGROUND: The incidence of tinnitus has been increasing together with its patient impact and societal costs. Much research has been conducted in the field of tinnitus, especially on treatment modalities because there still is no cure. This study aims to analyze the evolutions and developments in the scientific output relating to tinnitus. METHODS: We analyzed the Science Citation Index Expanded featured articles in the Web of Science Core Collection relating to tinnitus from 1980 to 2020. The publications were analyzed by characteristics such as the countries and institutions, journals, the most cited articles and references, and the most frequently used words in the abstracts and keywords. RESULTS: In total, 8282 articles relating to tinnitus were identified in the Web of Science. The number of publications has been significantly increasing after the 1990s. Of the 8282 articles, a major part originated from the American and European institutions. Most articles originated from high-impact journals, which consequently also covered the most cited papers. A major interest was seen in areas about treatment and pathogenic mechanisms. CONCLUSION: This bibliometric analysis here indicated an increasing trend in tinnitus research from 1980 to 2020, particularly with the increase in tinnitus burden and the societal costs by it. Specific interest has been seen in the specific tinnitus pathophysiological mechanisms and treatment. Individual researchers and institutions will gain a new perspective on their future studies based on the bibliometric data in our paper.

Additional file 1: Figure S1. PRRX1 expression in HCC. Figure S2. Analysis of PRRX1 expression with respect to clinicopathological variables, and in experimental models. Figure S3. Cellular components in which are involved genes positively and negatively correlated with PRRX1 in TCGA liver cancer data. Figure S4. KEGG pathway annotation of PRRX1 correlated genes in various cancers where its alteration frequency is high. Figure S5. Annotation of PRRX1 inversely correlated genes. Figure S6. Correlation and survival analyses of PRRX1 and its co-expressed genes. Figure S7. Survival analysis and expression of ZEB1/2 also with respect to TP53 and CTNNB1 mutation. Figure 8S. PRRX1 and metabolic targets. Table S1. Human HCC microarrays. Table S3. KEGG pathway annotation of genes co-expressed (positively) with PRRX1. Table S4. KEGG pathway annotation of genes inversely correlated with PRRX1. Table S5. GO Biological processes for PRRX1 positively co-expressed genes. Table S6. GO Cellular components for PRRX1 positively co-expressed genes. Table S7. GO Biological processes for PRRX1 inversely correlated genes. Table S8. GO Cellular components for PRRX1 inversely correlated genes. Table S9. Metabolic targets in the PRRX1 co-expressed gene list. Table S11. 148 genes as potential candidates that likely cooperate or are coregulated with PRRX1. Table S12. ZEB1 expression in combination with PRRX1 with respect to the clinicopathological variables (n= number of patients). Table S13. ZEB2 expression in combination with PRRX1 with respect to the clinicopathological variables (n= number of patients).

Global change scenarios in the Mediterranean basin predict a precipitation reduction within the coming hundred years. Therefore, increased drought will affect forests both in terms of adaptive ecology and ecosystemic services. However, how vegetation might adapt to drought is poorly understood. In this report, four years of climate change was simulated by excluding 35% of precipitation above a downy oak forest. RNASeq data allowed us to assemble a genome-guided transcriptome. This led to the identification of differentially expressed features, which was supported by the characterization of target metabolites using a metabolomics approach. We provided 2.5 Tb of RNASeq data and the assembly of the first genome guided transcriptome of Quercus pubescens. Up to 5724 differentially expressed transcripts were obtained, 42 involved in plant response to drought. Transcript set enrichment analysis showed that drought induces an increase in oxidative pressure that is mitigated by the upregulation of ubiquitin-like protein protease, ferrochelatase, oxaloacetate decarboxylase and oxo-acid-lyase activities. Furthermore, the downregulation of auxin biosynthesis and transport, carbohydrate storage metabolism were observed as well as the concomitant accumulation of metabolites, such as oxalic acid, malate and isocitrate. Our data suggest that early metabolic changes in the resistance of Q. pubescens to drought involve a tricarboxylic acid (TCA) cycle shunt through the glyoxylate pathway, galactose metabolism by reducing carbohydrate storage and increased proteolytic activity.