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En el presente trabajo se describen cuatro especies de dinoflagelados de la familia Peridinaceae: Peridiniopsis elpatiewskyi (Ostenfeld) Bourrelly y P. cunningtonii Lemmermann, para la represa Poechos, zona costera en el norte de Perú; Peridinium gatunense Nygaard y Parvodinium umbonatum (Stein) Carty, para la represa Tahuín, suroeste de Ecuador. Se estudió la morfología celular, así como el número y la posición de sus placas intercalares, cingulares y sulcales. Se describen por primera vez para el Perú la presencia de P. elpatiewskyi y P. cunningtonii.

En el presente trabajo se describen cuatro especies de dinoflagelados de la familia Peridinaceae: Peridiniopsis elpatiewskyi (Ostenfeld) Bourrelly y P. cunningtonii Lemmermann, para la represa Poechos, zona costera en el norte de Peru; Peridinium gatunense Nygaard y Parvodinium umbonatum (Stein) Carty, para la represa Tahuin, suroeste de Ecuador. Se estudio la morfologia celular, asi como el numero y la posicion de sus placas intercalares, cingulares y sulcales. Se describen por primera vez para el Peru la presencia de P. elpatiewskyi y P. cunningtonii.

En el presente trabajo se registra por primera vez el dinoflagelado Tripos dens (Ostenfeld & Schmidt) F. Gómez 2013, en la zona norte del Perú (Máncora-Piura-Perú) a partir del 2013. La identificación de T. dens se realizó mediante un examen morfo-taxonómico detallando sus placas con microscopía de luz; también se comparó con T. balechii, especie con la que se confundió en diversas publicaciones.

The "Wells syndrome" is a rare inflammatory dermatosis of unknown pathogenesis, clinical polymorph, both the location and the extent of the lesions. This is a skin disease of inflammatory origin, which usually appears in children and adolescents. Has a polymorphic clinical picture, both the location and the extent of the lesions, and for the correct interpretation of diagnostic histopathological lesions required. This entity should be considered in the differential diagnosis of any atypical presentation of cellulite with peripheral eosinophilia, that does not respond to antibiotics.

Enteroviruses induce the formation of membranous structures (replication organelles [ROs]) with a unique protein and lipid composition specialized for genome replication. Electron microscopy has revealed the morphology of enterovirus ROs, and immunofluorescence studies have been conducted to investigate their origin and formation. Yet, immunofluorescence analysis of fixed cells results in a rather static view of RO formation, and the results may be compromised by immunolabeling artifacts. While live-cell imaging of ROs would be preferred, enteroviruses encoding a membrane-anchored viral protein fused to a large fluorescent reporter have thus far not been described. Here, we tackled this constraint by introducing a small tag from a split-GFP system into an RO-resident enterovirus protein. This new tool bridges a methodological gap by circumventing the need for immunolabeling fixed cells and allows the study of the dynamics and formation of enterovirus ROs in living cells., Like all other positive-strand RNA viruses, enteroviruses generate new organelles (replication organelles [ROs]) with a unique protein and lipid composition on which they multiply their viral genome. Suitable tools for live-cell imaging of enterovirus ROs are currently unavailable, as recombinant enteroviruses that carry genes that encode RO-anchored viral proteins tagged with fluorescent reporters have not been reported thus far. To overcome this limitation, we used a split green fluorescent protein (split-GFP) system, comprising a large fragment [strands 1 to 10; GFP(S1-10)] and a small fragment [strand 11; GFP(S11)] of only 16 residues. The GFP(S11) (GFP with S11 fragment) fragment was inserted into the 3A protein of the enterovirus coxsackievirus B3 (CVB3), while the large fragment was supplied by transient or stable expression in cells. The introduction of GFP(S11) did not affect the known functions of 3A when expressed in isolation. Using correlative light electron microscopy (CLEM), we showed that GFP fluorescence was detected at ROs, whose morphologies are essentially identical to those previously observed for wild-type CVB3, indicating that GFP(S11)-tagged 3A proteins assemble with GFP(S1-10) to form GFP for illumination of bona fide ROs. It is well established that enterovirus infection leads to Golgi disintegration. Through live-cell imaging of infected cells expressing an mCherry-tagged Golgi marker, we monitored RO development and revealed the dynamics of Golgi disassembly in real time. Having demonstrated the suitability of this virus for imaging ROs, we constructed a CVB3 encoding GFP(S1-10) and GFP(S11)-tagged 3A to bypass the need to express GFP(S1-10) prior to infection. These tools will have multiple applications in future studies on the origin, location, and function of enterovirus ROs. IMPORTANCE Enteroviruses induce the formation of membranous structures (replication organelles [ROs]) with a unique protein and lipid composition specialized for genome replication. Electron microscopy has revealed the morphology of enterovirus ROs, and immunofluorescence studies have been conducted to investigate their origin and formation. Yet, immunofluorescence analysis of fixed cells results in a rather static view of RO formation, and the results may be compromised by immunolabeling artifacts. While live-cell imaging of ROs would be preferred, enteroviruses encoding a membrane-anchored viral protein fused to a large fluorescent reporter have thus far not been described. Here, we tackled this constraint by introducing a small tag from a split-GFP system into an RO-resident enterovirus protein. This new tool bridges a methodological gap by circumventing the need for immunolabeling fixed cells and allows the study of the dynamics and formation of enterovirus ROs in living cells.

The hydrography of the Indian-Atlantic Ocean gateway has been connected to high-latitude climate dynamics by oceanic and atmospheric teleconnections on orbital and suborbital timescales. A wealth of sedimentary records aiming at reconstructing the late Pleistocene paleoceanography around the southern African continent has been devoted to understanding these linkages. Most of the records are, however, clustered close to the southern South African tip, with comparatively less attention devoted to areas under the direct influence of frontal zones of the Southern Ocean/South Atlantic. Here we present data of the composition and concentration of the diatom assemblage together with bulk biogenic content and the alkenone-based sea surface temperature (SST) variations for the past 350 kyr in the marine sediment core MD02-2588 (approximately 41°S, 26°E) recovered from the southern Agulhas Plateau. Variations in biosiliceous productivity show a varying degree of coupling with Southern Hemisphere paleoclimate records following a glacial-interglacial cyclicity. Ecologically well-constrained groups of diatoms record the glacial-interglacial changes in water masses dynamics, nutrient availability, and stratification of the upper ocean. The good match between the glacial maxima of total diatoms concentration, Chaetoceros spores abundance, and opal content with the maximum seasonal cover of Antarctic ice and the atmospheric dust records points to a dominant Southern Hemisphere forcing of diatom production. Suborbital variability of SST suggests rapid latitudinal migrations of the Subtropical Front and associated water masses over the southern Agulhas Plateau, following millennial contractions and expansions of the subtropical gyres. Warmings of the upper ocean over site MD02-2588 during terminations IV to I occurred earlier than that in the Antarctic Vostok, which is indicative of a Northern Hemisphere lead. Our multiparameter reconstruction highlights how high-latitude atmospheric and hydrographic processes modulated orbital highs and lows in primary production and SST as triggered by northward transport of Si, eolian dust input, and latitudinal migrations of frontal zones.

High-quality services must keep working reliably and efficiently, and service compositions are no exception. As they integrate several internal and external services over the network, they need to be carefully designed to meet their performance requirements. Current approaches assist developers in estimating whether the selected services can fulfill those requirements. However, they do not help developers define requirements for services lacking performance constraints and historical data. Manually estimating these constraints is a time-consuming process which might underestimate or overestimate the required performance, incurring in additional costs. This work presents the first version of two algorithms which infer the missing performance constraints from a service composition model. The algorithms are designed to spread equally the load across the composition according to the probability and frequency each service is invoked, and to check the consistency of the performance constraints of each service with those of the composition.

En este trabajo se describe el resultado de la determinación de antitrombina III (ATIII) en veintitrés casos de anemia hemolítica asociada a estados de hipercoagulabilidad sanguínea. El 74% de los pacientes presentaron tasas bajas de ATIII y por lo tanto presentaban riesgos de desarrollar trombosis o coagulaciones intravasculares diseminadas (CID)..