42 results on '"B, Charfeddine"'
Search Results
2. Étude comparative de la protéine C-réactive et de la procalcitonine dans le diagnostic de sévérité des pyélonéphrites aiguës de l’enfant
- Author
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K. Limam, J. Bouguila, K. Chatti, L. Boughammoura, B. Charfeddine, M. Ben Rejeb, H. Essabbeh, I. Khalef, and A. S. Essoussi
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Gynecology ,medicine.medical_specialty ,business.industry ,medicine ,General Medicine ,business - Abstract
Resume Objectif L’objectif de ce travail est de comparer deux parametres biologiques, la proteine C-reactive (CRP) et la procalcitonine (PCT) dans la detection des lesions renales aigues confirmees par la scintigraphie au DMSA, dans les pyelonephrites aigues (PNA) chez l’enfant. Patients et methodes Etude prospective menee sur une annee, incluant les enfants presentant un premier episode de PNA. Tous les enfants ont eu un dosage de la PCT, la CRP et l’hemogramme. Resultats Parmi les 75 patients inclus, 33 avaient des lesions renales aigues (groupe A) et 42 enfants avaient une scintigraphie au DMSA normale (groupe B). Dans le groupe A, la valeur moyenne de la PCT etait plus elevee que dans le groupe B avec une difference significative (8,81 ng/mL versus 1,7 ng/mL, p = 0,01). Le calcul de l’air sous la courbe ROC de la CRP et de la PCT a trouve que la PCT a un seuil de 0,76 ng/mL etait plus performante dans la detection des lesions renales aigues par rapport a la CRP a un seuil de 70 mg/L avec une meilleure sensibilite, valeur predictive negative et indice de Youden (82 % versus 70 % ; 84 % versus 70 % et 0,58 versus 0,25). Conclusions Notre etude a confirme la bonne sensibilite et la bonne specificite du dosage de la PCT dans le diagnostic des lesions aigues du parenchyme renal des infections urinaires de l’enfant febrile.
- Published
- 2013
3. Paramètres du stress oxydant dans le diabète de type 2
- Author
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A. Miled, L. Chaieb, A. Omezzine, Sandrine Laradi, H. Ammar, Asma Kassab, S. Ferchichi, and B Charfeddine
- Subjects
chemistry.chemical_classification ,Chemistry ,Glutathione peroxidase ,Biochemistry (medical) ,Clinical Biochemistry ,Non insulin dependent diabetes mellitus ,lipids (amino acids, peptides, and proteins) ,Molecular biology - Abstract
Resume Introduction. – Des etudes recentes ont montre l’intervention des radicaux libres dans la genese des complications chroniques du diabete sucre. Il est admis que les LDL oxydees sont impliquees dans l’induction de l’atherosclerose. But. – Notre travail vise a mettre en evidence la variation des parametres metaboliques, le statut anti-oxydant et oxydant chez des diabetiques non insulinodependants (DNID). Methodes. – Cette etude a porte sur 39 DNID compares a 41 sujets temoins. La glycemie, l’hemoglobine glyquee (HbA1c), l’uree, la creatinine, l’acide urique, les triglycerides (TG), le cholesterol total (Chol-T), le HDL-cholesterol (HDL-chol), le LDL-cholesterol (LDL-chol), l’apolipoproteine A1 (Apo A1), l’apolipoproteine B (Apo B), le statut anti-oxydant total (Sat), les substances reagissant avec l’acide thiobarbiturique liees aux LDL (LDL-TBARS), l’ α-tocopherol, le cuivre et le zinc plasmatiques ont ete mesures ainsi que l’activite enzymatique de la superoxyde dismutase (Sod) et de la gluthation peroxydase (GPX). Resultats. – Dans la population diabetique etudiee par rapport aux temoins, l’activite de la GPX erythrocytaire et la concentration en uree plasmatique etaient inchangees, le statut anti-oxydant total (Sat), l’activite de Sod erythrocytaire, l’α-tocopherol, le zinc etaient significativement diminues. La concentration des LDL-TBARS etait augmentee. Conclusion. – La diminution des parametres Sat, Sod et l’augmentation des LDL-TBARS semblent constituer un signe d’alarme des complications graves qui apparaitront a moyen et long terme chez les DNID.
- Published
- 2003
4. [Comparative study of C-reactive protein and procalcitonin in the severity diagnosis of pyelonephritis in children]
- Author
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J, Bouguila, I, Khalef, B, Charfeddine, M, Ben Rejeb, K, Chatti, K, Limam, H, Essabbeh, A S, Essoussi, and L, Boughammoura
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Calcitonin ,Male ,Adolescent ,Pyelonephritis ,Calcitonin Gene-Related Peptide ,Infant, Newborn ,Infant ,Sensitivity and Specificity ,Severity of Illness Index ,C-Reactive Protein ,Predictive Value of Tests ,Child, Preschool ,Humans ,Female ,Protein Precursors ,Child ,Radionuclide Imaging ,Succimer - Abstract
The aim of this study is to compare two biologic parameters; C-reactive protein (CRP) and procalcitonin (PCT) in the detection of acute renal lesions assessed by DMSA scintigraphy in the urinary tract infection in child.In a prospective study, serum PCT, CRP and leukocyte counts were measured for children admitted, between January and December 2010, with a first episode of febrile urinary tract infection.Seventy-five children were enrolled in the study. Thirty-three patients had renal lesions (group A) and 42 had a normal DMSA scintigraphy (group B). The mean PCT level was significantly higher in group A than in group B (8.81 ng/mL versus 1.7 ng/mL, P=0.01). In this study, using receiver operating characteristic (ROC) curve, we identified that the optimal cut-off value with ideal sensitivity and specificity for PCT in detection of renal lesions was 0.76 ng/mL and for CRP, it was 70 mg/L. The sensitivity, the negative predictive value and the indice of Youden of the cut-off value of PCT were significantly higher than CRP (82% versus 70%; 84% versus 70% and 0.58 versus 0.25).This study confirmed that the serum PCT level was more sensitive and specific than the CRP in the detection of renal lesions in the first urinary tract infection in child.
- Published
- 2012
5. Résistance du facteur V à la protéine C activée au cours des avortements à répétition
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A Mahdaoui, K Monastiri, Sandrine Laradi, T Mahjoub, B Charfeddine, R Maarouf, and F Ben Haj Slama
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Gynecology ,medicine.medical_specialty ,biology ,business.industry ,Biochemistry (medical) ,Clinical Biochemistry ,Factor V ,Abortion ,medicine.disease ,Miscarriage ,Pathogenesis ,Coagulation ,Immunology ,biology.protein ,Factor V Leiden ,Medicine ,Activated protein C resistance ,business ,Protein C ,medicine.drug - Abstract
Activated protein C resistance of factor V in case of recurrent pregnancy loss. Both miscarriage and still birth are a common event with many possible causes. Actually, thrombophilic disorders are more and more well established. The association between antiphospholipid syndrom and recurrent spontaneous abortion has been demonstrated but the thrombophilic origin due to an inherited defect in anticoagulation known as activated protein C resistance to factor V (APCR), recently described, is still on discussion. Objective: to determine whether women with factor V Leiden mutation, a common inherited defect of coagulation are at increased risk for recurrent pregnancy loss. Patients: 54 tunisian women presenting recurrent spontaneous abortion have been studied. Methods: the presence of antibodies directed against phospholipids has been investigated with a coagulation method and an immunologic method (ELISA). The study of activated protein C resistance to factor V has been determined with the Accelerimat® (Biomerieux) and the genetic study by polymerase chain reaction (PCR). Results : the screening of antiphospholipids has been negative for the 54 women. These 6 ladies whether 11.11 % of the patients have presented an activated protein C resistance to factor V ; everyone of them was heterozygous for the factor V Leiden mutation. Conclusion : our study supports the hypothesis which associates the repetition abortion and the factor V Leiden. The investigation of APCR can be indicated in the research of repeated abortion etiologies.
- Published
- 2001
6. [Homocysteine, vitamin B-12, folic acid and the cognitive decline in the elderly]
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M A, Smach, S, Naffeti, B, Charfeddine, J, Ben Abdallah, L B, Othmen, A, Letaef, and K, Limem
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Vitamin B 12 ,Folic Acid ,Risk Factors ,Dietary Supplements ,Hyperhomocysteinemia ,Brain ,Humans ,Neurodegenerative Diseases ,Nutritional Physiological Phenomena ,Cognition Disorders ,Homocysteine ,Receptors, N-Methyl-D-Aspartate ,Aged - Abstract
Hyperhomocysteinemia is a risk factor for neurological diseases, but the underlying pathophysiology has not been adequately explained. Mild hyperhomocysteinemia, which is sometimes associated with a low plasma level of vitamin B9, B12 and folic acid, is responsible in the toxicity in neural cell by activating NMDA receptor. Indeed, even if vitamin supplementation has clearly proven its efficiency on lowering plasma levels of homocysteine, recent studies do not show any positive effect of vitamin therapy on cognitive function. The hypothesis that this therapy is inefficient has been recently reinforced by two randomized trials on the effects of vitamin supplementation. Several hypotheses still need to be explored: Mechanisms of homocysteine toxicity and that of total uselessness of vitamin supplementation; the possible need to complete the actual data with further, more powerful studies in order to prove the role of homocysteine in the development of neurodegenerative diseases and a clinical effect of vitamin therapy.
- Published
- 2010
7. [Hypocholesterolemia and celiac disease: about one case]
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S, Neffati, B, Charfeddine, M Ali, Smach, L, Ben Othmen, A, Ltaief, I, Brahem, H, Dridi, and K, Limem
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Celiac Disease ,Diet, Gluten-Free ,Cholesterol ,Adolescent ,Incidence ,Humans ,Female ,France ,Metabolism, Inborn Errors - Abstract
Hypocholesterolemia is a biochemical abnormality that often does not have much interest for clinicians. However its frequency varies from 2 to 5% according to the studied populations and can reveal a severe disease (cancer, sareopenia, malabsorption...). We report the observation of Miss HY, 17 year old, in whom the biological association of a hypocholesterolemie state with ferriprive anaemia revealed a celiac disease. Diagnosis was confirmed by the anatomopathologic examination and analysis of both anti-gliadine and anti-endomysium antibodies. The introduction of a strict diet without gluten allowed normalization of the biological parameters and the improvement of clinical symptomatology.
- Published
- 2009
8. [Interest of CSF beta-amyloid1-42 and t-tau protein level determinations for the diagnosis of Alzheimer's disease]
- Author
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M A, Smach, B, Charfeddine, T, Lammouchi, H, Dridi, L, Ben Othman, S, Bennamou, and K, Limem
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Aged, 80 and over ,Lewy Body Disease ,Male ,Psychiatric Status Rating Scales ,Amyloid beta-Peptides ,Dementia, Vascular ,Parkinson Disease ,tau Proteins ,Middle Aged ,Neuropsychological Tests ,Magnetic Resonance Imaging ,Sensitivity and Specificity ,Peptide Fragments ,Statistics, Nonparametric ,Diagnosis, Differential ,Diagnostic and Statistical Manual of Mental Disorders ,Alzheimer Disease ,Data Interpretation, Statistical ,Humans ,Dementia ,Female ,Biomarkers ,Aged - Abstract
Early diagnosis of Alzheimer's disease remains a tactful poser. In order to clarify the importance of beta amyloid protein dosage (Abeta1-42) and protein tau (t-tau) in such pathology, we have rigorously studied three well recruited populations that match in age: healthy controls (n = 32), Alzheimer patients (n = 87) and non Alzheimer dementia (n = 31) patients. The combination of Abeta1-42 and t-tau at baseline yielded a sensitivity of 85.29 % for detection of Alzheimer's disease and the specificity was by 96.77 % to differentiate controls. So the combination of these tow markers helps in the diagnosis of Alzheimer because of the high specificity and sensibility of this method.
- Published
- 2008
9. [Diagnostic value of serum and pleural fluid adenosine deaminase activity in tuberculous pleurisy]
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M A, Smach, A, Garouch, B, Charfeddine, A, Ben Abdelaziz, H, Dridi, B, Krayem, and K, Limem
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Adult ,Male ,Pleural Effusion ,Adolescent ,Adenosine Deaminase ,Humans ,Female ,Tuberculosis, Pleural ,Middle Aged - Abstract
The biological diagnosis of tuberculous pleurisy poses the problem of the time required to obtain results and of the sensitivity of the usual diagnostic methods. The determination of adenosine deaminase (ADA) activity has been proposed for the diagnosis of tuberculous pleural effusion and for the follow-up. In the present study, ADA in pleural effusion (p-ADA) and in serum (s-ADA) has been measured in 2 groups of patients: tuberculosis (27) and non-tuberculosis (53) patients. The upper limit of the normal values was fixed at 37 U/L. Comparing these 2 groups, we observed a specificity of 81.2% and a sensitivity of 66.6%. The PPV and the NPV were respectively 64.3% and 82.7%. We used p-ADA/s-ADA ratio for diagnosis of tuberculous pleural effusion, a threshold value of 1.8 gave a sensitivity of 82.6% and a specificity of 84.8%. Within the tuberculosis patient group, the activity of ADA decreased after the appropriate treatment initiation. Thus, the determination of ADA activity and/ or the p-ADA/s-ADA ratio, can help to recognize the tuberculosis origin of pleural effusions.
- Published
- 2005
10. [Reactive hemophagocytic syndrome: about one case]
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B, Charfeddine, S, Laradi, A, Kassab, S, Ferchichi, M, El Kissi, F, Hizem, S, Baghdadi, H, Ben Limem, and A, Miled
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Diagnosis, Differential ,Histiocytosis, Non-Langerhans-Cell ,Infant, Newborn ,Humans ,Female ,Myelography - Published
- 2003
11. 303 USE OF 20 CORE PATTERN TECHNIQUE FOR INITIAL PROSTATE BIOPSY: IMPACT ON PROSTATIC CANCER DETECTION
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R. Khiari, J. Ghorbel, B. Charfeddine, M. Ben Ali, S. Ghozzi, M. Dridi, H. Khouni, J. Maarouf, A. Gammoudi, and N. Ben Rais
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PCA3 ,Core (optical fiber) ,Oncology ,medicine.medical_specialty ,Prostate biopsy ,medicine.diagnostic_test ,business.industry ,Urology ,Internal medicine ,medicine ,Cancer detection ,Radiology ,business - Published
- 2011
12. 279 Impact of treatment on the kinetics of the PSA in chronic prostatitis: Prospective randomized controlled study: Ciprofloxacin + Piroxicam vs Ciprofloxacin
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J. Ghorbel, J. Maarouf, M. Dridi, R. Khiari, H. Khouni, S. Ghozzi, B. Charfeddine, N. Benrais, and A. Laajili
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medicine.medical_specialty ,business.industry ,Urology ,Prostatitis ,medicine.disease ,Piroxicam ,Gastroenterology ,law.invention ,Surgery ,Ciprofloxacin ,Randomized controlled trial ,law ,Internal medicine ,medicine ,business ,medicine.drug - Published
- 2012
13. Neuroprotective and anti-amnesic effects of Laurus Nobilis essential oil against scopolamine-induced memory deficits in mice brain.
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Smach MA, Hafsa J, Ben Abdallah J, Charfeddine B, and Limem K
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- Mice, Animals, Scopolamine toxicity, Acetylcholinesterase metabolism, Eucalyptol pharmacology, Eucalyptol therapeutic use, Memory Disorders chemically induced, Memory Disorders drug therapy, Memory Disorders metabolism, Oxidative Stress, Maze Learning, Brain, Cholinergic Agents pharmacology, Laurus metabolism, Oils, Volatile adverse effects, Neuroprotective Agents adverse effects
- Abstract
Ethnopharmacological Relevance: Laurus nobilis L. (Lauraceae family) has been widely used in traditional Tunisian medicine for the treatment of different health problems such as rheumatism and some neurological disorders., Aim: In this study, the essential oil obtained from Laurus nobilis L. species from Tunisia (LEO) was studied for its chemical composition and anti-amnesic activities on memory impairment caused by scopolamine injection in mice. The major compounds of LEO oil, 1,8-cineole and, α-terpinyl acetate were docked with AChE (Acetylcholinesterase), using Autodock Vina and Discovery Studio visualizer software., Materials and Methods: The Morris water maze (MWM) tests and the Y maze were used to assess the anti-amnesic effects of LEO in mice with scopolamine-induced memory impairments. In brain tissues, the levels of biomarkers, enzyme activity, and protein expression related to the cholinergic system were measured., Results: Chronic administration of scopolamine led to a significant decline in cognitive performance in both the Morris Water Maze (MWM) and Y maze tests, accompanied by pronounced oxidative damage and a significant increase in acetylcholinesterase activity compared to the other groups. However, compared to the scopolamine group, treatment with LEO (100 mg/kg) significantly enhanced cognitive function and ameliorated the oxidative damage (p < 0.05 versus scopolamine) CONCLUSION: This study demonstrates the beneficial effect of LEO on scopolamine-induced dementia in mice, potentially achieved through the modulation of cholinergic activity and antioxidant properties. The docking analysis of the major compounds, 1,8-cineole and α-terpinyl acetate, further substantiates their potential as memory enhancers., Competing Interests: Declaration of competing interest The authors of this article declare that there are no conflicts of interest to disclose. The research and content presented in this article are based on our impartial and unbiased evaluation of the data. We have followed ethical guidelines and good scientific practices throughout the research process., (Published by Elsevier B.V.)
- Published
- 2024
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14. Evaluation of the diagnostic performance of Alpha-1-Antitrypsin in early detection of hepatocellular carcinoma.
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Sultan MQ, Charfeddine B, and Hussain Al-Salih AR
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- Humans, alpha-Fetoproteins, Early Detection of Cancer, ROC Curve, DNA, Biomarkers, Tumor genetics, Carcinoma, Hepatocellular diagnosis, Carcinoma, Hepatocellular genetics, Carcinoma, Hepatocellular pathology, Liver Neoplasms diagnosis, Liver Neoplasms genetics, Liver Neoplasms pathology
- Abstract
Hepatocellular carcinoma (HCC) constitutes one of the most frequent cancer types and accounting the vast majority of tumour-related fatalities worldwide. HCC is remain related to a bad prognosis in patients with an advanced disease stage. This study was conducted to evaluate the relationship between A1AT concentration, A1AT gene promoter methylation, and A1AT genotype variation, and HCC risk. In this case-control research, we investigated A1AT levels in plasma as a diagnostic biomarker for the earlier detection of HCC in 100 patient samples. We also checked DNA promoter methylation of the A1AT gene, and genotypes in all the studied groups. The levels of AFP and A1AT in plasma were determined using ELISA and nephelometric techniques, respectively. The genomic DNA extracted from blood samples has been examined for S and Z genotypes using the PCR-RFLP technique, as well as gene A1AT promoter methylation was assessed by methylation specific-PCR assay.The plasma data analysis showed that there was a significant difference between HCC and healthy control samples regarding the level of AFP and A1AT. The range of plasma A1AT concentration was 166.6±27.28g/L in patients and 129.8±15.87g/L in controls (p <0.001). A1AT concentration was also associated with progressive tumour stages. Moreover, the roc curve stated that A1AT concentration is better in sensitivity than using AFP in early detection of HCC cancer patients as A1AT concentration at 135mg/L, had a sensitivity of 99% and a specificity of 79% for distinguishing cancer patients from healthy individuals. We concluded that the plasma A1AT concentration has higher sensitivity than AFP for early detection of HCC.
- Published
- 2023
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15. Chemical characteristics and cancer risk assessment of smokeless tobacco used in Tunisia (neffa).
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Guezguez F, Abdelwaheb M, Anane I, Rekik S, Saguem S, Charfeddine B, and Rouatbi S
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- Cadmium analysis, Carcinogens analysis, Carcinogens isolation & purification, Chromatography, High Pressure Liquid, Humans, Lead analysis, Polycyclic Aromatic Hydrocarbons isolation & purification, Risk Assessment, Spectrophotometry, Atomic, Tobacco Use adverse effects, Tobacco, Smokeless adverse effects, Tunisia, Neoplasms etiology, Polycyclic Aromatic Hydrocarbons analysis, Tobacco, Smokeless analysis
- Abstract
Introduction: neffa, a form of air-dried smokeless tobacco used in North Africa, is spuriously perceived as a lower risk alternative to smoking. The objective of this study was to provide information on some harmful constituents of neffa and to use them for cancer risk assessment., Methods: a high-performance liquid chromatography method coupled with fluorescence detector was used to determine polycyclic aromatic hydrocarbons (PAHs) in one sample of neffa. An atomic absorption spectrometry was performed to determine the concentrations of lead and cadmium in three samples of neffa. The levels of toxicants found in neffa were used to assess for lifetime cancer risk as advocated by the US Environment Protection Agency., Results: the determination of PAHs in neffa allowed the identification of phenanthrene and anthracene. However, the higher molecular weight PAHs such as Benzo(a)Pyrene B(a)P were not detected. The concentrations of cadmium and lead varied between 1.3 to 2.8µg/g and 1.7 to 4.6µg/g respectively. Cancer risk for cadmium and lead varied between 4.2E-03 to 9.3E-03 and 2.5E-06 to 6.4E-06 respectively. Cancer risk for Cd exceeded the range of 10E-04 to 10E-06 of an acceptable risk., Conclusion: neffa is not a healthy alternative for overcoming smoking addiction. It contains mineral and organic pulmonary toxicants. This study could serve as a scientific basis to inform consumers about the products´ toxicity and help them to quit smokeless tobacco (SLT) use., Competing Interests: The authors declare no competing interests., (Copyright: Chukwukasi Wilson Kassy et al.)
- Published
- 2021
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16. Maillard Reaction Products and Phenolic Compounds from Roasted Peanut Flour Extracts Prevent Scopolamine-Induced Amnesia Via Cholinergic Modulation and Antioxidative Effects in Mice.
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Smach MA, Zarrouk A, Hafsa J, Gaffrej H, Ben Abdallah J, Charfeddine B, and Limem K
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- Acetylcholinesterase, Amnesia chemically induced, Amnesia drug therapy, Animals, Arachis, Cholinergic Agents, Flour, Glycation End Products, Advanced, Maze Learning, Mice, Plant Extracts, Antioxidants, Scopolamine toxicity
- Abstract
Research on the beneficial effects of Maillard reaction products (MRPs) and phenolic compounds derived from roasted peanut flour on the nervous system remains insufficient. This study aimed to evaluate the effect of a 28-day oral administration of defatted peanut extract rich in MPRs and polyphenolic compounds on the cognitive impairments and oxidative injury induced by scopolamine in a mouse model. Light and dark extracts from peanut flour were prepared by heating peanuts at 187°C for two different times (8.6 and 12.7 min) and defatted using soxhlet apparatus. The mice were orally pretreated with either roasted defatted peanuts extracts (100 mg/kg) or donepezil (3 mg/kg) for 21 days. On day 19 and until day 28, mice were injected subcutaneously with water or scopolamine (1 mg/kg body weight) 15 min after roasted defatted peanuts extracts/water feeding. Mice were subsequently subjected to a battery of behavioral tests including open field locomotor activity assay, and Morris water maze test. Brain tissues were collected to measure acetylcholine, acetylcholinesterase, and oxidative parameters (glutathione and malondialdehyde). Roasted defatted peanuts (light and dark) (100 mg/kg) treatment significantly ameliorated cognitive performance and reversed the oxidative damage when compared with the scopolamine group. These data demonstrate the defatted peanuts extracts exert potent anti-amnesic effects via the modulation of cholinergic and antioxidant activities.
- Published
- 2021
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17. Arthrophytum scoparium Extract Improves Memory Impairment and Affects Acetylcholinesterase Activity In Mice Brain.
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Smach MA, Hafsa J, Charfeddine B, Dridi H, Limem K, and Jihene BA
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- Animals, Brain enzymology, Brain metabolism, Disease Models, Animal, Galactose, Male, Malondialdehyde metabolism, Maze Learning drug effects, Memory Disorders enzymology, Mice, Oxidative Stress drug effects, Plant Extracts isolation & purification, Random Allocation, Acetylcholinesterase metabolism, Amaranthaceae chemistry, Brain drug effects, Memory Disorders drug therapy, Plant Extracts therapeutic use
- Abstract
Background: Arthrophytum scoparium (Pomel) Iljin (Amaranthaceae family) has been widely used in traditional Tunisian medicine to treat many disorders such as migraine, headache, and neurological disorders. This study investigates the effect of Arthrophytum scoparium Aqueous Extract (ASAE) on cognitive impairments and oxidative injury induced by galactose (10%) in a mouse model., Materials and Methods: The mice were divided randomly into 4 experimental groups, including the control group (saline water 9 ‰), Galactose group, Scop group (300 mg/kg/d), and Scop+Gal group (300 mg/kg/d). Mice received the corresponding solutions by intraperitoneal injection (i.p.) for 7 days before the Y-maze active tests. Galactose 10% was given to all groups except control and Scop groups, 30 min before the trial. Levels of Acetylcholinesterase Activity (AChE), Ascorbic Acid (AA), Gluthatione (GSH) and Malondialdehyde (MDA) in mice brains were examined., Results: Chronic administration of galactose significantly impaired cognitive performance in Y maze, caused marked oxidative damages and a significant increase in the acetylcholinesterase activity as compared to other groups. On the contrary, ASAE (300 mg/kg) treatment suppressed galactoseinduced oxidative damage by ameliorating the increased levels of GSH and AA. Moreover, ASAE treatment reduced brain AChE activities in the galactose-induced model., Conclusion: These findings suggest that ASAE exerts potent anti-amnesic effects via the modulation of cholinergic and antioxidant activities. The observed pharmacological activities should be further evaluated by detailed experimental studies and validated by clinical trials., (Copyright© Bentham Science Publishers; For any queries, please email at epub@benthamscience.net.)
- Published
- 2020
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18. Effects of Carpobrotus edulis Extract on Oxidative Stress and 158N Oligodendrocyte Death.
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Zarrouk A, Smach MA, Hafsa J, Sghaier R, Majdoub H, Hammami M, and Charfeddine B
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- Animals, Cell Line, Drug Evaluation, Preclinical, Hydroxycholesterols, Mice, Neuroprotection, Oligodendroglia metabolism, Phytotherapy, Plant Extracts therapeutic use, Aizoaceae, Neurodegenerative Diseases prevention & control, Oligodendroglia drug effects, Oxidative Stress drug effects, Plant Extracts pharmacology
- Abstract
Objective: Age-related diseases, including neurodegenerative diseases, are associated with oxidative stress and lipid peroxidation, and increase the levels of cholesterol auto-oxidation products such as 7β-hydroxycholesterol (7β-OHC). Thus, it is imperative to identify agents that can prevent 7β-OHC-induced side-effects., Methods: We evaluated the potential protective effects of Carpobrotus edulis ethanol-water extract (EWe) on murine oligodendrocytes (158N) cultured in the absence or presence of 7β-OHC (20 μg/mL, 24 h). The cells were incubated with EWe (20-200 µg/mL) 2 h before 7β-OHC treatment. Mitochondrial activity and cell growth were evaluated with the MTT assay. Photometric methods were used to analyze antioxidant enzyme [catalase (CAT) and glutathione peroxidase (GPx)] activities and the generation of lipid and protein oxidation products [malondialdehyde (MDA), conjugated diene (CD), and carbonylated proteins (CPs)]., Results: Treatment with 7β-OHC induced cell death and oxidative stress (reflected by alteration in CAT and SOD activities). Overproduction of lipid peroxidation products (MDA and CDs) and CPs was also reported. The cytotoxic effects associated with 7β-OHC were attenuated by 160 μg/mL of EWe of C. edulis. Cell death induced by 7β-OHC treatment was ameliorated, GPx and CAT activities were restored to normal, and MDA, CD, and CP levels were reduced following C. edulis extract treatment., Conclusion: These data demonstrate the protective activities of C. edulis EWe against 7β-OHC-induced disequilibrium in the redox status of 158N cells, indicative of the potential role of this plant extract in the prevention of neurodegenerative diseases., (Copyright © 2019 The Editorial Board of Biomedical and Environmental Sciences. Published by China CDC. All rights reserved.)
- Published
- 2019
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19. 7β-hydroxycholesterol-induced cell death, oxidative stress, and fatty acid metabolism dysfunctions attenuated with sea urchin egg oil.
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Zarrouk A, Ben Salem Y, Hafsa J, Sghaier R, Charfeddine B, Limem K, Hammami M, and Majdoub H
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- Animals, Gas Chromatography-Mass Spectrometry, Glutathione Peroxidase metabolism, Lipid Peroxidation drug effects, Magnetic Resonance Spectroscopy, Sea Urchins, Superoxide Dismutase metabolism, Cell Death drug effects, Fatty Acids metabolism, Hydroxycholesterols pharmacology, Oils pharmacology, Ovum metabolism, Oxidative Stress drug effects
- Abstract
Some oxysterols resulting either from enzymatic oxidation or autoxidation of cholesterol are associated with age-related diseases including neurodegenerative diseases. Among these oxysterols, 7β-hydroxycholesterol (7β-OHC) is often found at increased levels in patients. It is therefore important to identify molecules or mixtures of molecules to prevent 7β-OHC-induced side effects. Consequently, murine oligodendrocytes (158N) were cultured in the absence or presence of 7β-OHC (20 μg/mL, 24 h) with or without a natural oil extracted from sea urchin (Paracentrotus lividus) eggs known for its biological activity. Firstly, the chemical composition of this oil was determined using
31 P NMR and GC-MS. Secondly, this oil was used to reduce 7β-OHC-induced side effects. To this end, the oil (160 μg/mL) was added to the culture medium of 158N cells 2 h before 7β-OHC. The effects of 7β-OHC with or without the oil on cell viability were studied with the MTT test. Photometric methods were used to analyze antioxidant enzyme activities, superoxide dismutase (SOD) and glutathione peroxidase (GPx), as well as the generation of lipid peroxidation products (malondialdehyde (MDA), conjugated dienes (CDs)) and protein oxidation product (carbonylated proteins (CPs)). Gas chromatography was used to determine the fatty acid profile. With 7β-OHC, an induction of cell death associated with oxidative stress (alteration of GPx and SOD activities) was observed; an overproduction of lipid peroxidation products (MDA and CDs) and CPs was also revealed. Sea urchin egg oil attenuated 7β-OHC-induced cytotoxicity: 7β-OHC-induced cell death was reduced, GPx and SOD activities were normalized, and lower levels of MDA, CDs and CPs were produced. In addition, whereas a disturbed fatty acid profile was observed with 7β-OHC, similar fatty acid profiles were found in control cells and in cells cultured with 7β-OHC associated with sea urchin egg oil. These data demonstrate the protective activities of sea urchin egg oil against 7β-OHC-induced side effects on 158N cells, supporting the concept that this oil may have benefits in the prevention of neurodegenerative diseases., (Copyright © 2018 Elsevier B.V. and Société Française de Biochimie et Biologie Moléculaire (SFBBM). All rights reserved.)- Published
- 2018
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20. Characterization, antioxidant and antiglycation properties of polysaccharides extracted from the medicinal halophyte Carpobrotus edulis L.
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Hafsa J, Hammi KM, Cerf DL, Limem K, Majdoub H, and Charfeddine B
- Subjects
- Antioxidants pharmacology, Free Radical Scavengers, Glucose chemistry, Hexuronic Acids chemistry, Hypoglycemic Agents chemistry, Hypoglycemic Agents pharmacology, Lipid Peroxidation drug effects, Magnetic Resonance Spectroscopy, Monosaccharides chemistry, Plant Extracts pharmacology, Polysaccharides isolation & purification, Polysaccharides pharmacology, Serum Albumin, Bovine chemistry, Spectroscopy, Fourier Transform Infrared, Sugars chemistry, Uronic Acids chemistry, Water chemistry, Aizoaceae chemistry, Antioxidants chemistry, Plant Extracts chemistry, Polysaccharides chemistry
- Abstract
In this study, Box-Behnken design was used to optimize the ultrasonic extraction of Carpobrotus edulis polysaccharides (CEP), and the effect of time, extraction temperature and water to material ratio was evaluated. Optimum conditions were 1.77h, 78.0°C and 33.04mL/g to improved CEP yield (7.84%), which is in good agreement with the predicted yield 7.77%. Then, the physico-chemical, antioxidant and antiglycation properties of optimized CEP were studied, and the total sugar and galacturonic acid content were 89.7 and 63.2%, respectively. The composition of neutral monosaccharide was arabinose, xylose, rhamnose and mannose in the molar percentage of 71.84, 14.80, 8.57, and 4.79%, respectively. In addition, (
1 H, and13 C) NMR and FTIR analyses confirmed the presence of uronic acids in the free and methyl ester forms with a degree of esterification of 31.27%. Therefore, this finding showed that CEP is a low methoxyl pectic polysaccharide, with an average molecular weight about 65,000g/mol. Finally, the results indicated that CEP presents strong antioxidant activities in vitro (DPPH, chelating ability and reducing power), and significantly inhibits lipid peroxidation and the formation of fluorescent advanced glycation end products in glucose-BSA system model., (Copyright © 2017 Elsevier B.V. All rights reserved.)- Published
- 2018
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21. Effect of ultrasonic degradation of hyaluronic acid extracted from rooster comb on antioxidant and antiglycation activities.
- Author
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Hafsa J, Chaouch MA, Charfeddine B, Rihouey C, Limem K, Le Cerf D, Rouatbi S, and Majdoub H
- Subjects
- Animals, Antioxidants chemistry, Antioxidants isolation & purification, Biphenyl Compounds chemistry, Drug Stability, Glucuronic Acid isolation & purification, Glycosylation, Hyaluronic Acid chemistry, Hyaluronic Acid isolation & purification, Hypoglycemic Agents chemistry, Hypoglycemic Agents isolation & purification, Lipid Peroxidation drug effects, Male, Molecular Structure, Molecular Weight, Nitric Oxide chemistry, Picrates chemistry, Spectrophotometry, Ultraviolet, Spectroscopy, Fourier Transform Infrared, Thiobarbituric Acid Reactive Substances chemistry, Tissue Extracts chemistry, Tissue Extracts isolation & purification, Viscosity, Antioxidants pharmacology, Chickens metabolism, Comb and Wattles metabolism, Glycation End Products, Advanced metabolism, Hyaluronic Acid pharmacology, Hypoglycemic Agents pharmacology, Protein Processing, Post-Translational drug effects, Serum Albumin, Bovine metabolism, Tissue Extracts pharmacology, Ultrasonics
- Abstract
Content: Recently, low-molecular-weight hyaluronic acid (LMWHA) has been reported to have novel features, such as free radical scavenging activities, antioxidant activities and dietary supplements., Objective: In this study, hyaluronic acid (HA) was extracted from rooster comb and LMWHA was obtained by ultrasonic degradation in order to assess their antioxidant and antiglycation activities., Materials and Methods: Molecular weight (Mw) and the content of glucuronic acid (GlcA) were used as the index for comparison of the effect of ultrasonic treatment. The effects on the structure were determined by ultraviolet (UV) spectra and Fourier transform infrared spectra (FTIR). The antioxidant activity was determined by three analytical assays (DPPH, NO and TBARS), and the inhibitory effect against glycated-BSA was also assessed., Results: The GlcA content of HA and LMWHA was estimated at about 48.6% and 47.3%, respectively. The results demonstrate that ultrasonic irradiation decreases the Mw (1090-181 kDa) and intrinsic viscosity (1550-473 mL/g), which indicate the cleavage of the glycosidic bonds. The FTIR and UV spectra did not significantly change before and after degradation. The IC
50 value of HA and LWMHA was 1.43, 0.76 and 0.36 mg/mL and 1.20, 0.89 and 0.17 mg/mL toward DPPH, NO and TBARS, respectively. Likewise LMWHA exhibited significant inhibitory effects on the AGEs formation than HA., Discussion and Conclusion: The results demonstrated that the ultrasonic irradiation did not damage and change the chemical structure of HA after degradation; furthermore, decreasing Mw and viscosity of LMWHA after degradation may enhance the antioxidant and antiglycation activity.- Published
- 2017
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22. Inhibition of protein glycation, antioxidant and antiproliferative activities of Carpobrotus edulis extracts.
- Author
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Hafsa J, Hammi KM, Khedher MRB, Smach MA, Charfeddine B, Limem K, and Majdoub H
- Subjects
- Antioxidants isolation & purification, Cell Proliferation physiology, Cell Survival drug effects, Cell Survival physiology, Dose-Response Relationship, Drug, Glycation End Products, Advanced physiology, HCT116 Cells, Humans, Plant Extracts isolation & purification, Antioxidants pharmacology, Cell Proliferation drug effects, Glycation End Products, Advanced antagonists & inhibitors, Plant Extracts pharmacology, Plants, Medicinal
- Abstract
Carpobrotus edulis is an important South African medicinal plants used as a food and therapeutic agent in traditional medicine. The aim of this study was to determine the phytochemical content, antioxidant, antiglycation and cytotoxic effect against Human Colon Cancer Cell Line (HCT-116) of aqueous and ethanol-water (1:1v/v) extracts of Carpobrotus edulis.The content of total phenolics and flavonoids in aqueous and ethanol-water extract were 151.99μg and 66.35μg gallic acid equivalents/mg of dry extract, and 38.84μg and 21.96μg quercetin/mg of dry extract, respectively. Furthermore, phenolic compositions analysis indicated the presence of seven majority compounds including sinapic acid, ferulic acid, luteolin7-o-glucoside, hyperoside, isoquercitrin, ellagic acid and isorhamnetin 3-O-rutinoside. The ethanol-water extract (100-1000μg/mL) showed better antioxidant activity than aqueous extract. Furthermore, Carpobrotus edulis extracts, especially ethanol-water extract significantly inhibited the formation of fluorescent advanced glycation end products, prevented oxidation-induced protein damage and exhibited a cytotoxic effect against HCT116 cells, with a significant decrease in cell viability after 24h of incubation. The results obtained suggest that the Carpobrotus edulis extracts could be used as an easily accessible source of natural antioxidants and as potential phytochemicals against protein glycation and colon cancer., (Copyright © 2016 Elsevier Masson SAS. All rights reserved.)
- Published
- 2016
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23. Antioxidant and antimicrobial proprieties of chitin and chitosan extracted from Parapenaeus Longirostris shrimp shell waste.
- Author
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Hafsa J, Smach MA, Charfeddine B, Limem K, Majdoub H, and Rouatbi S
- Subjects
- Animals, Bacteria drug effects, Chitin chemistry, Chitin isolation & purification, Chitosan chemistry, Chitosan isolation & purification, Fungi drug effects, Lipid Peroxidation drug effects, Microbial Sensitivity Tests, Anti-Infective Agents pharmacology, Antioxidants pharmacology, Chitin pharmacology, Chitosan pharmacology, Penaeidae chemistry
- Abstract
Introduction: Chitosan, the linear polymer, is produced by alkali deacetylation of chitin (CHI). Recently chitin and chitosan were attracted marked interest due to their biocompatibility, biodegradability and non-toxicity., Materials and Methods: In this study, chitin was extracted from shrimp shell (Parapenaeus longirostris) and chitosan was deacetylated by classical and ultrasound-assisted method. The identification of functional groups and the determination of degree of deacetylation of chitin (CHI), classical deacetylated chitosan (CDC) and ultrasound-assisted deacetylated chitosan (UDC) were carried through Fourier Transform-Infrared Spectroscopy. Their antimicrobial and antioxidant activity were also investigated., Results: The degree of deacetylation of CHI, CDC and UDC is 33.64%, 73.68% and 83.55%, respectively. Results showed that CHI, CDC and UDC exhibited a good antimicrobial activity against (S. aureus, E. coli, P. aeruginosa, K. pneumonia) and (C. albicans and C. parapsilosis). The scavenging ability of CHI, CDC and UDC on 1,1-diphenyl-2-picrylhydrazyl radicals is ranging from 4.71% to 21.25%, 11.45% to 32.78% and 18.27% to 44.17%, respectively, at the concentrations of 0.25 to 1mg/mL. The inhibition of lipid peroxidation with thiobarbituric acid-reacting substances is ranging from 11.7% to 51.63%, 17.24% to 63.52% and 29.31% to 77.39%, respectively, at varying concentrations of 0.25 to 1mg/mL., Conclusion: The effectiveness of CHI, CDC and UDC is correlated with their degree of deacetylation. The results indicate the possibility of exploiting chitin and chitosan as antimicrobial agent., (Copyright © 2015 Académie Nationale de Pharmacie. Published by Elsevier Masson SAS. All rights reserved.)
- Published
- 2016
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24. Effects of sage extract on memory performance in mice and acetylcholinesterase activity.
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Smach MA, Hafsa J, Charfeddine B, Dridi H, and Limem K
- Subjects
- Animals, Male, Medicine, Traditional, Mice, Cholinergic Antagonists pharmacology, Cholinesterase Inhibitors pharmacology, Memory drug effects, Plant Extracts pharmacology, Salvia officinalis chemistry
- Abstract
Introduction: Salvia officinalis L. (sage) has been widely used in the Tunisian traditional medicine for the treatment of digestive system and memory disorders., Objectives: This work aims to investigate the anti-amnesiac and antioxidant activity of aqueous extract of S. officinalis (ASOE) in mice., Materials and Methods: Evaluation of memory functions in rodents was assessed by Y-maze active test after an initial acquisition phase., Results: Results showed that the S. officinalis aqueous extract had high total phenolic contents (6.2mg gallic E/g extract) and flavonoids (2.44mg catechin E/g extract). It exhibited an interesting scavenging activity (IC50=14.5μg/ml). The mice treated with 300mg/kg body weight for 7 days exhibited a significant decrease in avoidance and discrimination errors during a retention trial compared to control group (P<0.05). Acetylcholinesterase (AChE) activity significantly decreased in mice treated with the extract of sage., Conclusion: These findings suggested that aqueous extract of sage leaves possess a mnemonic effect on adult male mice, confirming their use in traditional medicine., (Copyright © 2015 Elsevier Masson SAS. All rights reserved.)
- Published
- 2015
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25. IL-10R1 (Ser138Gly) functional polymorphism is associated with acute myocardial infarction in Tunisian patients.
- Author
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Khlifi L, Chabchoub E, Letaief A, Othmen LB, Smach MA, Charfeddine B, Zemni R, and Khalifa L
- Subjects
- Biomarkers, Case-Control Studies, Female, Humans, Male, Middle Aged, Myocardial Infarction blood, Tunisia, White People, Genetic Predisposition to Disease, Interleukin-10 Receptor alpha Subunit genetics, Myocardial Infarction genetics
- Published
- 2014
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26. [Homocysteine, vitamin B-12, folic acid and the cognitive decline in the elderly].
- Author
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Smach MA, Naffeti S, Charfeddine B, Ben Abdallah J, Othmen LB, Letaef A, and Limem K
- Subjects
- Aged, Brain metabolism, Dietary Supplements, Humans, Hyperhomocysteinemia complications, Hyperhomocysteinemia drug therapy, Neurodegenerative Diseases drug therapy, Neurodegenerative Diseases etiology, Nutritional Physiological Phenomena, Receptors, N-Methyl-D-Aspartate physiology, Risk Factors, Cognition Disorders drug therapy, Cognition Disorders etiology, Folic Acid administration & dosage, Homocysteine physiology, Vitamin B 12 administration & dosage
- Abstract
Hyperhomocysteinemia is a risk factor for neurological diseases, but the underlying pathophysiology has not been adequately explained. Mild hyperhomocysteinemia, which is sometimes associated with a low plasma level of vitamin B9, B12 and folic acid, is responsible in the toxicity in neural cell by activating NMDA receptor. Indeed, even if vitamin supplementation has clearly proven its efficiency on lowering plasma levels of homocysteine, recent studies do not show any positive effect of vitamin therapy on cognitive function. The hypothesis that this therapy is inefficient has been recently reinforced by two randomized trials on the effects of vitamin supplementation. Several hypotheses still need to be explored: Mechanisms of homocysteine toxicity and that of total uselessness of vitamin supplementation; the possible need to complete the actual data with further, more powerful studies in order to prove the role of homocysteine in the development of neurodegenerative diseases and a clinical effect of vitamin therapy., (Copyright © 2012 Elsevier Masson SAS. All rights reserved.)
- Published
- 2013
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27. [Comparative study of C-reactive protein and procalcitonin in the severity diagnosis of pyelonephritis in children].
- Author
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Bouguila J, Khalef I, Charfeddine B, Ben Rejeb M, Chatti K, Limam K, Essabbeh H, Essoussi AS, and Boughammoura L
- Subjects
- Adolescent, Calcitonin Gene-Related Peptide, Child, Child, Preschool, Female, Humans, Infant, Infant, Newborn, Male, Predictive Value of Tests, Pyelonephritis blood, Radionuclide Imaging, Sensitivity and Specificity, Severity of Illness Index, Succimer, C-Reactive Protein analysis, Calcitonin blood, Protein Precursors blood, Pyelonephritis diagnosis
- Abstract
Objective: The aim of this study is to compare two biologic parameters; C-reactive protein (CRP) and procalcitonin (PCT) in the detection of acute renal lesions assessed by DMSA scintigraphy in the urinary tract infection in child., Design: In a prospective study, serum PCT, CRP and leukocyte counts were measured for children admitted, between January and December 2010, with a first episode of febrile urinary tract infection., Results: Seventy-five children were enrolled in the study. Thirty-three patients had renal lesions (group A) and 42 had a normal DMSA scintigraphy (group B). The mean PCT level was significantly higher in group A than in group B (8.81 ng/mL versus 1.7 ng/mL, P=0.01). In this study, using receiver operating characteristic (ROC) curve, we identified that the optimal cut-off value with ideal sensitivity and specificity for PCT in detection of renal lesions was 0.76 ng/mL and for CRP, it was 70 mg/L. The sensitivity, the negative predictive value and the indice of Youden of the cut-off value of PCT were significantly higher than CRP (82% versus 70%; 84% versus 70% and 0.58 versus 0.25)., Conclusions: This study confirmed that the serum PCT level was more sensitive and specific than the CRP in the detection of renal lesions in the first urinary tract infection in child., (Copyright © 2013 Elsevier Masson SAS. All rights reserved.)
- Published
- 2013
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28. [Biological assay for galactose-1 phosphate measurement application in subjects with galactosemia].
- Author
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Braham I, Charfeddine B, Ben Othmene L, Neffati S, Mtar A, Ben Abdallah J, Ali Smach M, Dridi H, and Limem K
- Subjects
- Blood Chemical Analysis methods, Case-Control Studies, Child, Child, Preschool, Efficiency, Endocrinology methods, Female, Galactosemias blood, Humans, Male, Predictive Value of Tests, Reproducibility of Results, Sensitivity and Specificity, Biological Assay methods, Diagnostic Techniques, Endocrine, Galactosemias diagnosis, Galactosephosphates analysis
- Abstract
Congenital galactosemia is a hereditary, autosomal recessive and metabolic disease. It is linked to an enzyme deficiency, more commonly known by the deficiency of galactose-1- phosphate uridyltransferase (GALT), which is responsible for an accumulation of galactose-1- phosphate in the blood. Clinical symptoms appear early in infancy from the second week of life. They generally manifested by some disorders within liver, kidney, eye, gastrointestinal, neurological and also with cataracts. Currently, the clinical diagnosis remains difficult hence the importance of further investigations based on effective biological assessments to highlight the disease. The diagnosis of galactosemia is made by the laboratory test. The latter includes the determination of Gal-1-P which is done by a fluorometric method spot test. This study was conducted in order to assess the repeatability, reproducibility, accuracy, and effectiveness of the techniques used. We have found the CV for a repeatability (CV = 5 %), reproducibility (CV = 4 %) which confirms the accuracy of the method proceeded in this study. This method allows us to have a degree of inaccuracy less than 1%. According to the study of the effectiveness of "spot test", we found that our technique is specific (Sp = 93 %) and sensitive (Se = 83 %).
- Published
- 2012
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29. Six-minute walk test in non-insulin-dependent diabetes mellitus patients living in Northwest Africa.
- Author
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Latiri I, Elbey R, Hcini K, Zaoui A, Charfeddine B, Maarouf MR, Tabka Z, Zbidi A, and Ben Saad H
- Abstract
Introduction: International recommendations of the exploration of non-insulin-dependent diabetes mellitus (NIDDM) are focused on deficiency and not incapacity., Aims: (1) To estimate the incapacity of NIDDM patients through the 6-minute walk test (6MWT) data. (2) To determine their 6-minute walk distance (6MWD) influencing factors (3) To compare data of NIDDM patient group (PG; n = 100) with those of two control groups (CG): CG1 (n = 174, healthy nonobese and nonsmoker); CG2 (n = 55, obese nondiabetic free from comorbidities)., Population and Methods: The anthropometric, socioeconomic, clinical, metabolic, and 6MWT data of 100 NIDDM patients (45 females) were collected., Results: Total sample means ± standard deviation of age, weight, and height were 54 ± 8 years, 81 ± 14 kg, and 1.64 ± 0.09 m. (1) Measured 6MWD (566 ± 81 m) was significantly lower than the theoretical 6MWD (90% ± 12%). The profile of the PG carrying the 6MWT, was as follows: 23% had an abnormal 6MWD; at the end of the 6MWT, 21% and 12% had, respectively, a low heart rate and a high dyspnea (>5/10), and 4% had desaturation during the walk. The estimated "cardiorespiratory and muscular chain" age (68 ± 16 years) was significantly higher than the chronological age. (2) The factors that significantly influenced the 6MWD (r(2) = 0.58) are included in the following equation: 6MWD (m) = -73.94 × gender (0, male; 1, female) - 3.25 × age (years) + 7.33 × leisure activity score - 35.57 × obesity (0, no; 1, yes) + 32.86 × socioeconomic level (0, low; 1, high) - 27.67 × cigarette use (0, no; 1, yes) + 8.89 × resting oxyhemoglobin saturation - 105.48. (3) Compared to the CGs, the PG had a significantly (P < 0.05) lower 6MWD (100%+9% and 100%+8%, respectively, for the CG1 and CG2)., Conclusion: NIDDM seems to accelerate the decline of the submaximal aerobic capacity evaluated through the 6MWD.
- Published
- 2012
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30. [Maroteaux-Lamy syndrome: a case report].
- Author
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Mtar A, Charfeddine B, Braham I, Ben Abdallah J, Neffati S, Smach MA, Bourfifa Z, Ksouri M, Dridi H, and Limem K
- Subjects
- Child, Consanguinity, Developmental Disabilities blood, Developmental Disabilities diagnosis, Growth Disorders blood, Growth Disorders diagnosis, Humans, Male, Mucopolysaccharidosis VI blood, Mucopolysaccharidosis VI metabolism, N-Acetylgalactosamine-4-Sulfatase analysis, N-Acetylgalactosamine-4-Sulfatase blood, N-Acetylgalactosamine-4-Sulfatase metabolism, Mucopolysaccharidosis VI diagnosis
- Abstract
The Maroteaux-Lamy disease, or mucopolysaccharidosis type VI is an inherited metabolic disorder severe and rare. It is caused by a deficiency of the enzyme arylsulfatase B. It is characterized by a heterogeneous clinical, radiological and genetic. We report the case of a Maroteaux-Lamy syndrome of in a child aged 7 years whose diagnosis was suspected clinically by the combination of a dysmorphic syndrome, a failure to thrive not harmonious, hepatomegaly and normal intelligence. Radiological exams have objectified dysostosis multiplex. Biochemical analysis of urine showed the abnormal presence of dermatan sulfate. The determination of leukocyte enzyme activity confirmed the diagnosis by showing arylsulfatase B deficiency. Hence the diagnosis of syndrome Maroteaux-Lamy in its mild form (type B) was selected.
- Published
- 2011
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31. [Primary distal renal tubular acidosis: a case report].
- Author
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Abdallah JB, Charfeddine B, Braham I, Neffati S, Othmen LB, Letaief A, Smach MA, Bourfifa Z, Dridi H, and Limem K
- Subjects
- Child, Preschool, Humans, Male, Acidosis, Renal Tubular diagnosis
- Abstract
The primary distal renal tubular acidosis is characterized biochemically by the inability of the kidney to produce appropriately acid urine in the presence of systemic metabolic acidosis or after acid loading (e.g. ammonium chloride). It is secondary to defective excretion of H(+) by the cells of the collecting duct. We report the observation of the child MC, 4-year-old, for whom the association of polyuria-polydipsia syndrome, a failure to thrive, nephrolithiasis, hypercalciuria, and especially a high urine pH in the presence of metabolic acidosis did evoke diagnosis of distal renal tubular acidosis. An urine acidification test with ammonium chloride was performed, the urinary pH was always higher than 5.5, thus confirming the diagnosis.
- Published
- 2011
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32. Folate and homocysteine in the cerebrospinal fluid of patients with Alzheimer's disease or dementia: a case control study.
- Author
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Smach MA, Jacob N, Golmard JL, Charfeddine B, Lammouchi T, Ben Othman L, Dridi H, Bennamou S, and Limem K
- Subjects
- Aged, Aged, 80 and over, Case-Control Studies, Female, Homocysteine blood, Humans, Male, Middle Aged, Retrospective Studies, Statistics, Nonparametric, Tunisia, Alzheimer Disease cerebrospinal fluid, Dementia cerebrospinal fluid, Folic Acid cerebrospinal fluid, Homocysteine cerebrospinal fluid
- Abstract
Background: Amyloid deposition in the brain is an early event in Alzheimer's disease (AD), but a dysfunction of the blood-brain barrier or a disturbance in the metabolism of folate and homocysteine (Hcy) may affect the development of dementia. We investigated if the concentrations of folate and Hcy would be modified in cerebrospinal fluid (CSF) of clinically diagnosed AD patients., Methods: We included 70 AD patients, 33 patients with another type of dementia (nAD) and 30 age-matched control subjects. Plasma Hcy was assayed as well as Hcy, folate, Aβ1-42 and T-tau in CSF. We used ANOVAs for comparison between groups, and then pairwise comparisons by Wilcoxon tests with Bonferroni-corrected p values. Correlations were tested with the Spearman's rank test., Results: Levels of Aβ1-42, T-tau and folates in CSF were significantly different between groups, but not Hcy. In addition, the average folate in CSF was lower in AD patients compared with controls (18.7 ± 2.4 vs. 20.3 ± 1.7 nmol/l, Bonferroni-corrected p value < 0.02). There was no correlation between Aβ1-42 or T-tau and folate or Hcy in CSF, regardless of the group. In the AD group, there was a significant inverse correlation between Hcy and folate in CSF (ρ = -0.63, p < 0.0001), whereas in the nAD group, a significant correlation was found for Hcy between plasma and CSF (ρ = 0.59, p < 0.0005)., Conclusion: The concentration of folate in CSF was found to be decreased in AD patients. These findings support the hypothesis of a possible role of folate in the onset or worsening of AD., (Copyright © 2011 S. Karger AG, Basel.)
- Published
- 2011
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33. Polymorphism in apoA1 Influences High-Density Lipoprotein Cholesterol Levels but Is Not a Major Risk Factor of Alzheimer's Disease.
- Author
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Smach MA, Edziri H, Charfeddine B, Ben Othman L, Lammouchi T, Ltaief A, Nafati S, Dridi H, Bennamou S, and Limem K
- Abstract
Background: Apolipoprotein A1 (apoA1) is the major apolipoprotein constituent of the high-density lipoprotein (HDL) and is involved in reverse cholesterol transport. Variation in the apoA1 gene might influence the function of the protein and, thus, brain cholesterol metabolism, leading to an increased risk for Alzheimer's disease (AD)., Aim: In the current report, we investigated the role of the functional apoA1 polymorphism (-75 G/A) as a genetic risk factor for AD in a Tunisian population., Methods: 173 AD patients and 150 healthy controls were studied., Results: No association was found between this genetic variation in apoA1 gene and the risk of AD. The presence of the (-75 G/A) A allele appeared, however, to be associated with lower levels of cerebrospinal fluid Aβ42 and HDL cholesterol levels in sera., Conclusion: Our data support the observation that apoA1 polymorphism influences cholesterol metabolism and Aβ42 deposition in the brain.
- Published
- 2011
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34. Analysis of association between bleomycin hydrolase and apolipoprotein E polymorphism in Alzheimer's disease.
- Author
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Smach MA, Charfeddine B, Lammouchi T, Othman LB, Letaief A, Nafati S, Dridi H, Bennamou S, and Limem K
- Subjects
- Aged, Aged, 80 and over, Apolipoprotein E4 genetics, Female, Genetic Carrier Screening, Humans, Male, Tunisia, Alzheimer Disease enzymology, Alzheimer Disease genetics, Apolipoproteins E genetics, Cysteine Endopeptidases genetics, Genetic Predisposition to Disease genetics, Polymorphism, Genetic genetics
- Abstract
Alzheimer's disease (AD) is the leading cause of dementia. Several studies indicate a possible relationship between different genes and Alzheimer's disease. To further investigate, we have analyzed the association between the bleomycin hydrolase (BLMH) and apolipoprotein E (ApoE) polymorphisms in 93 AD patients and age- and sex-matched 113 controls from the Tunisian population. The frequency of ApoE epsilon 4 allele was found to differ significantly in AD patients compared to the control [29.5% vs. 8.8 (χ (2) = 26, df = 1, p < 0.001)] leading to an increased risk of AD in subjects with this allele (OR = 3.29, 95% CI = 1.7-6.5; p = 0.001]. This risk was found to decrease from OR = 8.4, CI = 3.3-23; p < 0.001 in subjects less than 75 years old to OR = 1.2, CI = 1.031-14; p = 0.0297 in subjects 75 years and older. No association was observed between carrying the BLMH-G genotype and AD in ε4 negative or positive subjects.
- Published
- 2010
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35. [The congenital afibrinogenemia: case report].
- Author
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Brahem I, Charfeddine B, Chraiti H, Ben Abdallah J, Ben Othmen L, Neffati S, Ali Smach M, Ltaief A, Ksourri M, Dridi H, and Limem K
- Subjects
- Adolescent, Afibrinogenemia genetics, Afibrinogenemia therapy, Blood Transfusion, Female, Hemoperitoneum diagnosis, Hemoperitoneum therapy, Humans, Afibrinogenemia congenital, Hemoperitoneum genetics
- Abstract
The deficiency in factor I or fibrinogen is a largely unknown genetic disease. It is a rare condition inherited as an autosomal recessive, whose clinical events are variable, ranging from moderate to minimal bleeding or cataclysmic hemorrhage. We report a case of congenital afibrinogenemia in a 17 years-old patient hospitalized in surgical ICU for hemoperitoneum medium abundance discovered by abdominal ultrasound performed before a picture of abdominopelvic pain lasting for 24 hours. Exploration led to the diagnosis of congenital afibrinogenemia with favorable evolution with a contribution of factor deficient. Through this case we raise the problem of congenital afibrinogenemia in diagnosis and the peculiarities of its management.
- Published
- 2010
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36. -1154G/A and -2578C/A polymorphisms of the vascular endothelial growth factor gene in Tunisian Alzheimer patients in relation to beta-amyloid (1-42) and total tau protein.
- Author
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Smach MA, Charfeddine B, Othman LB, Lammouchi T, Ltaief A, Nafati S, Dridi H, Bennamou S, and Limem K
- Subjects
- Age of Onset, Aged, Alzheimer Disease metabolism, Apolipoprotein E4 genetics, Case-Control Studies, Female, Gene Frequency, Genetic Predisposition to Disease, Genome-Wide Association Study, Genotype, Humans, Male, Polymorphism, Genetic, Promoter Regions, Genetic, Tunisia, Alzheimer Disease genetics, Amyloid beta-Peptides cerebrospinal fluid, Peptide Fragments cerebrospinal fluid, Vascular Endothelial Growth Factor A genetics, tau Proteins cerebrospinal fluid
- Abstract
Recent evidences indicate that polymorphisms within the promoter region of the vascular endothelial growth factor (VEGF) gene may elevate the risk for Alzheimer's disease (AD). To further investigate, we have analyzed association between promoter polymorphisms of the VEGF gene in 93 AD patients and age and sex matched 113 controls from Tunisian population. The distribution of genotype and allele frequencies of the VEGF (-2578C/A) and (-1154G/A) polymorphisms did not differ significantly between AD and control groups (p>0.05). In the subgroup of ApoE varepsilon4 carriers, the -2578A was observed to be significantly higher in the AD patients than in the control individuals. After adjusting the data by gender, age and the ApoE varepsilon4 status using logistic regression, the -2578A allele was found to increase the risk for sporadic AD by 1.7-fold. The present study provides the evidence that the -2578A allele may be associated with the development of AD in the individuals with ApoE varepsilon4 allele. In addition, AD patients carrying the -2578A allele had lower Abeta42 (p=0.029) levels than those without this allele, particularly in subjects with ApoE varepsilon4 allele., (Copyright 2010 Elsevier Ireland Ltd. All rights reserved.)
- Published
- 2010
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37. [Hypocholesterolemia and celiac disease: about one case].
- Author
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Neffati S, Charfeddine B, Smach MA, Ben Othmen L, Ltaief A, Brahem I, Dridi H, and Limem K
- Subjects
- Adolescent, Female, France, Humans, Incidence, Metabolism, Inborn Errors epidemiology, Celiac Disease complications, Celiac Disease diet therapy, Cholesterol deficiency, Diet, Gluten-Free, Metabolism, Inborn Errors complications
- Abstract
Hypocholesterolemia is a biochemical abnormality that often does not have much interest for clinicians. However its frequency varies from 2 to 5% according to the studied populations and can reveal a severe disease (cancer, sareopenia, malabsorption...). We report the observation of Miss HY, 17 year old, in whom the biological association of a hypocholesterolemie state with ferriprive anaemia revealed a celiac disease. Diagnosis was confirmed by the anatomopathologic examination and analysis of both anti-gliadine and anti-endomysium antibodies. The introduction of a strict diet without gluten allowed normalization of the biological parameters and the improvement of clinical symptomatology.
- Published
- 2009
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38. Evaluation of cerebrospinal fluid tau/beta-amyloid(42) ratio as diagnostic markers for Alzheimer disease.
- Author
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Smach MA, Charfeddine B, Ben Othman L, Lammouchi T, Dridi H, Nafati S, Ltaief A, Bennamou S, and Limem K
- Subjects
- Aged, Aged, 80 and over, Alzheimer Disease cerebrospinal fluid, Analysis of Variance, Biomarkers cerebrospinal fluid, Chi-Square Distribution, Cognition Disorders cerebrospinal fluid, Cognition Disorders diagnosis, Diagnosis, Differential, Diagnostic and Statistical Manual of Mental Disorders, Enzyme-Linked Immunosorbent Assay, Female, Humans, Male, Middle Aged, Neuropsychological Tests, Phosphorylation, Statistics, Nonparametric, Alzheimer Disease diagnosis, Amyloid beta-Peptides cerebrospinal fluid, Peptide Fragments cerebrospinal fluid, tau Proteins cerebrospinal fluid
- Abstract
Background: Alzheimer's disease (AD) is the leading cause of dementia. Currently, no definitive diagnostic test for AD exists. Cerebrospinal fluid (CSF) concentrations of amyloid beta (Abeta1-42) peptides and total tau proteins (T-tau) may serve as biomarkers for AD., Aim: The objective of this study was to investigate the usefulness of CSF Abeta1-42 and T-tau analyses in the diagnosis of AD with Tunisians., Methods: We focused on three groups originating from Central Tunisian that matched in age (range 48-85): healthy controls (n = 53), AD patients (n = 93) and non-Alzheimer (nAD) dementia (n = 35) patients. Abeta1-42 and T-tau levels were measured in CSF by sandwich enzyme-linked immunosorbent assay., Results: The ratio of T-tau/Abeta1-42 at baseline yielded a sensitivity of 85.3% for detection of AD and the specificity was 84.8% to differentiate controls and nAD dementia., Conclusion: Our findings confirm the use of T-tau/Abeta1-42 ratio in the discrimination of AD patients from all other patients.
- Published
- 2009
- Full Text
- View/download PDF
39. [Interest of CSF beta-amyloid1-42 and t-tau protein level determinations for the diagnosis of Alzheimer's disease].
- Author
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Smach MA, Charfeddine B, Lammouchi T, Dridi H, Ben Othman L, Bennamou S, and Limem K
- Subjects
- Aged, Aged, 80 and over, Biomarkers, Data Interpretation, Statistical, Dementia cerebrospinal fluid, Dementia diagnosis, Dementia, Vascular cerebrospinal fluid, Dementia, Vascular diagnosis, Diagnosis, Differential, Diagnostic and Statistical Manual of Mental Disorders, Female, Humans, Lewy Body Disease cerebrospinal fluid, Lewy Body Disease diagnosis, Magnetic Resonance Imaging, Male, Middle Aged, Neuropsychological Tests, Parkinson Disease cerebrospinal fluid, Parkinson Disease diagnosis, Psychiatric Status Rating Scales, Sensitivity and Specificity, Statistics, Nonparametric, Alzheimer Disease cerebrospinal fluid, Alzheimer Disease diagnosis, Amyloid beta-Peptides cerebrospinal fluid, Peptide Fragments cerebrospinal fluid, tau Proteins cerebrospinal fluid
- Abstract
Early diagnosis of Alzheimer's disease remains a tactful poser. In order to clarify the importance of beta amyloid protein dosage (Abeta1-42) and protein tau (t-tau) in such pathology, we have rigorously studied three well recruited populations that match in age: healthy controls (n = 32), Alzheimer patients (n = 87) and non Alzheimer dementia (n = 31) patients. The combination of Abeta1-42 and t-tau at baseline yielded a sensitivity of 85.29 % for detection of Alzheimer's disease and the specificity was by 96.77 % to differentiate controls. So the combination of these tow markers helps in the diagnosis of Alzheimer because of the high specificity and sensibility of this method.
- Published
- 2008
- Full Text
- View/download PDF
40. CSF beta-amyloid 1-42 and tau in Tunisian patients with Alzheimer's disease: the effect of APOE epsilon4 allele.
- Author
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Smach MA, Charfeddine B, Lammouchi T, Harrabi I, Ben Othman L, Dridi H, Bennamou S, and Limem K
- Subjects
- Aged, Aged, 80 and over, Alleles, Alzheimer Disease diagnosis, Apolipoprotein E4 genetics, Apolipoproteins E genetics, Dementia diagnosis, Dementia genetics, Dementia metabolism, Enzyme-Linked Immunosorbent Assay methods, Female, Follow-Up Studies, Genotype, Humans, Male, Middle Aged, Neuropsychological Tests statistics & numerical data, Polymerase Chain Reaction, Predictive Value of Tests, Reference Values, Alzheimer Disease cerebrospinal fluid, Alzheimer Disease genetics, Amyloid beta-Peptides cerebrospinal fluid, Peptide Fragments cerebrospinal fluid, tau Proteins cerebrospinal fluid
- Abstract
Alzheimer's disease (AD) is the leading cause of dementia. Currently, no definitive diagnostic test for AD exists. An accurate, convenient and objective test to detect AD is urgently needed for efficient drug development and effective clinical use of emerging therapies. The aim of the present work is to investigate the usefulness of cerebrospinal fluid (CSF) beta-amyloid protein (Abeta1-42) and total tau protein (t-tau) analyses in the diagnosis of AD and whether apolipoprotein E (ApoE) epsilon4 allele is a factor for AD affecting Tunisian people. Abeta1-42 and t-tau levels were measured in CSF from AD patients (n=73), non-Alzheimer dementia (nAD, n=35) and healthy controls (HC, n=38) by sandwich enzyme-linked immunosorbent assay. Abeta1-42 levels were decreased and t-tau increased in AD patients. The combination of Abeta1-42 and t-tau at baseline yielded a sensitivity of 87.4% for detection of AD. The specificities were 97.3% for controls and 82.7% for other dementia. The ApoE epsilon4 allele frequency (29.5%) was significantly higher in the AD patients than in the nAD patients (17.1%) or in the control groups (9.5%). AD patients carrying ApoE epsilon4 allele had lower Abeta1-42 (p<0.001) levels than those without a epsilon4 allele. The combination of t-tau and Abeta1-42 is a robust and reliable assay that may be useful in discriminating cases at risk for AD such as ApoE epsilon4 allele carriers from nAD patients or from age-matched control subjects.
- Published
- 2008
- Full Text
- View/download PDF
41. [Diagnostic value of serum and pleural fluid adenosine deaminase activity in tuberculous pleurisy].
- Author
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Smach MA, Garouch A, Charfeddine B, Ben Abdelaziz A, Dridi H, Krayem B, and Limem K
- Subjects
- Adenosine Deaminase blood, Adolescent, Adult, Female, Humans, Male, Middle Aged, Tuberculosis, Pleural blood, Adenosine Deaminase metabolism, Pleural Effusion chemistry, Tuberculosis, Pleural diagnosis, Tuberculosis, Pleural enzymology
- Abstract
The biological diagnosis of tuberculous pleurisy poses the problem of the time required to obtain results and of the sensitivity of the usual diagnostic methods. The determination of adenosine deaminase (ADA) activity has been proposed for the diagnosis of tuberculous pleural effusion and for the follow-up. In the present study, ADA in pleural effusion (p-ADA) and in serum (s-ADA) has been measured in 2 groups of patients: tuberculosis (27) and non-tuberculosis (53) patients. The upper limit of the normal values was fixed at 37 U/L. Comparing these 2 groups, we observed a specificity of 81.2% and a sensitivity of 66.6%. The PPV and the NPV were respectively 64.3% and 82.7%. We used p-ADA/s-ADA ratio for diagnosis of tuberculous pleural effusion, a threshold value of 1.8 gave a sensitivity of 82.6% and a specificity of 84.8%. Within the tuberculosis patient group, the activity of ADA decreased after the appropriate treatment initiation. Thus, the determination of ADA activity and/ or the p-ADA/s-ADA ratio, can help to recognize the tuberculosis origin of pleural effusions.
- Published
- 2006
42. [Reactive hemophagocytic syndrome: about one case].
- Author
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Charfeddine B, Laradi S, Kassab A, Ferchichi S, El Kissi M, Hizem F, Baghdadi S, Ben Limem H, and Miled A
- Subjects
- Diagnosis, Differential, Female, Histiocytosis, Non-Langerhans-Cell diagnostic imaging, Humans, Infant, Newborn, Myelography, Histiocytosis, Non-Langerhans-Cell diagnosis
- Published
- 2003
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