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18 results on '"Azam, Fàtima Latif"'

1. Gene expression changes throughout the life cycle allow a bacterial plant pathogen to persist in diverse environmental habitats

Author

de Pedro-Jové, Roger, primary, Corral, Jordi, additional, Rocafort, Mercedes, additional, Puigvert, Marina, additional, Azam, Fàtima Latif, additional, Vandecaveye, Agustina, additional, Macho, Alberto P., additional, Balsalobre, Carlos, additional, Coll, Núria S., additional, Orellano, Elena, additional, and Valls, Marc, additional

Published
2023
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2. S8 Fig. Viability of R. solanacearum cells in soil microcosms

Author

Pedro-Jové, Roger de, Corral, Jordi, Rocafort, Mercedes, Puigvert, Marina, Azam, Fàtima Latif, Vandecaveye, Agustina, Macho, Alberto P., Balsalobre, Carlos, Coll, Núria S., Orellano, Elena G., Valls, Marc, Pedro-Jové, Roger de, Corral, Jordi, Rocafort, Mercedes, Puigvert, Marina, Azam, Fàtima Latif, Vandecaveye, Agustina, Macho, Alberto P., Balsalobre, Carlos, Coll, Núria S., Orellano, Elena G., and Valls, Marc

Published
2023

3. S6 Fig. Expression of all stress response and the type 3 secretion system (T3SS) and type 3 effector gene groups

Author

Pedro-Jové, Roger de, Corral, Jordi, Rocafort, Mercedes, Puigvert, Marina, Azam, Fàtima Latif, Vandecaveye, Agustina, Macho, Alberto P., Balsalobre, Carlos, Coll, Núria S., Orellano, Elena G., Valls, Marc, Pedro-Jové, Roger de, Corral, Jordi, Rocafort, Mercedes, Puigvert, Marina, Azam, Fàtima Latif, Vandecaveye, Agustina, Macho, Alberto P., Balsalobre, Carlos, Coll, Núria S., Orellano, Elena G., and Valls, Marc

Published
2023

4. S1 Fig. Experimental set-up and differentially expressed genes (DEGs)

Author

Pedro-Jové, Roger de, Corral, Jordi, Rocafort, Mercedes, Puigvert, Marina, Azam, Fàtima Latif, Vandecaveye, Agustina, Macho, Alberto P., Balsalobre, Carlos, Coll, Núria S., Orellano, Elena G., Valls, Marc, Pedro-Jové, Roger de, Corral, Jordi, Rocafort, Mercedes, Puigvert, Marina, Azam, Fàtima Latif, Vandecaveye, Agustina, Macho, Alberto P., Balsalobre, Carlos, Coll, Núria S., Orellano, Elena G., and Valls, Marc

Published
2023

5. S5 Fig. Induction of nitrogen metabolism genes in soil

Author

Pedro-Jové, Roger de, Corral, Jordi, Rocafort, Mercedes, Puigvert, Marina, Azam, Fàtima Latif, Vandecaveye, Agustina, Macho, Alberto P., Balsalobre, Carlos, Coll, Núria S., Orellano, Elena G., Valls, Marc, Pedro-Jové, Roger de, Corral, Jordi, Rocafort, Mercedes, Puigvert, Marina, Azam, Fàtima Latif, Vandecaveye, Agustina, Macho, Alberto P., Balsalobre, Carlos, Coll, Núria S., Orellano, Elena G., and Valls, Marc

Published
2023

6. S7 Fig. Expression of key genes associated with oxidative stress in soil and water at 3 dpi

Author

Pedro-Jové, Roger de, Corral, Jordi, Rocafort, Mercedes, Puigvert, Marina, Azam, Fàtima Latif, Vandecaveye, Agustina, Macho, Alberto P., Balsalobre, Carlos, Coll, Núria S., Orellano, Elena G., Valls, Marc, Pedro-Jové, Roger de, Corral, Jordi, Rocafort, Mercedes, Puigvert, Marina, Azam, Fàtima Latif, Vandecaveye, Agustina, Macho, Alberto P., Balsalobre, Carlos, Coll, Núria S., Orellano, Elena G., and Valls, Marc

Published
2023

7. S2 Fig. GO and KEGG enrichment analyses of the environmental conditions

Author

Pedro-Jové, Roger de, Corral, Jordi, Rocafort, Mercedes, Puigvert, Marina, Azam, Fàtima Latif, Vandecaveye, Agustina, Macho, Alberto P., Balsalobre, Carlos, Coll, Núria S., Orellano, Elena G., Valls, Marc, Pedro-Jové, Roger de, Corral, Jordi, Rocafort, Mercedes, Puigvert, Marina, Azam, Fàtima Latif, Vandecaveye, Agustina, Macho, Alberto P., Balsalobre, Carlos, Coll, Núria S., Orellano, Elena G., and Valls, Marc

Published
2023

8. S3 Fig. Time-course expression of the PhrpB::Lux reporter in strains disrupted for the different T3SS regulatory genes after resuspension in water

Author

Pedro-Jové, Roger de, Corral, Jordi, Rocafort, Mercedes, Puigvert, Marina, Azam, Fàtima Latif, Vandecaveye, Agustina, Macho, Alberto P., Balsalobre, Carlos, Coll, Núria S., Orellano, Elena G., Valls, Marc, Pedro-Jové, Roger de, Corral, Jordi, Rocafort, Mercedes, Puigvert, Marina, Azam, Fàtima Latif, Vandecaveye, Agustina, Macho, Alberto P., Balsalobre, Carlos, Coll, Núria S., Orellano, Elena G., and Valls, Marc

Published
2023

9. S4 Fig. Induction of the type 3 secretion system (T3SS) by basic pH in all natural water sources tested

Author

Pedro-Jové, Roger de, Corral, Jordi, Rocafort, Mercedes, Puigvert, Marina, Azam, Fàtima Latif, Vandecaveye, Agustina, Macho, Alberto P., Balsalobre, Carlos, Coll, Núria S., Orellano, Elena G., Valls, Marc, Pedro-Jové, Roger de, Corral, Jordi, Rocafort, Mercedes, Puigvert, Marina, Azam, Fàtima Latif, Vandecaveye, Agustina, Macho, Alberto P., Balsalobre, Carlos, Coll, Núria S., Orellano, Elena G., and Valls, Marc

Published
2023

10. Gene expression profiling of Ralstonia solanacearum in unexplored environmental niches reveals essential genes to complete its life cycle

Author

Pedro-Jové, Roger de, Corral, Jordi, Rocafort, Mercedes, Puigvert, Marina, Azam, Fàtima Latif, Vandecaveye, Agustina, Macho, Alberto P., Balsalobre, Carlos, Coll, Núria S., Orellano, Elena G., Valls, Marc, Pedro-Jové, Roger de, Corral, Jordi, Rocafort, Mercedes, Puigvert, Marina, Azam, Fàtima Latif, Vandecaveye, Agustina, Macho, Alberto P., Balsalobre, Carlos, Coll, Núria S., Orellano, Elena G., and Valls, Marc

Published
2023

11. Gene expression changes throughout the life cycle allow a bacterial plant pathogen to persist in diverse environmental habitats

Author

Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Generalitat de Catalunya, European Commission, Ministerio de Ciencia e Innovación (España), Universidad Autónoma de Barcelona, Pedro-Jové, Roger de, Corral, Jordi, Rocafort, Mercedes, Puigvert, Marina, Azam, Fàtima Latif, Vandecaveye, Agustina, Macho, Alberto P., Balsalobre, Carlos, Coll, Núria S., Orellano, Elena G., Valls, Marc, Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Generalitat de Catalunya, European Commission, Ministerio de Ciencia e Innovación (España), Universidad Autónoma de Barcelona, Pedro-Jové, Roger de, Corral, Jordi, Rocafort, Mercedes, Puigvert, Marina, Azam, Fàtima Latif, Vandecaveye, Agustina, Macho, Alberto P., Balsalobre, Carlos, Coll, Núria S., Orellano, Elena G., and Valls, Marc

Abstract

Bacterial pathogens exhibit a remarkable ability to persist and thrive in diverse ecological niches. Understanding the mechanisms enabling their transition between habitats is crucial to control dissemination and potential disease outbreaks. Here, we use Ralstonia solanacearum, the causing agent of the bacterial wilt disease, as a model to investigate pathogen adaptation to water and soil, two environments that act as bacterial reservoirs, and compare this information with gene expression in planta. Gene expression in water resembled that observed during late xylem colonization, with an intriguing induction of the type 3 secretion system (T3SS). Alkaline pH and nutrient scarcity—conditions also encountered during late infection stages–were identified as the triggers for this T3SS induction. In the soil environment, R. solanacearum upregulated stress-responses and genes for the use of alternate carbon sources, such as phenylacetate catabolism and the glyoxylate cycle, and downregulated virulence-associated genes. We proved through gain- and loss-of-function experiments that genes associated with the oxidative stress response, such as the regulator OxyR and the catalase KatG, are key for bacterial survival in soil, as their deletion cause a decrease in culturability associated with a premature induction of the viable but non culturable state (VBNC). This work identifies essential factors necessary for R. solanacearum to complete its life cycle and is the first comprehensive gene expression analysis in all environments occupied by a bacterial plant pathogen, providing valuable insights into its biology and adaptation to unexplored habitats.

Published
2023

12. S6 Table. Bacterial strains, plasmids, and oligonucleotides used in this work

Author

Pedro-Jové, Roger de, Corral, Jordi, Rocafort, Mercedes, Puigvert, Marina, Azam, Fàtima Latif, Vandecaveye, Agustina, Macho, Alberto P., Balsalobre, Carlos, Coll, Núria S., Orellano, Elena G., Valls, Marc, Pedro-Jové, Roger de, Corral, Jordi, Rocafort, Mercedes, Puigvert, Marina, Azam, Fàtima Latif, Vandecaveye, Agustina, Macho, Alberto P., Balsalobre, Carlos, Coll, Núria S., Orellano, Elena G., and Valls, Marc

Published
2023

13. S1 Dataset. DEGs in the two environmental conditions (Soil and Water) and in the three in planta conditions (Apoplast, Early xyem and Late xylem) compared to the reference rich B medium (phi)

Author

Pedro-Jové, Roger de, Corral, Jordi, Rocafort, Mercedes, Puigvert, Marina, Azam, Fàtima Latif, Vandecaveye, Agustina, Macho, Alberto P., Balsalobre, Carlos, Coll, Núria S., Orellano, Elena G., Valls, Marc, Pedro-Jové, Roger de, Corral, Jordi, Rocafort, Mercedes, Puigvert, Marina, Azam, Fàtima Latif, Vandecaveye, Agustina, Macho, Alberto P., Balsalobre, Carlos, Coll, Núria S., Orellano, Elena G., and Valls, Marc

Published
2023

14. S3 Table. List of genes correspoding to the intersections shown in the UpsetR plots (Fig 1B and 1D)

Author

Pedro-Jové, Roger de, Corral, Jordi, Rocafort, Mercedes, Puigvert, Marina, Azam, Fàtima Latif, Vandecaveye, Agustina, Macho, Alberto P., Balsalobre, Carlos, Coll, Núria S., Orellano, Elena G., Valls, Marc, Pedro-Jové, Roger de, Corral, Jordi, Rocafort, Mercedes, Puigvert, Marina, Azam, Fàtima Latif, Vandecaveye, Agustina, Macho, Alberto P., Balsalobre, Carlos, Coll, Núria S., Orellano, Elena G., and Valls, Marc

Published
2023

15. S1 Table. List of waters used in this work

Author

Pedro-Jové, Roger de, Corral, Jordi, Rocafort, Mercedes, Puigvert, Marina, Azam, Fàtima Latif, Vandecaveye, Agustina, Macho, Alberto P., Balsalobre, Carlos, Coll, Núria S., Orellano, Elena G., Valls, Marc, Pedro-Jové, Roger de, Corral, Jordi, Rocafort, Mercedes, Puigvert, Marina, Azam, Fàtima Latif, Vandecaveye, Agustina, Macho, Alberto P., Balsalobre, Carlos, Coll, Núria S., Orellano, Elena G., and Valls, Marc

Published
2023

16. S2 Table. Chemical analysis of the natural soil used in the study

Author

Pedro-Jové, Roger de, Corral, Jordi, Rocafort, Mercedes, Puigvert, Marina, Azam, Fàtima Latif, Vandecaveye, Agustina, Macho, Alberto P., Balsalobre, Carlos, Coll, Núria S., Orellano, Elena G., Valls, Marc, Pedro-Jové, Roger de, Corral, Jordi, Rocafort, Mercedes, Puigvert, Marina, Azam, Fàtima Latif, Vandecaveye, Agustina, Macho, Alberto P., Balsalobre, Carlos, Coll, Núria S., Orellano, Elena G., and Valls, Marc

Published
2023

17. S5 Table. Output tables of the KEGG and GO enrichment analysis conducted on general and exclusive up- and downregulated DEGs from water and soil conditions

Author

Pedro-Jové, Roger de, Corral, Jordi, Rocafort, Mercedes, Puigvert, Marina, Azam, Fàtima Latif, Vandecaveye, Agustina, Macho, Alberto P., Balsalobre, Carlos, Coll, Núria S., Orellano, Elena G., Valls, Marc, Pedro-Jové, Roger de, Corral, Jordi, Rocafort, Mercedes, Puigvert, Marina, Azam, Fàtima Latif, Vandecaveye, Agustina, Macho, Alberto P., Balsalobre, Carlos, Coll, Núria S., Orellano, Elena G., and Valls, Marc

Published
2023

18. S4 Table. TAG enrichment analysis summary

Author

Pedro-Jové, Roger de, Corral, Jordi, Rocafort, Mercedes, Puigvert, Marina, Azam, Fàtima Latif, Vandecaveye, Agustina, Macho, Alberto P., Balsalobre, Carlos, Coll, Núria S., Orellano, Elena G., Valls, Marc, Pedro-Jové, Roger de, Corral, Jordi, Rocafort, Mercedes, Puigvert, Marina, Azam, Fàtima Latif, Vandecaveye, Agustina, Macho, Alberto P., Balsalobre, Carlos, Coll, Núria S., Orellano, Elena G., and Valls, Marc

Published
2023

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