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Asap rokok mengandung radikal bebas yang dapat menyebabkan kondisi patologis tubuh berupa stres oksidatif baik pada perokok aktif maupun pasif. Stres oksidatif disebabkan peroksidasi lipid yang ditandai dengan peningkatan kadar MDA. Jika kondisi stress oksidatif tidak dinetralisir, akan menyebabkan kerusakan jaringan tubuh terutama paru yang terpapar langsung asap rokok. Antioksidan flavonoid jenis kuersetin yang terdapat pada kulit bawang merah dapat mencegah stres oksidatif.. Penelitian ini bertujuan untuk mengetahui korelasi dosis infusa kulit bawang merah (IKBM) terhadap kadar MDA serum pada tikus putih yang terpapar asap rokok. Sebanyak 28 tikus putih galur Wistar diberi paparan asap rokok sebanyak dua batang/hari. Dua jam sebelum pemberian asap rokok, pada kelompok P1 diberikan aquabidest 10 ml/kgBB sedangkan pada kelompok P2, P3, P4 dan P5 diberikan IKBM dosis 125 mg/kgBB/hari, 250 mg/kgBB/hari, 500 mg/kgBB/hari dan 1000 mg/kgBB/hari. Hasil penelitian menunjukkan bahwa terdapat korelasi negatif kuat (-0.716) dengan p=0.000 antara dosis IKBM dengan kadar MDA serum pada tikus yang terpapar asap rokok. Semakin besar dosis IKBM yang diberikan, semakin rendah kadar MDA serum tikus.

Background: The high case of tuberculosis which isn't followed by good detection becomes an urgency for the diagnostic developments. One of them with immunodiagnostic principle uses B-cell Ag85 complex epitope. The design of the diagnostic epitope was performed by mapping the B cell epitope used in silico studies.Objective: The purpose of this research is to analyze antigenicity, physicochemical which affect immunogenicity, and homology of B-cell Ag85 complex epitope with the strain which circulates in Indonesia. Methods: The samples used were taken from the NCBI protein bank with access numbers P9WQP3 for Ag85A, P9WQP1 for Ag85B, and P9WQN9 for Ag85C. The sequences were analyzed using IEDB (Bepipred) software as the epitope prediction, VaxiJen as antigenicity prediction, ProtParam as physicochemical properties prediction, and BLASTP NCBI as sequence alignment.Results: Twenty seven epitopes were antigenic with 0.4297 to 2.6007 scores and the molecular weight was from 619.59 Da to 3145.36 Da. This research also obtained eleven stable and hydrophilic epitopes. The alignment of 11 candidate epitopes with the strain which circulates in Indonesia, had a similarity percentage of 85.71%-100% and 3 epitopes had a more significant score.Conclusion: Three epitopes of Ag85 complex; Ag85A (212-235), Ag85B (209-237), and Ag85C (283-310), were universal antigens and can be developed into diagnostic antigens in Indonesia.

Objective: A comprehensive knowledge about BPH and hypertension is needed to reduce morbidity and mortality in patients with decreased kidney function due to the two diseases. Material & Methods: This research used the observational analytics method with cross-sectional design. This research used secondary data from medical records of BPH patients in Bina Sehat Hospital and Paru Hospital from January 1st to December 1st 2019. Results: Comparative serum creatinine test results in hypertensive BPH patients with non-hypertensive BPH patients using the Mann Whitney test, obtained p= 0.000. Based on these results it can be concluded that there are significant differences in serum creatinine between hypertensive BPH patients and non-hypertensive BPH patients. Correlation test results using the Spearman test between serum creatinine with blood pressure systole and diastole all samples obtained each p= 0.000 r= 0.399 for systolic blood pressure and p= 0.000 r= 0.337 for diastole blood pressure. Based on these results it can be concluded that there is a significant correlation between serum creatinine with systolic and diastolic blood pressure of the patient. Conclusion: Hypertension will worsen the kidney function decline in BPH patients seen from an increase in serum creatinine.

Pekerja pabrik tembakau dikelilingi oleh berbagai bahaya kesehatan kerja. Debu tembakau yang terhirup terus menerus menyebabkan akumulasi di saluran pernapasan yang menyebabkan peradangan dan menghasilkan jaringan fibrotik yang dapat mengurangi ekspansi paru-paru (restriction). Forced Vital Capacity (FVC) dalam hasil spirometri digunakan sebagai parameter untuk pola gangguan restriksi paru-paru. Penelitian ini bertujuan untuk mengetahui perbedaan FVC pada wanita menopause dan wanita pekerja pabrik tembakau usia subur. Jenis penelitian ini adalah observasional analitik dengan rancangan studi cross-sectional. Sampel penelitian ini adalah pekerja wanita menopause dan wanita usia subur yang bekerja di produksi pabrik tembakau. Pengumpulan data dilakukan wawancara dengan kuesioner, dan data FVC diperoleh dari hasil pemeriksaan spirometri. Penelitian ini menggunakan desain penelitian observasional analitik cross-sectional dan Unpaired T-test. Pengumpulan data tentang karakteristik individu dilakukan dengan kuesioner, sedangkan data FVC diperoleh dari spirometri. Hasil penelitian menunjukkan bahwa mayoritas pekerja pabrik tembakau perempuan mengalami penurunan nilai FVC dalam bentuk pembatasan paru-paru dan ada perbedaan nilai FVC yang signifikan antara wanita menopause dan wanita usia subur pada pekerja pabrik tembakau.

Penyakit tidak menular (PTM) merupakan penyakit katastropik yang dapat menimbulkan terjadinya peningkatan beban sosial ekonomi bagi penderita, keluarga maupun pemerintah, karena penanganannya membutuhkan waktu yang tidak sebentar, biaya yang besar dan memerlukan teknologi tinggi. Salah satu contoh PTM adalah penyakit kardiovaskuler. Di Kabupaten Jember, menurut data dari Dinas Kesehatan menyebutkan bahwa terdapat beberapa wilayah puskesmas yang memiliki angka penyakit kardiovaskular yang tinggi seperti Puskesmas Bangsalsari, Kalisat, dan Sumbersari dengan angka lebih dari 100,000 penderita. Kegiatan screening melalui posbindu merupakan salah satu upaya yang dapat dilakukan guna pencegahan dan mengendalikan PTM. Kegiatan pengabdian kepada masyarakat ini bertujuan untuk melakukan screening PTM serta meningkatan keterampilan Resusitasi Jantung Paru (RJP) di masyarakat Desa Kranjingan Kecamatan Sumbersari Kabupaten Jember. Kegiatan pengabdian kepada masyarakat ini dilakukan melalui beberapa tahapan yaitu dimulai dengan penyuluhan, pemeriksaan deteksi dini faktor risiko PTM, konseling serta pelatihan CPR. Kegiatan skrining penting untuk dijalankan untuk dapat meningkatan kesadaran masyarakat terhadap penyakit tidak menular melalui deteksi dini, early treatment serta mengendalikan factor risiko sehingga dapat menurunkan tingkat kesakitan dan kematian. Pasca kegiatan ini diketahui telah terjadi peningkatan pengetahuan peserta terkait dengan keterampilan Resusitasi Jantung Paru (RJP) di masyarakat di Desa Kranjingan Kec. Sumbersari Kab. Jember

Background: Determining the sperm quality can be measured by calculating sperm motility and spermmotility is governed by intracellular calcium (Ca2+) concentration. However, the intracellular calciumincrease found in essential hypertension cases may decrease acrosome reaction and increase ROS production,and may trigger sperm cell apoptosis. Ground cherry (Physalis angulata L) is an herbal plant containingbioflavonoid as antioxidant which is crucial to defend against free radicals and prevent toxic effects.Objective: This study aims to test the capacity of Physalis angulata L. extract, in preventing disruptionof sperm motility in male Wistar strain rats model of hypertension through oxidative stress reduction andcytosolic calcium regulation.Method: Experimental animal male white rat (Rattus novergicus) model of hypertension wereintraperitoneally inducted with L-NAME. The animals were divided into 5 treatment groups and givenPhysalis angulata L. leaf water extract with various doses for 14 consecutive days. At the end of the study,animals were sacrificed, and testes were taken for measuring the MDA levels, intracellular calcium, andsperm motility.Results: Physalis angulata L. leaf water extract is proven to prevent increasing number of testicular MDAlevel, prevent increasing number of sperm intracellular calcium, and prevent decreasing number of motilesperm in male white Wistar strain rat model of hypertension.Conclusion: The results show that the Physalis angulata L. leaf water extract is proven to prevent theincrease of MDA testis level and the increase of intracellular calcium testis amount, and prevent the numberof motile sperm on male Wistar strain white mice model of hypertension.

Phacoemulsification is cataract therapy’s gold standard with lowest complication rate and best visual outcome. However, phacoemulsification is expensive and difficult to use widely. Pathogenesis of cataract related to Crystalline P23T γD protein in congenital cataracts and β-amyloid protein in senile cataract. Papain enzymes and zingibain enzymes are cataract’s alternatives therapy with proteolytic effects that potentially lyse these proteins. Proteolytic injection with 30-gauge needle results in small, safe incision. Papain enzyme from Carica Papaya plant and zingibain enzyme from Zingiber Officinale plant which grows plentifully in Indonesia that becomes cheap source of proteolytic. This study’s purpose is to compare probability of binding energy based on Binding Interaction Model (BIM) between papain and zingibain enzymes against Crystalline P23T γD protein and β-amyloid protein with molecular docking. This research uses https://cluspro.bu.edu./login.php. BIM with lowest binding energy has most stable bond. Docking results show interaction probability between papain enzyme and Crystalline P23T γD protein has lowest binding energy of -730.4 kJ/mol at BIM 1. Probability of interaction between papain enzyme and β-amyloid protein has lowest binding energy of -697.2 kJ/mol at BIM 1. Probability of interaction between the enzyme zingibain and the crystalline P23T protein γD has lowest binding energy of -890.5 kJ/mol at BIM 2. Probability of interaction between the enzyme zingibain and the β-amyloid protein has lowest binding energy of -873.5 kJ/mol at BIM 0. It was concluded that the zingibain enzyme has the most stable probability of forming a stable bond to Crystalline P23T γD protein and β-amyloid protein. Keyword:In silico, papain, zingibain, cataract

Kacang kedelai (Glycine max L. Merr) memiliki kemampuan untuk mencegah terjadinya kerusakanginjal akibat keracunan diazinon karena mengandung tannin, proanthocyanidin, dan isoflavonoid. Tujuanpenelitian ini untuk membuktikan efek pemberian tepung kedelai sebagai nefroprotektor terhadap tikus yangdiinduksi diazinon. Penelitian dilakukan di Laboratorium Fisiologi dan Farmakologi Fakultas KedokteranUniversitas Jember, pada bulan Oktober-Desember 2017. Sebanyak 25 tikus wistar jantan usia 12-15 minggudiacak dan dibagi menjadi 5 kelompok, kelompok kontrol normal (K1) yang diberikan normal salin selama28 hari, kelompok kontrol negatif (K2) yang diberikan normal salin 28 hari dan diazinon selama 5 hari.Kelompok perlakuan yang diberikan larutan tepung kedelai dengan dosis masing-masing (K3) 1 g/10mL,(K4) 1,5 g/10mL, dan (K5) 2 g/10mL selama 28 hari, dilanjutkan diazinon 40 mg/kgBB selama 5 hari. Tikusdisakrifasi menggunakan eter kemudian diambil organ ginjal dan dibuat preparat histopatologi yang diwarnaiHematoksilin-Eosin. Pengamatan berat ringan kerusakan ginjal dinilai berdasarkan skoring yang dikonversimenjadi data interval menggunakan method of successive interval (MSI). Kelompok K1 memiliki rata-rataskor histopatologi terendah yaitu 2,427±0,191, sedangkan rata-rata skor histopatologi tertinggi di kelompokK2 4,272±0,221. Kelompok perlakuan dengan rata-rata skor histopatologi terendah berada pada kelompokK5 yaitu 2,875±0,211. Terdapat perbedaan signifikan terhadap rata-rata skor histopatologi ginjal tikusantarkelompok (Tukey HSD, p Kata kunci: tepung kedelai, diazinon, tikus wistar, kerusakan ginjal. The Effect of Soybean Flour Administration to Renal Histopathology of Diazinon-Induced Wistar Rats Abstract Soybean (Glycine max L. Merr) has the ability to prevent renal injury due to diazinon intoxicationwhich contains tannin, proanthocyanidin, and isoflavonoid. This study was intended to prove the effect ofsoybean flour administration as nephroprotective agent on diazinon induced rats. This study was conductedat Physiology and Pharmacology Laboratory, Faculty of Medicine Universitas Jember on October-December2017. A total of 25 male wistar rats, aged 12 – 15 months were randomized and divided into 5 groups, normalcontrol group (C1) which was given normal saline solution for 28 days, negative control group (C2) which wasgiven diazinon for 5 days and normal saline solution for 28 days. The treated group was given soybean floursolution with 3 different dosages, 1g/10 ml (C3), 1.5 g/10 ml (C4), and 2g/10 ml (C5) for 28 days, continuedwith diazinon administration of 40 mg/kg for 5 days. Rats euthanasia were done using ether, followed bykidney extraction and its histopathology preparation stained by Hematoxylin Eosin. The observation ofrenal injury severity was assessed by a scoring converted into interval data by using method of successiveinternal (MSI). C1 group had the lowest mean histopathology score 2.427±0.191 whereas the highest meanhistopathology score was on group C2 4.272±0.221. The treated group with the lowest mean histopathologyscore was on group C5 2.875±0.211. There was a significant difference of mean kidney histopathologicalscores between groups (Turkey HSD, p Keywords: soybean flour, diazinon, wistar rats, renal injury.