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Phenazine production of the highest, and 50 ppm Cd (Fig. 2 a) in the presence of recombinant bacteria observed that 72 hours (9.18 μg/ml). In the presence of wild bacteria 100 ppm Cd (Fig. 2 b) observed that 72 hours (8.16 μg/ml). In the presence of 50 ppm Cd and 150 ppm Cd 72 hours recombinant bacteria and, In this study, from Vitreoscilla sp. recombinant strains were obtained from cloned bacterial hemoglobin (VHb) gene (vgb) Eh [pUC8:15] and its wild-type strain Erwinia herbicola phenazine production in the presence of heavy metals were investigated. Time-dependent production of phenazine in the wild bacteria not shows a significant change. In generally, at the end of 96 hours in the presence of Pb in terms of production of wild-type bacteria phenazine 2.91 μg/ml of recombinant bacteria is to produce fenazin production 9.5 μg /ml, respectively. The average phenazine highest values were 7, 8.8, 8 and 9.5 μg/ml Cd, Co, Fe and Pb for Eh [pUC8:15]. E. herbicola is 6.32, 3.33, 6.02 and 2.99 μg/ml Cd, Co, Fe and Pb, respectively. Genetic engineering vgb, may be an effective method to increase phenazine production.

Yabancı organizmadaki faydalı etkisi iyi şekilde belirlenmiş olan Vitreoscilla hemoglobinin (VHb) Erwinia herbicola'da L-DOPA ve dopamin üretimi üzerindeki etkisi araştırılmıştır. VHb geni (vgb+) taşıyan rekombinantın sitoplazmik L-DOPA seviyesi (97 mg L-1 ) konakçısından (33.8 mg L-1) ve vgb- kontrol suşundan ( 35.8 mg L-1) oldukça yüksek kaydedilmiştir. Ayrıca, E. herbicola vgb+ rekombinantının dopamin seviyesi konakçısından yaklaşık 100 kat daha fazla bulunmuştur. L-DOPA ve dopaminin en yüksek seviyelerde L- tirozin eklenen zengin kültür ortamının ileri kültür fazda (24 saat) gözlemlenmiştir. Benzer şekilde, tirozin fenol liyaz aktivitesi de en yüksek seviyede aynı kültür fazında belirlenmiştir. Bu enzimin aktivitesinin sitoplazmik L-DOPA ile ilişkili olduğu saptanmıştır. E. herbicola vgb+ suşunun yüksek tirozin fenol liyaz aktivitesi ileri kültür fazında görülmüştür. Given the well-established beneficial effects of Vitreoscilla hemoglobin (VHb) on heterologous organisms, the potential of this protein for the production of L-DOPA and dopamine in Erwinia herbicola, was investigated. The constructed recombinant bearing the VHb gene (vgb+) had substantially higher levels of cytoplasmic L-DOPA ( 97 mg L-1 for E. herbicola) than its respective host (33.8 mg L-1) and the vgb- control strain ( 35.8 mg L-1). Further, the vgb+ recombinants E. herbicola had about two orders of magnitude higher dopamine level than its host. The highest L-DOPA and dopamine levels were observed in post-stationary growth phase (24 h) of cultivation in nutrient rich medium supplemented with L-tyrosine. Similarly, TPL activity was also highest in the same cultures. The activity of tyrosine phenol-lyase, the enzyme converting L-tyrosine to L-DOPA, was well-correlated to cytoplasmic L-DOPA levels. As cultures aged, higher tyrosine phenol-lyase activity of the vgb+ strains was more apparent. 94