7 results on '"Ayaka Doi"'
Search Results
2. Successful Treatment of Post-Phacoemulsification Descemet’s Membrane Detachment Assessed by Anterior Segment Optical Coherence Tomography: A Case Report
- Author
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Ayaka Doi, Tadamichi Akagi, and Akitaka Tsujikawa
- Subjects
descemet’s membrane detachment ,cataract surgery ,gas tamponade ,anterior segment optical coherence tomography ,Ophthalmology ,RE1-994 - Abstract
Descemet’s membrane detachment (DMD) is a rare but serious complication of phacoemulsification surgery. A small DMD may resolve spontaneously, but extensive DMD often requires intracameral injection of air, nonexpansile gases, or expansile gases. A 92-year-old man who underwent phacoemulsification and aspiration with intraocular lens placement in the right eye had significantly reduced visual acuity, with a hazy cornea after surgery. Anterior segment optical coherence tomography (AS-OCT) examination revealed extensive DMD throughout the cornea. He was treated with intracameral injection of 20% sulfur hexafluoride. As a result, the Descemet membrane was successfully reattached, and the corneal edema resolved. AS-OCT was helpful in confirming the presence and extent of DMD, provided useful information to determine the appropriate treatment, and was useful for monitoring DMD.
- Published
- 2021
- Full Text
- View/download PDF
3. Long-Term Visual Outcome in Inferior Posterior Staphyloma and Efficacy of Treatment for Complicated Choroidal Neovascularization
- Author
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Ayako Takahashi, Sotaro Ooto, Hiroshi Tamura, Masahiro Miyake, Akihito Uji, Naoko Ueda-Arakawa, Manabu Miyata, Tomotaka Wakazono, Akitaka Tsujikawa, Kenji Yamashiro, Yuki Muraoka, and Ayaka Doi
- Subjects
medicine.medical_specialty ,Visual acuity ,genetic structures ,Clinical cohort ,Visual Acuity ,Angiogenesis Inhibitors ,Cohort Studies ,03 medical and health sciences ,0302 clinical medicine ,Ranibizumab ,Ophthalmology ,Humans ,Medicine ,Treatment effect ,Prospective Studies ,Fluorescein Angiography ,030304 developmental biology ,0303 health sciences ,business.industry ,Choroidal Neovascularization ,eye diseases ,Treatment Outcome ,Choroidal neovascularization ,Intravitreal Injections ,030221 ophthalmology & optometry ,Posterior staphyloma ,sense organs ,medicine.symptom ,business ,Retinal exudate - Abstract
Purpose To investigate long-term visual outcome in inferior posterior staphyloma (IPS) in each group classified based on macular complications and to examine the treatment effect for eyes with IPS with choroidal neovascularization (CNV). Design Prospective clinical cohort study. Participants We analyzed 56 eyes of 43 consecutive patients with IPS who were followed for 4 years. Methods We classified eligible eyes into 3 groups based on baseline findings: eyes without CNV or retinal exudate (no-exudate group), eyes without CNV and with retinal exudate (exudate group), and eyes with CNV (CNV group). We investigated the best-corrected visual acuity (BCVA) and associated parameters for 4 years. Results BCVA declined during 4 years only in the exudate group (P = .002), whereas it was maintained for 4 years in the no-exudate and CNV groups (P = .53 and .20, respectively). Baseline BCVA was lower in the CNV group than in the exudate group (P = .004); however, the 4-year BCVA was not (P = .84). The 4-year BCVA was associated with baseline BCVA in all groups. Eyes in the CNV group required 9.0 ± 8.7 anti–vascular endothelial growth factor therapy in 4 years. Conclusions Better baseline BCVA in eyes with exudative IPS without CNV spontaneously declined in 4 years, whereas worse baseline BCVA in eyes with IPS with CNV did not, probably because of treatment for retinal exudate from CNV. Anti–vascular endothelial growth factor therapy would be effective for long-term maintenance of BCVA in eyes with IPS with CNV, similar to other diseases with CNV.
- Published
- 2021
4. Successful Treatment of Post-Phacoemulsification Descemet’s Membrane Detachment Assessed by Anterior Segment Optical Coherence Tomography: A Case Report
- Author
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Akitaka Tsujikawa, Tadamichi Akagi, and Ayaka Doi
- Subjects
musculoskeletal diseases ,medicine.medical_specialty ,congenital, hereditary, and neonatal diseases and abnormalities ,genetic structures ,medicine.medical_treatment ,descemet’s membrane detachment ,Case Report ,Intraocular lens ,Optical coherence tomography ,Ophthalmology ,Cornea ,medicine ,gas tamponade ,anterior segment optical coherence tomography ,Reduced visual acuity ,medicine.diagnostic_test ,business.industry ,Phacoemulsification ,cataract surgery ,Cataract surgery ,RE1-994 ,eye diseases ,Descemet's membrane ,medicine.anatomical_structure ,Descemet's membrane detachment ,sense organs ,business ,Complication - Abstract
Descemet’s membrane detachment (DMD) is a rare but serious complication of phacoemulsification surgery. A small DMD may resolve spontaneously, but extensive DMD often requires intracameral injection of air, nonexpansile gases, or expansile gases. A 92-year-old man who underwent phacoemulsification and aspiration with intraocular lens placement in the right eye had significantly reduced visual acuity, with a hazy cornea after surgery. Anterior segment optical coherence tomography (AS-OCT) examination revealed extensive DMD throughout the cornea. He was treated with intracameral injection of 20% sulfur hexafluoride. As a result, the Descemet membrane was successfully reattached, and the corneal edema resolved. AS-OCT was helpful in confirming the presence and extent of DMD, provided useful information to determine the appropriate treatment, and was useful for monitoring DMD.
- Published
- 2021
5. Reconstitution of an Initial Step of Phototropin Signaling in Stomatal Guard Cells
- Author
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Ayaka Doi, Koji Okajima, Ken-ichiro Shimazaki, Sayumi Yoshida, Atsushi Takemiya, and Satoru Tokutomi
- Subjects
0301 basic medicine ,Phototropins ,Light Signal Transduction ,animal structures ,Phototropin ,Light ,Physiology ,Phosphatase ,Arabidopsis ,Carbazoles ,Plant Science ,Protein Serine-Threonine Kinases ,Biology ,Indole Alkaloids ,03 medical and health sciences ,Substrate-level phosphorylation ,Guard cell ,Phosphorylation ,Photosynthesis ,Phototropism ,Protein kinase A ,Arabidopsis Proteins ,Kinase ,Phosphotransferases ,Cell Biology ,General Medicine ,Darkness ,Phosphoproteins ,Recombinant Proteins ,Cell biology ,030104 developmental biology ,Biochemistry ,Protein kinase domain ,Plant Stomata ,Mutagenesis, Site-Directed - Abstract
Phototropins are light-activated receptor kinases that mediate a wide range of blue light responses responsible for the optimization of photosynthesis. Despite the physiological importance of phototropins, it is still unclear how they transduce light signals into physiological responses. Here, we succeeded in reproducing a primary step of phototropin signaling in vitro using a physiological substrate of phototropin, the BLUS1 (BLUE LIGHT SIGNALING1) kinase of guard cells. When PHOT1 and BLUS1 were expressed in Escherichia coli and the resulting recombinant proteins were incubated with ATP, white and blue light induced phosphorylation of BLUS1 but red light and darkness did not. Site-directed mutagenesis of PHOT1 and BLUS1 revealed that the phosphorylation was catalyzed by phot1 kinase. Similar to stomatal blue light responses, the BLUS1 phosphorylation depended on the fluence rate of blue light and was inhibited by protein kinase inhibitors, K-252a and staurosporine. In contrast to the result in vivo, BLUS1 was not dephosphorylated in vitro, suggesting the involvement of a protein phosphatase in the response in vivo. phot1 with a C-terminal kinase domain but devoid of the N-terminal domain, constitutively phosphorylated BLUS1 without blue light, indicating that the N-terminal domain has an autoinhibitory action and prevents substrate phosphorylation. The results provide the first reconstitution of a primary step of phototropin signaling and a clue for understanding the molecular nature of this process.
- Published
- 2015
6. Effects of Gosha-jinki-gan (Chinese herbal medicine: Niu-Che-SenQi-Wan) on hyperinsulinemia induced in rats fed a sucrose-rich diet
- Author
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Ryuji Kato, Yoshio Ijiri, Yoshihiko Hirotani, Ayaka Doi, Kazuhiko Tanaka, Kenji Ikeda, and Michiaki Myotoku
- Subjects
medicine.medical_specialty ,Plasma glucose ,Sucrose ,business.industry ,Insulin ,medicine.medical_treatment ,Dietary Sucrose ,General Medicine ,medicine.disease ,chemistry.chemical_compound ,Endocrinology ,chemistry ,Internal medicine ,medicine ,Hyperinsulinemia ,Pharmacology (medical) ,General Pharmacology, Toxicology and Pharmaceutics ,Plasma insulin ,business - Abstract
We investigated the effects of a Chinese herbal medicine, Gosha-jinki-gan (GJG), on the regulation of insulin levels in rats fed a sucrose-rich diet (SRD). Normal Wistar rats in the SRD group were fed an SRD for 4 weeks. Increased dietary sucrose did not alter plasma glucose levels but it increased plasma insulin levels at 2 and 4 weeks in the SRD-fed rats relative to control rats that were fed standard chow. GJG treatment significantly suppressed the SRD-induced elevation in plasma insulin levels. These results suggest that GJG improves hyperinsulinemia caused by an SRD.
- Published
- 2011
7. Reconstitution of an Initial Step of Phototropin Signaling in Stomatal Guard Cells.
- Author
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Atsushi Takemiya, Ayaka Doi, Sayumi Yoshida, Koji Okajima, Satoru Tokutomi, and Ken-ichiro Shimazaki
- Subjects
- *
PHOTOTROPINS , *STOMATA , *GUARD cells (Plant anatomy) , *CELLULAR signal transduction , *PROTEIN kinase inhibitors - Abstract
Phototropins are light-activated receptor kinases that mediate a wide range of blue light responses responsible for the optimization of photosynthesis. Despite the physiological importance of phototropins, it is still unclear how they transduce light signals into physiological responses. Here, we succeeded in reproducing a primary step of phototropin signaling in vitro using a physiological substrate of phototropin, the BLUS1 (BLUE LIGHT SIGNALING1) kinase of guard cells. When PHOT1 and BLUS1 were expressed in Escherichia coli and the resulting recombinant proteins were incubated with ATP, white and blue light induced phosphorylation of BLUS1 but red light and darkness did not. Site-directed mutagenesis of PHOT1 and BLUS1 revealed that the phosphorylation was catalyzed by phot1 kinase. Similar to stomatal blue light responses, the BLUS1 phosphorylation depended on the fluence rate of blue light and was inhibited by protein kinase inhibitors, K-252a and staurosporine. In contrast to the result in vivo, BLUS1 was not dephosphorylated in vitro, suggesting the involvement of a protein phosphatase in the response in vivo. phot1 with a Cterminal kinase domain but devoid of the N-terminal domain, constitutively phosphorylated BLUS1 without blue light, indicating that the N-terminal domain has an autoinhibitory action and prevents substrate phosphorylation. The results provide the first reconstitution of a primary step of phototropin signaling and a clue for understanding the molecular nature of this process. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
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