1. AXIN2 Reduces the Survival of Porcine Induced Pluripotent Stem Cells (piPSCs).
- Author
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Zhang R, Yu S, Shen Q, Zhao W, Zhang J, Wu X, Zhu Z, Wu X, Li N, Peng S, and Hua J
- Subjects
- Animals, Apoptosis genetics, Apoptosis physiology, Axin Protein deficiency, Axin Protein genetics, Cell Differentiation genetics, Cell Differentiation physiology, Cell Proliferation drug effects, Cell Proliferation genetics, Cell Proliferation physiology, Cell Survival genetics, Cell Survival physiology, Cells, Cultured, Cyclin D1 genetics, Cyclin D1 metabolism, Gene Knockdown Techniques, Induced Pluripotent Stem Cells cytology, Induced Pluripotent Stem Cells drug effects, Models, Biological, Pyridines pharmacology, Pyrimidines pharmacology, RNA, Messenger genetics, RNA, Messenger metabolism, RNA-Seq, Swine, Up-Regulation, Wnt Signaling Pathway drug effects, Wnt Signaling Pathway genetics, Wnt Signaling Pathway physiology, beta Catenin genetics, beta Catenin metabolism, Axin Protein physiology, Induced Pluripotent Stem Cells physiology
- Abstract
The establishment of porcine pluripotent stem cells (piPSCs) is critical but remains challenging. All piPSCs are extremely sensitive to minor perturbations of culture conditions and signaling network. Inhibitors, such as CHIR99021 and XAV939 targeting the WNT signaling pathway, have been added in a culture medium to modify the cell regulatory network. However, potential side effects of inhibitors could confine the pluripotency and practicability of piPSCs. This study aimed to investigate the roles of AXIN, one component of the WNT pathway in piPSCs. Here, porcine AXIN1 and AXIN2 genes were knocked-down or overexpressed. Digital RNA-seq was performed to explore the mechanism of cell proliferation and apoptosis. We found that (1) overexpression of the porcine AXIN2 gene significantly reduced survival and negatively impacted the pluripotency of piPSCs, and (2) knockdown of AXIN2, a negative effector of the WNT signaling pathway, enhanced the expression of genes involved in cell cycle but reduced the expression of genes related to cell differentiation, death, and apoptosis.
- Published
- 2021
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