107 results on '"Avti P"'
Search Results
2. A prospective case–control study on the evaluation of oxidative stress in renal stone formers
- Author
-
Manoharan, V., Sharma, G., Devana, S. K., Sharma, S., Avti, P., and Chandramouli, S.
- Published
- 2024
- Full Text
- View/download PDF
3. Luminescent single-walled carbon nanotube-sensitized europium nanoprobes for cellular imaging
- Author
-
Avti PK and Sitharaman B
- Subjects
Medicine (General) ,R5-920 - Abstract
Pramod K Avti, Balaji SitharamanDepartment of Biomedical Engineering, Stony Brook University, Stony Brook, NY, USAAbstract: Lanthanoid-based optical probes with excitation wavelengths in the ultra-violet (UV) range (300–325 nm) have been widely developed as imaging probes. Efficient cellular imaging requires that lanthanoid optical probes be excited at visible wavelengths, to avoid UV damage to cells. The efficacy of europium-catalyzed single-walled carbon nanotubes (Eu-SWCNTs), as visible nanoprobes for cellular imaging, is reported in this study. Confocal fluorescence microscopy images of breast cancer cells (SK-BR-3 and MCF-7) and normal cells (NIH 3T3), treated with Eu-SWCNT at 0.2 µg/mL concentration, showed bright red luminescence after excitation at 365 nm and 458 nm wavelengths. Cell viability analysis showed no cytotoxic effects after the incubation of cells with Eu-SWCNTs at this concentration. Eu-SWCNT uptake is via the endocytosis mechanism. Labeling efficiency, defined as the percentage of incubated cells that uptake Eu-SWCNT, was 95%–100% for all cell types. The average cellular uptake concentration was 6.68 ng Eu per cell. Intracellular localization was further corroborated by transmission electron microscopy and Raman microscopy. The results indicate that Eu-SWCNT shows potential as a novel cellular imaging probe, wherein SWCNT sensitizes Eu3+ ions to allow excitation at visible wavelengths, and stable time-resolved red emission. The ability to functionalize biomolecules on the exterior surface of Eu-SWCNT makes it an excellent candidate for targeted cellular imaging.Keywords: cellular imaging, confocal microscopy, europium, single-walled carbon nanotubes, luminescence, visible excitation
- Published
- 2012
4. An Atomic Level Investigation of Sodium Ions Regulating Agonist and Antagonist Binding in the Active Site of a Novel Target 5HT2BR Against Drug-Resistant Epilepsy
- Author
-
Chauhan, Arushi, Singh, Jitender, Sangwan, Namrata, Dhawan, Radhika, and Avti, Pramod K.
- Published
- 2023
- Full Text
- View/download PDF
5. Unravelling benzazepines and aminopyrimidine as multi-target therapeutic repurposing drugs for EGFR V774M mutation in neuroglioma patients
- Author
-
Jitender Singh, Krishan L Khanduja, and Pramod K Avti
- Subjects
allele frequencies ,egfr mutations ,gibbs free energy ,glioma ,missense-mutation ,multi-target ,Medicine (General) ,R5-920 ,Biology (General) ,QH301-705.5 - Abstract
Introduction: Neuroglioma, a classification encompassing tumors arising from glial cells, exhibits variable aggressiveness and depends on tumor grade and stage. Unraveling the EGFR gene alterations, including amplifications (unaltered), deletions, and missense mutations (altered), is emerging in glioma. However, the precise understanding of emerging EGFR mutations and their role in neuroglioma remains limited. This study aims to identify specific EGFR mutations prevalent in neuroglioma patients and investigate their potential as therapeutic targets using FDA- approved drugs for repurposing approach. Methods: Neuroglioma patient’s data were analyzed to identify the various mutations and survival rates. High throughput virtual screening (HTVS) of FDA-approved (1615) drugs using molecular docking and simulation was executed to determine the potential hits. Results: Neuroglioma patient samples (n=4251) analysis reveals 19% EGFR alterations with most missense mutations at V774M in exon 19. The Kaplan-Meier plots show that the overall survival rate was higher in the unaltered group than in the altered group. Docking studies resulted the best hits based on each target's higher docking score, minimum free energy (MMGBSA), minimum kd, ki, and IC50 values. MD simulations and their trajectories show that compounds ZINC000011679756 target unaltered EGFR and ZINC000003978005 targets altered EGFR, whereas ZINC000012503187 (Conivaptan, Benzazepine) and ZINC000068153186 (Dabrafenib, aminopyrimidine) target both the EGFRs. The shortlisted compounds demonstrate favorable residual interactions with their respective targets, forming highly stable complexes. Moreover, these shortlisted compounds have drug- like properties as assessed by ADMET profiling. Conclusion: Therefore, compounds (ZINC000012503187 and ZINC000068153186) can effectively target both the unaltered/altered EGFRs as multi-target therapeutic repurposing drugs towards neuroglioma.
- Published
- 2024
- Full Text
- View/download PDF
6. Yogic Neti-Kriya Using Povidone Iodine: Can it have a Preventive Role Against SARS-CoV-2 Infection Gateway?
- Author
-
Sarma, Phulen, Bhattacharyya, Anusuya, Prakash, Ajay, Kaur, Hardeep, Prajapat, Manisha, Borah, Mukundam, Kumar, Subodh, Bansal, Seema, Sharma, Saurabh, Kaur, Gurjeet, Kumar, Harish, Sharma, Dibya Jyoti, Das, Karuna Kumar, Avti, Pramod, and Medhi, Bikash
- Published
- 2022
- Full Text
- View/download PDF
7. Structural-Based Virtual Screening of FDA-Approved Drugs Repository for NSP16 Inhibitors, Essential for SARS-COV-2 Invasion Into Host Cells: Elucidation From MM/PBSA Calculation
- Author
-
Subodh Kumar, Harvinder Singh, Manisha Prajapat, Phulen Sarma, Anusuya Bhattacharyya, Hardeep Kaur, Gurjeet Kaur, Nishant Shekhar, Karanveer Kaushal, Kalpna Kumari, Seema Bansal, Saniya Mahendiratta, Arushi Chauhan, Ashutosh Singh, Rahul Soloman Singh, Saurabh Sharma, Prasad Thota, Pramod Avti, Ajay Prakash, Anurag Kuhad, and Bikash Medhi
- Subjects
Biology (General) ,QH301-705.5 - Abstract
NSP16 is one of the structural proteins of the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) necessary for its entrance to the host cells. It exhibits 2’O-methyl-transferase (2’O-MTase) activity of NSP16 using methyl group from S-adenosyl methionine (SAM) by methylating the 5-end of virally encoded mRNAs and shields viral RNA, and also controls its replication as well as infection. In the present study, we used in silico approaches of drug repurposing to target and inhibit the SAM binding site in NSP16 using Food and Drug Administration (FDA)-approved small molecules set from Drug Bank database. Among the 2 456 FDA-approved molecules, framycetin, paromomycin, and amikacin were found to be significant binders against the SAM binding cryptic pocket of NSP16 with docking score of –13.708, –14.997 and –15.841 kcal/mol, respectively. Classical molecular dynamics (MD) simulation and molecular mechanics Poisson−Boltzmann surface area (MM/PBSA)-based binding free energy calculation depicted that all these three framycetin, paromomycin, and amikacin might be promising therapeutic leads towards SARS-CoV-2 infections via host immune escape inhibition pathway.
- Published
- 2023
- Full Text
- View/download PDF
8. Screening and identification of phytochemical drug molecules against mutant BRCA1 receptor of breast cancer using computational approaches
- Author
-
Singh, Jitender, Sangwan, Namrata, Chauhan, Arushi, Sarma, Phulen, Prakash, Ajay, Medhi, Bikash, and Avti, Pramod K.
- Published
- 2022
- Full Text
- View/download PDF
9. Effect of breathing intervention in patients with COVID and healthcare workers
- Author
-
Manjari Rain, Goverdhan Dutt Puri, Aashish Bhalla, Pramod Avti, Balachundhar Subramaniam, Vipin Kaushal, Vinod Srivastava, Pranay Mahajan, Mini Singh, Navin Pandey, Pankaj Malhotra, Sonu Goel, Krishan Kumar, Naresh Sachdeva, Kalyan Maity, Prashant Verma, Nishant Dixit, Sheetal Jindal Gupta, Priya Mehra, Pooja Nadholta, Radhika Khosla, Shweta Ahuja, and Akshay Anand
- Subjects
COVID-19 ,breathing technique ,D-dimer ,mindfulness ,yoga ,SpO2 ,Public aspects of medicine ,RA1-1270 - Abstract
BackgroundRegulated breathing facilitates ventilation and reduces breathlessness. However, the effect of Yogic breathing on patients with COVID remains unclear. We aimed to evaluate the efficacy of two breathing protocols, i.e., short breathing technique (SBT) and long duration breathing technique (LBDT).MethodsThree groups including COVID-positive patients, COVID-recovered patients, and healthcare workers (HCWs) were included in the study and segregated into Yoga and control groups. SBT was administered to COVID-positive patients. Both SBT and LBDT were administered to COVID-recovered patients and HCWs. A total of 18 biochemical parameters, a 6-min walk test (6MWT), and a 1-min sit-stand test (1MSST) were assessed on 0th, 7th, and 15th days, where biochemical parameters were the primary outcome. Pre-post estimation of neuropsychological parameters (nine questionnaires) and heart rate variability (HRV) were carried out. The paired t-test or Wilcoxon rank test was applied for pre-post comparison and the Student's t-test or Mann–Whitney U test was used for group comparison. Repeated measures test was applied for data recorded at three time points.ResultsA significant elevation in white blood cell (WBC) count was observed in COVID-positive intervention (p < 0.001) and control groups (p = 0.003), indicating no role of intervention on change in WBC number. WBC count (p = 0.002) and D-dimer (p = 0.002) significantly decreased in the COVID-recovered intervention group. D-dimer was also reduced in HCWs practicing Yogic breathing as compared to controls (p = 0.01). D-dimer was the primary outcome, which remained below 0.50 μg/ml (a cutoff value to define severity) in the COVID-positive yoga group (CYG) and decreased in the COVID-recovered yoga group (RYG) and the HCW yoga group (HYG) after intervention. A 6-min walk test (6MWT) showed an increase in distance covered among the COVID-positive patients (p = 0.01) and HCWs (p = 0.002) after intervention. The high-frequency power (p = 0.01) was found to be reduced in the COVID-positive intervention group. No significant change in neuropsychological parameters was observed.ConclusionYogic breathing lowered D-dimer, which is helpful in reducing thrombosis and venous thromboembolism in patients with COVID-19 besides lowering the chances of vaccine-induced thrombotic thrombocytopenia in vaccinated individuals. The breathing intervention improved exercise capacity in mild to moderate cases of COVID-19. Further studies can show if such breathing techniques can influence immunity-related genes, as reported recently in a study. We suggest that Yogic breathing may be considered an integrative approach for the management of patients with COVID.Trial registrationhttp://ctri.nic.in/Clinicaltrials/login.php, identifier: CTRI/2020/10/028195.
- Published
- 2022
- Full Text
- View/download PDF
10. Bimetallic nanoparticles green synthesis from litchi leaf extract: a promising approach for breast cancer treatment
- Author
-
Ramanjeet Kaur, Jitender Singh, Pramod K Avti, Vivek Kumar, and Rajesh Kumar
- Subjects
bimetallic ,breast cancer ,green synthesis ,GCNPs ,SCNPs ,Zeta potential ,Chemical technology ,TP1-1185 - Abstract
Noble metal nanoparticles have demonstrated promising biomedical and nanomedicine applications, and their bimetallic equivalents from the green synthesis approach are expected to be more promising. This study concerns bimetallic nanoparticle’s synthesis, characterization, and structure-function analysis for their potential application in breast cancer. Silver core (SCNPs) and Gold core (GCNPs) bimetallic nanoparticles were synthesized using Litchi Chinesis leaf extract (LCLE) and characterized using various physio-chemical techniques. The results revealed the successful synthesis of SCNPs and GCNPs with distinct surface plasmon resonance peaks at 551 nm and 531 nm, hydrodynamic sizes of 66 nm and 53 nm, and Zeta potential values of −26.0 mV and −20.6 mV. XRD analysis confirmed the presence of silver and gold phases, while HR-TEM images revealed spherical shapes for SCNPs and heterogenous shapes for GCNPs. Both nanoparticles demonstrated dose and time-dependent inhibition of breast cancer cell growth, with GCNPs requiring a higher concentration than SCNPs at 48 h compared to 24 h. Cell cycle evaluation indicated a cell cycle arrest in the G2M phase for both nanoparticles, an impact on the S phase distribution, and reactive oxygen species (ROS) generation, further contributing to their antiproliferative effects.
- Published
- 2023
- Full Text
- View/download PDF
11. Surfactant Mediated Synthesis of Noble Metal Nanoparticles and Their Cytotoxic Effects on Breast Cancer Cells.
- Author
-
KAUR, RAVNEET, SAINI, A., SINGH, J., AVTI, P. K., KUMAR, VIVEK, and KUMAR, RAJESH
- Subjects
METAL nanoparticles ,PRECIOUS metals ,CANCER cells ,BREAST cancer ,GOLD nanoparticles ,NANOMEDICINE - Abstract
The biomedical applications of the noble metal nanoparticles have been the field of interest, especially in cancer therapy. In this paper, the synthesis of spherical silver and gold nanoparticles and their apoptotic activity against breast cancer cells is reported. The chemicals reduction method is used to synthesize nanoparticles. Two different types of surfactants i.e., citrate and polyvinylpyrrolidone were used as a reducing and capping agent. The synthesized nanoparticles have been characterized using X-ray diffraction, ultraviolet-visible absorption spectroscopy, dynamic light scattering, zeta potential and transmission electron microscopy. The synthesized nanoparticles have crystalline phase with average size in the range of 25-30 nm and possess negative surface charge. In vitro studies of nanoparticles against breast cancer cells (MCF-7), were performed. The results show the significant cytotoxicity of nanoparticles against the cancer cell line and found to be mediated by DNA damage. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
12. Adsorptive Removal of Iron Using SiO2 Nanoparticles Extracted from Rice Husk Ash
- Author
-
Tan Tai Nguyen, Hoa Thai Ma, Pramod Avti, Mohammed J. K. Bashir, Choon Aun Ng, Ling Yong Wong, Hieng Kiat Jun, Quang Minh Ngo, and Ngoc Quyen Tran
- Subjects
Analytical chemistry ,QD71-142 - Abstract
In this work, SiO2 nanoparticles were prepared by the sol-gel method after sodium silicate was extracted from rice husk ash (RHA) under various experimental conditions such as types of acids, NaOH concentration, dissolved time, and temperature and used for removal of Fe2+ ions from aqueous solutions. The extracted SiO2 was morphologically and chemically characterized and showed a surface area of 78 m2/g and uniform pores of 2.71 nm, offering high adsorption capacity for Fe2+ ions. The influence of pH, contact time, and amount of adsorbent was studied in order to establish the best conditions for the Fe2+ adsorption and removal. Furthermore, the adsorption data were fitted with an exponential shape curve for all the three variable parameters that affect the adsorption process. The best results were obtained for pH 5, 20 min contact time, and 0.5 g adsorbent dose. The loading adsorption capacity was 9 mg of Fe2+ ions/g SiO2 in the concentration range 0.1–1.0 mgL−1. In addition, the synthesized SiO2 with the size of around 50 nm can be used for specific heavy metal removal and drug delivery, after modification of the SiO2 surface with various functional groups.
- Published
- 2019
- Full Text
- View/download PDF
13. Allosteric modulation of conserved motifs and helices in 5HT2BR: Advances drug discovery and therapeutic approach towards drug resistant epilepsy
- Author
-
Chauhan, Arushi, Sangwan, Namrata, Singh, Jitender, Prakash, Ajay, Medhi, Bikash, and Avti, Pramod K.
- Abstract
AbstractThe 5HT2BR, class-A GPCR is a new target, and its significance for seizure reduction in Dravet syndrome is just now gaining interest, suggesting its specific role in epileptic seizure management. Homology modeling of human 5HT2BR (P41595), was performed using a template 4IB4, the modeled structure was cross-validated (stereo chemical hindrance, Ramachandran plot, enrichment analysis) to mimic a closer native structure. Virtual screening (8532 compounds), drug-likeliness, mutagenicity, and carcinogenicity profiling prioritized six compounds for molecular dynamics (500 ns), Rgyr,DCCM. The receptor’s C-alpha fluctuation upon bound agonist (6.91 Å), known antagonist (7.03 Å), and LAS 52115629 (5.83 Å) binding varies, leading to receptor stabilization. The residues C-alpha side-chain in active site strongly interacts (hydrogen bonds) with bound agonist (100% interaction: ASP135), known antagonist (95%:ASP135), and LAS 52115629 (100%:ASP135). The Rgyrfor receptor-ligand complex, LAS 52115629 (25.68 Å), lies close to bound agonist-Ergotamine, and DCCM analysis also shows strong positive correlations for LAS 52115629 as compared to known drugs. LAS 52115629 is less likely to cause toxicity than known drugs. The structural parameters in the modeled receptor’s conserved motifs (DRY, PIF, NPY) were altered for receptor activation upon ligand-binding, which otherwise was in the in-activated state. The ligand (LAS 52115629)-binding further alters the helices-III, V, VI (G-protein bound), and VII, which form potential interacting sites with the receptor and are proven necessary for activating the receptor. Therefore, LAS 52115629 can act as a potential 5HT2BR agonist, targeting drug-resistant epilepsy.Communicated by Ramaswamy H. Sarma
- Published
- 2023
- Full Text
- View/download PDF
14. Structure and dynamic simulation-based interactions of benzenoids, pyrroles and organooxygen compounds for effective targeting of GPX4 in ischemic stroke
- Author
-
Sangwan, Namrata, Singh, Jitender, Chauhan, Arushi, Prakash, Ajay, Khanduja, Krishan L., Medhi, Bikash, and Avti, Pramod K
- Abstract
AbstractThe discovery of a novel drug for ischemic stroke is plagued by expensive and unsuccessful outcomes. FDA-approved drugs could be a viable repurposing strategy for stroke therapy. Emerging evidence suggests the regulating role of Glutathione peroxidase (GPX4) in stroke and attracts as a potential target. To overcome limited therapeutic interventions, a drug repurposing in silicoinvestigation of FDA-approved drugs is proposed for the GPX4 receptor in distinctive species (Homo sapiensand Mus musculus). The GPX4 UniProt wild type ids, that is, P36969 (Homo sapiens), P36970 (Rattus norvegicus) and O70325 (Mus musculus) are Swiss modelled, and resultant templates are 2OBI and 6HN3 for Homo sapiens, and 5L71 for Mus musculuswith a sequence identity of ∼88%. Enrichment analysis reveals high sensitivity and ranked actives with ROC and AUC values of 0.59 and 0.61, respectively. Virtual screening at extra precision resulted hit Acarbosum, is similar between 2OBI and 6HN3, demonstrating a multiple-target specificity and Iopromide, targeting 2OBI. MD simulation at 100 ns following trajectory analysis provides RMSD (∼1.2–1.8Å), RMSF (∼1.6–2.7Å), Rgyr (∼15–15.6Å) depicting stabilisation of receptor–ligand complexes. Furthermore, average B-factor value of 2OBI, 6HN3 and 5L71 is 25Å, 24Å and 60Å with a defined resolution of 1.55Å, 1.01Å and 1.80Å, respectively, depicting the thermodynamic stability of the protein structures. The dynamic cross-correlation and principal component analysis of residual fluctuations reveal more positive correlation, high atomic displacements and greater residual clustering of residues from atomic coordinates. Therefore, Acarbosum, an FDA-approved drug, could act as a potential repurposing drug with a multi-target approach translating from preclinical to clinical stages.Communicated by Ramaswamy H. Sarma
- Published
- 2023
- Full Text
- View/download PDF
15. Compromised microvascular oxygen delivery increases brain tissue vulnerability with age
- Author
-
Moeini, Mohammad, Lu, Xuecong, Avti, Pramod K., Damseh, Rafat, Bélanger, Samuel, Picard, Frédéric, Boas, David, Kakkar, Ashok, and Lesage, Frédéric
- Published
- 2018
- Full Text
- View/download PDF
16. Terpenoid analogues as putative therapeutic agents towards glutathione peroxidase (GPX4) in neurodegenerative disorders: a dynamic computational approach
- Author
-
Sangwan, Namrata, Singh, Jitender, Chauhan, Arushi, Prakash, Ajay, Khanduja, Krishan L., Medhi, Bikash, and Avti, Pramod K.
- Abstract
AbstractCarvacrol, a monoterpenoid phenolic phytochemical, a potent antioxidant, and neuroprotective agent is an emerging neuroprotective agent for neurodegenerative diseases (NDDs). Considering scarce information on carvacrol analogues, we hypothesized an in silicoinvestigation emphasizing their preferential binding towards glutathione peroxidase (GPX4) as a target across different species for evaluating through preclinical to clinical studies (2OBI and 6HN3 for Homo sapiens; 5L71 for Mus musculus). Enrichment analysis suggests that ROC (0.59) and AUC (0.61) values have higher sensitivity and significant number of ranked actives. Extra Precision (XP) of 59 compounds was conducted, followed by molecular dynamics and trajectory analysis. Top three hits were chosen for each target i.e., 101203408, 101419546, 59294 (2OBI); 101419546, 100938426, and 28092 (6HN3); and 12059, 52434, 335 (5L71) implying high docking score. 101419546 is common among 2OBI and 6HN3 targets, indicating a multi-target approach. Trajectory analysis of hits provides a permissible range of RMSD, RMSF, Rgyr (∼1.3–2 Å, ∼0.84–1.09 Å, ∼15.05–15.29 Å). Overlapped dynamically simulated 3D-structures of Apo and complexes display significant conformational changes in RMSD of the complexes (∼1.40–2.0 Å) in contrast to Apo (∼1.3–1.8 Å), suggesting structural stability and compactness of the complexes within 45–90 ns. DCCM and PCA analysis shows positive correlation and residual clustering among residues of complexes. The establishment of firm H-bonding, favorable aromaticity and ADMET profile makes them promising drugs across various GPX4 targets among the species. Studies considering the targets across different species aids in anticipating and discovering a common compound for future NDDs therapeutics from bench to bedside.Communicated by Ramaswamy H. Sarma
- Published
- 2023
- Full Text
- View/download PDF
17. Alkyne-Azide 'Click' Chemistry in Designing Nanocarriers for Applications in Biology
- Author
-
Pramod K. Avti, Dusica Maysinger, and Ashok Kakkar
- Subjects
click chemistry ,copper catalyzed alkyne-azide cycloaddition ,drug delivery ,lipid bodies ,mitochondria ,Organic chemistry ,QD241-441 - Abstract
The alkyne-azide cycloaddition, popularly known as the “click” reaction, has been extensively exploited in molecule/macromolecule build-up, and has offered tremendous potential in the design of nanomaterials for applications in a diverse range of disciplines, including biology. Some advantageous characteristics of this coupling include high efficiency, and adaptability to the environment in which the desired covalent linking of the alkyne and azide terminated moieties needs to be carried out. The efficient delivery of active pharmaceutical agents to specific organelles, employing nanocarriers developed through the use of “click” chemistry, constitutes a continuing topical area of research. In this review, we highlight important contributions click chemistry has made in the design of macromolecule-based nanomaterials for therapeutic intervention in mitochondria and lipid droplets.
- Published
- 2013
- Full Text
- View/download PDF
18. Dendrimers as anti-inflammatory agents
- Author
-
Pramod K. Avti and Ashok Kakkar
- Subjects
Dendrímeros ,Nanotransportadores ,Agentes antiinflamatórios ,Ciclooxigenase (COX-2) ,Óxido nítrico sintase induzível (iNOS) ,Citocinas pró-inflamatórias ,Pharmacy and materia medica ,RS1-441 - Abstract
Dendrimers constitute an intriguing class of macromolecules which find applications in a variety of areas including biology. These hyperbranched macromolecules with tailored backbone and surface groups have been extensively investigated as nanocarriers for gene and drug delivery, by molecular encapsulation or covalent conjugation. Dendrimers have provided an excellent platform to develop multivalent and multifunctional nanoconjugates incorporating a variety of functional groups including drugs which are known to be anti-inflammatory agents. Recently, dendrimers have been shown to possess anti-inflammatory properties themselves. This unexpected and intriguing discovery has provided an additional impetus in designing novel active pharmaceutical agents. In this review, we highlight some of the recent developments in the field of dendrimers as nanoscale anti-inflammatory agents.
- Published
- 2013
- Full Text
- View/download PDF
19. Computational attributes of protein kinase-C gamma C2-domain & virtual screening for small molecules: elucidation from meta-dynamics simulations & free-energy calculations
- Author
-
Singh, Harvinder, Raja, Anupam, Shekhar, Nishant, Chauhan, Arushi, Prakash, Ajay, Avti, Pramod, and Medhi, Bikash
- Abstract
AbstractEpilepsy, a moderate to chronic neuropathological condition, is induced by the acute blockage of synaptic and voltage-gated inhibitory conduction or through the activation of synaptic and voltage-gated excitatory conduction. The regulation of long-term potentiation (LTP) is important in the regulation of epileptic events, and its activity is linked to specific protein kinases. The PKC-γ subtype is a vaguely explored therapeutic target for neurological disorders, but in selected studies, it is proven to be a critical intermediate protein in LTP. This study utilized computational modelling approaches including receptor-based docking, QSAR followed by explicit binding score assessment method MM/GBSA, MM/PBSA (EDA) and MTD simulation-based FES iteration. This was performed to virtually screen the small molecule libraries, which comprised about 2.79 lacs compounds against the Ca2+-binding site of the PKC-γ-C2 regulatory domain. The screened molecules LIG-41 ([4-Oxo-4-(4-phenylmethoxyanilino) butyl] azanium) and LIG-16 (Emixustat) exhibit overall optimal attributes in the above-mentioned parameters. The two leads are expected to inhibit the Ca2+-mediated PKC-γ activity.Communicated by Ramaswamy H. Sarma
- Published
- 2023
- Full Text
- View/download PDF
20. Toward carbon-nanotube-based theranostic agents for microwave detection and treatment of breast cancer: enhanced dielectric and heating response of tissue-mimicking materials
- Author
-
Mashal, Alireza, Sitharaman, B., Li, X., Avti, P. K., Sahakian, A. V., Booske, J. H., and Hagness, S. C.
- Subjects
Nanotubes -- Thermal properties ,Breast cancer -- Diagnosis ,Breast cancer -- Care and treatment ,Biological sciences ,Business ,Computers ,Health care industry - Published
- 2010
21. Secretory Phospholipase A2(sPLA2) Isozymes as Potential Targets in Tobacco Condensate- induced Colon Damage
- Author
-
Sharma, Sanjeev K., Yadav, Subodh K., Sharma, Ujjawal, Avti, Pramod, Rana, Satyavati, and Khanduja, Krishan L.
- Abstract
Aims: To find out the role of secretory phospholipase A2 (sPLA
2 ) isozymes as potential targets in tobacco condensate-induced colon damage. Background: The effects of cigarette smoke condensate (CSC) and the molecular mechanisms involved in the regulation of phospholipase A2 (PLA2 ) and its isozymes in colon cells, which are still unclear and emerging, are studied. Objectives: The study aimed to check the effect of CSC on cell viability and reactive oxygen species (ROS) and superoxide. Also, the effect of CSC on gene expression of different secretory phospholipase A2 (sPLA2 ) was evaluated. Moreover, the impact of inhibition of sPLA2 on various cell properties i.e. cell viability, cell proliferation, membrane damage and free radicals’ generation is also studied. Methods: CSC-induced changes were evaluated in cell viability by MTT assay, followed by the evaluation of membrane modulation by flow cytometry, free radical generation by fluorescent dyes, PLA2 isoforms gene expression patterns and their suppression by small interfering RNA (siRNA) studied in HCT-15 male and HT-29 female colon cells. Results: Our results demonstrate that HCT-15 and HT-29 cells treated with CSC significantly reduced the cell viability by 50% within 48 h and significantly enhanced the total reactive oxygen species (ROS) by 2 to 10-fold, and mitochondrial ROS (mtROS) and superoxide radicals (SOR) by 2-fold each. Treatment with CSC significantly unregulated secretory phospholipase A2 (sPLA2 ) IID group and down-regulated IB and cytosolic phospholipase (cPLA2 ) IVA groups in HCT-15 cells without affecting them in HT-29 cells. Silencing the sPLA2 IID group results in an increase in cell viability and a decrease in ROS. Silencing the PLA2 IVA gene in the HCT-15 cells showed a reduced expression which had no impact on the CSC-induced cell proliferation, membrane damage and free radicals (ROS, mtROS, and SOR) generation. Conclusion: Therefore, identifying cell-specific sPLA2 isozymes seems to play a key role in controlling the ROSinduced damage by CSC and helps develop specific therapeutic strategies.- Published
- 2023
- Full Text
- View/download PDF
22. An insight into the simulation directed understanding of the mechanism in SARS CoV-2 N-CTD, dimer integrity, and RNA-binding: Identifying potential antiviral inhibitors
- Author
-
Chauhan, Arushi, Avti, Pramod K., Shekhar, Nishant, Prajapat, Manisha, Sarma, Phulen, Sangwan, Namrata, Singh, Jitender, Bhattacharyya, Anusuya, Kumar, Subodh, Kaur, Hardeep, Sharma, Saurabh, Prakash, Ajay, and Medhi, Bikash
- Abstract
AbstractCoronavirus 2019 is a transmissible disease and has caused havoc throughout the world. The present study identifies the novel potential antiviral inhibitors against the nucleocapsid C-terminal domain that aids in RNA-binding and replication. A total of 485,629 compounds were screened, and MD was performed. The trajectory analysis (DCCM & PCA), structural integrity, and degree of compaction depicted the protein-ligand complex stability (PDB-PISA and Rgyr). Results obtained from screening shortlists 13 compounds possessing high Docking score. Further, seven compounds had a permissible RMSD limit (3 Å), with robust RMSF. Post-MD analysis of the top two compounds (204 and 502), DCCM & PCA analysis show a positive atomic displacements correlation among residues of active sites-dimer (Chain A and Chain B) & residual clustering. The ΔGintof RNA-bound (-83.5 kcal/mol) and drug-bound N-CTD-204 (-40.8 kcal/mol) and 502(-39.7 kcal/mol) as compared to Apo (-35.95 kcal/mol) suggests stabilization of protein, with less RNA-binding possibility. The Rgyrvalues depict the loss of compactness on RNA-binding when compared to the drug-bound N-CTD complex. Further, overlapping the protein complexes (0 ns and 100 ns) display significant changes in RMSD of the protein (204-2.07 Å and 502-1.89 Å) as compared to the Apo (1.72 Å) and RNA-bound form (1.76 Å), suggesting strong interaction for compound 204 as compared to 502. ADMET profiling indicates that these compounds can be used for further experiments (in vitroand pre-clinical). Compound 204 could be a promising candidate for targeting the N-protein-RNA assembly and viral replication.
- Published
- 2022
- Full Text
- View/download PDF
23. Computational basis of SARS-CoV 2 main protease inhibition: an insight from molecular dynamics simulation based findings
- Author
-
Avti, Pramod, Chauhan, Arushi, Shekhar, Nishant, Prajapat, Manisha, Sarma, Phulen, Kaur, Hardeep, Bhattacharyya, Anusuya, Kumar, Subodh, Prakash, Ajay, Sharma, Saurabh, and Medhi, Bikash
- Abstract
AbstractThe coronavirus disease 2019 (COVID-19) pandemic is caused by newly discovered severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2). One of the striking targets amongst all the proteins in coronavirus is the main protease (Mpro), as it plays vital biological roles in replication and maturation of the virus, and hence the potential target. The aim of this study is to repurpose the Food and Drug Administration (FDA) approved molecules via computer-aided drug designing against Mpro(PDB ID: 6Y2F) of SARS CoV-2 due to its high x-ray resolution of 1.95 Å as compared to other published Mprostructures. High Through Virtual Screening (HTVS) of 2456 FDA approved drugs using structure-based docking were analyzed. Molecular Dynamics simulations were performed to check the overall structural stability (RMSD), Cα fluctuations (RMSF) and protein-ligand interactions. Further, trajectory analysis was performed to assess the binding quality by exploiting the protein-residue motion cross correlation (DCCM) and binding free energy (MM/GBSA). Tenofovir, an antiretroviral for HIV-proteases and Terlipressin, a vasoconstrictor show stable RMSD, RMSF, better MM/GBSA with good cross correlation as compared to the Apo and O6K. Moreover, the results show concurrence with Nelfinavir, Lopinavir and Ritonavir which have shown significant inhibition in in vitrostudies. Therefore, we conclude that Tenofovir and Terlipresssin might also show protease inhibition but are still open to clinical validation in case of SARS-CoV 2 treatment.Communicated by Ramaswamy H. Sarma
- Published
- 2022
- Full Text
- View/download PDF
24. Enhancement in alpha-tocopherol succinate-induced apoptosis by all-trans-retinoic acid in primary leukemic cells: role of antioxidant defense, Bax and c-myc
- Author
-
Khanduja, K. L., Kumar, S., Varma, N., Varma, S. C., Avti, P. K., and Pathak, C. M.
- Published
- 2008
- Full Text
- View/download PDF
25. ATRA promotes alpha tocopherol succinate-induced apoptosis in freshly isolated leukemic cells from chronic myeloid leukemic patients
- Author
-
Kumar, Surender, Khanduja, Krishan Lal, Verma, Neelam, Verma, Subhash C., Avti, Pramod Kumar, and Pathak, Chander Mohan
- Published
- 2008
- Full Text
- View/download PDF
26. Fabricating Water Dispersible Superparamagnetic Iron Oxide Nanoparticles for Biomedical Applications through Ligand Exchange and Direct Conjugation
- Author
-
Tina Lam, Pramod K. Avti, Philippe Pouliot, Foued Maafi, Jean-Claude Tardif, Éric Rhéaume, Frédéric Lesage, and Ashok Kakkar
- Subjects
superparamagnetic iron oxide nanoparticles ,nanoparticle ligand functionalization ,contrast agents ,magnetic resonance imaging ,cell internalization ,Chemistry ,QD1-999 - Abstract
Stable superparamagnetic iron oxide nanoparticles (SPIONs), which can be easily dispersed in an aqueous medium and exhibit high magnetic relaxivities, are ideal candidates for biomedical applications including contrast agents for magnetic resonance imaging. We describe a versatile methodology to render water dispersibility to SPIONs using tetraethylene glycol (TEG)-based phosphonate ligands, which are easily introduced onto SPIONs by either a ligand exchange process of surface-anchored oleic-acid (OA) molecules or via direct conjugation. Both protocols confer good colloidal stability to SPIONs at different NaCl concentrations. A detailed characterization of functionalized SPIONs suggests that the ligand exchange method leads to nanoparticles with better magnetic properties but higher toxicity and cell death, than the direct conjugation methodology.
- Published
- 2016
- Full Text
- View/download PDF
27. Detection, mapping, and quantification of single walled carbon nanotubes in histological specimens with photoacoustic microscopy.
- Author
-
Pramod K Avti, Song Hu, Christopher Favazza, Antonios G Mikos, John A Jansen, Kenneth R Shroyer, Lihong V Wang, and Balaji Sitharaman
- Subjects
Medicine ,Science - Abstract
In the present study, the efficacy of multi-scale photoacoustic microscopy (PAM) was investigated to detect, map, and quantify trace amounts [nanograms (ng) to micrograms (µg)] of SWCNTs in a variety of histological tissue specimens consisting of cancer and benign tissue biopsies (histological specimens from implanted tissue engineering scaffolds).Optical-resolution (OR) and acoustic-resolution (AR)--Photoacoustic microscopy (PAM) was employed to detect, map and quantify the SWCNTs in a variety of tissue histological specimens and compared with other optical techniques (bright-field optical microscopy, Raman microscopy, near infrared (NIR) fluorescence microscopy).Both optical-resolution and acoustic-resolution PAM, allow the detection and quantification of SWCNTs in histological specimens with scalable spatial resolution and depth penetration. The noise-equivalent detection sensitivity to SWCNTs in the specimens was calculated to be as low as ∼7 pg. Image processing analysis further allowed the mapping, distribution, and quantification of the SWCNTs in the histological sections.The results demonstrate the potential of PAM as a promising imaging technique to detect, map, and quantify SWCNTs in histological specimens, and could complement the capabilities of current optical and electron microscopy techniques in the analysis of histological specimens containing SWCNTs.
- Published
- 2012
- Full Text
- View/download PDF
28. Integrative Approach to COVID-19: An Indian Facebook Recipe for Mental Health
- Author
-
Anand, Akshay, Tyagi, Rahul, Khosla, Radhika, Bali, Parul, Rain, Manjari, Maity, Kalyan, Verma, Prashant, Gupta, Sheetal J, Nadholta, Pooja, Kaur, Navneet, Sharma, Kiran, Tripathi, Satyam, Avti, Pramod, and Singh, Amit
- Abstract
Background: The COVID-19 pandemic has given the world a big blow and has forced the entire world to develop a new thought process. To cope with the stress of lockdown, it was important for people to indulge in educational and health activities to save them from the threats being caused by the news and social media.Summary: A Facebook page named Yoga scholars Post Graduate Institute of Medical Education and Research (PGIMER) was created where three sessions were held per day for 225 days regularly. This activity resulted in people adopting an integrative approach towards alternative medicine.Key message: This also made possible a trial of Ashwagandha for COVID-19 treatment by the Ayurveda, Yoga, Naturopathy, Unani, Siddha and Homeopathy (AYUSH) ministry.
- Published
- 2021
- Full Text
- View/download PDF
29. In-silico homology assisted identification of inhibitor of RNA binding against 2019-nCoV N-protein (N terminal domain)
- Author
-
Sarma, Phulen, Shekhar, Nishant, Prajapat, Manisha, Avti, Pramod, Kaur, Hardeep, Kumar, Subodh, Singh, Sanjay, Kumar, Harish, Prakash, Ajay, Dhibar, Deba Prasad, and Medhi, Bikash
- Abstract
AbstractThe N terminal domain (NTD) of Nucleocapsid protein (N protein) of coronavirus (CoV) binds to the viral (+) sense RNA and results in CoV ribonucleoprotien (CoV RNP) complex, essential for the virus replication. In this study, the RNA-binding N terminal domain (NTD) of the N protein was targeted for the identification of possible inhibitors of RNA binding. Two NTD structures of N proteins were selected (2OFZ and 1SSK, 92% homology) for virtual screening of 56,079 compounds from Asinex and Maybridge library to identify top 15 hits for each of the targets based on ‘docking score’. These top-hits were further screened for MM-GBSA binding free energy, pharmacokinetic properties (QikProp) and drug-likeness (SwissADME) and subjected to molecular dynamics (MD) studies. Two suitable binders (ZINC00003118440 and ZINC0000146942) against the target 2OFZ were identified. ZINC00003118440 is a theophylline derivative under the drug class ‘bronchodilators’ and further screening with approved bronchodilators was also studied to identify their ability to bind to the RNA binding region on the N protein. The other identified top hit is ZINC0000146942, which is a 3,4dihydropyrimidone class molecule. Hence this study suggests two important class of compounds, theophylline and pyrimidone derivaties as possible inhibitors of RNA binding to the N terminal domain of N protein of coronavirus, thus opening new avenues for in vitro validations. Communicated by Ramaswamy H. Sarma
- Published
- 2021
- Full Text
- View/download PDF
30. Benzo(a)pyrene, an Active Product of Cigarette Smoke, Role in PLA2 Isoforms Activation in Colon Cancer
- Author
-
Sharma, S., primary, Yadav, S., additional, Rana, S., additional, Avti, P., additional, and Khanduja, K.L., additional
- Published
- 2018
- Full Text
- View/download PDF
31. PO-239 Benzo(a)pyrene, an active product of cigarette smoke, role in PLA2 isoforms activation in colon cancer
- Author
-
Sharma, S., primary, Yadav, S., additional, Rana, S., additional, Avti, P., additional, and Khanduja, K., additional
- Published
- 2018
- Full Text
- View/download PDF
32. Activated carbon nanoparticles from biowaste as new generation antimicrobial agents: A review
- Author
-
Lakshmi, S.D., Avti, Pramod K., and Hegde, Gurumurthy
- Abstract
There is a tremendous global threat by the microbes due to their ability to easily migrate and spread in the environment. Alternatively, resistance mechanisms developed by the microbes against the conventional antimicrobial drugs is of great concern due to significant mortality and morbidity and estimates to about 10 million deaths and 100 trillion USD of the global economic burden by the year 2050. WHO’s global surveillance report of 2014 on antibiotic resistance states that at least50% or more of WHO regions including Africa (77%), West Pacific Region (72%), East Mediterranean region (50%), South East Asia (81%) have developed resistance towards various microbes such as Escherichia Coli, Staphylococcus aureus, Klebsiella pneumoniaeetc. This poses various challenges to overcome its pathogenic impacts in biomedical and healthcare sector creating a huge economic burden. In recent years, the synthesis and development of novel and potential antimicrobial agents with high antimicrobial activity is an emerging field of interest. The advent of novel nanomaterials is proven to have potential antimicrobial properties as they efficiently eradicate disease causing pathogens without any side effects due to their unique physico-chemical properties. Among various nanomaterials including carbon, activated carbon based nanoparticles (ACNPs) are emerging as effective antimicrobial agents due to their anti-microbial properties. Development of activated carbon nanoparticles, especially from biowaste derived carbon precursor materials, is a recent upcoming technology due to their easy availability, economic viability and low cost and easy methods of production. This review mainly focuses on the recent trends in the development and use of various activated carbon nanoparticles as anti-microbial agents.
- Published
- 2018
- Full Text
- View/download PDF
33. Serial optical coherence scanning reveals an association between cardiac function and the heart architecture in the aging rodent heart
- Author
-
Castonguay, Alexandre, Lefebvre, Joël, Pouliot, Philippe, Avti, Pramod, Moeini, Mohammad, and Lesage, Frédéric
- Abstract
Normal aging is accompanied by structural changes in the heart architecture. To explore this remodeling, we used a serial optical coherence tomography scanner to image entire mouse hearts at micron scale resolution. Ex vivo hearts of 7 young (4 months) and 5 old (24 months) C57BL/6 mice were acquired with the imaging platform. OCT of the myocardium revealed myofiber orientation changing linearly from the endocardium to the epicardium. In old mice, this rate of change was lower when compared to young mice while the average volume of old mice hearts was significantly larger (p<0.05). Myocardial wall thickening was also accompanied by extracellular spacing in the endocardium, resulting in a lower OCT attenuation coefficient in old mice endocardium (p<0.05). Prior to serial sectioning, cardiac function of the same hearts was imaged in vivo using MRI and revealed a reduced ejection fraction with aging. The use of a serial optical coherence tomography scanner allows new insight into fine age-related changes of the heart associated with changes in heart function.
- Published
- 2017
34. O104. Induction of xenobiotic drug metabolizing enzymes by smokeless tobacco, a culprit of oral cancer
- Author
-
Khanduja, K.L., primary, Avti, P., additional, Pathak, C.M., additional, and Vaiphei, K., additional
- Published
- 2009
- Full Text
- View/download PDF
35. Effect of different doses of Manuka honey in experimentally induced inflammatory bowel disease in rats
- Author
-
Prakash, A., primary, Medhi, B., additional, Avti, P. K., additional, Saikia, U. N., additional, Pandhi, P., additional, and Khanduja, K. L., additional
- Published
- 2008
- Full Text
- View/download PDF
36. Inhibitory effect of vitamin E on proinflammatory cytokines-and endotoxin-induced nitric oxide release in alveolar macrophages
- Author
-
KHANDUJA, K, primary, AVTI, P, additional, and KUMARVANDANAPATHANIACHANDERMOHANP, S, additional
- Published
- 2005
- Full Text
- View/download PDF
37. Low dose gamma-irradiation differentially modulates antioxidant defense in liver and lungs of Balb/c mice
- Author
-
Avti, P. K., primary, Pathak, C. M., additional, Kumar, S., additional, Kaushik, G., additional, Kaushik, T., additional, Farooque, A., additional, Khanduja, K. L., additional, and Sharma, S. C., additional
- Published
- 2005
- Full Text
- View/download PDF
38. Computational investigation of the tumor position and ambient conditions on magnetic nanoparticle thermo-therapy
- Author
-
Nain, Sandeep, Kumar, Neeraj, and Kumar Avti, Pramod
- Abstract
[Display omitted]
- Published
- 2022
- Full Text
- View/download PDF
39. Investigating the correlation between white matter and microvasculature changes in aging using large scale optical coherence tomography and confocal fluorescence imaging combined with tissue sectioning
- Author
-
Farkas, Daniel L., Nicolau, Dan V., Leif, Robert C., Castonguay, Alexandre, Avti, Pramod K., Moeini, Mohammad, Pouliot, Philippe, Tabatabaei, Maryam S., Bélanger, Samuel, and Lesage, Frédéric
- Published
- 2015
- Full Text
- View/download PDF
40. Involvement of Various Molecular Events in Cellular Injury Induced by Smokeless Tobacco.
- Author
-
Pramod K. Avti, Kim Vaiphei, Chander M. Pathak, and Krishan L. Khanduja
- Published
- 2010
- Full Text
- View/download PDF
41. Role of neuroimaging in drug development
- Author
-
Medhi, Bikash, Misra, Shubham, Avti, Pramod Kumar, Kumar, Pardeep, Kumar, Harish, and Singh, Baljinder
- Abstract
AbstractThe development of new molecular imaging techniques has bridged the gap between preclinical and clinical research. During the last decade, the developments in imaging strategies have taken a great leap by the advancements in new imaging scanners, development of pharmaceutical drugs, diagnostic agents, and new therapeutic regimens that made significant improvements in health care. The knowledge gained from imaging techniques in preclinical research can be applicable to the patients. Similarly, the problems from clinical studies with humans can be tested and studied in preclinical studies. The appropriate application of molecular imaging to drug discovery and development can markedly reduce costs and the time required for new drug development. Some imaging techniques, such as computed tomography (CT) or magnetic resonance imaging (MRI), reveal anatomical images, and single-photon emission computed tomography (SPECT), SPECT/positron emission tomography (PET), and PET show functional images. These developing molecular or neuroimaging methods provide increasingly detailed structural and functional information about the nervous system. The basic principles of each technique are described followed by examples of the current applications to cutting-edge neuroscience research. In summary, it is shown that neuroimaging continues to grow and evolve, embracing new technologies and advancing to address ever more complex and important neuroscience questions.
- Published
- 2014
- Full Text
- View/download PDF
42. Multimodal Ultrasound-Photoacoustic Imaging of Tissue Engineering Scaffolds and Blood Oxygen Saturation In and Around the Scaffolds
- Author
-
Talukdar, Yahfi, Avti, Pramod, Sun, John, and Sitharaman, Balaji
- Abstract
Preclinical, noninvasive imaging of tissue engineering polymeric scaffold structure and/or the physiological processes such as blood oxygenation remains a challenge. In vitroor ex vivo, the widely used scaffold characterization modalities such as porosimetry, electron or optical microscopy, and X-ray microcomputed tomography have limitations or disadvantages—some are invasive or destructive, others have limited tissue penetration (few hundred micrometers) and/or show poor contrast under physiological conditions. Postmortem histological analysis, the most robust technique for the evaluation of neovascularization is obviously not appropriate for acquiring physiological or longitudinal data. In this study, we have explored the potential of ultrasound (US)-coregistered photoacoustic (PA) imaging as a noninvasive multimodal imaging modality to overcome some of the above challenges and/or provide complementary information. US-PA imaging was employed to characterize poly(lactic-co-glycolic acid) (PLGA) polymer scaffolds or single-walled carbon nanotube (SWCNT)-incorporated PLGA (SWCNT-PLGA) polymer scaffolds as well as blood oxygen saturation within and around the scaffolds. Ex vivo, PLGA and SWCNT-PLGA scaffolds were placed at 0.5, 2, and 6 mm depths in chicken breast tissues. PLGA scaffolds could be localized with US imaging, but generate no PA signal (excitation wavelengths 680 and 780 nm). SWCNT-PLGA scaffolds generated strong PA signals at both wavelengths due to the presence of the SWCNTs and could be localized with both US and PA imaging depths between 0.5–6 mm (lateral resolution=90 μm, axial resolution=40 μm). In vivo, PLGA and SWCNT-PLGA scaffolds were implanted in subcutaneous pockets at 2 mm depth in rats, and imaged at 7 and 14 days postsurgery. The anatomical position of both the scaffolds could be determined from the US images. Only SWCNT-PLGA scaffolds could be easily detected in the US-PA images. SWCNT-PLGA scaffolds had significant four times higher PA signal intensity compared with the surrounding tissue and PLGA scaffolds. In vivoblood oxygen saturation maps around and within the PLGA scaffolds could be obtained by PA imaging. There was no significant difference in oxygen saturation for the PLGA scaffolds at the two time points. The blood oxygen saturation maps complemented the histological analysis of neovascularization of the PLGA scaffolds.
- Published
- 2014
- Full Text
- View/download PDF
43. Clinically relevant cell culture models and their significance in isolation, pathogenesis, vaccine development, repurposing and screening of new drugs for SARS-CoV-2: a systematic review.
- Author
-
Kumar, Subodh, Sarma, Phulen, Kaur, Hardeep, Prajapat, Manisha, Bhattacharyya, Anusuya, Avti, Pramod, Sehkhar, Nishant, Kaur, Harpinder, Bansal, Seema, Mahendiratta, Saniya, Mahalmani, Vidya M., Singh, Harvinder, Prakash, Ajay, Kuhad, Anurag, and Medhi, Bikash
- Subjects
SARS-CoV-2 ,CELL culture ,VACCINE development ,COVID-19 - Abstract
• Cell lines are best In-Vitro experimental models for standardization and validation of novel and repurposing of drugs. • SARS-CoV-2 entrance is very specific to target cells through ACE2, CD147 and TMPRSS2 receptors. • Our systematic review addresses the utility of SARS COV-2 specific cell lines as potential In-Vitro models. • Elucidated all In-Vitro studies done for SARS-CoV-2 isolation, pathogenesis, and therapeutics. • VeroE6, HEK293, Huh-7 and Caco2 have high ACE2 and TMPRSS2 expression and are potential In-Vitro models for SARS-CoV-2. In-Vitro /Cellular evidence is the backbone and vital proof of concept during the development of novel therapeutics as well as drugs repurposing against COVID-19. Choosing an ideal in-vitro model is vital as the virus entry is through ACE2, CD147, and TMPRSS2 dependant and very specific. In this regard, this is the first systematic review addressing the importance of specific cell lines used as potential in-vitro models in the isolation, pathogenesis, and therapeutics for SARS−COV-2. We searched 17 literature databases with appropriate keywords, and identified 1173 non-duplicate studies. In the present study, 71 articles are included after a careful, thorough screening of the titles and their abstracts for possible inclusion using predefined inclusion/exclusion criteria (PRISMA Guidelines). In the current study, we compiled cell culture-based studies for SARS-CoV-2 and found the best compatible In-Vitro models for SARS-CoV-2 (Vero, VeroE6, HEK293 as well as its variants, Huh-7, Calu-3 2B4, and Caco2). Among other essential cell lines used include LLC-MK2, MDCKII, BHK-21, HepG2, A549,T cell leukemia (MT-2), stems cells based cell line DYR0100for differentiation assays, and embryo-specific NIH3T3 cell line for vaccine production. The Present study provides a detailed summary of all the drugs/compounds screened for drug repurposing and discovery purpose using the in-vitro models for SARS-CoV-2 along with isolation, pathogenesis and vaccine production. This study also suggests that after careful evaluation of all the cell line based studies, Kidney cells (VeroE6, HEK293 along with their clones), liver Huh-7cells, respiratory Calu-3 cells, and intestinal Caco-2 are the most widely used in-vitro models for SARS-CoV-2. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
44. A Novel Nanoparticle-Enhanced Photoacoustic Stimulus for Bone Tissue Engineering
- Author
-
Sitharaman, Balaji, Avti, Pramod K., Schaefer, Kenneth, Talukdar, Yahfi, and Longtin, Jon P.
- Abstract
In this study, we introduce a novel nanoparticle-enhanced biophysical stimulus based on the photoacoustic (PA) effect. We demonstrate that the PA effect differentiates bone marrow-derived marrow stromal cells (MSCs) grown on poly(lactic-co-glycolic acid) (PLGA) polymer films toward osteoblasts. We further show that the osteodifferentiation of the MSCs due to PA stimulation is significantly enhanced by the presence of single-walled carbon nanotubes (SWCNTs) in the polymer. MSCs, without the osteogenic culture supplements (0.01 M β-glycerophosphate, 50 mg/L ascorbic acid, 10−8M dexamethasone), were seeded onto plain glass slides, glass slides coated with PLGA, or glass slides coated with SWCNT-PLGA films and photoacoustically stimulated by a 527 nm Nd:YLF pulse laser, with a 200 ns pulse duration, and 10 Hz pulse frequency for 10 min a day for 15 consecutive days. The study had four control groups; three baseline controls similar to the three experimental groups but without PA stimulation, and one positive control where MSCs were grown on glass slides without PA stimulation but with osteogenic culture supplements. The osteogenic differentiation of all the groups was evaluated using quantitative assays (alkaline phosphatase, calcium, osteopontin) and qualitative staining (alizarin red). After 15 days, the PA stimulated groups showed up to a 350% increase in calcium content when compared with the non-PA stimulated positive control. Further, within the PA stimulated group, the PLGA-SWCNT group had 130% higher calcium values than the PLGA film without SWCNTs. These results were further corroborated by the analysis of osteopontin secretion, alkaline phosphatase expression, and qualitative alizarin red staining of extracellular matrix calcification. The results indicate that PA stimulation holds promise for bone tissue engineering and that the nanomaterials which enhance the PA effect should allow the development of biophysical rather than biochemical strategies to induce osteoinductive properties into tissue engineering scaffolds.
- Published
- 2011
- Full Text
- View/download PDF
45. Whole body exposure to low-dose gamma radiation promotes kidney antioxidant status in Balb/c mice.
- Author
-
Pathak, Chander Mohan, Avti, Pramod Kumar, Kumar, Surender, Khanduja, Krishan Lal, and Sharma, Suresh Chander
- Abstract
We examined the effect of whole body low-dose gamma-irradiation on the status of the antioxidant defense system in the rodent kidneys at different time intervals. Young male Balb/c mice were exposed to whole body radiation from a (60)Co source at doses of 10, 25 and 50 cGy (48.78 cGy/min). Antioxidant status and lipid peroxidation were estimated in the kidneys at 4, 12 and 24 h after irradiation. Lipid peroxidation increased between 33% and 49% and reduced glutathione between 12% and 47% at 12 h at different radiation doses. Reduced glutathione level remained significantly (p < 0.05) elevated even at 24 h after irradiation to 25 cGy. Superoxide dismutase activity also increased by 37% at 12 h on exposure of animals to all the doses up to 50 cGy. Catalase activity increased significantly at 12 h on exposure to 10 cGy and 50 cGy. Interestingly, glutathione peroxidase activity increased by 31% at 4 h and subsequently returned to control levels at 24 h after exposure to 50 cGy. Glutathione reductase activity increased by 10-12% at 12 h after exposure to 25 cGy and 50 cGy. The results suggest that the whole body exposure of animals to gamma radiation stimulates the antioxidant defense system in the kidneys within 4 to 24 h after irradiation, at doses of 25 cGy and 50 cGy.
- Published
- 2007
- Full Text
- View/download PDF
46. Nanobiomaterials: Current status and future prospects
- Author
-
Avti, P. K., Patel, S. C., and Balaji Sitharaman
47. Differential Antifungal Efficiency of Geraniol and Citral
- Author
-
Gaonkar, Roopa, Avti, Pramod K, and Hegde, Gurumurthy
- Abstract
Terpenoids such as geraniol and citral are known to have antibacterial, antifungal and anti-cancerous properties; however, their mechanism of action is least understood. In this study, the antifungal mechanism of monoterpene alcohol (geraniol) and monoterpene aldehyde (citral) was studied using Saccharomyces cerevisiaethroughout 120 h duration and over a maximum tolerable dosage of 0.5% (v/v). Cell growth studies using optical density readings at 600 nm (ODλ=600nm), Cell viability using MTT assay, Na+/K+leakage into media, osmotic stress using flame photometry, detection of metabolites like dehydroergosterol (DHE), H2O2using fluorescence spectroscopy, changes in functional group analysis using FT-IR, elemental analysis using EDAX, DNA damage using gel electrophoresis and cellular structural changes using SEM were carried out. Growth inhibition studies using ODλ=600nmand MTT assays suggest that both geraniol and citral inhibited the growth kinetics significantly up to 0.1% v/v at and significantly increased beyond 0.1% at all the studied time points. Both the monoterpenoids induced the osmotic stress in S. cerevisiaemeasured as a change in reduction in pH, [H]+concentration, with elevation in [Na]+and [K]+leakage into the media. Geraniol treatment reduced the levels of metabolites, dehydroergosterol (DHE) and H2O2, in a time-dependent manner whereas citral only affected their levels at 120 h. Energy dispersive X - ray spectroscopy (EDAX) studies suggest that both the monoterpenoids treatment differentially modulated the cellular elemental contents. Geraniol (0.03% v/v) and not the citral treatment induced DNA damage. Cellular structural analysis showed that both monoterpenoids have differential damaging effects to S. cerevisiae. This study shows that having an aldehyde and alcohol group on the terpenoids strongly affect the anti-fungal activity.
- Published
- 2018
- Full Text
- View/download PDF
48. Effects of anesthesia on the cerebral capillary blood flow in young and old mice
- Author
-
Periasamy, Ammasi, So, Peter T. C., König, Karsten, Moeini, Mohammad, Tabatabaei, Maryam S., Bélanger, Samuel, Avti, Pramod, Castonguay, Alexandre, Pouliot, Philippe, and Lesage, Frédéric
- Published
- 2015
- Full Text
- View/download PDF
49. Role of mind–Body intervention on lipid profile: A cross-sectional study
- Author
-
Priya Mehra, Akshay Anand, R Nagarathna, Navneet Kaur, Neeru Malik, Amit Singh, Viraaj Pannu, Pramod Avti, Suchitra Patil, and H R Nagendra
- Subjects
meditation ,mind–body intervention ,physical activity ,practitioners ,yoga ,Miscellaneous systems and treatments ,RZ409.7-999 - Abstract
Background: Yoga is a combination of physical-breathing and meditative techniques that assist in the unification of the mind–body, which improves the quality of life. It was shown that long-term Yoga practitioners had superior control over respiratory rate, reduced stress and anxiety, and a better-controlled lipid profile. Purpose: We aimed to investigate the lipid profile of long-term yoga practitioners who were practicing yoga for more than 1 year in comparison with the nonyoga group. Methods: A nationwide survey was conducted in which the long-term yoga practitioners (n = 76) and nonyoga practitioners (n = 80) were recruited for assessment for the lipid parameters. Results: The mean (standard deviation) values of both groups were within normal range with serum cholesterol at 189.715 ± 20.4 and 180.88 ± 29.7 and triglycerides at 216.72 ± 92.5 and 207.665 ± 88.3, low-density lipoprotein at 126.65 ± 18.5 and 120.775 ± 26.5, and high-density lipoprotein at 47.17 ± 6.6 and 44.99 ± 7.0, respectively, in yoga and no-yoga groups. Conclusion: The lipid profile values were similar in yoga and nonyoga practitioners in the 2017 survey.
- Published
- 2021
- Full Text
- View/download PDF
50. Can Yogic Breathing Techniques Like Simha Kriya and Isha Kriya Regulate COVID-19-Related Stress?
- Author
-
Manjari Rain, Balachundhar Subramaniam, Pramod Avti, Pranay Mahajan, and Akshay Anand
- Subjects
COVID-19 ,Isha Kriya ,novel coronavirus ,Simha Kriya ,yogic breathing ,breathing techniques ,Psychology ,BF1-990 - Abstract
The global impact of Coronavirus Disease 2019 (COVID-19) is tremendous on human life, not only affecting the physical and mental health of population but also impacting the economic system of countries and individual itself. The present situation demands prompt response toward COVID-19 by equipping the humans with strategies to overcome the infection and stress associated with it. These strategies must not only be limited to preventive and therapeutic measures, but also aim at improving immunity and mental health. This can be achieved by yogic breathing techniques. In this perspective, we emphasize the importance of yogic breathing, Simha Kriya and Isha kriya, the simple yet effective breathing techniques.
- Published
- 2021
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.