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Avicennia officinalis L. is an important mangrove plant used in traditional medicine to treat various diseases like asthma, dyspepsia, paralysis, tumor and rheumatism in aboriginal peoples in Andaman and Nicobar Islands (ANI). The study analyzed and separated bioactive speciation of ethyl acetate leaves extract of A. officinalis from tropical mangrove plants by Gas Chromatography-Mass Spectrometry (GC-MS) and further antimicrobial and antioxidant potential assay. GC-MS results of A. officinalis identified eight different bioactive compounds namely Trans-cinnamic acid, 2,4-Di-tert-butyl phenol, Phenol, 3-(2-phenylethyl, n-hexadecanoic acid, Dibutyl phthalate, Phytol, β – Sitosterol, Androst-7-ene-6, 17-dione and 2,3,14-trihydroxy. The natural products present in A. officinalis showed strong antibacterial activity against different bacteria like gram-positive strain: Streptococcus mutans (19 mm maximum zone of inhibition), Bacillus subtilis (17 mm), Pseudomonas aeruginosa (16 mm), and gram-negative: Klebsiella pneumonia (22 mm) and Enterobacter aerohenes (14 mm). Further, this crude extract showed good antioxidant activity with IC50 values of 23.60±0.43 µg/ml measured using the DPPH method. Thus, the study put forth the promising role of ethyl acetate leaves extract of A. officinalis in the formulation of therapeutic drugs against bacterial infections, antioxidants and cancer.

Mangrove species, viz., Avicennia officinalis, Avicennia marina var. acutissima, Avicennia marina (dwarf), Rhizophora mucronata, Sonneratia alba, Aegiceras corniculatum, Kandelia candel were chosen for measurement of height of the tree and girth or circumference. The sampling was random and at least 50 records were made. The girth is measured by the tape. The measurement of the height is made with the help of abny level. The Tables 1 to 8 records the values for girth, height and canopy cover as well as for correlation coefficient (r). There correlation between girth and canopy in all the species studied however in case of Avicennia officinalis and Aegiceras corniculatum girth and height show more co-relation than girth and C. cover. The positive co-relation observed between girth and canopy is more or less 0.7 except Avicennia marina (dwarf) Excoecaria agallocha and Aegiceras corniculatum. The co-relation is observed in girth and height is difficult to explain. This case is observed in Avicennia officinalis and Aegiceras corniculatum.

This study evaluated anti-bacterial, anti-diarrheal, and cytotoxic activities of ten edible fruits (Aegiceras corniculatum, Avicennia officinalis, Bruguiera gymnorrhiza, Ceriops decandra, Heritiera fomes, Nypa fruticans, Phoenix paludosa, Sarcolobus globosus, Sonneratia caseolaris, and Xylocarpus mekongensis) in the Sundarbans mangrove forest of Bangladesh. Highest antibacterial activity was shown for ethanol:methanol (1:1) extracts of S. caseolaris (2 mg/disc), which demonstrated inhibition zones of 16.7, 17.0, 14.7, 15.7, and 15.7 mm against Escherichia coli, Klebsiella sp., Shigella boydii, Shigella sonnei, and Staphylococcus aureus, respectively. In mice with castor oil-induced diarrhea, S. caseolaris extract (250 mg/kg body weight) showed the highest inhibition (87.7%) and delayed the onset time (273 min) of diarrheal episodes, followed by A. corniculatum extract (inhibition, 83.6%; delayed onset time, 187.4 min). S. caseolaris and A. corniculatum fruit powders were successively fractionated into n-hexane, diethyl ether, chloroform, ethanol, and methanol. Antidiarrheal activity significantly increased with increasing polarity of these fractions. In brine shrimp lethality assay, S. globosus extract demonstrated the highest cytotoxicity (59.2 μg/mL), followed by H. fomes (74.1 μg/mL) and C. decandra (116.7 μg/mL); however, lowest cytotoxicity was shown for A. corniculatum, A. officinalis, and S. caseolaris extracts. Mice treated with A. corniculatum extract did not experience any acute toxicity. These results demonstrate that S. caseolaris and A. corniculatum fruits have potential to treat diarrhea and may act as useful nutraceuticals.

Banyuwangi is an area that has the longest coastline in East Java, with a length of 175.8 km. one of the coastal potentials is the mangrove ecosystem. Pantai Sari is one of the locations that has the potentials to be developed in Banyuwangi regency. This beach is a forest area in the city of Banyuwangi. In general, they have natural charateristics, many pine trees have been planted since 2015. The purpose of this study was to obtain data on the diversity of mangroves in river and coastal areas at Pantai Sari. The method used is descriptive quantitative, with data collection using nested plot method with a distance of 20 m between plots. The results showed that there are 7 species mangroves were found in the river, namely Avicennia officinalis , Rhizophora apiculata , Sonneratia caseolaris , Lannea coramandelica , Erythrina costaricensis , dan Thespesia populnea . The highest number of individuals was Rhizophora apiculata with number 1,291 individu, and the lowest number was Erythrina costaricensis and Thespesia populnea . The other hand, there was 5 species mangroves in the coastal area, namely Casuarina cunninghamiana , Casuarina equisetifolia , Lannea coramandelica , Terminalia catappa , dan Thespesia populnea . The highest number of individuals was pine beach ( Casuarina equisetifolia ) with number 122 individu, and the lowest number was waru laut ( Thespesia populnea ) with number 1 individu

The Sundarbans is a protected forest ecosystem in Bangladesh. A large number of people depend on this forest for their livelihoods and income. Many farmers in the region are now adopting fish farming—specifically, aquasilviculture (multi-purpose production system that allows production of fish and perennial plants mostly trees), one of the most profitable agroforestry systems in many countries. The present study aimed to study the status, benefits, and farmers’ perceptions of and attitudes towards the practice of aquasilviculture in the periphery of the Sundarbans. It was conducted in the Shyamnagar upazilla (administrative district) in the southwestern part of Bangladesh, which is recognized as the most vulnerable upazilla on the coast. About 10% of the area’s shrimp farms were randomly selected and their farmers were interviewed using a semi-structured questionnaire. Most farmers (87%) had a positive attitude toward and clear perception of aquasilviculture practices. Farmers also perceived that trees helped increase their income and provide sources of fuelwood, timber, and fodder for their livestock. The respondents mainly preferred to plant Albizia procera, Acacia nilotica, Azadirachta indica, Psidium guajava, Excoecaria agallocha, and Avicennia officinalis on the dikes of fishponds. Farmers considered increased levels of salinity in water and soils, lack of access to capital, and inadequate technical assistance the main barriers to the practice of aquasilviculture in the region. Aquasilviculture could be an environmentally friendly and climate-smart aquaculture practice in the area, one that increases green coverage on fishponds’ dikes while also improving the livelihoods of local farmers.

The aim of this study was to assess probabilistic human health risk due to ethnobotanical usage of Avicennia officinalis, Porteresia coarctata and Acanthus ilicifolius. The study was conducted at the tannery outfall near Sundarban (Ramsar wetland, India) mangrove ecosystem affected by potentially toxic elements (Cd, Cr, Cu, Hg, Mn, Ni, Pb, and Zn). Total metal concentrations (mg kg-1) were considerably higher in the polluted rhizosphere namely, Cd (1.05–1.97), Cu (36.3–38.6), Cr (144–184), Hg (0.04–0.19), Mn (163–184), Ni (37.7–46.4), Pb (20–36.6), and Zn (97–104). Ecological risk index indicated low to moderate ecological risk in this site, whereas the ecological risk factor showed high potential ecological risk due to Cd pollution. BCR Sequential extraction of metals showed more exchangeable fraction of Cd (47–55%), Cr (9–13%), Hg (11–13%), and Pb (11–15%), at the polluted site. Mercury, though present in trace amount in sediment, showed the highest bioaccumulation in all the three plants. Among the toxic trio, Hg showed the highest bioaccumulation in A. officinalis, Cd in P. coarctata but Pb has the lowest bioaccumulation potential in all the three species. Occasional fruit consumption of A. officinalis and dermal application of leaf, bark of A. officinalis (antimicrobial), A. ilicifolius (anti-inflammatory, pain reliever when applied on wounds) indicated negligible human health risk. However, long-term consumption of P. coarctata (wild rice variety) seeds posed health risk (THQ>1) both in adults and children age groups. This study concludes that nature of ethnobotanical use and metal contamination levels of the mangrove rhizosphere can impact human health. The transfer process of potentially toxic elements from rhizosphere to plants to human body should be considered while planing pollution mitigation measures.

In a tropical coastal environment, mangrove ecosystems store large amount of atmospheric carbon in biomass and soil. West coast of India host 29% of the mangrove ecosystems of the country. Putting aside the few scientific studies about the Indian mangroves system, there exist gaps in our scientific knowledge of Blue Carbon domain. This study aims to quantify the ecosystem carbon stocks potential and community structure of mangrove vegetation/ ecosystem species of Vypin– Cochin, a coastal region of the Southwest coast of India. The information on species-specific carbon storage will benefit to choose best species in the afforestation and conservation initiatives. Distribution, community structure and carbon pools of above and below ground biomass of the study area were assessed. From the analysis, it is evident that the amount of carbon stored in mangroves varies according to species, functional attributes (basal area, DBH, mean height etc.) and region. Avicennia officinalis, Rhizophora mucronata, Acanthus ilicifolius, Acrostichum aureum, Bruguiera cylindrica and Sonneratia caseolaris are the mangrove species observed from the study area. As per the community structure assessment, A. officinalis showed large number, frequency, density, and evenness. While considering biomass, R.mucronata species possess high value. The mean overall above ground biomass of the region was 69.55 ± 80.38 t ha−1. Ecosystem carbon stock of the area ranges from 17.7–138.8 with an average of 54.3 ± 36 t ha−1. Therefore, the findings described will serve as a baseline on carbon stock and mangrove stand structure of the region and their role in Blue Carbon dynamics of the Southwest Coast of India.

Mangroves adaptive plasticity in the changing environmental conditions is of vital importance for conservation management. Genetic diversity of mangrove brings about adaptive plasticity, enabling a species to cope with different habitat conditions. The Sundarbans of Bangladesh is the largest coastal wetland and mangrove forest with diversified habitat conditions that support a wide diversity of flora and fauna. Avicennia officinalis L. is the pioneer species in mangrove succession in the low-salinity, medium-salinity and high-salinity zones of the Sundarbans. Adopting RAPD-PCR analysis, the genetic diversity of A. officinalis was studied to explore the ecotypes of this species in the Sundarbans. The genetic distances of A. officinalis between the low-salinity and medium-salinity zones (0.50) and between the low-salinity and high-salinity zones (0.52) are significantly (p

Naturally grown mangrove forest is the south coastal community’s green barrier from any type of hydro-meteorological hazard and disasters. South-western coastal area of Bangladesh is covered by the Sundarbans, and mid-central zone is covered by Tengragiri wildlife sanctuary which is taken as a protected area in this recent era but not mapped properly with considering different species diversification and temporal changes pattern which is required for successful co-management of this mangrove forest. This study identifies species composition, plant diseases, degradation of the mangrove forests and further focuses on mapping mangroves by conducting plotting-based primary field visit, variability analysis using different indices and cross-matching with secondary databases. Existing mangrove forest boundary’s 200 m buffering with three-time series satellite imagery 2000, 2010 and 2017 is considered for further analysis. Including all buffering zone, this protected area considered 9 major classes that included four subclasses for presenting result like Baen (Avicennia officinalis), Gewa-Goran (Excoecaria agallocha, Ceriops decandra), Keora (Sonneratia apetala), Sundri (Heritiera fomes), Plantation trees Samanea Saman (Raintree), agriculture-grassland (agri-grass) and homestead settlement, sandbar and waterbody. Mapping accuracy assessment purpose automatically generated 996 points cross-matched with previously mangrove species level detailed survey results and found highest accuracy in Sundri species (70%) and all others above 50%. During 2000–2017, the Keora area showed the highest increase 129% over 2000 and increasing rate 13.17 ha/yr. About 26% Sundri and Baen–Passur increased around 13.45 ha/yr. In case of Sundri, 70% area coverage remained intact during 2000–2017, while other 25% classified in 2017 as Avicennia officinalis, Gewa-Goran classes. Furthermore, using refracted electromagnetic energy from various physical characteristics of plants application, four indices (NDVI, DVI, MSAVI-2, RVI) are usable where single-species-level crop density analysis has limitations, but identification of Sundri and Keora by MSAVI-2 and NDVI found significant and alternately lower accuracy values from RVI. Identification of dominant mangrove species groups as well as area gains and losses over 2000–2017 is a robust biophysical baseline for management of the sanctuary. Natural hearts of this area and working as first-step warriors against natural disasters originated from Indian Ocean and Bay of Bengal; so far, it is very much required to save this forest and the coastal communities as well. The results of the study and maps will be helpful for the scientific community, planners, government-international bodies and the activists, Forest Department and the local community in effective planning, monitoring the effectiveness of co-management in conservation of the sanctuary.

In Myanmar’s Ayeyarwady Delta, mangrove forests were widely converted to paddy fields, then abandoned. However, the mangrove ecosystems often fail to recover. To identify the mangrove restoration options in these fields, we conducted the current study in Pyindaye Forest Reserve, Pyapon Township of the Delta by examining the structural and functional responses to restoration for two common mangrove species (Avicennia officinalis and Bruguiera sexangula) after three post-abandonment periods (4, 8, and > 10 years), the natural recovery after these periods, and management to improve degraded forests dominated by the two species. Without replanting, we observed no mangrove cover or regeneration more than 10 years after abandonment. The sites were dominated by a perennial grass (Paspalum vaginatum), sparse climber, herbs, and spiny shrubs. With increasing the abandoned period, we observed decreasing soil quality and increasing vegetation cover by the grass, herbs, and spiny shrubs in response to increasingly harsh micro-environmental conditions for natural recovery of mangrove. Without human assistance, the abandoned paddy fields will not recover their productivity after more than 10 years. However, with increasing the period of establishing mangrove plantations in the abandoned fields, the ecosystem showed improved vegetation structure and regeneration of both planted and non-planted species. The soil conditions improved during mangrove recovery, and a well-developed canopy and aerial root system developed. By 8 years after mangrove establishment, the land could be used again as paddy fields due to suppression of P. vaginatum and the improved micro-environment. The managed forests showed the best structural attributes, conditions for regeneration, and micro-environmental conditions for plant growth and regeneration. Our results suggest three options for managing the abandoned paddy fields: paddy cultivation based on fallow periods with mangrove establishment, mangrove planting for wood production, and mangrove planting for environmental restoration.

The mangroves of Andaman and Nicobar Islands are considered as one of the finest vegetation in India with respect to species composition as well as structure. However scientific information on the influence of edaphic attributes on the structure of Andaman mangroves are lacking. Hence, we assessed the floristic composition, structural attributes, zonation pattern and its relation to edaphic variables of mangroves of the Andaman islands. Quadrat method was employed to estimate the different structural variables and multiple regression analysis to explain the influence of edaphic variables over structural attributes. Floristic analysis of 420 quadrats (10x10m) revealed a mixed species composition with 28 true mangroves in Andamans, of which Avicennia officinalis, Rhizophora apiculata, and R. mucronata were designated as the most important species based on the importance value index. Stem density varied from 133 to 14,000 ha−1 and individual basal area 0.07–78.75 m2 ha−1, further, most of the mangrove habitats showed very high stand basal area. Relatively higher Maturity Index Value was observed indicating high structural maturity in Andamans. Species zonation pattern typically varies based on the tidal inundation. Multiple regression analysis revealed the importance of soil pH, silt, total carbon, total Nitrogen and salinity in shaping the structure of Andaman mangroves. The data evolved in the study would be valuable in the site-specific management and conservation approaches of mangroves in Andamans.

Mangrove sediment acts as a natural accumulator of potentially toxic metals (PTMs). Here, we have investigated the efficiency of mangrove species, e.g., Sonneratia caseolaris and Avicennia officinalis phytoremediation potential in a globally significant vulnerable river. The lower pH and anoxic conditions facilitate the elevated accumulation of PTMs in river Hooghly. The presence of PTMs in fine sediment fraction in greater proportion indicates a larger role of fine-grained silt and clay particles in the accumulation process. The estimated sediment quality indices indicate no significant change in the last 30 years. However, the ecotoxicological indices suggest a low level of ecological risks but can turn toxic because of the gradual accumulation of metals. The accumulations of PTMs in mangroves are regulated by the metal bio-availability. The result emphasizes mangrove pneumatophores, as a greater accumulator of PTMs than mangrove leaves. Higher translocation factors also indicate the applicability of mangroves as a phytoremediator of contaminated sediment.

Natural ecosystems are highly dynamic and exhibit a highly nonlinear nature. We attempt to detect and analyze changes in a coastal mangrove ecosystem to predict the dynamics of the system and its biodiversity. Multitemporal hyperspectral data have been used to analyze the competition among mangrove and saline blank endmembers and their dominance with time. We aim to predict the ecodynamics of the area through subpixel analysis of multitemporal hyperspectral imagery. The biodiverse coastal zone of Sunderban Biosphere Reserve, West Bengal (a world heritage site), is considered as a case study for predicting the mangrove ecodynamics of the area through Markov chain analysis. The mangrove species of Sunderban vary in their abundance with time due to dynamic weather conditions. The model is applied to hyperspectral data from 2011 and 2014, collected over Henry Island in the Sunderban to predict species dynamics in 2017 and 2020. An endmember transition matrix is framed to determine the endmember dynamics in the area in terms of degradation and regeneration of mangroves and saline blank cover in the area. Based on the transition probability matrix, the abundance values have been predicted for 2017 and 2020. The predicted abundance values have been validated by ground truth values extracted during field visits in 2017. It is observed that, in certain locations, the increase in saline blanks has led to an overall decrease in the proportion of Phoenix paludosa and Ceriops decandra (a salt-intolerant mangrove species) over the years. However, there is an increase in Avicennia marina and Avicennia officinalis, which are salt tolerant and can sustain in extreme saline conditions.

The present study was aimed at evaluating the antidiabetic activity of the ethanol bark extract of a mangrove plant Avicennia officinalis (AOEB) in streptozotocin (STZ)-induced diabetic mice. The study revealed AOEB could lower the blood glucose level significantly (p

The secondary metabolite 2,4,6-triphenylaniline (TPA) was isolated from an endophytic fungi Alternaria longipes strain VITN14G of mangrove plant Avicennia officinalis, that exhibited satisfactory in vitro antidiabetic activity for type 2 diabetes mellitus (T2DM). The TPA was encapsulated using nanoemulsion (NE) to overcome the problem of stability and permeability to increase its therapeutic applications. Response surface methodology (RSM) was used for the optimization of the variables given, such as hydrodynamic diameter, surface charge, and polydispersity index (PDI). TPA was encapsulated using an optimized ratio of olive oil and tween 80 (2:1) significantly affected the response variables including particle size (124.8 nm), ζ potential (-46.0 mV), and PDI (0.396), and the encapsulation efficiency was found to be 95.93%. The TPA-loaded NE after MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) analysis showed nontoxic effects on L929 normal cell lines (areolar and adipose subcutaneous connective tissue of Mus musculus) with a viable percentage of 92%. In vitro release study revealed the slow and sustained release of the TPA over 48 hrs from NE under the Fickian diffusion mechanism and followed the Higuchi model for release kinetics. Further, the percentage of α-glucosidase and α-amylase inhibition rate of TPA-loaded NE was found to be 78.5 and 43.42%, respectively. The present study, therefore, can aid in the development of a novel drug delivery system as a therapeutic approach to T2DM.

The present study involves biosynthesis of AgNPs using Avicennia officinalis and Xylocarpus granatum mangrove plants along with evaluation of their potential biomedical applications. The synthesized AgNPs were characterized by UV–Vis spectroscopy, FTIR analysis, scanning electron microscope, particle size analyzer, X-ray diffraction (XRD). The synthesized AgNPs showed absorption maxima at 470 nm for X. granatum (XG-AgNPs) and 420 nm for A. offcinalis (AO-AgNPs) which corresponds to their respective surface Plasmon resonance. The FTIR analysis reveals capping of phenolic groups providing stability of synthesized AgNPs. The morphology of silver nanoparticles was confirmed by SEM technique. The dynamic light scattering study (DLS) also confirmed the size distribution of synthesized AgNPs. XRD peaks at 2θ range of 20–70o corresponds (111), (200) and (220) reflection planes indicating the structure of metallic silver. AO-AgNPs exhibited better DPPH scavenging, superoxide and protein denatuaration activity with IC50 values of 0.14, 0.32 and 0.21 mg/ml. However, XG-AgNPs exhibited better α-amylse and α-glucosidase inhibition potential as compared to AO-AgNPs. It could be concluded that X. granatum bark extracts and A. officinalis leaf extract can be used efficiently for the synthesis of biologically active silver nanoparticles which could be exploited pharmaceutical applications.

An experiment was conducted to assess the effect of NPK fertilizers on seedling growth and survival of five important mangrove species of Bangladesh for nine months. Seedlings were raised in the nursery using plastic pots. NPK fertilizers with three treatments (control, 4 gm and 8 gm doses) were used two times when seedlings age was 3 and 6 months. The result showed that seedlings height growth of Excoecaria agallocha was enhanced significantly with the application of NPK fertilizers but it was shown negatively significant effect on height growth of Xylocarpus mekongensis after second time fertilizer application. Moreover, NPK application enhanced mortality of Avicennia officinalis and Ceriops decandra seedlings in both times of fertilizer application. But no effect of NPK fertilizers on seedlings growth and survival of Heritiera fomes were observed.

The recent coronavirus disease 2019 (COVID-19) pandemic is a global threat for healthcare management and the economic system, and effective treatments against the pathogenic severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus responsible for this disease have not yet progressed beyond the developmental phases. As drug refinement and vaccine progression require enormously broad investments of time, alternative strategies are urgently needed. In this study, we examined phytochemicals extracted from Avicennia officinalis and evaluated their potential effects against the main protease of SARS-CoV-2. The antioxidant activities of A. officinalis leaf and fruit extracts at 150 µg/mL were 95.97% and 92.48%, respectively. Furthermore, both extracts displayed low cytotoxicity levels against Artemia salina. The gas chromatography–mass spectroscopy analysis confirmed the identifies of 75 phytochemicals from both extracts, and four potent compounds, triacontane, hexacosane, methyl linoleate, and methyl palminoleate, had binding free energy values of −6.75, −6.7, −6.3, and −6.3 Kcal/mol, respectively, in complexes with the SARS-CoV-2 main protease. The active residues Cys145, Met165, Glu166, Gln189, and Arg188 in the main protease formed non-bonded interactions with the screened compounds. The root-mean-square difference (RMSD), root-mean-square fluctuations (RMSF), radius of gyration (Rg), solvent-accessible surface area (SASA), and hydrogen bond data from a molecular dynamics simulation study confirmed the docked complexes′ binding rigidity in the atomistic simulated environment. However, this study′s findings require in vitro and in vivo validation to ensure the possible inhibitory effects and pharmacological efficacy of the identified compounds.

Mangrove forests are ecosystems that make up coastal areas and river estuaries. The examples of mangrove ecosystems found in Indonesia are in Segara Anakan, Central Java. One of the aquatic biota with the economic value found in the mangrove forest ecosystem is the mud crab (Scylla spp.). The purpose of this study was to assess the distribution of mangrove crabs (Scylla spp.) to the presence of mangrove species in the mouth of the Donan River, Segara Anakan. A sampling of crabs and observations of mangrove vegetation were carried out at five stations spread from the river to the sea. The results showed that there were three types of mangrove crabs caught in the vicinity of the study, namely Scylla tranquebarica, S. olivacea, and S. serrata. The types of mangroves found include Rhizophora apiculata, R. mucronata, Avicennia rumphiana, A. alba, A. officinalis, and Nypa. Regression analysis showed that mangrove density correlated with the abundance of Scylla tranquebarica and Scylla olivacea, but contradicts the abundance of Scylla serrate. PCA analysis showed that the Crab species Scylla tranquebarica and Scylla olivacea were associated with mangroves of Avicennia alba, Avicennia rumphiana, and Rhizophora apiculata. Meanwhile, Scylla serrata crabs are associated with Avicennia officinalis.

Brownies is a popular high-calorie snack. In this studied, brownies were processed from several types of flour, namely pedada fruit (Sonneratia caseolaris) flour, api-api (Avicennia officinalis) flour, red bean flour, and modified cassava flour with nutritional value and health benefits compared to the commercial ones (from wheat flour). The purpose of this studied was to determine the used of a variety of superior flours as raw material for making brownies in terms of nutritional value and leveled of preference. The experimental design used a completely randomized design (CRD) with test parameters including the leveled of preference, caloric value, fiber, and proximate composition. The results of the studied were tested for variance using spss version 22 software with a 95% confidence interval. The results showed that the best results were brownies made from pedada fruit flour because they had higher fiber and lowered calories with test scores for calorie content, crude fiber, air content, fat content, protein content, ash content, carbohydrate content respectively. 401. 87 kcal each; 16.49%; 18.32%; 21.7%; 4.03%; 1.44%; and 54.64%.

The species composition and geospatial distribution of coastal ecosystems such as mangroves, mudflats, filtration ponds, backwaters, and so on and their associates in the southwest coast of India are studied through field mapping using high-precision GPS and incorporating information extracted from high-resolution world-view satellite images. Appropriate management strategies to protect and restore the mangrove and mudflat ecosystems under the provisions of Coastal Regulation Zone (2011) (INDIA) are discussed. The Kerala coast, located at the southwest peninsula of India, has one of the longest densely populated rural–urban agglomerated coastal plains in India. Being one of the highly populated states of India, its low-lying coastal areas (2.42% of its total geographical area) includes 8.25% of its total population, with an average coastal density of 2931/ km2. Kerala coastal plains were reported for 10 exclusive mangrove species: Avicennia officinalis, Avicennia marina, Rhizophora mucronata, Sonneratia alba, Acanthus ilicifolius, Excoecaria agallocha, Bruguiera gymnorrhiza, Lumnitzera racemosa, and Kandelia candel, Barringtonia are reported in the study area. Mangrove coverage in the Alappuzha district of Kerala is 1.43 km2. Spread of mangroves in the two major urban centers, Kochi corporation, Maradu municipality, and adjoining Vembanad, is 0.48 and 0.40 km2, respectively. Coverage of mangroves in Ashtamudi and a few other backwaters is 0.95 km2. Reclamation and destruction of coastal ecosystems have been taking place on a large scale in the urban areas of Kochi corporation and Maradu municipality. Proper intervention through a sustainable management plan like the Coastal Regulation Zone Notification of India will address the issue of conserving the mangroves and associated eco-morphological systems.

Shrimp based mangrove-aquaculture (silvo-aquaculture) is practiced in many countries of the world and leaf litter of different mangrove tree species is a potential nutrient source in these systems. The present study evaluated the effects of mangrove leaf litter from four mangrove species (Sonneratia apetala, S. caseolaris, Avicennia officinalis and Heritiera fomes) on the production of juvenile shrimp (Penaeus monodon) with and without supplemental feed. Fifteen-day-old post larvae (PL15) with an average weight of 0.01 g were reared in 1100 L fibre-reinforced polyethylene tanks containing 1000 L of 10 ppt saline water and a water depth of 0.9 m. Leaf litter with or without supplemental feed was applied to the tanks according to a 4 × 2 factorial design. The PLs were stocked at a density of 100 per tank and the experiment was conducted for 4 weeks without any exchange of water. Both mangrove species and feed application affected shrimp performance and water quality parameters except dissolved oxygen (DO), chemical oxygen demand (COD) and zoo-plankton concentration. The average survival rate of juvenile shrimp ranged from 86 to 94% in the treatments with both leaf litter and feed, 75–82% in the treatments with only leaf litter and 88% in the treatment with only feed. However, 100% mortality was observed in the treatment without any leaf litter or supplemental feed. Combined, leaf litter and feed resulted in 21 to 33% higher weight gain of shrimp PL than based on the combined contributions of leaf litter only or feed only, indicating synergism. Among the different mangrove species, S. apetala (23.1%) contributed the highest to total weight gain followed by A. officinalis (21.6%), S. caseolaris (21.6%) and H. fomes (10%). The lower feed conversion ratio (FCR) (0.18–0.27) in the treatments combining leaf litter and supplemental feed as compared to the feed-only treatment (0.41) indicated that leaf litter (directly or by stimulating natural food production) contributed to supplemental feeding. The growth of phytoplankton also appeared to contribute in low FCR as evidenced by a positive correlation (P < 0.001, r = 0.681⁎⁎) between phytoplankton concentration and shrimp weight gain. The synergistic effect between leaf litter and supplemental feed can help the farmer to minimize the shrimp production cost by lowering the feed input and enhancing mangrove three coverage on pond dikes as an inexpensive source of natural food.

Urban development is driving the loss and subsequent fragmentation of mangrove forests in Penang, Malaysia. However, little research has focused on the implications of urbanization onto the biodiversity within mangrove forests. This study investigates and compares both vegetation and crab assemblages between urban and rural mangrove forests. Collectively, 5853 trees were recorded including 15 species from six families: Acanthaceae, Arecaceae, Lythraceae, Malvaceae, Meliaceae, and Rhizophoraceae. Avicennia marina accounted for 88.6% of all trees in the study and held the highest species importance (IV). Although the total number of mangrove tree species varied significantly between urban (14) and rural (7) mangrove forests, the mangrove forest structure based on IV did not vary significantly. In total, 633 crabs were recorded comprising 13 different species from three superfamilies: Grapsoidea, Ocypodoidea, and Paguroidea. Crab species richness did not vary between urban and rural sites, but the biomasses of five and the densities of four crab species varied significantly. Redundancy analysis (RDA) indicated that land use, salinity, and abundance of Avicennia officinalis can best describe the distribution of crab species (r2 =32.7). This study indicates that smaller fragmented urban mangroves at the east coast of Penang have a relatively high tree and crab diversity and are worth protecting, in addition to the larger mangrove forests on the west coast. The preservation of small yet interconnected mangrove sites could be critical to the recruitment of their flora and fauna and should be considered in decision-making processes.

Salinity is an environmental stress that causes decline in crop yield. Avicennia officinalis and other mangroves have adaptations such as ultrafiltration at the roots aided by apoplastic cell wall barriers to thrive in saline conditions. We studied a cytochrome P450 gene from A. officinalis, AoCYP94B1, and its putative ortholog in Arabidopsis (Arabidopsis thaliana), AtCYP94B1, which are involved in apoplastic barrier formation. Both genes were induced by 30 min of salt treatment in the roots. Heterologous expression of AoCYP94B1 in the atcyp94b1 Arabidopsis mutant and wild-type rice (Oryza sativa) conferred increased NaCl tolerance to seedlings by enhancing root suberin deposition. Histochemical staining and gas chromatography-tandem mass spectrometry quantification of suberin precursors confirmed the role of CYP94B1 in suberin biosynthesis. Using chromatin immunoprecipitation and yeast one-hybrid and luciferase assays, we identified AtWRKY33 as the upstream regulator of AtCYP94B1 in Arabidopsis. In addition, atwrky33 mutants exhibited reduced suberin and salt-sensitive phenotypes, which were rescued by expressing 35S::AtCYP94B1 in the atwrky33 background. This further confirmed that AtWRKY33-mediated regulation of AtCYP94B1 is part of the salt tolerance mechanism. Our findings may help efforts aimed at generating salt-tolerant crops.

Sabdanawaty FP, Purnomo, Daryono BS. 2021. Species diversity and phenetic relationship among accessions of api-api (Avicennia spp.) in Java based on morphological characters and ISSR markers. Biodiversitas 22: 193-198. Api-api (Avicennia spp.) from mangrove groups is rich in economic and ecological benefits. Research on the potential of api-api has been extensively done, but no research is specifically focused on the identification of api-api. This study aims to identify species diversity and phenetic relationships of api-api in Java based on morphological and molecular characters. The morphological analysis was based on 35 characters and referred to the descriptor. The PCA analysis on the morphological characters of Avicennia spp. showed a high diversity. Morphological characters have a high effect on grouping patterns such as habitat, leaf shape, leaf tip, leaf base, and petal color. Molecular observations were done on the polymorphism of DNA bands. The ISSR primers used were ISSR02, ISSR04, and ISSR10. PCR amplification of DNA was separated and visualized using a doc gel electrophoresis. The results showed a high diversity based on the percentage of DNA polymorphism. The research resulted in three species of Avicennia spp. in Java, namely Avicennia officinalis, A. alba, and A. marina. Phenetic relationships between Avicennia spp. form was based on similarity in character, not based on the area of origin. The environmental conditions in this study slightly opposed the character of Avicennia spp., which presumably due to the similarity in various marine areas of Java such as temperature, pH, and the required salinity.

Mangroves are distributed along both the east and the west coasts of India, with more diversity reported from the west coast. More than 414 fungal species belonging to around 226 genera have been reported from the mangroves of India. The majority of them (325 species in 189 genera) belong to Ascomycota, and of those remaining, 35 species in 28 genera belong to Basidiomycota, and 54 species in 10 genera belong to Zygomycota. More than 80% of these fungi could be considered as obligate marine fungi, whereas several facultative marine fungi that are normally recorded from terrestrial environments were also recorded. While 306 fungi belonging to 170 genera were recorded from mangroves of the Indian west coast, 225 species in 131 genera were recorded from mangroves of the east coast. Of the 414 fungi reported from mangroves, 117 species in 75 genera were common to both the east and the west coasts. A total of 108 species in 56 genera were reported just from the east coast Indian mangroves. Likewise, a total of 189 species in 95 genera were recorded just from the west coast of Indian mangroves. From Zygomycota, Glomus was found to be the most speciose genus, having 20 species. Among the different fungal genera of Ascomycota, Corollospora is represented with 13 species, followed by Aspergillus and Aniptodera with 11 and 9 species, respectively. Phaeosphaeria, Leptosphaeria, and Lulworthia are represented with eight species, while many other genera of this group were recorded with fewer species. Among the anamorphic genera, Cirrenalia is found to be represented with six species. Among the different mangrove host plants prevailing in India, Rhizophora mucronata exhibited a large fungal species diversity, followed by Avicennia officinalis, A. marina, and Rhizophora apiculata. Moderate diversity has been reported on Brugueira gymnorrhiza, Sonneratia caseolaris, while other plants, such as Acanthus ilicifolius, Avicennia alba, Ceriops decandra, and so on, have shown a very low fungal diversity.

The purpose of this work was to assemble information with which to help identify and select mangrove species most likely to be useful and locally acceptable for use in transforming the dominant current non-mangrove shrimp culture practice in Bangladesh towards more sustainable shrimp silvo-aquaculture in the coastal regions of the country. We reviewed current mangrove use in the extensive aquaculture setting, compiled published evidence on mangrove properties and characteristics that influence their suitability for such use and assessed farmer preferences of the various species. Thirteen mangrove species (all of which are available in Bangladesh) were documented as being used in silvo-aquaculture system in the tropics. Followed by these already “established” mangrove species, Aegialitis rotundifolia, Heritiera fomes and Lumnitzera racemosa could be enlisted as additional “optional” mangrove species to consider, while Ceriops decandra, Excocaria agallocha and Phoenix paludosa were found to be unsuitable for silvo-aquaculture. Based on their experience and indigenous knowledge on mangroves, shrimp farmers ranked (in declining order of preference) Sonneratia apetala, S. caseolaris, Avicennia officinalis, Nypa fruticans, Bruguiera sexangula, Heritiera fomes, and also, the mangrove-associate wild rice species Oryza coarctata as the most suitable species to be used for promoting silvo-aquaculture in the coastal areas of Bangladesh. Thus, the focus group interviews with the farmers not only identified which mangroves enjoy most local support for use in transforming current shrimp culture towards more sustainable shrimp silvo-aquaculture but also provided additional leads for species interesting to further investigation into their suitability for silvo-aquaculture.

The ability of mangrove trees to reproduce and disperse in highly dynamic tidal environments is reflected in the contemporary genetic diversity and structure of their populations. Resilience and establishment may occur through either local recruitment (propagule pool model) or over longer distances with mixed origins (migrant pool model), thereby affecting the connectivity between natural populations. Anthropogenic activities such as encroachment and fragmentation or from mitigating reforestation could cause strategy switches and thus the connectivity and genetic structure of populations. In this study, we considered Avicennia officinalis, a dominant component of the Sundarban mangrove forest in Bangladesh to estimate dispersal dynamics over various distances within estuaries. A total of 423 individuals of A. officinalis, distributed over thirteen sites in the Sundarbans were sampled at both large and fine-scaled spatial level, the latter for comparison of natural and dense with fragmented sites. Up to 62 alleles showed a comparable level of allele diversity and gene diversities at both juvenile and adult life stages, assuming overall high levels of outcrossing. Significant inbreeding levels and high kinship values were solely observed at a reforested A. officinalis site. Genetic variables revealed high levels of connectivity between sites at close vicinity, with only a weak structure over larger geographic distances, between natural core Sundarban and coastal edge populations. Kinship coefficients were mostly low though significant up to several kilometers along estuarine branches, thereby suggesting propagule dispersal beyond each site. A fine-scaled analysis of kinship levels estimated for fragmented and natural forest plots along both sides of a river revealed that fragmentation induced higher kinship levels over 25–50 m distance, whereas dense natural populations were composed of less related individuals at shortest distances. A. officinalis populations might stay resilient through a mixture of unrelated propagules, clearly adhering a migrant pool model in natural sites and propagule pool model in fragmented sites. A poor gene diversity of planted trees might jeopardize the sustainability of mangrove plantation practices. Furthermore, high levels of diversity and connectivity indicated that the core Sundarban populations are a relevant source of germplasm in afforestation programmes.

Basyuni M, Sagami H, Baba S, Oku H. 2019. Response of polyisoprenoid concentration and profile in three groups of mangrove seedlings of coping with long-term salinity. Biodiversitas 20: 320-326. The response of polyisoprenoid (polyprenol and dolichol) concentration and distribution was investigated with three groups mechanism of coping with salinity: a secreting mangrove species of Avicennia officinalis, a non-secreting species (excluder) of Bruguiera cylindrica, and a salt-accumulating species of Xylocarpus granatum. The seedlings of three mangroves were grown under 0 and 3% salinity concentration for five months. Polyisoprenoids in the lipid extracts were examined by two-dimensional thin layer chromatography (2D-TLC). The pattern of the polyprenols and dolichols in the leaves and roots were categorized as two types (I and II). In category I, dolichols dominated over polyprenols, however, in category II, the existence of both polyprenols and dolichols was found. In the leaves, type-I was observed in A. officinalis under 0 and 3% salinity. On the other hand, type-II was found in B. cylindrica and X. granatum under 0 and 3% salt concentrations. A similar pattern was found in the roots, A. officinalis (type-I), and that in B. cylindrica and X. granatum was type-II. This finding depicted that the seedlings of A. officinalis, B. cylindrica, and X. granatum leaves and roots imply no change in the distribution type: the categories were distributed as type I or II under 0% salt concentrations, as well as type-I or II under 3% salt concentrations. This study implied that polyisoprenoids may play a protective function against salinity in the mangrove leaves and roots of three groups scheme (secreting, excluding, and accumulating) of salt management.

Endophytic fungi, especially from mangrove plants, are rich source of secondary metabolites, which plays a major role in various pharmacological actions preferably in cancer and bacterial infections. To perceive its role in antidiabetic activity we isolated and tested the metabolites derived from a novel strain Alternaria longipes strain VITN14G obtained from mangrove plant Avicennia officinalis. The crude extract was analyzed for antidiabetic activity and subjected to column chromatography. The isolated fractions were screened in vitro for α-glucosidase and α-amylase inhibitory activities. The cytotoxicity of the isolated fractions was studied on L929 cell lines. Following which, the screened fraction 2 was allowed for structure elucidation using gas chromatography-mass spectrometry, one-dimensional, two-dimensional nuclear magnetic resonance spectroscopy, ultraviolet, and Fourier-transform infrared analysis. The binding energies of the isolated fraction 2 with glycolytic enzymes were calculated by molecular docking studies using AutoDock Vina. The isolated fraction 2 identified as 2,4,6-triphenylaniline, showed no significant difference in α-amylase inhibition rates and a significant difference of 10% in α-glucosidase inhibition rates than that of the standard drug acarbose. Further, the cytotoxicity assay of the isolated fraction 2 resulted in a cell viability of 73.96%. Supportingly, in silico studies showed 2,4,6-triphenylaniline to produce a stronger binding affinity toward the glycolytic enzyme targets. The compound 2,4,6-triphenylaniline isolated from A. longipes strain VITN14G exhibited satisfactory antidiabetic activity for type 2 diabetes in vitro, which will further be confirmed by in vivo studies. Successful outcome of the study will result in a natural substitute for existing synthetic antidiabetic drugs.

Nutrient resorption efficiency is an important nutrient conservation and ecophysiological mechanism of mangroves growing in saline environments. This study investigated the nitrogen, phosphorus, and potassium resorption efficiency of Avicennia officinalis L. growing across a salinity gradient with seasonal variations in the Sundarbans of Bangladesh. Due to decreasing salinity during the monsoon and postmonsoon seasons, the nutrient availability in soil and nutrient resorption efficiency did not vary significantly among the low-salinity, medium-salinity, and high-salinity zones. However, the nutrient availability in the medium-salinity and high-salinity zones was significantly lower than that in the low-salinity zone during the premonsoon season due to increased salinity. Consequently, nutrient resorption efficiency in the medium-salinity and high-salinity zones was significantly higher than that in the low-salinity zone during the premonsoon. Further, leaf vein density of A. officinalis in the medium-salinity and high-salinity zones was significantly higher than that in the low-salinity zone. This modification in vein density was the mechanism for the higher nutrient resorption efficiency of A. officinalis in the medium-salinity and high-salinity zones than that in the low-salinity zone. This plasticity in nutrient resorption efficiency is a physiologically adaptive mechanism that enables A. officinalis to persist in increasingly saline environments due to climate change.

Spatial distribution of potentially toxic metals (PTMs) and their accumulation in mangrove Avicennia officinalis L. were studied along 8 locations in and around Sundarban mangrove wetland, India. Among 8 locations, S3 (Chemaguri) and S5 (Ghushighata) showed higher concentration of PTMs (Cd, Cr, Cu, Ni, Pb, Zn) characterized by higher enrichment factors (3.45-10.03), geo-accumulation indices (0.04-1.22), contamination factors (1.14-3.51) and pollution load indices (1.3-1.45) indicating progressive deterioration of estuarine quality and considerable ecotoxicological risk. Metal concentration in A. officinalis leaves showed significant correlation with sediment metals implying elevated level of bioaccumulation. Significant statistical correlation between photosynthetic pigments (Chlorophyll a, Chlorophyll b), antioxidant response (free radical scavenging and reducing ability) and stress enzymatic activity (Peroxidase, Catalase, Super-oxide dismutase) of A. officinalis with increasing metal concentration in the contaminated locations reflects active detoxification mechanism of the plant. The study indicates the potentiality of biomonitoring metal pollution using studied biochemical markers in mangrove habitats.

This aim of the research is to know the condition of the ecology of mangrove forests in the Waisai Raja Ampat District, West Papua. This research was carried out during the year, from April 2012-April 2013. Research locations include three (3) stations which were Moko, Kimindores and the tourist port area. The method was line transect with quadratic plot along a 100-meter at the seaside. The plots were created in size of 1 x 1m 2 ; 5 x 5 m 2 ; 10 x 10 m 2 and 20 x 20 m 2 . The observations showed that there were 8 types of mangrove in the town of Waisai consisting of Avicennia officinalis, Sonneratia alba, Rhizophora stylosa, R. mangle, R. mucronata, Aegricecas floridum, Bruguiera gymnnorhiza , and Nypa fruticans. The distribution of mangrove types in Waisai is spread unevenly. A. floridum was found only at Moko; R. mucronata was at the area of the tourist Port only; and Nypa has a wider distribution since they were found at the three stations of observations. Key words : biota, diversity, mangrove, Raja Ampat.

In a mangrove environment, several groups of organisms are symbiotically associated with pneumatophores (aerial roots). But whether these organisms are exclusively found in this habitat (uniqueness) or accidentally settled here from water or sedimentary habitat, is not clearly understood. This hampers our understanding of their functional ecology. Hence the present study aimed to characterise the epibiotic microalga and meiofauna from the pneumatophores of mangrove Avicennia officinalis . To determine the habitat influence, these communities were also compared with those found in water and sedimentary habitat. Four estuarine mangrove sites represented by brackish water (salinity varying from 5 to 12) and located at different spatial scales (5–50 km distance) were chosen to determine whether these communities are consistent or vary with sites. Overall, the microalgal community was found to vary both among the habitats as well as sites. In the pneumatophore habitat, several tychoplanktons such as Coscinodiscus, Thalassionema , Cyclotella, Fragilariopsis, and Biddulphia were observed instead of benthic ones. These diatoms are planktonic genera and might have settled on pneumatophores from the water column. The observed variability in the microalgal composition among the sites is possibly due to predatory interactions or the dominance of particular species of macroalga that govern the diatom community. On the other hand, among the meiofaunal community, diptera, halacarida, tanaidacea, and foraminifera were mainly found in the pneumatophore habitat than in the sediment. Because pneumatophores either provide refugee to meiofauna against predators or are saturated with oxygen that meiofauna can utilize for respiration. Pneumatophores were also found to harbour several rare (abundance

Avicennia officinalis L. is a typical mangrove species, inhabiting inhospitable environments in the interface between sea and land. In this study, we generated RNA-seq data to de novo assemble the A. officinalis transcriptome. Starting with 36.24 million 100 bp paired-end reads, 38,576 high-confidence transcripts with an average length of 834 bp were produced after filtration of weakly expressed and redundant transcripts. We found known protein homologs for 22,254 of these transcripts, and assigned them to at least one of 119 gene ontology (GO) terms. In addition, we identified different copies and isoforms of three candidate genes, AoPIP, AoTIP and AoDHN1, which might be involved in salt excretion via salt glands. All these genes were highly expressed in leaf tissue of A. officinalis, suggestive of a complicated mechanism of response to salt stress. We further identified 613 microsatellite markers for the assessment of genetic diversity and population differentiation in A. officinalis. Genomic resources generated in this study would be an important foundation for future research into molecular mechanisms underlying salt and other stress tolerance, as well as the evolutionary history of this mangrove species and its relatives.