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Introduction: Oral hygiene instruction performed by different personnel in various manners is a necessity for prevention and treatment of dental caries and periodontal diseases. Since dentists are the majority of personnel who deliver oral hygiene instruction to patients, this study was designed to evaluate dentists' attitude towards oral hygiene instruction in their private office in Yazd. Methods: A questionnaire including demographic information and various oral hygiene instruction methods was prepared and delivered to 151 dentists who were working in their private office in Yazd. Data were analyzed with SPSS 16 software and Fisher exact test was performed. Results: Results revealed that 95.3% of dentists gave oral hygiene instruction to their patients. This training included dental flossing techniques followed by tooth brushing. Therefore, using an oral model was the most common way of patient's instruction. Conclusion: Results obtained from this study showed that the majority of dentists in Yazd gave oral hygiene instruction to their patients through various methods. [ABSTRACT FROM AUTHOR]

Background: The differences between marginal gingiva and interdental papilla may be due to variation in the molecular composition of these two different anatomical structures. The aim of this study was to evaluate the staining intensity of fibronectin in human marginal gingiva and interdental papilla., Materials and Methods: In a prospective analytical study, 16 healthy subjects needing crown lengthening surgery were selected. All participants were medically healthy, non-smokers, with no medication intake, and a healthy periodontium. During surgery, facial/buccal marginal gingiva and interdental papilla were separately harvested. The specimens were subjected to hematoxylin and eosin, histochemical (Masson' strichorom, reticulin, and elastic), and immunohistochemical staining for evaluation of morphology and inflammation; assessment of connective tissue fibers (collagen, reticulin, and elastic); and determination of fibronectin staining intensity. The data were analyzed by Spsssoftware, Wilcoxon, and Spearman tests. P<0.05 was considered to be statistically significant., Results: From a total of 32 specimens, 21 specimens were found to be normal or having mild inflammation, while the remaining specimens had moderate to severe inflammation in some parts. Collagen fibers were found to be dense in reticular connective tissue and degenerated in the region of inflammation. Reticulin fibers strongly stained near epithelium. Elastic fibers were sparsely found. Mean fibronectin staining intensity between marginal gingiva and interdental papilla was not statically significant (P=0.44). There is no statistically significant correlation between tissue inflammation and fibronectin staining intensity (P=0.76 for marginal gingival and P=0.20 for interdental papilla). Considering all specimens, fibronectin staining intensity of connective tissue adjacent to Sulcular/Junctional epithelium was higher than reticular connective tissue (P=0.003) and higher than connective tissue adjacent to oral epithelium (P<0.001)., Conclusion: This study did not show any difference in interdental papilla and marginal gingival with respect to fibronectin composition. More studies in this context are needed.